Current and Emerging Preclinical Approaches for Imaging-Based Characterization of Atherosclerosis.


:Atherosclerotic plaques can remain quiescent for years, but become life threatening upon rupture or disruption, initiating clot formation in the vessel lumen and causing acute myocardial infarction and ischemic stroke. Whether and how a plaque ruptures is determined by its macroscopic structure and microscopic composition. Rupture-prone plaques usually consist of a thin fibrous cap with few smooth muscle cells, a large lipid core, a dense infiltrate of inflammatory cells, and neovessels. Such lesions, termed high-risk plaques, can remain asymptomatic until the thrombotic event. Various imaging technologies currently allow visualization of morphological and biological characteristics of high-risk atherosclerotic plaques. Conventional protocols are often complex and lack specificity for high-risk plaque. Conversely, new imaging approaches are emerging which may overcome these limitations. Validation of these novel imaging techniques in preclinical models of atherosclerosis is essential for effective translational to clinical practice. Imaging the vessel wall, as well as its biological milieu in small animal models, is challenging because the vessel wall is a small structure that undergoes continuous movements imposed by the cardiac cycle as it is adjacent to circulating blood. The focus of this paper is to provide a state-of-the-art review on techniques currently available for preclinical imaging of atherosclerosis in small animal models and to discuss the advantages and limitations of each approach.


Mol Imaging Biol


Vigne J,Thackeray J,Essers J,Makowski M,Varasteh Z,Curaj A,Karlas A,Canet-Soulas E,Mulder W,Kiessling F,Schäfers M,Botnar R,Wildgruber M,Hyafil F,Cardiovascular study group of the European Society of Molecular Imaging.




Has Abstract


2018-12-01 00:00:00














  • Synthesis and Evaluation of Ga-68-Labeled Rhein for Early Assessment of Treatment-Induced Tumor Necrosis.

    abstract:PURPOSE:This study aimed to synthesize a necrosis-avid agent using rhein as a precursor and labeled with gallium-68 (Ga-68) for positron emission tomography/computed tomography (PET/CT) imaging, to evaluate response to anticancer treatment in a mouse model. PROCEDURES:1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraaceti...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Zhang A,Wu T,Bian L,Li P,Liu Q,Zhang D,Jin Q,Zhang J,Huang G,Song S

    更新日期:2020-06-01 00:00:00

  • Global cerebral blood flow in relation to cognitive performance and reserve in subjects with mild memory deficits.

    abstract:PURPOSE:This study was undertaken to explore the mechanisms underlying cognitive reserve in subjects with mild memory deficits by using positron emission tomography (PET). METHODS:Global cerebral blood flow (gCBF) and cerebrovascular reserve (CVR) measurements were performed in 15 elders (5 men, 10 women, 62-84, 71.8 ...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Boles Ponto LL,Magnotta VA,Moser DJ,Duff KM,Schultz SK

    更新日期:2006-11-01 00:00:00

  • Correction to: Metabolic Brain Network Analysis of Hypothyroidism Symptom Based on [18F]FDG-PET of Rats.

    abstract::This article was updated to correct the spelling of Hongkai Wang's name: it is correct as displayed here. ...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章,已发布勘误


    authors: Wang H,Tan Z,Zheng Q,Yu J

    更新日期:2019-02-01 00:00:00

  • Evaluation of PET Imaging Performance of the TSPO Radioligand [18F]DPA-714 in Mouse and Rat Models of Cancer and Inflammation.

    abstract:PURPOSE:Many radioligands have been explored for imaging the 18-kDa translocator protein (TSPO), a diagnostic and therapeutic target for inflammation and cancer. Here, we investigated the TSPO radioligand [(18)F]DPA-714 for positron emission tomography (PET) imaging of cancer and inflammation. PROCEDURES:[(18)F]DPA-71...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Zheng J,Winkeler A,Peyronneau MA,Dollé F,Boisgard R

    更新日期:2016-02-01 00:00:00

  • Imaging of Tumor Spheroids, Dual-Isotope SPECT, and Autoradiographic Analysis to Assess the Tumor Uptake and Distribution of Different Nanobodies.

    abstract:PURPOSE:Recent studies have shown rapid accumulation of nanobodies (NBs) in tumors and fast clearance of the unbound fraction, making NBs exceptional tracers for cancer imaging. In this study, we investigate the combination of in vitro imaging of tumor spheroids, in vivo dual-isotope single-photon emission computed tom...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Beltrán Hernández I,Rompen R,Rossin R,Xenaki KT,Katrukha EA,Nicolay K,van Bergen En Henegouwen P,Grüll H,Oliveira S

    更新日期:2019-12-01 00:00:00

  • Molecular imaging of EGFR kinase activity in tumors with 124I-labeled small molecular tracer and positron emission tomography.

    abstract::Positron emission tomography (PET) with epidermal growth factor receptor (EGFR) kinase-specific radiolabeled tracers could provide the means for noninvasive and repetitive imaging of heterogeneity of EGFR expression and signaling activity in tumors in individual patients before and during therapy with EGFR signaling i...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Pal A,Glekas A,Doubrovin M,Balatoni J,Namavari M,Beresten T,Maxwell D,Soghomonyan S,Shavrin A,Ageyeva L,Finn R,Larson SM,Bornmann W,Gelovani JG

    更新日期:2006-09-01 00:00:00

  • Myocardial viability in patients with ischemic cardiomyopathy-evaluation by 3-D integration of myocardial scintigraphic data--and coronary angiographic data.

    abstract:PURPOSE:To determine the prevalence of viable myocardium in patients with ischemic cardiomyopathy and, to evaluate the value of three-dimensional (3-D) fusion imaging of myocardial scintigraphic and angiographic data to assign coronary artery lesions to the corresponding viable and nonviable myocardial territory. PROC...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Schindler TH,Nitzsche EU,Magosaki N,Mix M,Facta AD,Prior JO,Solzbach U,Schelbert HR,Just H

    更新日期:2004-05-01 00:00:00

  • The effect of insulin on the intracellular distribution of 14(R,S)-[18F]Fluoro-6-thia-heptadecanoic acid in rats.

    abstract:PURPOSE:The aim of this study was to determine the effect of hyperinsulinemia on myocardial and hepatic distribution and metabolism of 14(R,S)-[18F]fluoro-6-thia-heptadecanoic acid ([18F]FTHA). PROCEDURES:Mitochondrial retention and intracellular lipid incorporation of [18F]FTHA were compared to that of [14C]-2-bromop...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Ci X,Frisch F,Lavoie F,Germain P,Lecomte R,van Lier JE,Bénard F,Carpentier AC

    更新日期:2006-07-01 00:00:00

  • Noninvasive Imaging for Assessment of the Efficacy of Therapeutic Agents for Hepatocellular Carcinoma.

    abstract::Morphological imaging techniques are typically used in the anti-cancer drug efficacy evaluation process. However, these techniques can evaluate the therapeutic efficacy only when the tumor shows anatomic changes-usually at later stages, when the therapeutic effects are poor. In contrast, molecular imaging allows nonin...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章,评审


    authors: Liang Q,Kong L,Zhu X,Du Y,Tian J

    更新日期:2020-12-01 00:00:00

  • Transport across the blood-brain barrier: stereoselectivity and PET-tracers.

    abstract::This article reviews positron emission tomography (PET) studies of labeled enantiomers of different PET-tracers from the early 1980s. Comparative studies on stereoselective behavior of the transport of tracers across the blood-brain barrier (BBB) are discussed. Tracers are transported through the BBB into the brain, v...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章,评审


    authors: Luurtsema G,de Lange EC,Lammertsma AA,Franssen EJ

    更新日期:2004-09-01 00:00:00

  • Reproducibility of non-invasive a1 adenosine receptor quantification in the rat brain using [(18)F]CPFPX and positron emission tomography.

    abstract:PURPOSE:The A1AR antagonist 8-cyclopentyl-3-(3-fluoropropyl)-1-propylxanthine ([(18)F]CPFPX) has recently been shown to be a suitable radiotracer for quantitative in vivo imaging of the A1 adenosine receptor (A1AR) in rats. The present study evaluates the reproducibility of non-invasive longitudinal A1AR studies with [...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Kroll T,Elmenhorst D,Weisshaupt A,Beer S,Bauer A

    更新日期:2014-10-01 00:00:00

  • Impact of 2-deoxy-2[F-18]fluoro-D-glucose Positron Emission Tomography on the management of patients with advanced melanoma.

    abstract:OBJECTIVES:Accurate staging of patients with melanoma is vital to guide appropriate treatment. 2-Deoxy-2-[F-18]fluoro-D-glucose (FDG)-positron emission tomography (PET) has been reported to be a sensitive and specific technique for the staging of advanced melanoma, however, few studies provide information regarding its...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Harris MT,Berlangieri SU,Cebon JS,Davis ID,Scott AM

    更新日期:2005-07-01 00:00:00

  • Articulated whole-body atlases for small animal image analysis: construction and applications.

    abstract:PURPOSE:Using three publicly available small-animal atlases (Sprague-Dawley rat, MOBY, and Digimouse), we built three articulated atlases and present several applications in the scope of molecular imaging. PROCEDURES:Major bones/bone groups were manually segmented for each atlas skeleton. Then, a kinematic model for e...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Khmelinskii A,Baiker M,Kaijzel EL,Chen J,Reiber JH,Lelieveldt BP

    更新日期:2011-10-01 00:00:00

  • SPECT Imaging of Muscle Injury with [99mTc]MDP in a Mouse Model of Muscular Dystrophy.

    abstract:PURPOSE:Tc-99m methylene diphosphonate ([99mTc]MDP) is an in vivo bone imaging agent that also accumulates in injured skeletal muscle cells. The objective of this study was to investigate if [99mTc]MDP could be used to detect muscle injury in the mdx mouse model of Duchenne muscular dystrophy (DMD). PROCEDURES:Static ...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Gaut C,Sullivan JM,Biscaro B,Soares EJ,Nicholson K,Hoppin J,Verma A

    更新日期:2020-06-01 00:00:00

  • Early assessment of therapeutic response using FDG PET in small cell lung cancer.

    abstract:PURPOSE:We evaluated the ability of 2-deoxy-2-(18)F-fluoro-D-glucose (FDG) positron emission tomography (PET) in the early assessment of therapeutic response in patients with small cell lung cancer (SCLC). PROCEDURES:FDG PET studies were performed before (baseline PET), after the first cycle of chemotherapy (early PET...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Yamamoto Y,Kameyama R,Murota M,Bandoh S,Ishii T,Nishiyama Y

    更新日期:2009-11-01 00:00:00

  • In Vivo MR Imaging of Dual MRI Reporter Genes and Deltex-1 Gene-modified Human Mesenchymal Stem Cells in the Treatment of Closed Penile Fracture.

    abstract:PURPOSE:The purpose of this study was to investigate the feasibility of dual magnetic resonance imaging (MRI) reporter genes, including ferritin heavy subunit (Fth) and transferrin receptor (TfR), which provide sufficient MRI contrast for in vivo MRI tracking, and the Deltex-1 (DTX1) gene, which promotes human mesenchy...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Guo R,Li Q,Yang F,Hu X,Jiao J,Guo Y,Wang J,Zhang Y

    更新日期:2018-06-01 00:00:00

  • [68Ga]Ga-P16-093 as a PSMA-Targeted PET Radiopharmaceutical for Detection of Cancer: Initial Evaluation and Comparison with [68Ga]Ga-PSMA-11 in Prostate Cancer Patients Presenting with Biochemical Recurrence.

    abstract:PURPOSE:This study was undertaken to evaluate radiation dosimetry for the prostate-specific membrane antigen targeted [68Ga]Ga-P16-093 radiopharmaceutical, and to initially assess agent performance in positron emission tomography (PET) detection of the site of disease in prostate cancer patients presenting with biochem...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Green MA,Hutchins GD,Bahler CD,Tann M,Mathias CJ,Territo W,Sims J,Polson H,Alexoff D,Eckelman WC,Kung HF,Fletcher JW

    更新日期:2020-06-01 00:00:00

  • Semiautomated radiosynthesis and biological evaluation of [18F]FEAU: a novel PET imaging agent for HSV1-tk/sr39tk reporter gene expression.

    abstract::2'-deoxy-2'-[(18)F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil ([(18)F]FEAU) is a promising radiolabeled nucleoside designed to monitor Herpes Simplex Virus Type 1 thymidine kinase (HSV1-tk) reporter gene expression with positron emission tomography (PET). However, the challenging radiosynthesis creates problems fo...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Chin FT,Namavari M,Levi J,Subbarayan M,Ray P,Chen X,Gambhir SS

    更新日期:2008-03-01 00:00:00

  • (18)F-FDG PET/CT imaging detects therapy efficacy of anti-EMMPRIN antibody and gemcitabine in orthotopic pancreatic tumor xenografts.

    abstract:PURPOSE:The objective of this study is to evaluate the therapeutic response to a novel monoclonal antibody targeting human extracellular matrix metalloproteinase inducer (EMMPRIN) in combination with gemcitabine in a pancreatic-tumor xenograft murine model by sequential 2-deoxy-2-[18F]fluoro-D-glucose ((18)F-FDG) posit...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Shah N,Zhai G,Knowles JA,Stockard CR,Grizzle WE,Fineberg N,Zhou T,Zinn KR,Rosenthal EL,Kim H

    更新日期:2012-04-01 00:00:00

  • Differences in transport mechanisms of trans-1-amino-3-[18F]fluorocyclobutanecarboxylic acid in inflammation, prostate cancer, and glioma cells: comparison with L-[methyl-11C]methionine and 2-deoxy-2-[18F]fluoro-D-glucose.

    abstract:PURPOSE:We aimed to elucidate trans-1-amino-3-[(18)F]fluorocyclobutanecarboxylic acid (anti-[(18)F]FACBC) uptake mechanisms in inflammatory and tumor cells, in comparison with those of L-[methyl-(11)C]methionine ([(11)C]Met) and 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG). PROCEDURES:Using carbon-14-labeled tracers...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Oka S,Okudaira H,Ono M,Schuster DM,Goodman MM,Kawai K,Shirakami Y

    更新日期:2014-06-01 00:00:00

  • Non-invasive Standardised Uptake Value for Verification of the Use of Previously Validated Reference Region for [18F]Flortaucipir and [18F]Florbetapir Brain PET Studies.

    abstract:PURPOSE:The simplified reference tissue model (SRTM) is commonly applied for the quantification of brain positron emission tomography (PET) studies, particularly because it avoids arterial cannulation. SRTM requires a validated reference region which is obtained by baseline-blocking or displacement studies. Once a refe...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: de Vries BM,Timmers T,Wolters EE,Ossenkoppele R,Verfaillie SCJ,Schuit RC,Scheltens P,van der Flier WM,Windhorst AD,van Berckel BNM,Boellaard R,Golla SSV

    更新日期:2021-01-14 00:00:00

  • Quantitative measurement of regional blood flow in hepatic focal nodular hyperplasia. Findings on positron emission tomography.

    abstract::The blood flow through normal liver parenchyma and a pathologically proven hepatic region of focal nodular hyperplasia (FNH) of the lateral lobe, was measured in a single patient in a quantitative manner using regional dynamic positron emission tomography (PET) imaging with O-15 carbon dioxide (steady state) and O-15 ...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Kunishima S,Koh T,Fukuda K,Taniguchi H

    更新日期:2002-05-01 00:00:00

  • Noninvasive reporter gene imaging of human Oct4 (pluripotency) dynamics during the differentiation of embryonic stem cells in living subjects.

    abstract:PURPOSE:Human pluripotency gene networks (PGNs), controlled in part by Oct4, are central to understanding pluripotent stem cells, but current fluorescent reporter genes (RGs) preclude noninvasive assessment of Oct4 dynamics in living subjects. PROCEDURES:To assess Oc4 activity noninvasively, we engineered a mouse embr...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Ahn BC,Parashurama N,Patel M,Ziv K,Bhaumik S,Yaghoubi SS,Paulmurugan R,Gambhir SS

    更新日期:2014-12-01 00:00:00

  • PET Imaging of HER2-Positive Tumors with Cu-64-Labeled Affibody Molecules.

    abstract:PURPOSE:Previous studies has demonstrated the utility of human epidermal growth factor receptor type 2 (HER2) as an attractive target for cancer molecular imaging and therapy. An affibody protein with strong binding affinity for HER2, ZHER2:342, has been reported. Various methods of chelator conjugation for radiolabeli...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Qi S,Hoppmann S,Xu Y,Cheng Z

    更新日期:2019-10-01 00:00:00

  • Development of antibody-carrying microbubbles based on clinically available ultrasound contrast agent for targeted molecular imaging: a preliminary chemical study.

    abstract:PURPOSE:The purpose of the study was to examine the feasibility of an antibody-carrying targeted-bubble preparation using clinically available phosphatidylserine (PS)-containing perfluorobutane-filled microbubbles for molecular ultrasound imaging. PROCEDURES:Firstly, we examined whether PS on the surface of perfluorob...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Otani K,Yamahara K

    更新日期:2011-04-01 00:00:00

  • Indirect monitoring of endogenous gene expression by positron emission tomography (PET) imaging of reporter gene expression in transgenic mice.

    abstract:PURPOSE:Repetitive imaging with microPET of endogenous albumin gene expression by using transgenic mice in which the Herpes Simplex Virus Type 1 thymidine kinase (HSV1-tk) reporter gene is driven by the albumin promoter (AL-HSV1-tk). METHODS:Transgenic mice were imaged repeatedly on a microPET scanner with approximate...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Green LA,Yap CS,Nguyen K,Barrio JR,Namavari M,Satyamurthy N,Phelps ME,Sandgren EP,Herschman HR,Gambhir SS

    更新日期:2002-01-01 00:00:00

  • Detection of the prodrug-activating enzyme carboxypeptidase G2 activity with chemical exchange saturation transfer magnetic resonance.

    abstract:PURPOSE:The purpose of this study is to evaluate if the differential exchange rates with bulk water between amine and amide protons can be exploited using chemical exchange saturation transfer magnetic resonance (CEST-MR) to monitor the release of glutamate induced by carboxypeptidase G2 (CPG2), an enzyme utilized in c...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Jamin Y,Eykyn TR,Poon E,Springer CJ,Robinson SP

    更新日期:2014-04-01 00:00:00

  • Validation of fluorescence molecular tomography/micro-CT multimodal imaging in vivo in rats.

    abstract:PURPOSE:Rats are important preclinical models for studying breast cancer metastasis and bone pathologies. In these research areas, fluorescence molecular tomography (FMT) is commonly applied for quantitative three-dimensional (3D) imaging in mice. However, uncertainties due to strong depth dependency of FMT signal and ...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Vonwil D,Christensen J,Fischer S,Ronneberger O,Shastri VP

    更新日期:2014-06-01 00:00:00

  • Mapping pH at Cancer Cell Surfaces.

    abstract:PURPOSE:To develop a tool to measure the pH at the surfaces of individual cells. PROCEDURES:The SNARF pH-sensitive dye was conjugated to a pHLIP® peptide (pH-Low Insertion Peptide) that binds cellular membranes in tumor spheroids. A beam splitter allows simultaneous recording of two images (580 and 640 nm) by a CCD ca...

    journal_title:Molecular imaging and biology

    pub_type: 杂志文章


    authors: Wei D,Engelman DM,Reshetnyak YK,Andreev OA

    更新日期:2019-12-01 00:00:00

  • Biodistribution and Radiation Dosimetry of [18F]Mefway in Humans.

    abstract:PURPOSE:[18F]Mefway is a positron emission tomography (PET) radioligand for quantification of the brain serotonin 1A (5-HT1A) receptor density. The purpose of this study was to evaluate the radiation safety of [18F]Mefway in humans. PROCEDURES:Six healthy volunteers (three males and three females) were whole-body PET ...

    journal_title:Molecular imaging and biology

    pub_type: 临床试验,杂志文章


    authors: Choi JY,Lyoo CH,Kim JS,Kim KM,Lee M,Ryu YH

    更新日期:2016-12-01 00:00:00