Abstract：
:Pro-opiomelanocortin (POMC) is a prohormone which undergoes extensive tissue and cell specific post-translational processing producing a number of active peptides with diverse biological roles ranging from control of adrenal function to pigmentation to the regulation of feeding. One approach to unraveling the complexities of the POMC system is to engineer mouse mutants which lack specific POMC peptides. We describe here the design, generation, validation, and preliminary analysis of one such partial POMC mutant specifically lacking α-MSH. In contrast to POMC null mutant mice, mice lacking α-MSH in the presence of all other POMC peptides maintain adrenal structures and produce corticosterone comparable to wildtype littermates; however, they still have decreased levels of aldosterone, as found in POMC null mutant mice. Our findings demonstrate that α-MSH is not needed for maintenance of adrenal structure or for corticosterone production, but is needed for aldosterone production. These data demonstrate that mouse strains generated with precise genetic modifications of POMC peptide processing can answer questions about POMC peptide function. Further analysis of this and additional strains of mice with modified POMC peptide processing patterns will open up a novel avenue for studying the roles of individual POMC peptides.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Costa JL,Forbes S,Brennan MB,Hochgeschwender Udoi
10.1016/j.mce.2010.12.032subject
Has Abstractpub_date
2011-04-10 00:00:00pages
14-22issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(10)00625-8journal_volume
336pub_type
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