Rapid Screening and Characterization of Acetylcholinesterase Inhibitors from Yinhuang Oral Liquid Using Ultrafiltration-liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry.

Abstract:

Background:At present, approximately 17-25 million people in the world suffer from Alzheimer's disease (AD). The most efficacious and acceptable therapeutic drug clinically are the acetylcholinesterase inhibitors (AChEIs). Yinhuang oral liquid is a Chinese medicine preparation which contains AChEIs according to the literatures. However, no strategy has been presented for rapid screening and identification of AChEIs from Yinhuang oral liquid. Objective:To develop a method for rapid screening and identification of AChEIs from Yinhuang oral liquid using ultrafiltration-liquid chromatography-electrospray ionization tandem mass spectrometry (UF-LC-ESI-MS/MS). Materials and Methods:In this study, UF incubation conditions such as enzyme concentration, incubation time, and incubation temperature were optimized so as to get better screening results. The AChEIs from Yinhuang oral liquid were identified by high-performance liquid chromatography-ESI-MS and the improved Ellman method was used for the AChE inhibitory activity test in vitro. Results:The results showed that Yinhuang oral liquid can inhibit the activity of AChE. We screened and identified seven compounds with potential AChE inhibitory activity from Yinhuang oral liquid, which provided experimental basis for the treatment and prevention of AD. Conclusion:The current technique was used to directly screen the active ingredients with acetylcholinesterase inhibition from complex traditional Chinese medicine, which was simple, rapid, accurate, and suitable for high-throughput screening of AChEI from complex systems. SUMMARY:A UF-LC-ESI-MS/MS method for rapid screening and identification of AChEIs from Yinhuang oral liquid was developedSeven compounds were screened and identified with potential AChE inhibitory activity from Yinhuang oral liquidIt provided experimental basis of Yinhuang oral liquid for the treating and preventing AD. Abbreviations used: (AD): Alzheimer's disease; (UF-LC-ESI-MS/MS): ultrafiltration-liquid chromatography-electrospray ionization tandem mass spectrometry; (AChEIs): acetylcholinesterase inhibitors.

journal_name

Pharmacogn Mag

journal_title

Pharmacognosy magazine

authors

Zhang H,Guo Y,Meng L,Sun H,Yang Y,Gao Y,Sun J

doi

10.4103/pm.pm_166_17

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

248-252

issue

54

eissn

0973-1296

issn

0976-4062

pii

PM-14-248

journal_volume

14

pub_type

杂志文章
  • Optimization Extraction Process of Polysaccharides from Suillus granulatus and Their Antioxidant and Immunological Activities In vitro.

    abstract:BACKGROUND:Suillus granulatus is an edible and medicinal fungus in China. S. granulatus polysaccharide (SGP) was considered as the main bioactivity compounds in S. granulatus. Therefore, the extraction of SGP and their antioxidant activities were studied in this work. MATERIALS AND METHODS:Fruiting bodies of S. granul...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.182161

    authors: Zhou F,Yan S,Chen S,Gong L,Su T,Wang Z

    更新日期:2016-05-01 00:00:00

  • Species identification of Rhododendron (Ericaceae) using the chloroplast deoxyribonucleic acid PsbA-trnH genetic marker.

    abstract:BACKGROUND:Rhododendron is a group of famous landscape plants with high medicinal value. However, there is no simple or universal manner to discriminate the various species of this group. Deoxyribonucleic acid (DNA) barcoding technique is a new biological tool that can accurately and objectively identify species by usi...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.93311

    authors: Liu Y,Zhang L,Liu Z,Luo K,Chen S,Chen K

    更新日期:2012-01-01 00:00:00

  • 7-hydroxycalamenene Effects on Secreted Aspartic Proteases Activity and Biofilm Formation of Candida spp.

    abstract:BACKGROUND:The 7-hydroxycalamenenene-rich essential oil (EO) obtained from the leaves of Croton cajucara (red morphotype) have been described as active against bacteria, protozoa, and fungi species. In this work, we aimed to evaluate the effectiveness of 7-hydroxycalamenenene against Candida albicans and nonalbicans sp...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.176022

    authors: Azevedo MM,Almeida CA,Chaves FC,Rodrigues IA,Bizzo HR,Alviano CS,Alviano DS

    更新日期:2016-01-01 00:00:00

  • Pretreatment with Assafoetida exerts dose-dependent dual effects on rat hearts.

    abstract:CONTEXT:Although many studies displayed the favorable effects of Assafoetida, some of them reported that high doses of Assafoetida could lead to harmful effects. AIMS:In this study, the effect of pretreatment with Assafoetida investigated on ischemic-reperfusion injury in isolated rat heart model. MATERIALS AND METHO...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.131026

    authors: Esmailidehaj M,Kakoo M,Rezvani ME,Mosaddeghmehrjardi MH

    更新日期:2014-04-01 00:00:00

  • Antiradical and antidiabetic properties of standardized extract of Sunderban mangrove Rhizophora mucronata.

    abstract:BACKGROUND:Mangroves have the ability to grow where no other vascular plants survive. Rhizophora mucronata is a true mangrove and traditionally used to treat diabetes and its allied complications. OBJECTIVES:In the present study, we standardized the 80% methanolic standardized extract of R. mucronata leaves (RH) and f...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.153094

    authors: Sur TK,Hazra AK,Bhattacharyya D,Hazra A

    更新日期:2015-04-01 00:00:00

  • Determination of oleanolic acid and ursolic acid in Chinese medicinal plants using HPLC with PAH polymeric C18.

    abstract:BACKGROUND:The RP-HPLC resolution of two triterpenic acid isomers was unstable. OBJECTIVE:To separate the oleanolic acid (OA) and ursolic acid (UA) simply within RP-HPLC. MATERIALS AND METHODS:The separation ability of five stationary phases was studied with the retention effect of their carbon loads. Also the resolu...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.117853

    authors: Zhang Y,Xue K,Zhao EY,Li Y,Yao L,Yang X,Xie X

    更新日期:2013-10-01 00:00:00

  • Extraction of Chelerythrine and its Effects on Pathogenic Fungus Spore Germination.

    abstract:Background:Chemical fungicides are widely used to control crop diseases, but these chemicals have adverse effects. They destroy the ecological environment and even have toxic effects on human beings. In this context, the development of botanical pesticides is relevant. One potential botanical pesticide is chelerythrine...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_545_16

    authors: Wei Q,Zhao M,Li X

    更新日期:2017-10-01 00:00:00

  • Safety Profile Investigations of Meyna spinosa (Roxb.) and Oroxylum indicum (Linn.) Extracts Collected from Northeast India.

    abstract:Background:Meyna spinosa (M.S) (Roxb.) ex Link and Oroxylum indicum (O.I) (Linn.) Vent, widely used traditional Northeast Indian medicinal plant used for various purposes, have not yet explored for safety profile. Objective:To investigate the safety profile of M.S (Roxb.) ex Link leaves and O.I (Linn.) Vent stem bark ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_62_15

    authors: Singh S,Chattopadhyay P,Borthakur SK,Policegoudra R

    更新日期:2018-01-01 00:00:00

  • In vitro Anti-Proliferative Effect of Tephrosia purpurea on Human Hepatocellular Carcinoma Cells.

    abstract:BACKGROUND:Tephrosia purpurea is an Indian herb used in traditional medicine to treat various diseases such as jaundice, asthma, liver and urinary disorders. However, the anti-cancer potential of T. purpurea on hepatocellular carcinoma (HCC) is poorly understood. Therefore, this study aims to investigate the anti-cance...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.203981

    authors: Padmapriya R,Gayathri L,Ronsard L,Akbarsha MA,Raveendran R

    更新日期:2017-01-01 00:00:00

  • Evaluation of Hypolipidemic and Antioxidant Effects in Phenolrich Fraction of Crataegus pinnatifida Fruit in Hyperlipidemia Rats and Identification of Chemical Composition by Ultra-performance Liquid Chromatography Coupled with Quadropole Time-of-flight M

    abstract:Background:Hawthorn (Crataegus pinnatifida) fruit has enjoyed a great popularity as a pleasant-tasting food associated with hypolipidemic and antioxidant effects. Objective:Our aim was to screen the effective fraction of hawthorn fruit in the treatment of hyperlipidemia rats. Materials and Methods: In this study, etha...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_402_16

    authors: Shao F,Gu L,Chen H,Liu R,Huang H,Chen L,Yang M

    更新日期:2017-10-01 00:00:00

  • New ionic liquid-based preparative method for diosgenin from Rhizoma dioscoreae nipponicae.

    abstract:BACKGROUND:Rhizoma dioscoreae nipponicae is a perennial herb and its roots have been widely used in Traditional Chinese Medicine (TCM). OBJECTIVE:To develop and optimize the extraction and hydrolysis technology of diosgenin from Rhizoma dioscoreae nipponicae. MATERIALS AND METHODS:1-methyl-3-(3-sulfopropyl)-imidazoli...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.113282

    authors: Yan W,Ji L,Hang S,Shun Y

    更新日期:2013-07-01 00:00:00

  • Total Phenolics, Total Flavonoids, Antioxidant Capacities, and Volatile Compounds Gas Chromatography-Mass Spectrometry Profiling of Moringa oleifera Ripe Seed Polar Fractions.

    abstract:Background:Academic reports have confirmed Moringa oleifera leaves to possess significant antioxidant capacities; however, such studies are unavailable for its ripe seeds even though they are more desirous for consumption due to their sweet taste. Objective:In this study, we investigated antioxidant capacities of four...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_212_17

    authors: Adebayo IA,Arsad H,Samian MR

    更新日期:2018-04-01 00:00:00

  • Chemical constituents with free-radical-scavenging activities from the stem of Fissistigma polyanthum.

    abstract:BACKGROUND:Fissistigma polyanthum is a liane belonging to the Annonaceae family and it is one of the most important crude drugs in traditional Chinese medicine. OBJECTIVE:The objective was to describe the structural elucidation and the free-radical-scavenging activities of the isolated compounds from Fissistigma polya...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.96549

    authors: Fan H,Zheng T,Chen Y,Yang GZ

    更新日期:2012-04-01 00:00:00

  • Antibacterial Synergy of Silver Nanoparticles with Gentamicin and Chloramphenicol against Enterococcus faecalis.

    abstract:Background:Enterococcus faecalis (Ef) is a multidrug-resistant pathogenic bacteria associated with hospital-acquired infections. Ef is involved in a number of infectious diseases. It generally infects patients with the weekend immune system, i.e. a person mostly acquires Ef infections in the hospital, especially in int...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_120_17

    authors: Katva S,Das S,Moti HS,Jyoti A,Kaushik S

    更新日期:2018-01-01 00:00:00

  • Isolation and Characterisation of a Proanthocyanidin With Antioxidative, Antibacterial and Anti-Cancer Properties from Fern Blechnum orientale.

    abstract:BACKGROUND:Blechnum orientale Linn. (Blechnaceae), a fern, is traditionally used in the treatment of various ailments, such as skin diseases, stomach pain, urinary bladder complaints, and also as a female contraceptive. Previously, we reported a strong radical scavenging activity, antibacterial activity and cytotoxicit...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.197659

    authors: Lai HY,Lim YY,Kim KH

    更新日期:2017-01-01 00:00:00

  • The dose dependent in vitro responses of MCF-7 and MDA-MB-231 cell lines to extracts of Vatica diospyroides symington type SS fruit include effects on mode of cell death.

    abstract:BACKGROUND:Vatica diospyroides type LS is a known source of valuable compounds for cancer treatment, however, in contrast little is known about therapeutic efficacy of type SS. OBJECTIVE:This study focused on in vitro cytotoxicity of these fruit extracts, and the cell death mode they induce in breast cancer cells. MA...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.157718

    authors: Srisawat T,Sukpondma Y,Graidist P,Chimplee S,Kanokwiroon K

    更新日期:2015-05-01 00:00:00

  • Chemical composition and resistance-modifying effect of the essential oil of Lantana camara Linn.

    abstract::In this work, the chemical constituents, antibacterial and modulatory activities of the essential oil of Lantana camara Linn were studied. The essential oil was extracted from the leaves of L. camara by hydrodistillation method using Clevenger's apparatus and its chemical constituents were separated and identified by ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.62890

    authors: Sousa EO,Silva NF,Rodrigues FF,Campos AR,Lima SG,Costa JG

    更新日期:2010-04-01 00:00:00

  • Evaluate the Antigenotoxicity and Anticancer Role of β-Sitosterol by Determining Oxidative DNA Damage and the Expression of Phosphorylated Mitogen-activated Protein Kinases', C-fos, C-jun, and Endothelial Growth Factor Receptor.

    abstract:BACKGROUND:Plant sterols are the major source of micronutrients and have not shown any obvious side effects in human. β-sitosterol is one of the most prevalent phytosterols which have been recorded in ancient medicinal history for its use in the treatment of many chronic diseases, especially cancer. The modulations of ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.197634

    authors: Sharmila R,Sindhu G

    更新日期:2017-01-01 00:00:00

  • Antioxidant activities and fatty acid composition of wild grown myrtle (Myrtus communis L.) fruits.

    abstract::The fruits of eight myrtles, Myrtus communis L. accessions from the Mediterranean region of Turkey were evaluated for their antioxidant activities and fatty acid contents. The antioxidant activities of the fruit extracts were determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and beta-carotene-linoleic acid assa...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.59960

    authors: Serce S,Ercisli S,Sengul M,Gunduz K,Orhan E

    更新日期:2010-01-01 00:00:00

  • A Study on the Chemical Compositions of the Yinqiaosan (Lonicerae and Forsythiae Powder) at Different Time of Later-decoction by Gas Chromatography Mass Spectrometry.

    abstract:BACKGROUND:Yinqiaosan (Lonicerae and Forsythiae Powder), as a famous prescription of Dr. Wu Jutong in Qing dynasty of China, has the effects of diaphoresis cooling, fire-purging, and detoxicaton. It is mainly used in the treatment of influenza, hand-foot-mouth disease, esophagitis, pneumonia, acute tonsillitis, mumps, ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.177911

    authors: Shu Y,Chen Y,Qin K,Liu X,Cai B

    更新日期:2016-04-01 00:00:00

  • Exploring the Cytotoxic Potential of Triterpenoids-enriched Fraction of Bacopa monnieri by Implementing In vitro, In vivo, and In silico Approaches.

    abstract:Background:Bacopa monnieri (BM) is a herbaceous plant traditionally used from time immemorial in Ayurvedic and folklore medicines. We hypothesized that the extract of the whole plant might contain numerous molecules with having antitumor activities that could be very effective in killing of human cancer cells. Objecti...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_397_16

    authors: Mallick MN,Khan W,Parveen R,Ahmad S,Sadaf,Najm MZ,Ahmad I,Husain SA

    更新日期:2017-10-01 00:00:00

  • Quantitative determination of total and individual flavonoids in stems and leaves of Buddleja davidii and Buddleja albiflora.

    abstract:BACKGROUND:Buddleja davidii and B. albiflora are two different original plants of the famous crude medicine "Diaoyangchen". MATERIALS AND METHODS:An ultraviolet-visible spectrophotometric method and a HPLC method were used for the determination of total and individual flavonoids (luteolin and apigenin) contents from t...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.103651

    authors: Ying C,Wan D

    更新日期:2012-10-01 00:00:00

  • Immunomodulatory, Cytotoxicity, and Antioxidant Activities of Roots of Ziziphus mauritiana.

    abstract:AIMS:The study is conducted to evaluate the immunomodulatory, cytotoxicity, and antioxidant potential of Ziziphus mauritiana (Rhamnaceae). Phytochemical analysis of Z. mauritiana revealed the presence of alkaloids, anthraquinone glycoside, cardiac glycoside, saponin, tannin, and flavonoids. METHODOLOGY:The cytotoxicit...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_398_16

    authors: Afzal S,Batool M,Ch BA,Ahmad A,Uzair M,Afzal K

    更新日期:2017-07-01 00:00:00

  • Secondary Metabolites from Leaves of Manilkara subsericea (Mart.) Dubard.

    abstract:BACKGROUND:Manilkara subsericea (Sapotaceae) is a species widely spread in the sandbanks of Restinga de Jurubatiba National Park (Rio de Janeiro, Brazil). It is commonly known as "maçaranduba", "maçarandubinha" and "guracica", being used in this locality as food, and timber. However, M. subsericea remains almost unexpl...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.172957

    authors: de Almeida FB,Fernandes CP,Romao W,Vanini G,Costa HB,França HS,Santos MG,Carvalho JC,Falcão DQ,Rocha L

    更新日期:2015-10-01 00:00:00

  • In vitro α-amylase and α-glucosidase inhibitory effects and cytotoxic activity of Albizia antunesiana extracts.

    abstract:CONTEXT:Diabetes mellitus is a chronic disease, and its incidence is tremendously increasing globally. Decreasing postprandial hyperglycemia by retarding glucose absorption through inhibiting carbohydrates digesting enzymes (α-amylase and α-glucosidase) is one of many approaches used for the management of this disease....

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.166018

    authors: Chipiti T,Ibrahim MA,Singh M,Islam MS

    更新日期:2015-10-01 00:00:00

  • Optimization of Polysaccharide Ultrasonic Extraction Conditions Using Purple Sweet Potato Tubers Based on Free Radical Scavenging and Glycosylation Inhibitory Bioactivities.

    abstract:BACKGROUND:The purple sweet potato, Ipomoea batatas, belongs to the family Convolvulaceae. It is one of the most widely consumed tubers in Asia and is found in many dishes. Many people with diabetes eat purple sweet potato tubers to help reduce blood glucose in China. OBJECTIVE:To predict the ultrasonic conditions for...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.211044

    authors: Guo H,Kong F,Yan C

    更新日期:2017-07-01 00:00:00

  • Neuroprotective activity of the methanolic extract of Lonicera japonica in glutamate-injured primary rat cortical cells.

    abstract:BACKGROUND:We previously reported that the extracts of several Korean medicinal plants showed neuroprotective activity in glutamate-injured primary culutres of rat cortical cells. OBJECTIVE:Among them, the effect of the methanolic extract of Lonicera japonica flower on the glutamate-induced neuronal cell death and its...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.90404

    authors: Weon JB,Yang HJ,Lee B,Yun BR,Ahn JH,Lee HY,Ma CJ

    更新日期:2011-10-01 00:00:00

  • New free radical scavenging neolignans from fruits of Piper attenuatum.

    abstract:OBJECTIVE:The aim was to study and identify free radicals scavenging and antihyperglycemic principles in fruit of Piper attenuatum. MATERIALS AND METHODS:Bioassay guided identification of extracts possessing potent free radical scavenging activity, and isolation of compounds was done. Chloroform extract of P. attenuat...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/0973-1296.153063

    authors: Reddy SD,Siva B,Poornima B,Kumar DA,Tiwari AK,Ramesh U,Babu KS

    更新日期:2015-04-01 00:00:00

  • Methanolic Extract of Costus pictus D. DON Induces Cytotoxicity in Liver Hepatocellular Carcinoma Cells Mediated by Histone Deacetylase Inhibition.

    abstract:Background:Leaves of Costus pictus D. Don, (insulin plant) are used as dietary supplement for the treatment of diabetes. Objective:The antidiabetic activity of this plant is well documented, but its activity on different cell types and mechanism remains unknown. Thus, the present study evaluates the cytotoxicity of C....

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_524_16

    authors: Neethu PV,Suthindhiran K,Jayasri MA

    更新日期:2017-10-01 00:00:00

  • Topical Delivery of Withania somnifera Crude Extracts in Niosomes and Solid Lipid Nanoparticles.

    abstract:Background:Withania somnifera is a medicinal plant native to India and is known to have anticancer properties. It has been investigated for its anti-melanoma properties, and since melanoma presents on the skin, it is prudent to probe the use of W. somnifera in topical formulations. To enhance topical drug delivery and ...

    journal_title:Pharmacognosy magazine

    pub_type: 杂志文章

    doi:10.4103/pm.pm_489_16

    authors: Chinembiri TN,Gerber M,du Plessis LH,du Preez JL,Hamman JH,du Plessis J

    更新日期:2017-10-01 00:00:00