Abstract:
PURPOSE:This study was aimed to determine the impact of hydroxytyrosol (HT), a minor compound found in olive oil, on breast cancer stem cells (BCSCs) and the migration capacity of triple-negative breast cancer (TNBC) cell lines through the alteration of epithelial-to-mesenchymal transition (EMT) and embryonic signaling pathways. METHODS:BCSCs self-renewal was determined by the mammosphere-forming efficiency in SUM159PT, BT549, MDA-MB-231 and Hs578T TNBC cell lines. Flow cytometric analysis of CD44+/CD24-/low and aldehyde dehydrogenase positive (ALDH+) subpopulations, migration by the "wound healing assay", invasion and Western blot of EMT markers and TGFβ signaling were investigated in SUM159PT, BT549 and MDA-MB-231 cell lines. Wnt/β-catenin signaling was assessed by Western blot in BT549 cells expressing WNT1 and MDA-MB-231 cells. Changes in TGFβ activity was determined by SMAD Binding Element (SBE) reporter assay. RESULTS:HT reduced BCSCs self-renewal, ALDH+ (aldehyde dehydrogenase) and CD44+/CD24-/low subpopulations, tumor cell migration and invasion. Consistently, HT suppressed Wnt/β-catenin signaling by decreasing p-LRP6, LRP6, β-catenin and cyclin D1 protein expression and the EMT markers SLUG, ZEB1, SNAIL and VIMENTIN. Finally, HT inhibited p-SMAD2/3 and SMAD2/3 in SUM159PT, BT549 and MDA-MB-231 cells, what was correlated with a less TGFβ activity. CONCLUSION:In conclusion, we report for the first time the inhibitory role of HT on BCSCs and tumor cell migration by targeting EMT, Wnt/β-catenin and TGFβ signaling pathways. Our findings highlight the importance of the chemopreventive compound HT as a novel candidate to be investigated as an alternative targeted therapy for TNBC.
journal_name
Eur J Nutrjournal_title
European journal of nutritionauthors
Cruz-Lozano M,González-González A,Marchal JA,Muñoz-Muela E,Molina MP,Cara FE,Brown AM,García-Rivas G,Hernández-Brenes C,Lorente JA,Sanchez-Rovira P,Chang JC,Granados-Principal Sdoi
10.1007/s00394-018-1864-1subject
Has Abstractpub_date
2019-12-01 00:00:00pages
3207-3219issue
8eissn
1436-6207issn
1436-6215pii
10.1007/s00394-018-1864-1journal_volume
58pub_type
杂志文章abstract:PURPOSE:While the prevalence of children on vegetarian diets is assumed to be on the rise in industrialized countries, there are hardly any representative data available. In general, vegetarian diets are presumed to be healthy; nevertheless, there are concerns as to whether the dietary specifications required during in...
journal_title:European journal of nutrition
pub_type: 杂志文章,评审
doi:10.1007/s00394-017-1416-0
更新日期:2017-08-01 00:00:00
abstract:PURPOSE:Hepatic fatty acid synthesis is influenced by several nutritional and hormonal factors. In this study, we have investigated the effects of distinct experimental diets enriched in carbohydrate or in fat on hepatic lipogenesis. METHODS:Male Wistar rats were divided into four groups and fed distinct experimental ...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-013-0613-8
更新日期:2014-06-01 00:00:00
abstract:PURPOSE:Resveratrol is a polyphenol present in red wine for which the capability of directly interfering with the hallmark of Alzheimer's disease (AD), i.e. toxic β-amyloid protein (Aβ) aggregation, has been shown recently. Since the stimulation of proteostasis could explain reduced Aβ-aggregation, we searched for prot...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-015-0894-1
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:The only effective and safe treatment of celiac disease (CD) continues being strict exclusion of gluten for life, the so-called gluten-free diet (GFD). Although this treatment is highly successful, following strict GFD poses difficulties to patients in family, social and working contexts, deteriorating his/her ...
journal_title:European journal of nutrition
pub_type: 杂志文章,评审
doi:10.1007/s00394-016-1238-5
更新日期:2017-03-01 00:00:00
abstract:PURPOSE:Adipose tissue is now recognized as a highly active metabolic and endocrine organ. Our aim was to investigate the effect of the dietary fat on the two main adipose tissue functions, endocrine and lipid store, by analyzing the adipose tissue gene expression from metabolic syndrome patients. METHODS:A randomized...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-013-0570-2
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Diet has an important role in host-microbiome interplay, which may result in intestinal permeability changes and physiopathological effects at a systemic level. Despite the importance of maternal microbiota as the main contributor to the initial microbial seeding, little is known about the effects of materna...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-020-02337-7
更新日期:2020-07-29 00:00:00
abstract:PURPOSE:Dietary intakes are reflected in plasma by the presence of hundreds of exogenous metabolites and variations in endogenous metabolites. The exploration of diet-related plasma metabolic profiles could help to better understand the impact of overall diet on health. Our aim was to identify metabolomic signatures re...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-020-02177-5
更新日期:2020-12-01 00:00:00
abstract:PURPOSE:Lactobacillus plantarum Lp62 is a lactic acid bacteria strain that has been isolated from cocoa beans and exhibited probiotic potential. The influence of oral administration of L. plantarum Lp62 on the growth of rat's pups; on yield, cytokines and milk composition was studied. METHODS:Lactobacillus plantarum L...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-018-1628-y
更新日期:2019-04-01 00:00:00
abstract:PURPOSE:Pregnancy is characterised by increased bone turnover, but high bone turnover with resorption exceeding formation may lead to negative maternal bone remodelling. Recent studies are conflicting regarding the effect of calcium on skeletal health in pregnancy. The aim of this study was to examine the seasonal effe...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-017-1385-3
更新日期:2018-04-01 00:00:00
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journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-020-02451-6
更新日期:2021-01-02 00:00:00
abstract::The potential impact on health of diets rich in free sugars, and particularly fructose, is of major concern. The focus of this review is the impact of these sugars on insulin resistance and obesity, and the associated risk of developing type 2 diabetes. Much of the concern is focussed on specific metabolic effects of ...
journal_title:European journal of nutrition
pub_type: 杂志文章,评审
doi:10.1007/s00394-016-1340-8
更新日期:2016-11-01 00:00:00
abstract:PURPOSE:Atherosclerosis is known to be an inflammatory disease. Dendritic cells (DCs) are essential for the regulation of the immune system. Up to 10% of the cells in atherosclerotic plaques are DCs. The cardiovascular protective effects of flavonoids (tea, wine) may be mediated by anti-inflammatory mechanisms that aff...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-010-0125-8
更新日期:2011-04-01 00:00:00
abstract:PURPOSE:Fruit and vegetable intake has been inversely associated with the risk of hypertension; however, there is inconsistent evidence on the long-term association. Given this gap in the literature, it is necessary to identify evidence from large prospective studies, especially in China, where insufficient evidence ex...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-018-1687-0
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Conjugated linoleic acid (CLA) has received great attention in recent years because of its pleiotropic biological activities, but considerably fewer studies have been published addressing its role in serum lipids and atherosclerosis compared to other topics covered. AIMS OF THE STUDY:The aim of the present ...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-007-0653-z
更新日期:2007-06-01 00:00:00
abstract:PURPOSE:Our aim was to characterize the effect of an unfamiliar high-fat diet (HFD) on circadian feeding behaviour, plasma parameters, body weight (BW), and gene expression in the prefrontal cortex (PFC) of adolescent male mice. To this end, mice were allowed to consume a HFD during 48 h, but one group was allowed a fr...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-016-1225-x
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND:Antioxidant defence has been reported to decrease, and oxidative stress to increase, after oral glucose loading in both normal and diabetic subjects. If confirmed in normal subjects, glucose-induced antioxidant depletion has important implications for health in relation to the modern, sugar-rich diet. AIM O...
journal_title:European journal of nutrition
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00394-004-0518-7
更新日期:2005-06-01 00:00:00
abstract:PURPOSE:Epidemiological studies directly examining the association between habitual yoghurt consumption and mental health remain scarce. The aim of this study is to investigate the association of yoghurt consumption with depressive symptoms in adults. METHODS:This is a cross-sectional study of 19,596 Chinese adults (m...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-017-1532-x
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Neurodevelopmental effects of omega-3 fatty acids and mercury from fish consumption have been characterized in children. In contrast, neurobehavioral outcomes associated with fish are not well studied in adults. OBJECTIVE:This study of avid seafood consumers on Long Island (NY, USA) sought to define associa...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-015-0890-5
更新日期:2016-03-01 00:00:00
abstract:PURPOSE:Several studies in recent years have investigated the relationship between alcohol intake and melanoma risk, with conflicting results. To help clarify this issue, we conducted a literature review and dose-response meta-analysis of studies published until June 30th, 2017, that examined the association between al...
journal_title:European journal of nutrition
pub_type: 杂志文章,meta分析
doi:10.1007/s00394-018-1613-5
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:No previous study has examined the associations between egg consumption and prevalence of psychological disorders. The present study was conducted to determine the association between egg consumption and depression in a large sample of Iranian adults. METHODS:This cross-sectional study was conducted on 3172 ad...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-018-1739-5
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:Dietary polyphenols have been reported to have a variety of biological actions, including anticarcinogenic and antioxidant activities. AIM OF THE STUDY:In the present study we investigated the protective effect of dietary polyphenols against N-nitrosodimethylamine (NDMA), N-nitrosopyrrolidine (NPYR) and ben...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-008-0751-6
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND:Inulin-type fructans or chains with mainly beta[2-1] linked fructose molecules escape the ingestion procedure in the small intestine and are fermented by the microflora, and are known to increase colonic absorption of minerals in animals. The fermentation rate in the large bowel into short-chain fatty acids ...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-003-0390-x
更新日期:2003-04-01 00:00:00
abstract:PURPOSE:Diabetes is a chronic inflammatory disorder resulting in endothelial dysfunction which contributes to peripheral arterial disease and limb ischemia. Leukocytes play critical roles in vascular and tissue remodelling after ischemia. This study investigated the effects of dietary glutamine (GLN) supplementation on...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-019-01951-4
更新日期:2020-04-01 00:00:00
abstract:PURPOSE:To investigate whether early introduction of complementary foods (CF) is associated with an increased risk of overweight during childhood, and whether this association differs between formula-fed and breastfed infants. METHODS:We included 2611 participants that were born at term from a Dutch population-based b...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-018-1639-8
更新日期:2018-08-01 00:00:00
abstract:PURPOSE:Walnuts (Juglans regia) are known to have anti-cancer and immunomodulatory effects. However, little information is available on the effects of walnut phenolic extract (WPE) on intestinal inflammation and colitis-associated colon cancer. METHODS:COLO205 cells were pretreated with WPE and then stimulated with tu...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-018-1704-3
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:The study aimed to investigate the potential nephroprotective effects of vitamin D3 in metabolic syndrome (MetS) and the molecular basis of the underlying mechanisms of its action. METHODS:MetS was induced in adult male Wistar rats by adding fructose (10%) to every day drinking water and salt (3%) to the diet...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-020-02249-6
更新日期:2020-04-22 00:00:00
abstract:PURPOSE:Advanced glycation endproducts (AGEs) are produced endogenously and also enter the body during the consumption of AGEs present in heat-processed food. It is unknown whether AGEs of dietary origin accumulate within the body of healthy individuals. AGEs can deposit within skin tissue long-term by crosslinking ext...
journal_title:European journal of nutrition
pub_type: 杂志文章
doi:10.1007/s00394-017-1495-y
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:Lutein is a carotenoid that may play a role in eye health. Human milk typically contains higher concentrations of lutein than infant formula. Preliminary data suggest there are differences in serum lutein concentrations between breastfed and formula-fed infants. AIM OF THE STUDY:To measure the serum lutein ...
journal_title:European journal of nutrition
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00394-009-0047-5
更新日期:2010-02-01 00:00:00
abstract:PURPOSE:Heme iron may contribute to the development of atherosclerosis by catalyzing production of hydroxyl-free radicals and promoting low-density lipoprotein oxidation. However, epidemiologic findings regarding the association between heme iron intake and risk of coronary heart disease (CHD) are inconsistent. We aime...
journal_title:European journal of nutrition
pub_type: 杂志文章,meta分析
doi:10.1007/s00394-013-0535-5
更新日期:2014-01-01 00:00:00
abstract:INTRODUCTION:The objective of this paper was to evaluate the relationship between urinary concentrations of InsP6, bone mass loss and risk fracture in postmenopausal women. MATERIALS AND METHODS:A total of 157 postmenopausal women were included in the study: 70 had low (≤0.76 μM), 42 intermediate (0.76-1.42 μM) and 45...
journal_title:European journal of nutrition
pub_type: 临床试验,杂志文章
doi:10.1007/s00394-012-0377-6
更新日期:2013-03-01 00:00:00