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Green one-step synthesis of carbon quantum dots from orange peel for fluorescent detection of Escherichia coli in milk.

Abstract：

:Carbon quantum dots (CQDs) prepared by a green one-step approach was used for sensitive and selective assay of Escherichia coli O157: H7 (E. coli). CQDs was synthesized from orange peel as a carbon source via a microwave-assisted method. The CQDs displayed strong green fluorescence under excitation wavelength of 420 nm. A fluorescent probe (CQDs-MNPs) for E. coli was fabricated based on CQDs labeled with aptamer (aptamer-CQDs) and magnetic nanoparticles labeled with complementary DNA (cDNA-MNPs). Fluorescent intensity of the CQDs-MNPs was decreased with addition of E. coli. The linearity between fluorescent intensity and E. coli concentration was used for developing a fluorescent method with detecting range of 500-106 CFU/mL and detection limit of 487 CFU/mL. Milk samples contaminated by E. coli were analyzed by this method, and the results agreed with that achieved by plate-counting methods. This fluorescent probe exhibits great potential in guaranteeing food quality and safety.

journal_name

Food Chem

journal_title

Food chemistry

authors

Hu X,Li Y,Xu Y,Gan Z,Zou X,Shi J,Huang X,Li Z,Li Y

doi

10.1016/j.foodchem.2020.127775

subject

Has Abstract

pub_date

2021-03-01 00:00:00

pages

127775

eissn

0308-8146

issn

1873-7072

pii

S0308-8146(20)31637-X

journal_volume

339

pub_type

杂志文章

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