Abstract:
:Many small-interfering (si)RNAs are toxic to cancer cells through a 6mer seed sequence (positions 2-7 of the guide strand). Here we performed an siRNA screen with all 4096 6mer seeds revealing a preference for guanine in positions 1 and 2 and a high overall G or C content in the seed of the most toxic siRNAs for four tested human and mouse cell lines. Toxicity of these siRNAs stems from targeting survival genes with C-rich 3'UTRs. The master tumor suppressor miRNA miR-34a-5p is toxic through such a G-rich 6mer seed and is upregulated in cells subjected to genotoxic stress. An analysis of all mature miRNAs suggests that during evolution most miRNAs evolved to avoid guanine at the 5' end of the 6mer seed sequence of the guide strand. In contrast, for certain tumor-suppressive miRNAs the guide strand contains a G-rich toxic 6mer seed, presumably to eliminate cancer cells.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Gao QQ,Putzbach WE,Murmann AE,Chen S,Sarshad AA,Peter JM,Bartom ET,Hafner M,Peter MEdoi
10.1038/s41467-018-06526-1subject
Has Abstractpub_date
2018-10-29 00:00:00pages
4504issue
1issn
2041-1723pii
10.1038/s41467-018-06526-1journal_volume
9pub_type
杂志文章abstract::Cytosolic HSP90 requires multiple cochaperones in folding client proteins. However, the function of gp96 (HSP90b1, grp94), an HSP90 paralogue in the endoplasmic reticulum (ER), is believed to be independent of cochaperones. Here, we demonstrate that gp96 chaperones multiple Toll-like receptors (TLRs), but not TLR3, in...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms1070
更新日期:2010-09-21 00:00:00
abstract::Different symmetry breaking ways determine various magnetization switching modes driven by spin-orbit torques (SOT). For instance, an applied or effective field parallel to applied current is indispensable to switch magnetization with perpendicular anisotropy by SOT. Besides of this mode, here we experimentally demons...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-08181-y
更新日期:2019-01-16 00:00:00
abstract::The turnover of messenger RNAs (mRNAs) is a key regulatory step of gene expression in eukaryotic cells. Due to the complexity of the mammalian degradation machinery, the contribution of decay factors to the directionality of mRNA decay is poorly understood. Here we characterize a molecular tool to interrogate mRNA tur...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13691
更新日期:2016-12-05 00:00:00
abstract::Cell biologists generally consider that microtubules and actin play complementary roles in long- and short-distance transport in animal cells. On the contrary, using melanosomes of melanocytes as a model, we recently discovered that the motor protein myosin-Va works with dynamic actin tracks to drive long-range organe...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17212-6
更新日期:2020-07-13 00:00:00
abstract::The degradation of intrinsic properties of graphene during the transfer process constitutes a major challenge in graphene device fabrication, stimulating the need for direct growth of graphene on dielectric substrates. Previous attempts of metal-induced transformation of diamond and silicon carbide into graphene suffe...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12099
更新日期:2016-07-04 00:00:00
abstract::Due to the non-flammable nature of water-based electrolytes, aqueous lithium-ion batteries are resistant to catching fire. However, they are not immune to the risk of explosion, since the sealing structure adopted by current batteries limits the dissipation of heat and pressure within the cells. Here, we report a safe...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16460-w
更新日期:2020-05-26 00:00:00
abstract::The gravitational collapse of eruption columns generates ground-hugging pyroclastic density currents (PDCs) with highly variable temperatures, high enough to be a threat for communities surrounding volcanoes. The reasons for such great temperature variability are debated in terms of eruptive versus transport and empla...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10337-3
更新日期:2019-06-06 00:00:00
abstract::Τhe morphology, physiology and immunology, of solid tumors exhibit spatial heterogeneity which complicates our understanding of cancer progression and therapy response. Understanding spatial heterogeneity necessitates high resolution in vivo imaging of anatomical and pathophysiological tumor information. We introduce ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09081-5
更新日期:2019-03-13 00:00:00
abstract::RNA-binding proteins (RBPs) integrate the processing of RNAs into post-transcriptional gene regulation, but the direct contribution of them to myeloid cell specification is poorly understood. Here, we report the first global RBP transcriptomic analysis of myeloid differentiation by combining RNA-seq analysis with myel...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01425-3
更新日期:2017-11-10 00:00:00
abstract::Bird song has historically been considered an almost exclusively male trait, an observation fundamental to the formulation of Darwin's theory of sexual selection. Like other male ornaments, song is used by male songbirds to attract females and compete with rivals. Thus, bird song has become a textbook example of the p...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4379
更新日期:2014-03-04 00:00:00
abstract::The control of water-intake behavior is critical for life because an excessive water intake induces pathological conditions, such as hyponatremia or water intoxication. However, the brain mechanisms controlling water intake currently remain unclear. We previously reported that thirst-driving neurons (water neurons) in...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19191-0
更新日期:2020-11-10 00:00:00
abstract::Plasma membrane Ca2+-ATPases (PMCAs) are key regulators of global Ca2+ homeostasis and local intracellular Ca2+ dynamics. Recently, Neuroplastin (NPTN) and basigin were identified as previously unrecognized obligatory subunits of PMCAs that dramatically increase the efficiency of PMCA-mediated Ca2+ clearance. Here, we...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06075-7
更新日期:2018-09-06 00:00:00
abstract::The effects of high-fat diet (HFD) and postprandial endotoxemia on the development of type 2 diabetes are not fully understood. Here we show that the serine protease prostasin (PRSS8) regulates hepatic insulin sensitivity by modulating Toll-like receptor 4 (TLR4)-mediated signalling. HFD triggers the suppression of PR...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4428
更新日期:2014-03-11 00:00:00
abstract::Carbon-based three-dimensional aerographite networks, built from interconnected hollow tubular tetrapods of multilayer graphene, are ultra-lightweight materials recently discovered and ideal for advanced multifunctional applications. In order to predict the bulk mechanical behaviour of networks it is very important to...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14982
更新日期:2017-04-12 00:00:00
abstract::Topologically protected pseudospin transport, analogous to the quantum spin Hall effect, cannot be strictly implemented for photons and in general bosons because of the lack of symmetry-protected pseudospins. Here we show that the required protection can be provided by the real-space topological excitation of an inter...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06520-7
更新日期:2018-09-28 00:00:00
abstract::A 'pseudogap' was introduced by Mott to describe a state of matter that has a minimum in the density of states at the Fermi level, deep enough for states to become localized. It can arise either from Coulomb repulsion between electrons, and/or incipient charge or spin order. Here we employ ultrafast spectroscopy to st...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7958
更新日期:2015-04-20 00:00:00
abstract::Since the observation of the first brown dwarf in 1995, numerous studies have led to a better understanding of the structures of these objects. Here we present a method for studying material resistivity in warm dense plasmas in the laboratory, which we relate to the microphysics of brown dwarfs through viscosity and e...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9742
更新日期:2015-11-06 00:00:00
abstract::Thousands of tons of isotopic mixtures are processed annually for heavy-water production and tritium decontamination. The existing technologies remain extremely energy intensive and require large capital investments. New approaches are needed to reduce the industry's footprint. Recently, micrometre-size crystals of gr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15215
更新日期:2017-05-09 00:00:00
abstract::During centriole duplication, a single daughter centriole is formed next to the mother centriole. The molecular mechanism that determines a single duplication site remains a long-standing question. Here, we show that intrinsic self-organization of Plk4 is implicated in symmetry breaking in the process of centriole dup...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09847-x
更新日期:2019-04-18 00:00:00
abstract::Previous studies have reported that chromosome synteny in Lepidoptera has been well conserved, yet the number of haploid chromosomes varies widely from 5 to 223. Here we report the genome (393 Mb) of the Glanville fritillary butterfly (Melitaea cinxia; Nymphalidae), a widely recognized model species in metapopulation ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5737
更新日期:2014-09-05 00:00:00
abstract::Shaping ceramics into complex and intricate geometries using cost-effective processes is desirable in many applications but still remains an open challenge. Inspired by plant seed dispersal units that self-fold on differential swelling, we demonstrate that self-shaping can be implemented in ceramics by programming the...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13912
更新日期:2016-12-23 00:00:00
abstract::Selective thermal emission in a useful range of energies from a material operating at high temperatures is required for effective solar thermophotovoltaic energy conversion. Three-dimensional metallic photonic crystals can exhibit spectral emissivity that is modified compared with the emissivity of unstructured metals...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3630
更新日期:2013-01-01 00:00:00
abstract::In the original version of this Article, an additional double-headed arrow was inadvertently included within Fig. 3e. This error has been corrected in both the PDF and HTML versions of the Article. ...
journal_title:Nature communications
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41467-019-08615-1
更新日期:2019-02-12 00:00:00
abstract::Patterns in biomass production are determined by resource input (productivity) and trophic transfer efficiency. At fixed resource input, variation in consumer biomass production has been related to food quality, metabolic type and diversity among species. In contrast, intraspecific variation in individual body size be...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7441
更新日期:2015-03-04 00:00:00
abstract::Writing artificial logic and dynamic function into complex cellular background to achieve desired phenotypes or improved outputs calls for the development of new genetic tools as well as their innovative use. In this study, we present a sensor-regulator and RNAi-based bifunctional dynamic control network that can prov...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05466-0
更新日期:2018-08-02 00:00:00
abstract::The molecular mechanisms underlying vascular inflammation and associated inflammatory vascular diseases are not well defined. Here we show that endothelial intracellular adenosine and its key regulator adenosine kinase (ADK) play important roles in vascular inflammation. Pro-inflammatory stimuli lead to endothelial in...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-00986-7
更新日期:2017-10-16 00:00:00
abstract::The development of malaria parasites throughout their various life cycle stages is coordinated by changes in gene expression. We previously showed that the three-dimensional organization of the Plasmodium falciparum genome is strongly associated with gene expression during its replication cycle inside red blood cells....
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04295-5
更新日期:2018-05-15 00:00:00
abstract::The Cdc45-MCM-GINS (CMG) helicase unwinds DNA during the elongation step of eukaryotic genome duplication and this process depends on the MCM ATPase function. Whether CMG translocation occurs on single- or double-stranded DNA and how ATP hydrolysis drives DNA unwinding remain open questions. Here we use cryo-electron ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10708
更新日期:2016-02-18 00:00:00
abstract::GFI1 is a transcriptional regulator expressed in lymphoid cells, and an "oncorequisite" factor required for development and maintenance of T-lymphoid leukemia. GFI1 deletion causes hypersensitivity to ionizing radiation, for which the molecular mechanism remains unknown. Here, we demonstrate that GFI1 is required in T...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03817-5
更新日期:2018-04-12 00:00:00
abstract::The Satb1 genome organizer regulates multiple cellular and developmental processes. It is not yet clear how Satb1 selects different sets of targets throughout the genome. Here we have used live-cell single molecule imaging and deep sequencing to assess determinants of Satb1 binding-site selectivity. We have found that...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11118-8
更新日期:2019-07-19 00:00:00