Abstract：
:Charantin, a steroidal glycoside, exists as a mixture of stigmasterol glucoside (STG) and β-sitosterol glucoside (BSG) in the fruits of Momordica charantia. Charantin has anti-diabetic activity comparable to insulin. The present work discusses a method for separation of components of charantin namely STG and BSG by simple extraction technique followed by preparative HPLC. The identity of separated components was established by chromatographic as well as spectral techniques. Also reversed phase HPLC-DAD method was developed and validated for estimation of STG and BSG present in fruits of Momordica charantia. The method used C18 column (75 mm × 4.6 mm, 3.5 µm) as stationary phase and methanol: water (98:02, v/v) as mobile phase. Retention times of STG and BSG were found to be 10.707 min and 11.870 min, respectively. The validated method was applied to evaluate content of these components in different extracts and some commercial herbal formulations containing fruits of Momordica charantia.
journal_name
Food Chemjournal_title
Food chemistryauthors
Desai S,Tatke P,Mane T,Gabhe Sdoi
10.1016/j.foodchem.2020.128717subject
Has Abstractpub_date
2020-11-25 00:00:00pages
128717eissn
0308-8146issn
1873-7072pii
S0308-8146(20)32579-6journal_volume
345pub_type
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