Abstract：
PURPOSE:For targeted imaging and therapy of hepatocellular carcinoma (HCC), we established a chimeric promoter (EIIAPA) containing alpha-fetoprotein (AFP) promoter and hepatitis B virus enhancer II (EIIA) to control downstream expression of reporter and therapeutic genes. PROCEDURES:We combined AFP promoter and EIIA to establish a chimeric EIIAPA promoter, then developed a bi-cistronic plasmid vector containing HSV1-tk and luciferase genes controlled by EIIAPA to stably transfect HCC cells. The selective transcriptional activity of EIIAPA was assayed by bioluminescence imaging (BLI) and the function of EIIAPA was determined by in vivo microPET and BLI. RESULTS:The luciferase expression driven by EIIAPA was higher than that driven by AFP promoter in HCC cell lines. EIIAPA-tk induced cytotoxicity was observed only in HepG2 cells. Accumulation of ¹³¹I-FIAU and bioluminescent signal were detected on HepG2 tumors but not in parental tumors. The HepG2 tumors derived from lentiviral-transduced EIIAPA-tk expressing cells accumulated ¹²⁴I-FIAU whereas the ARO tumors did not. The transfected HepG2 tumors expressed adequate EIIAPA-controlled HSV1-TK and the tumor regressed after ganciclovir treatment. CONCLUSION:The chimeric EIIAPA is a potential candidate promoter for targeted imaging and gene therapy of HCC.
journal_name
Mol Imaging Bioljournal_title
Molecular imaging and biologyauthors
Hsieh YJ,Chen FD,Ke CC,Wang HE,Huang CJ,Hou MF,Lin KP,Gelovani JG,Liu RSdoi
10.1007/s11307-011-0509-zsubject
Has Abstractpub_date
2012-08-01 00:00:00pages
452-61issue
4eissn
1536-1632issn
1860-2002journal_volume
14pub_type
杂志文章abstract：PURPOSE:The aim of this study was to investigate the longitudinal positron emission tomography (PET) metabolic changes in the elderly. PROCEDURES:Nineteen nondemented subjects (mean Mini-Mental Status Examination 29.4 +/- 0.7 SD) underwent two detailed neuropsychological evaluations and resting 2-deoxy-2-[F-18]fluoro-...
journal_title：Molecular imaging and biology
pub_type： 临床试验,杂志文章
doi：10.1007/s11307-009-0247-7
更新日期：2010-04-01 00:00:00
abstract：PURPOSE:This study aims to determine a lumped constant (LC) value that can be applied to the 2-deoxy-2[18F]fluoro-D-glucose positron emission tomography (FDG-PET) study to yield a physiological value of cerebral metabolic rate of glucose (CMR(glc)) in normal brain. PROCEDURES:We developed a more robust method for dete...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1016/s1536-1632(02)00122-1
更新日期：2003-01-01 00:00:00
abstract：PURPOSE:Repetitive imaging with microPET of endogenous albumin gene expression by using transgenic mice in which the Herpes Simplex Virus Type 1 thymidine kinase (HSV1-tk) reporter gene is driven by the albumin promoter (AL-HSV1-tk). METHODS:Transgenic mice were imaged repeatedly on a microPET scanner with approximate...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1016/s1095-0397(01)00071-1
更新日期：2002-01-01 00:00:00
abstract：PURPOSE:Human pluripotent stem cell (hPSC)-derived dopaminergic neuron progenitor cells (DAPCs) are a potential therapy for Parkinson's disease (PD). However, their intracranial administration raises safety concerns including uncontrolled proliferation, migration and inflammation. Here, we apply a bimodal imaging appro...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-020-01499-4
更新日期：2020-10-01 00:00:00
abstract：PURPOSE:The aim of this study is to generate a four-class magnetic resonance imaging (MRI)-based attenuation map (μ-map) for attenuation correction of positron emission tomography (PET) data of the head area using a novel combination of short echo time (STE)/Dixon-MRI and a dedicated image segmentation method. PROCEDU...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-015-0849-1
更新日期：2015-12-01 00:00:00
abstract：PURPOSE:The mTOR kinase inhibitor AZD8055 inhibits both mTORC1 and mTORC2 leading to disruption of glucose metabolism and proliferation pathways. This study assessed the impact of single and multiple doses of AZD8055 on the uptake of the glucose metabolism marker 2-deoxy-2-[(18) F]fluoro-D-glucose ([(18) F]FDG) and the...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-013-0705-0
更新日期：2014-06-01 00:00:00
abstract：PURPOSE:This study aims to explore whether 4-(2S,4R)-[18F]fluoroglutamine (4-[18F]FGln) positron emission tomography (PET) imaging is helpful in identifying and monitoring MYCN-amplified neuroblastoma by enhanced glutamine metabolism. PROCEDURES:Cell uptake studies and dynamic small-animal PET studies of 4-[18F]FGln a...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-019-01330-9
更新日期：2019-12-01 00:00:00
abstract：PURPOSE:The purpose of this paper is to validate a rapid and cost-effective ex vivo technique, microCT-based virtual histology, as an alternative to MRI imaging for assessing the therapeutic response in genetically engineered mouse models of cancer. PROCEDURES:All animal procedures were conducted in accordance with th...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-010-0372-3
更新日期：2011-06-01 00:00:00
abstract：PURPOSE:[18F]Mefway is a positron emission tomography (PET) radioligand for quantification of the brain serotonin 1A (5-HT1A) receptor density. The purpose of this study was to evaluate the radiation safety of [18F]Mefway in humans. PROCEDURES:Six healthy volunteers (three males and three females) were whole-body PET ...
journal_title：Molecular imaging and biology
pub_type： 临床试验,杂志文章
doi：10.1007/s11307-016-0955-8
更新日期：2016-12-01 00:00:00
abstract：:Incidental adrenal lesions found on anatomic imaging are not uncommon. 2-Deoxy-2-[18F]fluoro-D-glucose positron emission tomography (FDG-PET) imaging is highly accurate in the differentiation of benign from malignant adrenal lesions, both in patients with proven malignancy and with adrenal lesions detected incidentall...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1016/s1536-1632(02)00018-5
更新日期：2002-10-01 00:00:00
abstract：:Erdheim-Chester disease (ECD) is a disseminated xanthogranulomatous infiltrative disease of unknown etiology due to infiltration of different organs and bones by foamy histiocytes. A 37-year-old male with cerebral and periorbital lesions was diagnosed with this rare disease and was evaluated with magnetic resonance im...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1016/j.mibio.2004.01.023
更新日期：2004-01-01 00:00:00
abstract：PURPOSE:The demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore, we evaluated the r...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-015-0890-0
更新日期：2016-04-01 00:00:00
abstract：PURPOSE:Inflammation plays a key role in the progression of intervertebral disk (IVD) herniation and associated low back pain. However, real-time spatial diagnosis of inflammation associated with acute disk herniation has not been investigated. We sought to detect local neutrophil and macrophage infiltration near disk ...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-016-1038-6
更新日期：2017-10-01 00:00:00
abstract：PURPOSE:Macrophage accumulation characterizes the development of atherosclerotic plaques, and the presence of certain macrophage subsets might be an indicator of plaque phenotype and (in)stability. The macrophage mannose receptor (MMR) is expressed on alternatively activated macrophages and found at sites of intraplaqu...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-017-1117-3
更新日期：2018-04-01 00:00:00
abstract：PURPOSE:Many neurological diseases affect small structures in the brain and, as such, reliable visual evaluation and accurate quantification are required. Recent technological developments made the clinical use of hybrid positron emission tomography/magnetic resonance (PET/MR) systems possible, providing both functiona...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-015-0824-x
更新日期：2015-10-01 00:00:00
abstract：PURPOSE:The aim of this work is to investigate the impact of tissue classification in magnetic resonance imaging (MRI)-guided positron emission tomography (PET) attenuation correction (AC) for whole-body (WB) Patlak net uptake rate constant (Ki) imaging in PET/MRI studies. PROCEDURES:WB dynamic PET/CT data were acquir...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-019-01338-1
更新日期：2019-12-01 00:00:00
abstract：PURPOSE:Ga-68-labeled prostate-specific membrane antigen (PSMA) ligands have been used clinically for positron emission tomography (PET) imaging of prostate cancer. However, F-18-labeled compounds offer several advantages, including the potential for delayed imaging, high starting activities enabling multidose preparat...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-015-0844-6
更新日期：2015-12-01 00:00:00
abstract：PURPOSE:The purpose of this study is to evaluate if the differential exchange rates with bulk water between amine and amide protons can be exploited using chemical exchange saturation transfer magnetic resonance (CEST-MR) to monitor the release of glutamate induced by carboxypeptidase G2 (CPG2), an enzyme utilized in c...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-013-0680-5
更新日期：2014-04-01 00:00:00
abstract：PURPOSE:This study was undertaken to determine if artifacts from misalignment of cardiac emission to transmission data is present in adenosine stress studies and if the artifact could be reproduced by intentional misalignment in normal exams. PROCEDURES:Seventy consecutive 82Rb myocardial perfusion studies were review...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-008-0139-2
更新日期：2008-07-01 00:00:00
abstract：PURPOSE:Prostate-specific membrane antigen (PSMA) continues to be the hallmark biomarker for prostate cancer as it is expressed on nearly all prostatic tumors. In addition, increased PSMA expression correlates with castration resistance and progression to the metastatic stage of the disease. Recently, we combined both ...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-019-01404-8
更新日期：2020-04-01 00:00:00
abstract：PURPOSE:Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was utilized in postmortem imaging of gadolinium (Gd) spatial distribution in a mouse tumor model postadministration of PEGylated Gd liposomal nanoparticles. PROCEDURES:PEGylated liposomal nanoparticles were formulated using a paramagnetic...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-009-0282-4
更新日期：2010-08-01 00:00:00
abstract：:The article "Pre-therapy Somatostatin Receptor-Based Heterogeneity Predicts Overall Survival in Pancreatic Neuroendocrine Tumor Patients Undergoing Peptide Receptor Radionuclide Therapy," was originally published electronically on the publisher's internet portal without open access. ...
journal_title：Molecular imaging and biology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s11307-018-1261-4
更新日期：2018-12-01 00:00:00
abstract：PURPOSE:Increased expression of the αvβ6 integrin correlates with advanced tumor grade and poor clinical outcome, identifying αvβ6 as a prognostic indicator and an attractive target for molecular imaging. This work investigated the ability of a disulfide-stabilized [64Cu]NOTA-αvβ6 cys-diabody to image αvβ6 expression i...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-017-1097-3
更新日期：2018-02-01 00:00:00
abstract：PURPOSE:We developed a bimolecular fluorescence complementation (BiFC) strategy using Dronpa, a new fluorescent protein with reversible photoswitching activity and fast responsibility to light, to monitor protein-protein interactions in cells. PROCEDURES:Dronpa was split at residue Glu164 in order to generate two Dron...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-010-0312-2
更新日期：2010-10-01 00:00:00
abstract：PURPOSE:The main purpose of this study was to understand how the positron emission tomography (PET) measure of the synaptic vesicle 2A (SV2A) protein varies in vivo during the development of temporal lobe epilepsy (TLE) in the kainic acid rat model. PROCEDURES:Twenty Sprague Dawley male rats were administered with mul...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-020-01488-7
更新日期：2020-10-01 00:00:00
abstract：PURPOSE:High-dose (HD) cytosine arabinoside (ara-C) is a major treatment in acute myeloblastic leukemia (AML) that can lead to cerebellar complications, although electroencephalogram, computed tomography, and magnetic resonance imaging remain normal. We conducted a prospective study to evaluate brain perfusion with sin...
journal_title：Molecular imaging and biology
pub_type： 临床试验,杂志文章
doi：10.1007/s11307-010-0409-7
更新日期：2011-08-01 00:00:00
abstract：PURPOSE:Using three publicly available small-animal atlases (Sprague-Dawley rat, MOBY, and Digimouse), we built three articulated atlases and present several applications in the scope of molecular imaging. PROCEDURES:Major bones/bone groups were manually segmented for each atlas skeleton. Then, a kinematic model for e...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-010-0386-x
更新日期：2011-10-01 00:00:00
abstract：PURPOSE:To develop a tool to measure the pH at the surfaces of individual cells. PROCEDURES:The SNARF pH-sensitive dye was conjugated to a pHLIP® peptide (pH-Low Insertion Peptide) that binds cellular membranes in tumor spheroids. A beam splitter allows simultaneous recording of two images (580 and 640 nm) by a CCD ca...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-019-01335-4
更新日期：2019-12-01 00:00:00
abstract：PURPOSE:The sigma-2 (σ(2)) receptor is a potential biomarker of proliferative status of solid tumors. Specific synthetic probes using N-substituted-9-azabicyclo [3.3.1]nonan-3α-yl carbamate analogs have been designed and implemented for experimental cancer diagnosis and therapy. PROCEDURES:We employed the fluorescentl...
journal_title：Molecular imaging and biology
pub_type： 杂志文章
doi：10.1007/s11307-011-0493-3
更新日期：2012-06-01 00:00:00
abstract：PURPOSE:To evaluate bone blood flow and bone formation in patients after total hip revision surgery with impacted bone grafting using H2 15O and [18F]fluoride positron emission tomography (PET). PROCEDURES:To asses bone blood flow and bone metabolism in bone allograft after impaction grafting, four patients treated wi...
journal_title：Molecular imaging and biology
pub_type： 临床试验,杂志文章
doi：10.1007/s11307-008-0153-4
更新日期：2008-09-01 00:00:00
