Abstract:
:Conventional implementations of two-dimensional electronic spectroscopy typically spatially average over ~1010 chromophores spread over ~104 micron square area, limiting their ability to characterize spatially heterogeneous samples. Here we present a variation of two-dimensional electronic spectroscopy that is capable of mapping spatially varying differences in excitonic structure, with sensitivity orders of magnitude better than conventional spatially-averaged electronic spectroscopies. The approach performs fluorescence-detection-based fully collinear two-dimensional electronic spectroscopy in a microscope, combining femtosecond time-resolution, sub-micron spatial resolution, and the sensitivity of fluorescence detection. We demonstrate the approach on a mixture of photosynthetic bacteria that are known to exhibit variations in electronic structure with growth conditions. Spatial variations in the constitution of mixed bacterial colonies manifests as spatially varying peak intensities in the measured two-dimensional contour maps, which exhibit distinct diagonal and cross-peaks that reflect differences in the excitonic structure of the bacterial proteins.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Tiwari V,Matutes YA,Gardiner AT,Jansen TLC,Cogdell RJ,Ogilvie JPdoi
10.1038/s41467-018-06619-xsubject
Has Abstractpub_date
2018-10-11 00:00:00pages
4219issue
1issn
2041-1723pii
10.1038/s41467-018-06619-xjournal_volume
9pub_type
杂志文章abstract::Here we benchmark device-to-device variation in field-effect transistors (FETs) based on monolayer MoS2 and WS2 films grown using metal-organic chemical vapor deposition process. Our study involves 230 MoS2 FETs and 160 WS2 FETs with channel lengths ranging from 5 μm down to 100 nm. We use statistical measures to eval...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20732-w
更新日期:2021-01-29 00:00:00
abstract::Axially chiral compounds play an important role in areas such as asymmetric catalysis. The tyrosine click-like reaction is an efficient approach for synthesis of urazoles with potential applications in pharmaceutical and asymmetric catalysis. Here we discover a class of urazole with axial chirality by restricted rotat...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10677
更新日期:2016-02-11 00:00:00
abstract::Colorectal cancer (CRC) transcriptional subtypes have been recently identified by gene expression profiling. Here we describe an analytical pipeline, microRNA master regulator analysis (MMRA), developed to search for microRNAs potentially driving CRC subtypes. Starting from a microRNA-mRNA tumour expression data set, ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9878
更新日期:2015-11-17 00:00:00
abstract::Although environmental selection and spatial separation have been shown to shape the distribution and abundance of marine microorganisms, the effects of advection (physical transport) have not been directly tested. Here we examine 25 samples covering all major water masses of the Southern Ocean to determine the effect...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3457
更新日期:2013-01-01 00:00:00
abstract::How spatial and temporal information are integrated to determine the direction of cell migration remains poorly understood. Here, by precise microfluidics emulation of dynamic chemoattractant waves, we demonstrate that, in Dictyostelium, directional movement as well as activation of small guanosine triphosphatase Ras ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6367
更新日期:2014-11-06 00:00:00
abstract::Atropisomers are important organic frameworks in bioactive natural products, drugs as well as chiral catalysts. Meanwhile, silanols display unique properties compared to their alcohol analogs, however, the catalytic synthesis of atropisomers bearing silanol groups is challenging. Here, we show a rhodium-catalyzed tors...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18273-3
更新日期:2020-09-07 00:00:00
abstract::The unusual electronic properties of diamondoids, the nanoscale relatives of diamond, make them attractive for applications ranging from drug delivery to field emission displays. Identifying the fundamental origin of these properties has proven highly challenging, with even the most advanced quantum many-body calculat...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3006
更新日期:2013-01-01 00:00:00
abstract::Mitochondrial complex I (CI) deficiency is the most prevalent defect in the respiratory chain in paediatric mitochondrial disease. This heterogeneous group of diseases includes serious or fatal neurological presentations such as Leigh syndrome and there are very limited evidence-based treatment options available. Here...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12317
更新日期:2016-08-09 00:00:00
abstract::Exciton dynamics can be strongly affected by lattice vibrations through electron-phonon coupling. This is rarely explored in two-dimensional magnetic semiconductors. Focusing on bilayer CrI3, we first show the presence of strong electron-phonon coupling through temperature-dependent photoluminescence and absorption sp...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-18627-x
更新日期:2020-09-22 00:00:00
abstract::DNA can form diverse higher-order structures, whose details are greatly dependent on nucleotide sequence. G-rich sequences containing four or more repeats of three guanines are expected to form G-quadruplexes. Here we show that DNA sequences with GGGAGCG repeats found in the regulatory region of the PLEKHG3 gene are c...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6831
更新日期:2014-12-15 00:00:00
abstract::Molecules can efficiently and selectively convert light energy into other degrees of freedom. Disentangling the underlying ultrafast motion of electrons and nuclei of the photoexcited molecule presents a challenge to current spectroscopic approaches. Here we explore the photoexcited dynamics of molecules by an interac...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5235
更新日期:2014-06-23 00:00:00
abstract::The RNA-binding protein RC3H1 (also known as ROQUIN) promotes TNFα mRNA decay via a 3'UTR constitutive decay element (CDE). Here we applied PAR-CLIP to human RC3H1 to identify ∼ 3,800 mRNA targets with >16,000 binding sites. A large number of sites are distinct from the consensus CDE and revealed a structure-sequence ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8367
更新日期:2015-07-14 00:00:00
abstract::Cas9/gRNA-mediated gene-drive systems have advanced development of genetic technologies for controlling vector-borne pathogen transmission. These technologies include population suppression approaches, genetic analogs of insecticidal techniques that reduce the number of insect vectors, and population modification (rep...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19426-0
更新日期:2020-11-03 00:00:00
abstract::Emerging data suggest that locoregional cancer therapeutic approaches with oncolytic viruses can lead to systemic anti-tumour immunity, although the appropriate targets for intratumoral immunomodulation using this strategy are not known. Here we find that intratumoral therapy with Newcastle disease virus (NDV), in add...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14340
更新日期:2017-02-13 00:00:00
abstract::Hereditary retinal degenerations (HRDs) are Mendelian diseases characterized by progressive blindness and caused by ultra-rare mutations. In a genomic screen of 331 unrelated Japanese patients, we identify a disruptive Alu insertion and a nonsense variant (p.Arg1933*) in the ciliary gene RP1, neither of which are rare...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10746-4
更新日期:2019-06-28 00:00:00
abstract::Genome editing critically relies on selective recognition of target sites. However, despite recent progress, the underlying search mechanism of genome-editing proteins is not fully understood in the context of cellular chromatin environments. Here, we use single-molecule imaging in live cells to directly study the beh...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20672-5
更新日期:2021-01-27 00:00:00
abstract::Nascent polypeptides can induce ribosome stalling, regulating downstream genes. Stalling of ErmBL peptide translation in the presence of the macrolide antibiotic erythromycin leads to resistance in Streptococcus sanguis. To reveal this stalling mechanism we obtained 3.6-Å-resolution cryo-EM structures of ErmBL-stalled...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms12026
更新日期:2016-07-06 00:00:00
abstract::Animal propulsors such as wings and fins bend during motion and these bending patterns are believed to contribute to the high efficiency of animal movements compared with those of man-made designs. However, efforts to implement flexible designs have been met with contradictory performance results. Consequently, there ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4293
更新日期:2014-01-01 00:00:00
abstract::Creating micro/nanostructures on fibers is beneficial for extending the application range of fiber-based devices. To achieve this using thermal fiber drawing is particularly important for the mass production of longitudinally uniform fibers up to tens of kilometers. However, the current thermal fiber drawing technique...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-17674-8
更新日期:2020-07-31 00:00:00
abstract::Hepatocellular carcinoma (HCC) is the most predominant primary malignancy in the liver. Genotoxic and genetic models have revealed that HCC cells are derived from hepatocytes, but where the critical region for tumor foci emergence is and how this transformation occurs are still unclear. Here, hyperpolyploidization of ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20572-8
更新日期:2021-01-28 00:00:00
abstract::GFI1 is a transcriptional regulator expressed in lymphoid cells, and an "oncorequisite" factor required for development and maintenance of T-lymphoid leukemia. GFI1 deletion causes hypersensitivity to ionizing radiation, for which the molecular mechanism remains unknown. Here, we demonstrate that GFI1 is required in T...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03817-5
更新日期:2018-04-12 00:00:00
abstract::The efficient selection and isolation of individual cells of interest from a mixed population is desired in many biomedical and clinical applications. Here we show the concept of using photoswitchable semiconducting polymer dots (Pdots) as an optical 'painting' tool, which enables the selection of certain adherent cel...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11468
更新日期:2016-04-27 00:00:00
abstract::Oxygen contamination is a problem which inevitably occurs during severe plastic deformation of metallic powders by exposure to air. Although this contamination can change the morphology and properties of the consolidated materials, there is a lack of detailed information about the behavior of oxygen in nanocrystalline...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03288-8
更新日期:2018-03-05 00:00:00
abstract::Designing chiral materials to manipulate the biological activities of cells has been an important area not only in chemistry and material science, but also in cell biology and biomedicine. Here, we introduce monolayer plasmonic chiral Au nanoparticle (NP) films modified with L- or D-penicillamine (Pen) to be developed...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02268-8
更新日期:2017-12-08 00:00:00
abstract::Increased somatic mitochondrial DNA (mtDNA) mutagenesis causes premature aging in mice, and mtDNA damage accumulates in the human brain with aging and neurodegenerative disorders such as Parkinson disease (PD). Here, we study the complete spectrum of mtDNA changes, including deletions, copy-number variation and point ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13548
更新日期:2016-11-22 00:00:00
abstract::Multiciliated ependymal (E1) cells line the brain ventricles and are essential for brain homeostasis. We previously identified in the lateral ventricles a rare ependymal subpopulation (E2) with only two cilia and unique basal bodies. Here we show that E2 cells form a distinct biciliated epithelium extending along the ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms13759
更新日期:2017-01-09 00:00:00
abstract::Complex materials often exhibit a hierarchical structure with an intriguing mechanism responsible for the 'propagation' of order from the molecular to the nano- or micro-scale level. In particular, the chirality of biological molecules such as nucleic acids and amino acids is responsible for the helical structure of D...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09862-y
更新日期:2019-04-23 00:00:00
abstract::Development of on-chip integrated carbon-based optoelectronic nanocircuits requires fast and non-invasive structural characterization of their building blocks. Recent advances in synthesis of single wall carbon nanotubes and graphene nanoribbons allow for their use as atomically precise building blocks. However, while...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13728-8
更新日期:2020-01-03 00:00:00
abstract::Scalable, integrative methods to understand mechanisms that link genetic variants with phenotypes are needed. Here we derive a mathematical expression to compute PrediXcan (a gene mapping approach) results using summary data (S-PrediXcan) and show its accuracy and general robustness to misspecified reference sets. We ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03621-1
更新日期:2018-05-08 00:00:00
abstract::A key question in materials science is how fast properties evolve, which relates to the kinetics of phase transformations. In metals, kinetics is primarily connected to diffusion, which for substitutional elements is enabled via mobile atomic-lattice vacancies. In fact, non-equilibrium vacancies are often required for...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12762-w
更新日期:2019-10-18 00:00:00