Abstract:
:Writing artificial logic and dynamic function into complex cellular background to achieve desired phenotypes or improved outputs calls for the development of new genetic tools as well as their innovative use. In this study, we present a sensor-regulator and RNAi-based bifunctional dynamic control network that can provide simultaneous upregulation and downregulation of cellular metabolism for engineered biosynthesis. The promoter-regulator-mediated upregulation function and its transduced downregulation function through RNAi are systematically verified and characterized. We apply this dynamic control network to regulate the phosphoenolpyruvate metabolic node in Escherichia coli and achieve autonomous distribution of carbon flux between its native metabolism and the engineered muconic acid biosynthetic pathway. This allows muconic acid biosynthesis to reach 1.8 g L-1. This study also suggests the circumstances where dynamic control approaches are likely to take effects.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Yang Y,Lin Y,Wang J,Wu Y,Zhang R,Cheng M,Shen X,Wang J,Chen Z,Li C,Yuan Q,Yan Ydoi
10.1038/s41467-018-05466-0subject
Has Abstractpub_date
2018-08-02 00:00:00pages
3043issue
1issn
2041-1723pii
10.1038/s41467-018-05466-0journal_volume
9pub_type
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