Abstract：
:Chronic airway infections caused by Pseudomonas aeruginosa contribute to the progression of pulmonary disease in individuals with cystic fibrosis (CF). In the setting of CF, within-patient adaptation of a P. aeruginosa strain generates phenotypic diversity that can complicate microbiological analysis of patient samples. We investigated within- and between- sample diversity of 34 phenotypes among 235 P. aeruginosa isolates cultured from sputum samples collected from a single CF patient over the span of one year, and assessed colony morphology as a screening tool for predicting phenotypes, including antimicrobial susceptibilities. We identified 15 distinct colony morphotypes that varied significantly in abundance both within and between sputum samples. Substantial within sample phenotypic heterogeneity was also noted in other phenotypes, with morphotypes being unreliable predictors of antimicrobial susceptibility and other phenotypes. Emergence of isolates with reduced susceptibility to β-lactams was observed during periods of clinical therapy with aztreonam. Our findings confirm that the P. aeruginosa population in chronic CF lung infections is highly dynamic, and that intra-sample phenotypic diversity is underestimated if only one or few colonies are analyzed per sample.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Clark ST,Diaz Caballero J,Cheang M,Coburn B,Wang PW,Donaldson SL,Zhang Y,Liu M,Keshavjee S,Yau YC,Waters VJ,Elizabeth Tullis D,Guttman DS,Hwang DMdoi
10.1038/srep10932subject
Has Abstractpub_date
2015-06-05 00:00:00pages
10932issn
2045-2322pii
srep10932journal_volume
5pub_type
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2015-10-07 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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