Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis.

Abstract:

:Writing artificial logic and dynamic function into complex cellular background to achieve desired phenotypes or improved outputs calls for the development of new genetic tools as well as their innovative use. In this study, we present a sensor-regulator and RNAi-based bifunctional dynamic control network that can provide simultaneous upregulation and downregulation of cellular metabolism for engineered biosynthesis. The promoter-regulator-mediated upregulation function and its transduced downregulation function through RNAi are systematically verified and characterized. We apply this dynamic control network to regulate the phosphoenolpyruvate metabolic node in Escherichia coli and achieve autonomous distribution of carbon flux between its native metabolism and the engineered muconic acid biosynthetic pathway. This allows muconic acid biosynthesis to reach 1.8 g L-1. This study also suggests the circumstances where dynamic control approaches are likely to take effects.

journal_name

Nat Commun

journal_title

Nature communications

authors

Yang Y,Lin Y,Wang J,Wu Y,Zhang R,Cheng M,Shen X,Wang J,Chen Z,Li C,Yuan Q,Yan Y

doi

10.1038/s41467-018-05466-0

subject

Has Abstract

pub_date

2018-08-02 00:00:00

pages

3043

issue

1

issn

2041-1723

pii

10.1038/s41467-018-05466-0

journal_volume

9

pub_type

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