Abstract：
:Orientin, vitexin and other flavone C-glycosides are functional ingredients abundant in trollflowers. In this study, an efficient method for enrichment of these ingredients from the flowers of Trollius chinensis Bunge was developed using macroporous resin. Separation characteristics of six typical macroporous resins were investigated by static adsorption/desorption and dynamic separation experiments, and HPD450 was selected as optimal one. Dynamic adsorption/desorption experiments on HPD450 columns were conducted to obtain the optimal parameters, followed by a scale-up experiment. Six fractions, TC-1-TC-3 together with their acid hydrolysates were further prepared to evaluate their antioxidant capacities by 2,2-diphenyl-1-picrylhydrazyl radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonate) radical cation scavenging assays. They all have notable concentration-dependent antioxidant activities, with TC-1 showing strong antioxidant capacity higher than orientin. The separation process was high-efficient, low-cost and environmental-friendly, which could afford a potential approach for industrial applications.
journal_name
Food Chemjournal_title
Food chemistryauthors
Sun Y,Yuan H,Hao L,Min C,Cai J,Liu J,Cai P,Yang Sdoi
10.1016/j.foodchem.2013.03.030subject
Has Abstractpub_date
2013-11-01 00:00:00pages
533-41issue
1eissn
0308-8146issn
1873-7072pii
S0308-8146(13)00333-6journal_volume
141pub_type
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