当前位置： SCI文献检索 > MOLECULAR AND CELLULAR ENDOCRINOLOGY期刊下所有文献 > Vasopressin, angiotensin and adrenergic receptors of rat liver Golgi fractions--molecular weight of the angiotensin-receptor irreversible complex after in vitro and in vivo labelling.

Vasopressin, angiotensin and adrenergic receptors of rat liver Golgi fractions--molecular weight of the angiotensin-receptor irreversible complex after in vitro and in vivo labelling.

Abstract：

:The binding of vasopressin, angiotensin II and prazosin (alpha 1-adrenergic antagonist) to purified heavy (GH) and (intermediate + light) (GI + L) rat liver Golgi fractions was studied. The three types of ligands showed a saturable and specific binding in Golgi fractions; the maximal specific binding of [3H]vasopressin, [3H]prazosin and [125I]Sar-N3-Phe-angiotensin II was respectively 5-10%, 20-30% and 30-40% of that detected in purified plasma membranes. The apparent binding affinities of the three ligands were the same whether determined in Golgi fractions or plasma membranes. The presence of vasopressin, alpha 1-adrenergic and angiotensin receptors in very different proportions, as compared to the amount of receptor detected in plasma membranes, in GH and GI + L Golgi fractions was not compatible with the idea that a plasma membrane impurity accounted for the detection of receptor in the purified intracellular particulate fractions. In vivo injection of [125I]Sar-N3-Phe-angiotensin II resulted in a receptor-mediated endocytosis of the iodo-angiotensin analog into the GH and GI + L Golgi fractions. The apparent molecular weight of the irreversible complex, [125I]angiotensin-receptor, was estimated in subcellular fractions using SDS-PAGE electrophoresis. This value was identical after either in vivo or in vitro labelling (MW = 63,000) and was indistinguishable from the molecular weight of the irreversible hormone receptor complex present in the plasma membranes.

journal_name

Mol Cell Endocrinol

journal_title

Molecular and cellular endocrinology

authors

Cantau B,Guillemette G,Chicot D,Devilliers G

doi

10.1016/0303-7207(87)90119-5

subject

Has Abstract

pub_date

1987-05-01 00:00:00

pages

59-69

issue

1-2

eissn

0303-7207

issn

1872-8057

journal_volume

51

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR ENDOCRINOLOGY文献大全
  • New methods for investigating experimental human adrenal tumorigenesis.

    abstract：:Adenomas and nodules of the human adrenal cortex are common, whereas adrenocortical carcinomas are rare. Genes such as IGF2 have been suggested to be important in human adrenocortical tumorigenesis but their role has not been directly investigated. We describe here elements of a system in which hypotheses concerning t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2008.10.046

    authors： Cardoso CC,Bornstein SR,Hornsby PJ

    更新日期：2009-03-05 00:00:00

  • Impact of endocrine-disrupting compounds (EDCs) on female reproductive health.

    abstract：:Evidence is accumulating that environmental chemicals (ECs) including endocrine-disrupting compounds (EDCs) can alter female reproductive development, fertility and onset of menopause. While not as clearly defined as in the male, this set of abnormalities may constitute an Ovarian Dysgenesis Syndrome with at least som...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2011.10.021

    authors： Fowler PA,Bellingham M,Sinclair KD,Evans NP,Pocar P,Fischer B,Schaedlich K,Schmidt JS,Amezaga MR,Bhattacharya S,Rhind SM,O'Shaughnessy PJ

    更新日期：2012-05-22 00:00:00

  • Intratesticular regulation of testosterone secretion: comparison of the effects and interactions of hCG, an LHRH agonist and testicular interstitial fluid on Leydig cell testosterone secretion in vitro.

    abstract：:The stimulatory effects on Leydig cell testosterone secretion of a polypeptide(s) factor present in testicular interstitial fluid (IF) were compared with those of hCG and an LHRH agonist (LHRH-A). The actions of IF and LHRH-A were similar in showing (1) a delayed onset of action, (2) enhancement of testosterone produc...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(85)90029-2

    authors： Sharpe RM

    更新日期：1985-07-01 00:00:00

  • Melanocortin receptors and their accessory proteins.

    abstract：:The melanocortin receptor family consists of 5 members which belong to the GPCR superfamily. Their specific ligands, the melanocortins are peptide hormones which are formed by the proteolytic cleavage of the proopiomelanocortin (POMC) protein. It is now recognised that certain GPCRs require accessory proteins for thei...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2010.07.015

    authors： Cooray SN,Clark AJ

    更新日期：2011-01-15 00:00:00

  • Genetic modifications of mouse proopiomelanocortin peptide processing.

    abstract：:Pro-opiomelanocortin (POMC) is a prohormone which undergoes extensive tissue and cell specific post-translational processing producing a number of active peptides with diverse biological roles ranging from control of adrenal function to pigmentation to the regulation of feeding. One approach to unraveling the complexi...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2010.12.032

    authors： Costa JL,Forbes S,Brennan MB,Hochgeschwender U

    更新日期：2011-04-10 00:00:00

  • Parathyroid receptor gene expression by epiphyseal growth plates in rickets and tibial dyschondroplasia.

    abstract：:PTH/PTHrP receptor gene expression was evaluated in situ in avian epiphyseal growth plates taken from normal, rachitic and tibial dyschondroplasia (TD) afflicted chicks induced by thiram or by genetic selection. In the normal growth plates, PTH/PTHrP receptor gene expression was localized to the maturation zone as dem...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(98)00231-7

    authors： Ben-Bassat S,Genina O,Lavelin I,Leach RM,Pines M

    更新日期：1999-03-25 00:00:00

  • A monoclonal antibody which inhibits the biological activity of rat prolactin, but not prolactin from other species.

    abstract：:Monoclonal antibodies generated to ovine prolactin were screened for their ability to neutralize the biological activity of prolactin from several species. By Western blot analysis, antibody 6F11 cross-reacted strongly with prolactin in homogenates of anterior pituitary glands from squirrel monkey, sheep and rat. In a...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(90)90249-8

    authors： Scammell JG,Wear LB,Von Haven R

    更新日期：1990-06-18 00:00:00

  • Identification of growth hormone binding protein in rat serum.

    abstract：:The present report describes the initial characterization of a specific, high-affinity growth hormone binding protein (GH-BP) in adult male rat serum. GH-BP activity was measured by incubation of rat serum with [125I]hGH and [125I]rGH and separation of bound from free GH by dextran-coated charcoal. [125I]hGH binding t...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(90)90159-6

    authors： Amit T,Barkey RJ,Bick T,Hertz P,Youdim MB,Hockberg Z

    更新日期：1990-04-17 00:00:00

  • Anti-Müllerian hormone and its role in ovarian function.

    abstract：:Anti-Müllerian hormone (AMH) is expressed after birth in the ovary in the granulosa cells of healthy, small growing follicles. We have shown that AMH affects two important regulatory steps during folliculogenesis. At initial recruitment, AMH inhibits recruitment of primordial follicles into the growing pool, while at ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2003.09.024

    authors： Gruijters MJ,Visser JA,Durlinger AL,Themmen AP

    更新日期：2003-12-15 00:00:00

  • Regulated expression of pancreatic renin-angiotensin system in experimental pancreatitis.

    abstract：:Our previous studies have provided evidence for the existence of an intrinsic renin-angiotensin system (RAS) in the rat pancreas, which may play a role in the regulation of pancreatic microcirculation and ductal secretion. Such a pancreatic RAS has recently shown to be activated by chronic hypoxia. The activation of a...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(00)00275-6

    authors： Leung PS,Chan WP,Nobiling R

    更新日期：2000-08-30 00:00:00

  • Further characterization of the androgen receptor in rat testis.

    abstract：:Immature rat testes contain a specific binding protein for testosterone (T) and 5alpha-dihyrotestosterone (DHT) with physico-chemical properties similar to the cytoplasmic androgen receptors in the epididymis and ventral prostate but different from the testicular androgen-binding protein (ABP). Like the androgen recep...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(76)90058-7

    authors： McLean WS,Smith AA,Hansson V,Naess O,Nayfeh SN,French FS

    更新日期：1976-03-01 00:00:00

  • Synthesis and secretion of human chorionic gonadotropin and its subunits in choriocarcinoma cells: a comparative study with normal placental cells.

    abstract：:The human choriocarcinoma cell line, BeWo, synthesizes the glycoprotein hormone, human chorionic gonadotropin (hCG). We have undertaken this study to compare the synthesized and secreted forms of hCG and their alpha- and beta-subunits in cell cultures of BeWo cells to those forms of normal placental cells by immunobin...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(90)90008-v

    authors： Takeuchi Y,Sakakibara R,Ishiguro M

    更新日期：1990-03-05 00:00:00

  • Endocytosis of the lutropin receptor is mediated by a low affinity binding site.

    abstract：:Porcine Leydig cells in primary culture were incubated to equilibrium with increasing doses of either porcine luteinizing hormone (pLH) or human chorionic gonadotropin (hCG). Then, free and total high affinity gonadotropin receptors on the cell surface were dosed with [125I]hCG as tracer. In isotonic high salt medium,...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(91)90263-r

    authors： Genty N,Salesse R,Jallal B,Garnier J

    更新日期：1991-04-01 00:00:00

  • Angiotensin II-stimulated changes in calcium metabolism in cultured glomerulosa cells.

    abstract：:Studies were performed to evaluate the relationships between the effects of angiotensin II on calcium metabolism and cytosolic free calcium concentration in primary monolayer cultures of bovine adrenal glomerulosa cells. As noted previously (Kramer (1988) Am. J. Physiol. (in press], angiotensin II produced rapid dose-...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(88)90179-7

    authors： Kramer RE

    更新日期：1988-12-01 00:00:00

  • Target of cockroach allatostatin in the pathway of juvenile hormone biosynthesis.

    abstract：:Allatostatins, peptides with an YXFGL-amide C-terminus, cause a rapid and reversible inhibition of the sesquiterpenoid juvenile hormone III biosynthesis by cockroach corpora allata in vitro. Inhibition is relieved by farnesol and by mevalonate, and neither HMG-CoA reductase nor HMG-CoA synthesis (HMG-CoA synthase and ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(96)03825-7

    authors： Sutherland TD,Feyereisen R

    更新日期：1996-07-01 00:00:00

  • Directional secretion and transport of leptin and expression of leptin receptor isoforms in human placental BeWo cells.

    abstract：:Placental leptin secretion has important implications for maternal adaptation to pregnancy, fetal growth and development, and local autocrine/paracrine actions within trophoblast. In this study we used a cell culture insert model to examine directional secretion of leptin from the basal and apical surfaces of human ch...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2005.05.003

    authors： Wyrwoll CS,Mark PJ,Waddell BJ

    更新日期：2005-09-28 00:00:00

  • Function and expression of somatostatin receptors of the endocrine pancreas.

    abstract：:Somatostatin (SST) regulates multiple biological processes via five genetically distinct, G-protein coupled receptors. Clinical interest in therapy for neuroendocrine and metabolic disorders has resulted in the development of new tools for exploring the function of somatostatin receptors (SSTRs). The development of hi...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.mce.2008.02.007

    authors： Strowski MZ,Blake AD

    更新日期：2008-05-14 00:00:00

  • The cell surface progesterone receptor which stimulates calcium influx in human sperm is unlike the A ring reduced steroid site on the GABAA receptor/chloride channel.

    abstract：:Progesterone elicits a rapid, transient calcium influx in sperm that is a prerequisite for the progesterone-induced acrosome reaction. The possibility that the GABAA receptor/chloride channel was the receptor that mediated the progesterone-induced calcium influx in human sperm was examined. A-ring reduced 3 alpha-hydr...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(94)90126-0

    authors： Blackmore PF,Im WB,Bleasdale JE

    更新日期：1994-09-01 00:00:00

  • Molecular and functional studies of 4 candidate loci in Pendred syndrome and nonsyndromic hearing loss.

    abstract：:Patients with PS or non-syndromic deafness were submitted to genetic/functional analyzes of SLC26A4, of its binding domain for FOXI1 (FOXI1-DBD), of the transcription activator FOXI1, and of the potassium channel KCNJ10. SLC26A4 was the most frequently mutated gene. An altered intracellular localization with immunocyt...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2012.01.013

    authors： Cirello V,Bazzini C,Vezzoli V,Muzza M,Rodighiero S,Castorina P,Maffini A,Bottà G,Persani L,Beck-Peccoz P,Meyer G,Fugazzola L

    更新日期：2012-04-04 00:00:00

  • Characterization of the activation function-2 domain of the human 1,25-dihydroxyvitamin D3 receptor.

    abstract：:In this study, we determined the ligand-dependent activation function domain 2 (AF-2) of the human vitamin D receptor (hVDR) and characterized it using site-directed mutagenesis. A single mutation at glutamic acid-420 (E420Q) and an additional mutation at leucine-417 (L417A-E420Q) eliminated ligand-dependent transcrip...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0303-7207(98)00077-x

    authors： Nakajima S,Yamagata M,Sakai N,Ozono K

    更新日期：1998-04-30 00:00:00

  • Thyroid hormone stimulates the production of insulin-like growth factor I (IGF-I) by immature rat Sertoli cells.

    abstract：:The effects of thyroid hormone on insulin-like growth factor I (IGF-I) production by Sertoli cells isolated from immature rats have been investigated. In Sertoli cells from hypothyroid rats the production of IGF-I was significantly lower than in controls and was greatly stimulated by the administration of triiodothyro...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(90)90170-d

    authors： Palmero S,Prati M,Barreca A,Minuto F,Giordano G,Fugassa E

    更新日期：1990-01-02 00:00:00

  • Regulation of monocyte chemotactic protein-1 gene expression in human endometrial cells in cultures.

    abstract：:Bone marrow-derived leukocytes are present in human endometrium/decidua and are believed to serve a variety of functions in this tissue. The number and type of leukocytes in endometrium/decidua vary with the hormonal milieu of the ovarian cycle, with blastocyst implantation, and during pregnancy. The factors that regu...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(94)03442-v

    authors： Arici A,MacDonald PC,Casey ML

    更新日期：1995-02-01 00:00:00

  • Hypoxia regulation of expression and angiogenic effects of vasoactive intestinal peptide (VIP) and VIP receptors in LNCaP prostate cancer cells.

    abstract：:Vascular endothelial growth factor (VEGF) is a main factor promoting neovascularization (angiogenesis) of solid tumours as prostate carcinoma. Hypoxia stimulates VEGF gene expression by activating the hypoxia-inducible factor-1 (HIF-1alpha). In the present study, the hypoxia-mimicking agent Ni(2+) induced vasoactive i...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2006.02.004

    authors： Collado B,Sánchez-Chapado M,Prieto JC,Carmena MJ

    更新日期：2006-04-25 00:00:00

  • Distribution and molecular forms of immunoreactive bombesin in the ovine median eminence.

    abstract：:The distribution and molecular forms of bombesin-like immunoreactivity (BLI) were determined in the ovine median eminence using a new, C-terminally directed antiserum. BLI was confined to neurons of the external zone of the median eminence, near small blood vessels, many of which also were immunoreactive for CRF. Each...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(87)90180-8

    authors： Giraud AS,Smith AI,Rundle SE

    更新日期：1987-10-01 00:00:00

  • Ovine 11 beta-hydroxysteroid dehydrogenase type 2 gene predicts a protein distinct from that deduced by the cloned kidney cDNA at the C-terminus.

    abstract：:The gene encoding ovine 11 beta-hydroxysteroid dehydrogenase type 2 (11 beta-HSD2) was cloned and characterized. This gene consists of five exons and is greater than 4 kb in length. It contains an open reading frame of 1215 bp, which encodes a protein of 404 amino acids with a predicted MW of 44 kDa. The deduced ovine...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(96)03775-6

    authors： Campbell LE,Yu M,Yang K

    更新日期：1996-05-17 00:00:00

  • Characterization of macaque 3 beta-hydroxy-5-ene steroid dehydrogenase/delta 5-delta 4 isomerase: structure and expression in steroidogenic and peripheral tissues in primate.

    abstract：:The conversion of 3 beta-hydroxy-5-ene steroids by the enzyme complex 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD) is an obligatory step in the biosynthesis of all classes of hormonal steroids in classical steroidogenic as well as in peripheral tissues. To develop a model more closely rel...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(91)90224-g

    authors： Simard J,Melner MH,Breton N,Low KG,Zhao HF,Periman LM,Labrie F

    更新日期：1991-02-01 00:00:00

  • Calcitonin increases transcription of parathyroid hormone-related protein via cAMP.

    abstract：:Transcriptional regulation of the human parathyroid hormone-related protein (PTHrP) gene by calcitonin was examined in a lung cancer line (BEN cells). Northern analysis demonstrated that calcitonin caused a rapid 4.5-fold elevation in PTHrP mRNA. Transient transfection of a construct containing 1119 base pairs of the ...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(93)90045-l

    authors： Chilco PJ,Gerardi JM,Kaczmarczyk SJ,Chu S,Leopold V,Zajac JD

    更新日期：1993-07-01 00:00:00

  • Androgens differentially regulate the expression of NEDD4L transcripts in LNCaP human prostate cancer cells.

    abstract：:The NEDD4L gene encodes a ubiquitin ligase that targets the epithelial sodium channel for degradation. A search for transcripts whose levels increase following androgen treatment of LNCaP human prostate cancer cells led to the isolation of three new NEDD4L transcripts designated NEDD4Lf, NEDD4Lg and NEDD4Lh. The three...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.mce.2003.08.009

    authors： Qi H,Grenier J,Fournier A,Labrie C

    更新日期：2003-11-28 00:00:00

  • Identification of a minor Tg mRNA transcript in RNA from normal and goitrous thyroids.

    abstract：:Polymerase chain reaction (PCR) amplification of nt 4502 to nt 5184 of the thyroglobulin (Tg) mRNA from several patients, with or without elevated serum thyrotropin (TSH), showed a predominant fragment of the expected size (683 bp) and a minor fragment of 512 bp. The sequence of this minor fragment revealed that 171 b...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(92)90087-m

    authors： Targovnik HM,Cochaux P,Corach D,Vassart G

    更新日期：1992-03-01 00:00:00

  • Cytochrome P-450 and the aromatization of 16alpha-hydroxytestosterone and androstenedione by human placental microsomes.

    abstract：:When measured in vitro using human placental microsomal preparations, the aromatization of 16alpha-hydroxytestosterone to estriol and androstenedione to estrone and estradiol proceeds at almost identical initial rates. Important differences between 16alpha-hydroxytestosterone and adrostenedione aromatization are evide...

    journal_title：Molecular and cellular endocrinology

    pub_type： 杂志文章

    doi：10.1016/0303-7207(76)90010-1

    authors： Canick JA,Ryan KJ

    更新日期：1976-12-01 00:00:00