Abstract:
:In lung cancer a deregulation of Transforming Growth Factor-β (TGFβ) signaling has been observed. Yet, the impact of TGFβ in squamous cell carcinoma of the lung (LUSC) remained to be determined. We combined phenotypic and transcriptome-wide studies and showed that the stimulation of the LUSC cell line SK-MES1 with TGFβ results in an increase of migratory invasive properties. The analysis of the dynamics of gene expression by next-generation sequencing revealed that TGFβ stimulation orchestrates the upregulation of numerous motility- and actin cytoskeleton-related genes. Among these the non-muscle myosin 10 (MYO10) showed the highest upregulation in a LUSC patient cohort of the Cancer Genome Atlas (TCGA). Knockdown of MYO10 abrogated TGFβ-induced collagen gel invasion of SK-MES1 cells. The analysis of MYO10 mRNA expression in paired tissues of 151 LUSC patients with corresponding 80-month clinical follow-up data showed that the mRNA expression ratio of MYO10 in tumor and tumor-free tissue is prognostic for overall survival of LUSC patients and predictive for the response of these patients to adjuvant chemotherapy. Thus, MYO10 represents a new clinical biomarker for this aggressive disease and due to its role in cellular motility and invasion could serve as a potential molecular target for therapeutic interventions in patients with LUSC.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Dvornikov D,Schneider MA,Ohse S,Szczygieł M,Titkova I,Rosenblatt M,Muley T,Warth A,Herth FJ,Dienemann H,Thomas M,Timmer J,Schilling M,Busch H,Boerries M,Meister M,Klingmüller Udoi
10.1038/s41598-018-27912-1subject
Has Abstractpub_date
2018-06-22 00:00:00pages
9517issue
1issn
2045-2322pii
10.1038/s41598-018-27912-1journal_volume
8pub_type
杂志文章abstract::Identification of nodal metastasis and tumor extranodal extension (ENE) is crucial for head and neck cancer management, but currently only can be diagnosed via postoperative pathology. Pretreatment, radiographic identification of ENE, in particular, has proven extremely difficult for clinicians, but would be greatly i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32441-y
更新日期:2018-09-19 00:00:00
abstract::The construction of three-dimensional graphene aerogels (GAs) is of great importance owing to their outstanding properties for various applications. Up to now, the combination of ultralow weight and super mechanical strength for GA remains a great challenge. Here we demonstrate the fabrication of cellular GAs by a fac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25830
更新日期:2016-05-12 00:00:00
abstract::The intrinsic atomic mechanisms responsible for electronic doping of epitaxial graphene Moirés on transition metal surfaces is still an open issue. To better understand this process we have carried out a first-principles full characterization of the most representative Moiré superstructures observed on the Gr/Pt(111) ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20354
更新日期:2016-02-08 00:00:00
abstract::Phytoplankton subsumes the great variety of unicellular photoautotrophs that perform roughly half of Earth's primary production. They achieve this despite their challenging oceanic habitat, with opposing vertical gradients of nutrients (which often limit their growth near the surface) and light (which becomes limiting...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57890-2
更新日期:2020-01-24 00:00:00
abstract::Antiretroviral therapy containing an integrase strand transfer inhibitor (INSTI) plus two NRTIs has become the recommended treatment for antiretroviral-naive HIV-1-infected patients in the updated guidelines. We aimed to determine the prevalence of INSTI-related mutations in Taiwan. Genotypic resistance assays were pe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35779
更新日期:2016-10-25 00:00:00
abstract::In this study, we categorize the microbial community in mangrove sediment samples from four different locations within a vast mangrove system in Kerala, India. We compared this data to other samples taken from the other known mangrove data, a tropical rainforest, and ocean sediment. An examination of the microbial com...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09254-6
更新日期:2017-08-18 00:00:00
abstract::The morbidity and mortality rates of human immunodeficiency virus (HIV)-hepatitis B virus (HBV) coinfection are higher than that of either infection alone. Outcomes and the virological response to antiretrovirals (combination antiretroviral therapy, cART) were explored in HIV/HBV subjects in a cohort of Italian patien...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46976-1
更新日期:2019-08-16 00:00:00
abstract::Pulsatillae radix is a conventional traditional Chinese medicine (TCM) with common name Baitouweng, and has notable effects on inflammation and dysentery. Pulsatilla chinensis (Bge.) Regel is the only source plant of Baitouweng recorded in Chinese Pharmacopoeia, but its adulteration often occurs in the market that pos...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40000
更新日期:2017-01-06 00:00:00
abstract::Tropical Pacific variability (TPV) heavily influences global climate, but much is still unknown about its drivers. We examine the impact of South Pacific variability on the modes of TPV: the El Niño-Southern Oscillation (ENSO) and the Interdecadal Pacific Oscillation (IPO). We conduct idealised coupled experiments in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52805-2
更新日期:2019-12-04 00:00:00
abstract::Ultrasound has significantly increased safety and effectiveness in regional anesthesia. However, little is known about its clinical use. We studied clinical approaches currently used by anesthesiologists, conducted a nationwide survey, and analyzed data collected in ordered logistic regression models. All active membe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45700-3
更新日期:2019-06-24 00:00:00
abstract::Oscillatory media can exhibit the coexistence of synchronized and desynchronized regions, so-called chimera states, for uniform parameters and symmetrical coupling. In a phase-balanced chimera state, where the totals of synchronized and desynchronized regions, respectively, are of the same size, the symmetry of the sy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep09883
更新日期:2015-04-30 00:00:00
abstract::The combination of genome-edited human embryonic stem cells (hESCs) and subsequent neural differentiation is a powerful tool to study neurodevelopmental disorders. Since the naïve state of pluripotency has favourable characteristics for efficient genome-editing, we optimized a workflow for the CRISPR/Cas9 system in th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16932-y
更新日期:2017-11-30 00:00:00
abstract::WRINKLED1 (WRI1), an APETALA2 (AP2)-type transcription factor, has been shown to be required for the regulation of carbon partitioning into fatty acid (FA) synthesis in plant seeds. To our knowledge, the regulatory network of WRI1 remains unknown in Prunus sibirica kernel (PSK), a novel woody biodiesel feedstock in Ch...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39236-9
更新日期:2019-02-25 00:00:00
abstract::Pyramidal neurons in the rodent hippocampus exhibit spatial tuning during spatial navigation, and they are reactivated in specific temporal order during sharp-wave ripples observed in quiet wakefulness or slow wave sleep. However, analyzing representations of sleep-associated hippocampal ensemble spike activity remain...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32193
更新日期:2016-08-30 00:00:00
abstract::Multilevel severe compressive myelopathy is a challenging disorder for the surgeons, the aim of this study is to assess the efficacy and safety of a newly designed ultrasonic burr as an assistant tool to the ultrasonic scalpel in laminectomy for this disease. This is a retrospective comparative study, the included sub...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22454-y
更新日期:2018-03-05 00:00:00
abstract::Breast cancer survivors display altered inflammatory responses to immune challenges relative to cancer-naive controls likely due to previous cancer treatments, stress associated with cancer, and/or tumor physiology. Proper inflammatory responses are necessary for adaptive sickness behaviors (e.g., fatigue, anorexia, a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37334-8
更新日期:2019-01-24 00:00:00
abstract::The origin of the enantio- and regioselectivity of ring-opening reaction of oxabicyclic alkenes catalyzed by rhodium/Josiphos has been examined using M06-2X density functional theory(DFT). DFT calculations predict a 98% ee for the enantioselectivity and only the 1,2-trans product as one regio- and diastereomer, in exc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40491
更新日期:2017-01-11 00:00:00
abstract::We generated self-induced retinal ganglion cells (RGCs) with functional axons from human induced pluripotent stem cells. After development of the optic vesicle from the induced stem cell embryoid body in three-dimensional culture, conversion to two-dimensional culture, achieved by supplementation with BDNF, resulted i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08344
更新日期:2015-02-10 00:00:00
abstract::Reactive oxygen species (ROS) participate in various physiological and pathological functions following generation from different types of cells. Here we explore ROS functions on spontaneous tail regeneration using gecko model. ROS were mainly produced in the skeletal muscle after tail amputation, showing a temporal i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20752
更新日期:2016-02-08 00:00:00
abstract::Optical clearing methods can facilitate deep optical imaging in biological tissue by reducing light scattering and this has enabled accurate three-dimensional signal visualization and quantification of complex biological structures. Unfortunately, existing optical clearing approaches present a compromise between maxim...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12484-3
更新日期:2017-09-22 00:00:00
abstract::Hysteresis, which is the history dependence of physical systems, is one of the most important topics in physics. Interestingly, bi-stability of plasma with a huge hysteresis loop has been observed in inductive plasma discharges. Despite long plasma research, how this plasma hysteresis occurs remains an unresolved ques...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15254
更新日期:2015-10-20 00:00:00
abstract::HAUSP (herpes virus-associated ubiquitin specific protease, known as ubiquitin specific protease 7), one of DUBs, regulates the dynamics of the p53 and Mdm2 network in response to DNA damage by deubiquitinating both p53 and its E3 ubiquitin ligase, Mdm2. Its concerted action increases the level of functional p53 by pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12793
更新日期:2015-08-04 00:00:00
abstract::Zirconium compounds has been widely attention over the last decades due to its excellent physical and chemical properties. Zirconium nitride nanopowders were synthesized via a simple direct carbothermic nitridation process of internal gel derived zirconia in the presence of nano-sized carbon black. The effects of reac...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55450-x
更新日期:2019-12-16 00:00:00
abstract::The detection and characterization of highly enriched uranium (HEU) presents a large challenge in the non-proliferation field. HEU has a low neutron emission rate and most gamma rays are low energy and easily shielded. To address this challenge, an instrument known as the dual-particle imager (DPI) was used with a por...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-08253-x
更新日期:2017-08-11 00:00:00
abstract::High-throughput RNA sequencing is a powerful tool that allows us to perform gene prediction and analyze tissue-specific overexpression of genes, but also at species level comparisons can be performed, although in a more restricted manner. In the present study complete liver transcriptomes of five tropical bat species ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42560-9
更新日期:2019-04-17 00:00:00
abstract::Ganoderma lucidum polysaccharide peptide (GLPP) scavenges oxygen free radicals that are a key factor in the pathogenesis of renal ischemia reperfusion injury (RIRI). The aim of this study was to determine whether GLPP could attenuate RIRI by counteracting the oxidative stress. The mechanism involved was assessed by an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16910
更新日期:2015-11-25 00:00:00
abstract::Chronic airway infections caused by Pseudomonas aeruginosa contribute to the progression of pulmonary disease in individuals with cystic fibrosis (CF). In the setting of CF, within-patient adaptation of a P. aeruginosa strain generates phenotypic diversity that can complicate microbiological analysis of patient sample...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10932
更新日期:2015-06-05 00:00:00
abstract::Functional neuroimaging studies have implicated regions of both ventrolateral prefrontal cortex (VLPFC) and angular gyrus in processes associated with retrieving goal-relevant information, which increases the fidelity and richness of long-term memory (LTM). To further investigate the roles of these cortical regions as...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33164-w
更新日期:2018-10-05 00:00:00
abstract::Doubly curved nanofiber-reinforced optically transparent composites with low thermal expansion of 15 ppm/k are prepared by hot pressing vacuum-filtered Pickering emulsions of hydrophobic acrylic resin monomer, hydrophilic chitin nanofibers and water. The coalescence of acrylic monomer droplets in the emulsion is preve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16421
更新日期:2015-11-10 00:00:00
abstract::Research on argillite as a possible host rock for nuclear waste disposal is still an open subject since many issues need to be clarified. In the Underground Research Laboratories constructed for this purpose, a damaged zone around the excavation has been systematically observed and characterized by the appearance of m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08815
更新日期:2015-03-06 00:00:00