Abstract：
:Pigeon pea is an important legume infested by a plethora of insect pests amongst which gram pod borer Helicoverpa armigera is very prominent. Imparting resistance to this insect herbivore is of global importance in attaining food security. Expression of insecticidal crystal proteins (ICP) in diverse crops has led to increased resistance to several pests. We report in this paper, expression of Cry2Aa in transgenic pigeon pea and its effectiveness towards H. armigera by employing Agrobacterium-mediated in planta transformation approach. Approximately 0.8% of T1 generation plants were identified as putative transformants based on screening in the presence of 70 ppm kanamycin as the selection agent. Promising events were further recognized in advanced generations based on integration, expression and bioefficacy of the transgenes. Seven T3 lines (11.8% of the selected T1 events) were categorized as superior as these events demonstrated 80-100% mortality of the challenged larvae and improved ability to prevent damage caused by the larvae. The selected transgenic plants accumulated Cry2Aa in the range of 25-80 µg/g FW. The transgenic events developed in the study can be used in pigeon pea improvement programmes for pod borer resistance.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Singh S,Kumar NR,Maniraj R,Lakshmikanth R,Rao KYS,Muralimohan N,Arulprakash T,Karthik K,Shashibhushan NB,Vinutha T,Pattanayak D,Dash PK,Kumar PA,Sreevathsa Rdoi
10.1038/s41598-018-26358-9subject
Has Abstractpub_date
2018-06-11 00:00:00pages
8820issue
1issn
2045-2322pii
10.1038/s41598-018-26358-9journal_volume
8pub_type
杂志文章abstract：:The widespread use of electric light and electronic devices has resulted in an excessive exposure to light during the late-evening and at night. This late light exposure acutely suppresses melatonin and sleepiness and delays the circadian clock. Here we investigate whether the acute effects of late-evening light expos...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-019-52352-w
更新日期：2019-11-05 00:00:00
abstract：:Irreversible electroporation (IRE) is an emerging focal therapy which is demonstrating utility in the treatment of unresectable tumors where thermal ablation techniques are contraindicated. IRE uses ultra-short duration, high-intensity monopolar pulsed electric fields to permanently disrupt cell membranes within a wel...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep14999
更新日期：2015-10-13 00:00:00
abstract：:Subclinical hypothyroidism has been associated with dyslipidemia, hypertension, and increased risk of coronary artery disease. However, limited is known for its effect on renal function. Here we aimed to investigate whether subclinical hypothyroidism is associated with reduced estimated glomerular filtration rate (eGF...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19693-4
更新日期：2018-02-01 00:00:00
abstract：:Short-term prospective studies have suggested that pulmonary tuberculosis (TB) preludes permanent loss of lean tissue and fat mass even when TB treatment is effective. The aim of this study was to estimate the risk of sarcopenia and osteoporosis among Korean male TB survivors. Data of the population-based, Korea Natio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12419-y
更新日期：2017-10-13 00:00:00
abstract：:With the development of ultra-intense laser technology, MeV ions can be obtained from laser-foil interactions in the laboratory. These energetic ion beams can be applied in fast ignition for inertial confinement fusion, medical therapy, and proton imaging. However, these ions are mainly accelerated in the laser propag...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08274
更新日期：2015-02-05 00:00:00
abstract：:From the viewpoint of thermodynamics, the formation of DNA loops and the interaction between them, which are all non-equilibrium processes, result in the change of free energy, affecting gene expression and further cell-to-cell variability as observed experimentally. However, how these processes dissipate free energy ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12765-x
更新日期：2017-10-03 00:00:00
abstract：:A topical medication combining calcipotriol (Cal) and betamethasone dipropionate (BDP) has proven effective in a number of randomized controlled trials performed in patients with psoriasis, but its mechanism of action has not been fully elucidated. We investigated whether the combination of Cal and BDP (Cal/BDP) in th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-52892-1
更新日期：2019-11-08 00:00:00
abstract：:Changes in climate and atmospheric CO2 and nitrogen (N) over the last several decades have induced significant effects on forest carbon (C) cycling. However, contributions of individual factors are largely unknown because of the lack of long observational data and the undifferentiating between intrinsic factors and ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04461
更新日期：2014-03-25 00:00:00
abstract：:Spotted seal (Phoca largha) is a critically endangered pinniped in China and South Korea. The conventional method to protect and maintain the P. largha population is to keep them captive in artificially controlled environments. However, little is known about the physiological differences between wild and captive P. la...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75759-2
更新日期：2020-10-30 00:00:00
abstract：:Negative diagonal magneto-conductivity/resistivity is a spectacular- and thought provoking-property of driven, far-from-equilibrium, low dimensional electronic systems. The physical response of this exotic electronic state is not yet fully understood since it is rarely encountered in experiment. The microwave-radiatio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03478
更新日期：2013-12-11 00:00:00
abstract：:Stabilization exercise (SE) is evident for the management of chronic non-specific low back pain (LBP). The optimal dose-response-relationship for the utmost treatment success is, thus, still unknown. The purpose is to systematically review the dose-response-relationship of stabilisation exercises on pain and disabilit...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/s41598-020-73954-9
更新日期：2020-10-09 00:00:00
abstract：:Recreational boating is an increasing activity in coastal areas and its spatiotemporal overlap with key habitats of marine species pose a risk for negative noise impacts. Yet, recreational vessels are currently unaccounted for in vessel noise models using Automatic Identification System (AIS) data. Here we conduct a c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51222-9
更新日期：2019-10-29 00:00:00
abstract：:Spiral waves anchored to obstacles in cardiac tissues may cause lethal arrhythmia. To unpin these anchored spirals, comparing to high-voltage side-effect traditional therapies, wave emission from heterogeneities (WEH) induced by the uniform electric field (UEF) has provided a low-voltage alternative. Here we provide a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04831
更新日期：2014-04-29 00:00:00
abstract：:Pot experiments were carried out to study the effects of rice straw (RS) and rice straw ash (RSA) on the growth of early rice and α-diversity of bacterial community in soils around rare earth mining areas of Xunwu and Xinfeng counties in South Jiangxi of China. The results showed that the exploitation of rare earth re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67160-w
更新日期：2020-06-25 00:00:00
abstract：:Agrobacterium pathogens of octopine- and nopaline-types force host plants to produce either octopine or nopaline compounds, which they use as nutrients. Two Agrobacterium ABC-transporters and their cognate periplasmic binding proteins (PBPs) OccJ and NocT import octopine and nopaline/octopine, respectively. Here, we s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18243-8
更新日期：2017-12-21 00:00:00
abstract：:Charge noise is critical in the performance of gate-controlled quantum dots (QDs). Such information is not yet available for QDs made out of the new material graphene, where both substrate and edge states are known to have important effects. Here we show the 1/f noise for a microscopic graphene QD is substantially lar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08142
更新日期：2015-01-30 00:00:00
abstract：:To better understand the recognition mechanism of RISC and the repertoire of guide-target interactions we introduced G:U wobbles and mismatches at various positions of the microRNA (miRNA) 'seed' region and performed all-atom molecular dynamics simulations of the resulting Ago-miRNA:mRNA ternary complexes. Our simulat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00569
更新日期：2012-01-01 00:00:00
abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is a difficult-to-treat infection. Increasing efforts have been taken to mitigate the epidemics and to avoid potential outbreaks in low endemic settings. Understanding the population dynamics of MRSA is essential to identify the causal mechanisms driving the epidemics...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66270-9
更新日期：2020-06-09 00:00:00
abstract：:Thermo-elasticity couples the deformation of an elastic (solid) body to its temperature and vice-versa. It is a solid-like property. Highlighting such property in liquids is a paradigm shift: it requires long-range collective interactions that are not considered in current liquid descriptions. The present microthermal...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69404-1
更新日期：2020-08-07 00:00:00
abstract：:Deregulated IGF-1R-AKT signaling influences multiple nodes of cancer cell physiology and assists in migration, metastasis and acquirement of radio/chemoresistance. Enrichment of cancer stem cells (CSC) positively correlates with radio/chemoresistance development in various malignancies. It is unclear though, how IGF-1...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36612
更新日期：2016-11-07 00:00:00
abstract：:Exosomes are nano-sized endosome-derived small intraluminal vesicles, which are important facilitators of intercellular communication by transporting contents, such as protein, mRNA, and microRNAs, between neighboring cells, such as in the tumor microenvironment. The purpose of this study was to understand the mechani...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30110
更新日期：2016-07-22 00:00:00
abstract：:This study investigated the pathophysiological features of pachychoroid neovasculopathy (PNV) and neovascular age-related macular degeneration (nAMD) by analysing and comparing cytokine profiles in aqueous humour (AH) collected from 18 PNV, 18 nAMD and 11 control patients. Responses to intravitreal injection of aflibe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28484-w
更新日期：2018-07-12 00:00:00
abstract：:To determine whether any brain MR abnormalities, including enlarged perivascular spaces (EPVS), were associated with disease activity in systemic lupus erythematosus (SLE) as an inflammatory activity. One hundred and thirty SLE patients with normal MR findings were assessed. With regard to MRI abnormalities, patients ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12966-4
更新日期：2017-10-03 00:00:00
abstract：:In recent years, active targeting strategies by ligand modification have emerged to enhance tumor accumulation of NP, but their clinical application was strictly restricted due to the complex preparation procedures, poor stability and serious toxicity. An effective and clinical translational strategy is required to sa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27669
更新日期：2016-06-08 00:00:00
abstract：:Dyslipidemia in pregnancy are associated with risk of adverse outcomes. As an adverse pregnancy outcome, small-for-gestational-age has been extensively studied in Western countries. However, similar studies have rarely been conducted in Asian countries. Data were derived from 5695 pairs of non-diabetic mothers and neo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76845-1
更新日期：2020-11-16 00:00:00
abstract：:To meet the demand for detection of foreign genes in genetically modified (GM) sugarcane necessary for regulation of gene technology, an efficient method with high specificity and rapidity was developed for the cry1Ac gene, based on loop-mediated isothermal amplification (LAMP). A set of four primers was designed usin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04912
更新日期：2014-05-09 00:00:00
abstract：:To discuss the status of epidemiology of stroke in the Mongolian and Han population aged over 45 years and to understand the treatment and prevention of stroke. Data collected on stroke populations aged over 45 years in the six areas in Inner Mongolia were analyzed by using stratified multi-stage cluster sampling. The...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45710
更新日期：2017-04-04 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-43507-w
更新日期：2019-06-05 00:00:00
abstract：:Danggui Buxue Tang (DBT) is a classic Chinese herbal formula which consists of Astragali mongholici Radix and Angelica sinensis Radix (ASR). For chemical ingredients, HPLC were performed. Results showed compared with single herbs, DBT decoction could promote the dissolution of ingredients such as ferulic acid and caly...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45111
更新日期：2017-03-22 00:00:00
abstract：:Planktonic foraminifera (PF) life cycles are highly sensitive to marine conditions, which are evolving rapidly due to anthropogenic climate change. Even though PF shells in the sedimentary record serve as prominent proxies of past ocean conditions, very little is still known about their life cycles, particularly in ol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74342-z
更新日期：2020-10-15 00:00:00
