Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.


:Fumonisins (FBs) exist widely in crops, foods and feeds. Consumption of FBs contaminated corn can cause oesophageal cancer. So it is necessary to develop sensitivity methods for its detection. Here, we report an enhanced assay for rapid and ultra-sensitive detection of FBs based on nanomagnetic beads (NMBs) and antibody-biotin-streptavidin-HRP. Because antibody-BNHS can bind with several number of streptavidin-HRP, the signal amplification of the catalytical oxidation of TMB was enhanced. The detection limit of sensor was down to 0.21 ng mL-1 with a linear range from 0.31 to 162.42 ng mL-1. Since NMBs provide a nearly "in solution" reaction, they lead to a shortened reaction time (22 min) than that of flat solid-phase based traditional assay. Furthermore, the recoveries obtained by standard FBs spiked to maize samples were from 100.6 to 107.3%. This enhanced assay supplied a rapid, sensitive and reliable method for detection of FBs in maize.


Food Chem


Food chemistry


Yang H,Zhang Q,Liu X,Yang Y,Yang Y,Liu M,Li P,Zhou Y




Has Abstract


2020-06-30 00:00:00












  • Quality and antioxidant properties of a reduced-sugar pomegranate juice jelly with an aqueous extract of pomegranate peels.

    abstract::In the present study, the production of a reduced-sugar pomegranate juice jelly supplemented with an aqueous extract of pomegranate peel (PE) is described. Influence of different carbohydrate polymers (guar (G), xanthan (X) and tragacanth (T) gums) on rheological properties was studied. Combination GXT presented the m...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Ventura J,Alarcón-Aguilar F,Roman-Ramos R,Campos-Sepulveda E,Reyes-Vega ML,Daniel Boone-Villa V,Jasso-Villagómez EI,Aguilar CN

    更新日期:2013-01-01 00:00:00

  • Determination of colistin in animal tissues, egg, milk, and feed by ultra-high performance liquid chromatography-tandem mass spectrometry.

    abstract::A confirmatory method for the determination of colistin in animal tissues, egg, milk, and feed was developed and validated. Colistin A and colistin B were extracted from samples with the mixture of 10% trichloroacetic acid-acetonitrile and isolated with mixed-mode weak cation exchange cartridge. Analytes were separate...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Fu Q,Li X,Zheng K,Ke Y,Wang Y,Wang L,Yu F,Xia X

    更新日期:2018-05-15 00:00:00

  • Phosphoproteome analysis of sarcoplasmic and myofibrillar proteins in bovine longissimus muscle in response to postmortem electrical stimulation.

    abstract::Protein phosphorylation changes of the sarcoplasmic and myofibrillar proteins in beef longissimus muscle in response to electrical stimulation (ES) was investigated. Sarcoplasmic and myofibrillar proteins purified from muscle samples taken at 0, 3 and 10h after ES were separated on SDS-PAGE and stained with phosphorou...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Li C,Zhou G,Xu X,Lundström K,Karlsson A,Lametsch R

    更新日期:2015-05-15 00:00:00

  • Recent applications of covalent organic frameworks and their multifunctional composites for food contaminant analysis.

    abstract::Food safety issues are a great concern of the international community. Many methods to analyze and detect food contaminants have been widely researched because both biological and chemical contaminants in food can cause severe damage to human health. Several adsorbent materials have been utilized in the complicated pr...

    journal_title:Food chemistry

    pub_type: 杂志文章,评审


    authors: Xin J,Wang X,Li N,Liu L,Lian Y,Wang M,Zhao RS

    更新日期:2020-11-15 00:00:00

  • Microfiltered red-purple pitaya colorant: UPLC-ESI-QTOF-MSE-based metabolic profile and its potential application as a natural food ingredient.

    abstract::Complete characterization of microfiltered red-purple pitaya colorant (MRPPC) and its potential applications in foods is described. Using sensorial analysis, products that use carmine or beetroot dye as a food colorant in their formulations were compared. The effect of storage under refrigeration on the microbiologica...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Lima ACV,Dionisio AP,Abreu FAP,Silva GSD,Lima Junior RD,Magalhães HCR,Garruti DDS,Araújo IMDS,Artur AG,Taniguchi CAK,Rodrigues MDCP,Zocolo GJ

    更新日期:2020-11-15 00:00:00

  • Identification and quantification of phenolic compounds of selected fruits from Madeira Island by HPLC-DAD-ESI-MS(n) and screening for their antioxidant activity.

    abstract::Five fruits species commonly cultivated and consumed in Madeira Island (Portugal) were investigated for their phenolic profile by means of reversed phase high-performance liquid chromatography coupled to diode array detection and electrospray ionisation mass spectrometry (HPLC-DAD-ESI/MS(n)) and antioxidant potential....

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Spínola V,Pinto J,Castilho PC

    更新日期:2015-04-15 00:00:00

  • Food composition activities in South Africa.

    abstract::Researchers at the South African Medical Research Council used the Bangkok Declaration, Thailand, 2009, as a guideline for their food composition activities. The vision is to build a comprehensive food composition database for the country. Activities are directed at increasing the number of food items with country-spe...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Wolmarans P,Chetty J,Danster-Christians N

    更新日期:2013-10-01 00:00:00

  • Isolation and identification of polysaccharides from Pythium arrhenomanes and application to strawberry fruit (Fragaria ananassa Duch.) preservation.

    abstract::In this study, we extracted exopolysaccharides from Pythium arrhenomanes and purified them to obtain three polysaccharides (PEPS-1, PEPS-2, PEPS-3). The composition of these polysaccharides was determined. PEPS-2 and PEPS-3 showed outstanding antioxidant activities. PEPS-2 was chosen as a polysaccharide-based coating ...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Yuan Z,Shi Y,Cai F,Zhao J,Xiong Q,Wang Y,Wang X,Zheng Y

    更新日期:2020-03-30 00:00:00

  • Characteristics of compounds in hops using cyclic voltammetry, UV-VIS, FTIR and GC-MS analysis.

    abstract::The article presents the antioxidant properties of the extracts of hop EI and EII, by the electrochemical methods on a platinum electrode and comparative analysis of the composition of the extracts of hops using UV-VIS, FTIR and GC-MS methods. The hops extract EI, was obtained from the waste of the hops cone. The hops...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Masek A,Chrzescijanska E,Kosmalska A,Zaborski M

    更新日期:2014-08-01 00:00:00

  • Bioavailability and antioxidant potential of rooibos flavonoids in humans following the consumption of different rooibos formulations.

    abstract::In a complete crossover design, a human study with twelve healthy male volunteers has been conducted using a placebo and different rooibos drinks (rooibos tea and an isolated active fraction) from unfermented rooibos (Aspalathus linearis). Blood and urine samples were collected before and up to 24h after consumption o...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Breiter T,Laue C,Kressel G,Gröll S,Engelhardt UH,Hahn A

    更新日期:2011-09-15 00:00:00

  • Complex enzyme hydrolysis releases antioxidative phenolics from rice bran.

    abstract::In this study, phenolic profiles and antioxidant activity of rice bran were analyzed following successive treatment by gelatinization, liquefaction and complex enzyme hydrolysis. Compared with gelatinization alone, liquefaction slightly increased the total amount of phenolics and antioxidant activity as measured by fe...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Liu L,Wen W,Zhang R,Wei Z,Deng Y,Xiao J,Zhang M

    更新日期:2017-01-01 00:00:00

  • A non-mercury electrode for the voltammetric determination of butralin in foods.

    abstract::A modified electrode was developed with Co-Ag bimetallic nanoparticles stabilized in poly(vinylpyrrolidone) for butralin monitoring. This is the first non-mercury electrode proposed for the quantification of butralin. The bimetallic nanoparticles were characterized by spectroscopic and microscopic techniques, which sh...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Gerent GG,Santana ER,Martins EC,Spinelli A

    更新日期:2021-05-01 00:00:00

  • Chemical features of Pericarpium Citri Reticulatae and Pericarpium Citri Reticulatae Viride revealed by GC-MS metabolomics analysis.

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    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Yi L,Dong N,Liu S,Yi Z,Zhang Y

    更新日期:2015-11-01 00:00:00

  • The impact of aging wine in high and low oxygen conditions on the fractionation of Cu and Fe in Chardonnay wine.

    abstract::Solid-phase extraction has previously been used to fractionate copper and iron into hydrophobic, cationic and residual forms. This study showed the change in fractionated copper and iron in Chardonnay wines with 1-year of bottle aging under variable oxygen and protein concentrations. Wines containing protein in low ox...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Kontoudakis N,Guo A,Scollary GR,Clark AC

    更新日期:2017-08-15 00:00:00

  • Molecular basis and potential applications of capsaicinoids and capsinoids against the elongation of etiolated wheat (Triticum aestivum L.) coleoptiles in foods.

    abstract::Capsaicinoids and capsinoids from dietary peppers have promising sensory properties and bioactivity, but the molecular basis of their penetration mechanism through cell lipid bilayers and its relationship to their bioavailability as food constituents are still poorly understood. Herein, statistically significant linea...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Chen K,Qian Y,Ge Z,Chen H,Qian C,Li Y,Chen Z

    更新日期:2019-12-15 00:00:00

  • Production of low potassium kale with increased glucosinolate content from vertical farming as a novel dietary option for renal dysfunction patients.

    abstract::The production of low potassium vegetables arose out of the dietary needs of patients with renal dysfunction. Attempts have been made to reduce potassium content in vegetables and fruits; however, induced potassium deficiency has often resulted in decreased yields. Here, we investigated a new method of producing low p...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Son YJ,Park JE,Kim J,Yoo G,Lee TS,Nho CW

    更新日期:2021-03-01 00:00:00

  • Effect of calcium chloride on emulsion stability of methyl-esterified citrus pectin.

    abstract::High methyl-esterified citrus pectin (HMCP) molecules could be self-assembled into micelles in water. The morphology of HMCP micelles in water was irregular spheres, long rods, and arc-shaped. Most of HMCP molecules cross-linked with HMCP micelles in the presence of calcium chloride and increased the range of size dis...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Fan C,Chen X,He J

    更新日期:2020-12-01 00:00:00

  • Two alternative multiplex PCRs for the identification of the seven species of anglerfish (Lophius spp.) using an end-point or a melting curve analysis real-time protocol.

    abstract::Anglerfish (Lophius spp.) is consumed worldwide and is an important economic resource though its seven species are often fraudulently interchanged due to their different commercial value, especially when sold in the form of fillets or pieces. Molecular analysis is the only possible mean to verify traceability and coun...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Castigliego L,Armani A,Tinacci L,Gianfaldoni D,Guidi A

    更新日期:2015-01-01 00:00:00

  • Systematic assessment of oat β-glucan catabolism during in vitro digestion and fermentation.

    abstract::β-Glucan as a component of grain cell walls is consumed daily. However, little is known about whether β-glucan is influenced by the gastrointestinal environment. In this study, we aim to investigate the integrated metabolic process of cereal β-glucan. In vitro simulated digestion and fermentation combined with microbi...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Bai J,Li T,Zhang W,Fan M,Qian H,Li Y,Wang L

    更新日期:2021-01-16 00:00:00

  • A screening method based on UV-Visible spectroscopy and multivariate analysis to assess addition of filler juices and water to pomegranate juices.

    abstract::Consumer demand for pomegranate juice has considerably grown, during the last years, for its potential health benefits. Since it is an expensive functional food, cheaper fruit juices addition (i.e., grape and apple juices) or its simple dilution, or polyphenols subtraction are deceptively used. At present, time-consum...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Boggia R,Casolino MC,Hysenaj V,Oliveri P,Zunin P

    更新日期:2013-10-15 00:00:00

  • Effect of training systems on fatty acids and their derived volatiles in Cabernet Sauvignon grapes and wines of the north foot of Mt. Tianshan.

    abstract::C6 and C9 volatiles, originated from fatty acids, are important volatiles for 'Cabernet Sauvignon' grapes and wines. This study evaluated the influence of different training systems including Modified Vertical Shoot Positioned, (M-VSP); Fan training system with two trunks (F-TT); Fan training system with multiple trun...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Xu XQ,Cheng G,Duan LL,Jiang R,Pan QH,Duan CQ,Wang J

    更新日期:2015-08-15 00:00:00

  • Characterization of phenolics of Sarcandra glabra by non-targeted high-performance liquid chromatography fingerprinting and following targeted electrospray ionisation tandem mass spectrometry/time-of-flight mass spectrometry analyses.

    abstract::In this study, we successfully characterised the phenolic profiles of Sarcandra glabra (Thunb.) Nakai by high-performance liquid chromatography (HPLC) fingerprinting analyses and mass spectrometry (MS) identification. We first established a specific and valid HPLC approach for fingerprint analysis of S. glabra based o...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Zhou H,Liang J,Lv D,Hu Y,Zhu Y,Si J,Wu S

    更新日期:2013-06-15 00:00:00

  • Enzyme immunoassay (ELISA/immunosensor) for a sensitive detection of pork adulteration in meat.

    abstract::ELISA/immunosensor were developed for a sensitive detection of pork adulteration in meat. Two formats of ELISA were performed. First, an extracted IgG was directly immobilized in the microplate. This assay allowed an identification of 0.01% as level of pork adulteration in 14 h15 min. In order to decrease the time of ...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Mandli J,El Fatimi I,Seddaoui N,Amine A

    更新日期:2018-07-30 00:00:00

  • Differentiation of degrees of ripeness of Catuai and Tipica green coffee by chromatographical and statistical techniques.

    abstract::The quality of green coffee is influenced by the degree of ripeness of the fruit at harvest. The aim of this study was to identify chemical markers differentiating between degrees of ripeness. Two coffee varieties, Catuai and Tipica, from the same farm were analysed using the following parameters and methods: caffeine...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Smrke S,Kroslakova I,Gloess AN,Yeretzian C

    更新日期:2015-05-01 00:00:00

  • Bioaccessibility of bioactive compounds after non-thermal processing of an exotic fruit juice blend sweetened with Stevia rebaudiana.

    abstract::A comparative study of the bioaccessibility of bioactive compounds and antioxidant capacity in a fruit juice-Stevia rebaudiana mixture processed by pulsed electric fields (PEF), high voltage electrical discharges (HVED) and ultrasound (USN) technology at two equivalent energy inputs (32-256kJ/kg) was made using an in ...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Buniowska M,Carbonell-Capella JM,Frigola A,Esteve MJ

    更新日期:2017-04-15 00:00:00

  • Spectrophotometric determination of iodate in iodised salt by flow injection analysis.

    abstract::A simple and sensitive flow injection (FI) method for the determination of iodate is proposed. The method is based on the reaction of iodate with hydroxylamine in acidic solution. Sulfanilamide is diazotised by the nascent nitrite and the diazonium ion produced is then coupled with N-(1-naphthyl)ethyenediamine in hydr...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Shabani AMH,Ellis PS,McKelvie ID

    更新日期:2011-11-15 00:00:00

  • Earthy off-flavour in wine: evaluation of remedial treatments for geosmin contamination.

    abstract::Seven treatments (activated charcoal, bentonite, PVPP, yeast cell walls, potassium caseinate, zeolite and grape seed oil) were evaluated for their efficacy in decreasing the concentration of geosmin, responsible for earthy off-flavour in wine. In the red wine the potassium caseinate and grape seed oil treatments decre...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Lisanti MT,Gambuti A,Genovese A,Piombino P,Moio L

    更新日期:2014-07-01 00:00:00

  • Impact of pasteurization of human milk on preterm newborn in vitro digestion: Gastrointestinal disintegration, lipolysis and proteolysis.

    abstract::Human milk feeding is an important recommendation for preterm newborns considering their vulnerability and digestive immaturity. Holder pasteurization (62.5°C, 30min) applied in milk banks modifies its biological quality and its microstructure. We investigated the impact of pasteurization of preterm human milk on its ...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: de Oliveira SC,Bourlieu C,Ménard O,Bellanger A,Henry G,Rousseau F,Dirson E,Carrière F,Dupont D,Deglaire A

    更新日期:2016-11-15 00:00:00

  • Contents of cadmium, mercury and lead in fish from the Atlantic sea (Morocco) determined by atomic absorption spectrometry.

    abstract::As a part of a specific monitoring program, lead (Pb) cadmium (Cd) and mercury (Hg) concentrations in important species of fish from various fishing ports of the southern Kingdom of Morocco (Sardina pilchardus, Scomber scombrus, Plectorhinchus mediterraneus, Trachurus trachurus, Octopus vulgaris, Boops boops, Sarda sa...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Chahid A,Hilali M,Benlhachimi A,Bouzid T

    更新日期:2014-03-15 00:00:00

  • Induction of phenolic metabolites and physiological changes in chamomile plants in relation to nitrogen nutrition.

    abstract::Alternative tools, such as the manipulation of mineral nutrition, may affect secondary metabolite production and thus the nutritional value of food/medicinal plants. We studied the impact of nitrogen (N) nutrition (nitrate/NO3(-) or ammonium/NH4(+) nitrogen) and subsequent nitrogen deficit on phenolic metabolites and ...

    journal_title:Food chemistry

    pub_type: 杂志文章


    authors: Kováčik J,Klejdus B

    更新日期:2014-01-01 00:00:00