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Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing.

Abstract：

:Recent use of the CRISPR/Cas9 system has dramatically reduced the time required to produce mutant mice, but the involvement of a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed a simple, highly efficient, and large-scale genome editing method, in which the RNAs for the CRISPR/Cas9 system are electroporated into zygotes rather than microinjected. We used this method to perform single-stranded oligodeoxynucleotide (ssODN)-mediated knock-in in mouse embryos. This method facilitates large-scale genetic analysis in the mouse.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Hashimoto M,Takemoto T

doi

10.1038/srep11315

subject

Has Abstract

pub_date

2015-06-11 00:00:00

pages

11315

issn

2045-2322

pii

srep11315

journal_volume

5

pub_type

杂志文章

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