Abstract：
:Fumonisins (FBs) exist widely in crops, foods and feeds. Consumption of FBs contaminated corn can cause oesophageal cancer. So it is necessary to develop sensitivity methods for its detection. Here, we report an enhanced assay for rapid and ultra-sensitive detection of FBs based on nanomagnetic beads (NMBs) and antibody-biotin-streptavidin-HRP. Because antibody-BNHS can bind with several number of streptavidin-HRP, the signal amplification of the catalytical oxidation of TMB was enhanced. The detection limit of sensor was down to 0.21 ng mL-1 with a linear range from 0.31 to 162.42 ng mL-1. Since NMBs provide a nearly "in solution" reaction, they lead to a shortened reaction time (22 min) than that of flat solid-phase based traditional assay. Furthermore, the recoveries obtained by standard FBs spiked to maize samples were from 100.6 to 107.3%. This enhanced assay supplied a rapid, sensitive and reliable method for detection of FBs in maize.
journal_name
Food Chemjournal_title
Food chemistryauthors
Yang H,Zhang Q,Liu X,Yang Y,Yang Y,Liu M,Li P,Zhou Ydoi
10.1016/j.foodchem.2020.126356subject
Has Abstractpub_date
2020-06-30 00:00:00pages
126356eissn
0308-8146issn
1873-7072pii
S0308-8146(20)30213-2journal_volume
316pub_type
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