Abstract:
:Cytotoxicity and genotoxicity of sodium acetate (SA), sodium diacetate (SDA), and potassium sorbate (PS) was tested on Human Umbilical Vein Endothelial Cells (HUVEC). Cytotoxicity was investigated by MTT assay and flow cytometry analysis, while genotoxicity was evaluated using DNA fragmentation and DAPI staining assays. The growth of treated HUVECs with various concentrations of SA, SDA and PS decreased in a dose-and time-dependent manner. The IC50 of 487.71, 485.82 and 659.96 µM after 24 h and IC50 of 232.05, 190.19 and 123.95 µM after 48 h of treatment were attained for SA, SDA and PS, respectively. Flow cytometry analysis showed that early and late apoptosis percentage in treated cells was not considerable. Also neither considerable DNA fragmentation nor DNA smear was observed using DAPI staining and DNA ladder assays. Overall, it can be concluded that the aforementioned food additives can be used as safe additives at low concentration in food industry.
journal_name
Food Chemjournal_title
Food chemistryauthors
Mohammadzadeh-Aghdash H,Sohrabi Y,Mohammadi A,Shanehbandi D,Dehghan P,Ezzati Nazhad Dolatabadi Jdoi
10.1016/j.foodchem.2018.03.020subject
Has Abstractpub_date
2018-08-15 00:00:00pages
211-215eissn
0308-8146issn
1873-7072pii
S0308-8146(18)30437-0journal_volume
257pub_type
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