Abstract：
:Gram-negative bacteria possess a complex cell envelope that consists of a plasma membrane, a peptidoglycan cell wall and an outer membrane. The envelope is a selective chemical barrier1 that defines cell shape2 and allows the cell to sustain large mechanical loads such as turgor pressure3. It is widely believed that the covalently cross-linked cell wall underpins the mechanical properties of the envelope4,5. Here we show that the stiffness and strength of Escherichia coli cells are largely due to the outer membrane. Compromising the outer membrane, either chemically or genetically, greatly increased deformation of the cell envelope in response to stretching, bending and indentation forces, and induced increased levels of cell lysis upon mechanical perturbation and during L-form proliferation. Both lipopolysaccharides and proteins contributed to the stiffness of the outer membrane. These findings overturn the prevailing dogma that the cell wall is the dominant mechanical element within Gram-negative bacteria, instead demonstrating that the outer membrane can be stiffer than the cell wall, and that mechanical loads are often balanced between these structures.
journal_name
Naturejournal_title
Natureauthors
Rojas ER,Billings G,Odermatt PD,Auer GK,Zhu L,Miguel A,Chang F,Weibel DB,Theriot JA,Huang KCdoi
10.1038/s41586-018-0344-3subject
Has Abstractpub_date
2018-07-01 00:00:00pages
617-621issue
7715eissn
0028-0836issn
1476-4687pii
10.1038/s41586-018-0344-3journal_volume
559pub_type
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