Abstract:
:B-cell lymphoma remains one of the most refractory tumors, and as such the development of novel treatment approaches, such as antibody-drug conjugates (ADCs), is required. To improve the stability and homogeneity of the ADCs, a humanized anti-CD19 monoclonal antibody (RC58) was developed in the present study. RC58 was based on the CD19 antigen as a potential molecular target of human B-cell lymphomas. RC58 has high CD19-binding affinity and can be internalized in CD19-positive cells through endocytosis. Furthermore, three types of RC58-based ADCs (ADC-1, ADC-2, and ADC-3) were generated using three kinds of Maleimide-PEG-based linkers with two different cytotoxins. The anti-tumor activities of the ADCs were confirmed by in vitro and in vivo experiments. The stability of the ADCs was also evaluated by incubation in human plasma for 10 days. In vitro experiments showed that the three ADCs had distinct inhibitory effects on several B-lymphoma cell lines. Meanwhile, a close correlation between efficacy and drug concentration was found in a nude mouse xenograft model of human B-cell lymphoma, after treatment with RC58-based ADCs. Our results suggest that ADC-1, with high efficiency, could be used as a potential therapeutic agent for human B-cell malignancies.
journal_name
Int Immunopharmacoljournal_title
International immunopharmacologyauthors
Li Z,Wang M,Yao X,Li H,Li S,Liu L,Yu D,Li X,Fang J,Huang Cdoi
10.1016/j.intimp.2018.06.034subject
Has Abstractpub_date
2018-09-01 00:00:00pages
299-308eissn
1567-5769issn
1878-1705pii
S1567-5769(18)30285-6journal_volume
62pub_type
杂志文章abstract:BACKGROUND AND AIMS:In the past decade of clinical studies, the combination of chemotherapy with cytokine induced killer (CIK) cell transfusion has confirmed a promised efficacy in several types of cancer. CIK cells are a mixture of T lymphocytes, generated from peripheral blood mononuclear cells induced by multiple cy...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2016.12.005
更新日期:2017-05-01 00:00:00
abstract::Flavonoids, particularly those derived from plants, harbor biological effects such as anti-inflammation and the inhibition of cancer progression. In the present study, we investigated the effects of 10 kinds of flavonoids isolated from Nepalese propolis on the LPS signaling pathway in order to clarify their anti-infla...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2016.10.008
更新日期:2016-11-01 00:00:00
abstract::Apoptotic pathways play an important role in Mycobacterium tuberculosis-infected macrophages. Sirt1 is a member of the deacetylase family that is known to promote apoptosis resistance in many mammalian cells. However, the apoptotic role of Sirt1 in the process of M. tuberculosis infection remains unclear. With the hel...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.107283
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:Allergic rhinitis (AR) is characterized by a Th2 polarized immune response and soluble HLA (sHLA) molecules play an immunomodulatory role in this response. Previously, it has been reported that these molecules are increased in sera of patients with pollen-induced allergic rhinitis studied outside the pollen ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2009.04.014
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND:Although it is widely accepted that the "hygiene hypothesis" explains the increased incidence of asthma, the lack of suitable animal models hinders further in-depth studies of the underlying molecular immune mechanisms. Therefore, we aimed to develop a robust mouse asthma model to investigate the role of bac...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106453
更新日期:2020-06-01 00:00:00
abstract::We investigated the anti-inflammatory effects of sauchinone, a lignan isolated from Saururus chinensis, and the underlying mechanism in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. To assess the effects of sauchinone on LPS-induced macrophages activation, we measured the production of tumor necrosis factor (TNF...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2010.06.001
更新日期:2010-09-01 00:00:00
abstract::Caffeic acid phenethyl ester (CAPE), an the active component of propolis, is known to have anticarcinogenic, antiviral and various biological activities; however, the effect of CAPE on the immunomodulatory activity in vivo remains unknown. We have investigated the effect of CAPE on the immune system in female Balb/c m...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2004.01.013
更新日期:2004-03-01 00:00:00
abstract::Antrodia camphorata (AC) is a commonly used fungus in folk medicine for the treatment of viral hepatitis and cancer. AC polysaccharides (AC-PS) are reported to possess anti-inflammatory, anti-hepatitis B virus, and anticancer activities. In this study, we tested the in vivo effect of AC-PS on immune function by evalua...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2007.11.008
更新日期:2008-03-01 00:00:00
abstract::Multiple sclerosis (MS) is a chronic inflammatory demyelinating disorder in which lymphocytic infiltration mediated mainly by pro-inflammatory cytokines. In this study, we examined the effect of combined exercise training on the levels of IFN-γ, IL-4 and IL-17 in the plasma and the supernatant of peripheral blood lymp...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2010.08.008
更新日期:2010-11-01 00:00:00
abstract::High mobility group box protein 1 (HMGB1) and receptor for the advanced glycation end product (RAGE) play important roles in the development of sepsis-induced acute lung injury (ALI). Ketamine is considered to confer protective effects on ALI during sepsis. In this study, we investigated the effects of ketamine on HMG...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2016.01.021
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:It has been demonstrated that serum leptin is elevated in females with allergic rhinitis. Recently, it has been reported that one course of sublingual immunotherapy (SLIT) does not affect serum leptin levels. OBJECTIVE:The aim of this study was to evaluate the serum leptin levels in a cohort of patients wit...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2009.05.007
更新日期:2009-09-01 00:00:00
abstract::Inflammation is fundamental in osteoarthritis (OA) pathogenesis. Semaphorin 4A (Sema4A) has been implicated in immune-associated diseases, however, its role in OA remains unclear. In this study, we show that Sema4A is upregulated in knee OA articular cartilage as well as in chondrocytes exposed to IL-1β treatment in v...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.01.006
更新日期:2019-04-01 00:00:00
abstract::Natural killer (NK) cells eliminate cancer cells in a contact-dependent manner. However, how NK cells find cancer cells remain unclear. Here, using time-lapse imaging, we investigated how individual NK cells migrate toward cancer cells. Although naïve B16F10 cancer cells produce low levels of chemokines, IFN-γ-treated...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2018.07.026
更新日期:2018-10-01 00:00:00
abstract::IL-10 producing regulatory type 1 (Tr1) cells represents a subpopulation of CD4+ regulatory cells able to prevent in vitro bystander T-cell proliferation and to inhibit a wide range of inflammatory diseases in mice. Our aim was to evaluate the frequency and function of joint specific Tr1 cells in the peripheral blood ...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2011.03.001
更新日期:2011-08-01 00:00:00
abstract::Endometriosis is a gynecologic disorder characterized by the ectopic growth of misplaced endometrial cells. Moreover, immunological abnormalities of cell-mediated and humoral immunity may be associated with the pathogenesis of endometriosis. The effects of peritoneal fluid (PF) from endometriosis patients on the expre...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2005.05.004
更新日期:2005-11-01 00:00:00
abstract::High amounts of nitric oxide (NO) production following the induction of inducible NO synthase (iNOS) gene expression has been implicated in the pathogenesis of inflammatory diseases. Capsaicin, a vanilloid receptor agonist, is known to have an inhibitory effect on NO production in macrophages. In the present study, we...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/s1567-5769(01)00012-1
更新日期:2001-04-01 00:00:00
abstract::The changes in molecular structure and anti-inflammatory action of a gamma-irradiated quercetin were examined. Quercetin was gamma-irradiated at doses of 0, 15, 30, 50, 100 and 150kGy, which induced new radiolytic peaks (the highest radiolytic peak at a dose of 30kGy). Treatment of intact- and gamma-irradiated quercet...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2016.11.030
更新日期:2017-01-01 00:00:00
abstract::This study aims to explore the protective effects and mechanisms of ulinastatin (UTI), which is a urinary trypsin inhibitor, of the renal interstitial fibrosis of rats subjected to unilateral ureteral obstruction (UUO). A total of 36 male Wistar rats were divided in random into three groups, namely, the sham operation...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2012.12.019
更新日期:2013-02-01 00:00:00
abstract::Chelidonic acid (CA) is known as an inhibitor of the rat brain glutamate decarboxylase. However, the pharmacological effects of CA in allergic reactions have not yet been defined. Here, we show the effects and the mechanism of CA in the ovalbumin (OVA)-sensitized allergic rhinitis (AR) model. CA significantly decrease...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2010.10.002
更新日期:2011-01-01 00:00:00
abstract::CXCR4 is a member of chemokine receptors and plays a vital role in numerous diseases and cancer processes, which makes the CXCR4/CXCL12 chemotactic axis a potential therapeutic target. In this study, we used lentiviral vectors as a novel technology to produce a monoclonal antibody against CXCR4. Lentivirus vector pLV-...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2016.04.020
更新日期:2016-07-01 00:00:00
abstract::Acetylcholine (ACh) acts as a local cellular signaling molecule and is widely expressed in nature, including mammalian cells and embryonic stem cells. The murine embryonic stem cell line CGR8 synthesizes and releases substantial amounts of ACh. Particularly during early differentiation - a period associated with multi...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2015.04.005
更新日期:2015-11-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a high morbidity and disability disease with numerous inflammatory cells infiltrating in interstitial of articular cartilages and bones. As the most abundant inflammatory cells, neutrophil has been reported that their apoptosis changed gradually in the circumstance of RA. Apoptosis, one mo...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2018.09.011
更新日期:2018-12-01 00:00:00
abstract::Pseudoginsenoside-F11 (PF11), an ocotillol-type saponin, has neuroprotective effects on permanent and transient cerebral ischemia in rats by alleviating autophagic/lysosomal defects and repressing calcium overload, respectively. Ischemic stroke triggers peripheral innate immune cells, mainly neutrophils and macrophage...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2020.106564
更新日期:2020-08-01 00:00:00
abstract::Recent studies on the immunosuppressive effects of ultraviolet radiation (UVR) and the related resistance to infections in rodents and humans are presented. The waveband dependency of trans-to-cis isomerisation of urocanic acid in the stratum corneum and the role of DNA damage in UVR-induced erythema and immunosuppres...
journal_title:International immunopharmacology
pub_type: 杂志文章,评审
doi:10.1016/s1567-5769(01)00178-3
更新日期:2002-02-01 00:00:00
abstract::The aim of this study was to evaluate possible association between ophthalmic complications in sulfur mustard (SM) exposed patients with mild ocular injuries and serum soluble adhesion molecules. Serum levels of sICAM-1, sL-selectin, sP-selectin and sE-selectin in 367 SM-exposed individuals with or without eye injurie...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2012.12.014
更新日期:2013-11-01 00:00:00
abstract::The protective effects of genipin against lipopolysaccharide (LPS)-induced acute lung injury (ALI) have been reported; however, the mechanism is unclear. Genipin performs its pharmacological effects via activation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway. In...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105842
更新日期:2019-11-01 00:00:00
abstract::Cisplatin is a widely used antineoplastic drug, but its clinical usefulness is limited due to dose dependent nephrotoxicity. Nordihydroguaiaretic acid (NDGA) is a natural compound with broad pharmacological properties like antioxidant, anti-inflammatory and anticancer activity. The present study was undertaken to eval...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2015.07.016
更新日期:2015-09-01 00:00:00
abstract::The accumulation of cytosolic dsDNA plays important roles in the regulation of cellular processes. However, whether cytosolic dsDNA is involved in the pathogenesis of rheumatoid arthritis (RA) is not clear. Therefore, the present study investigated the roles of cytosolic dsDNA in the modulation of inflammatory respons...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.105791
更新日期:2019-11-01 00:00:00
abstract::During the evolution of neoplastic diseases, dendritic cell (DC) functions are usually attenuated, and this presents a problem to DC-based immunotherapies against cancer. Here, we investigated the effects of angelan, an acidic polysaccharide isolated from Angelica gigas Nakai, on DC maturation. Angelan efficiently inc...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2006.08.017
更新日期:2007-01-01 00:00:00
abstract::Astilbin is a potential agent for autoimmune and inflammatory diseases and has a protective effect in mice with DSS-induced colitis. NK1.1- CD4+ NKG2D+ T cells are a subpopulation of regulatory T cells that produce TGF-β1 and IL-10. Whether astilbin directly promotes the induction of NK1.1- CD4+ NKG2D+ T cells and whe...
journal_title:International immunopharmacology
pub_type: 杂志文章
doi:10.1016/j.intimp.2019.106143
更新日期:2020-04-01 00:00:00