Abstract:
:NaK and other non-selective channels are able to conduct both sodium (Na+) and potassium (K+) with equally high efficiency. In contrast to previous crystallographic results, we show that the selectivity filter (SF) of NaK in native-like lipid membranes adopts two distinct conformations that are stabilized by either Na+ or K+ ions. The atomic differences of these conformations are resolved by solid-state NMR (ssNMR) spectroscopy and molecular dynamics (MD) simulations. Besides the canonical K+ permeation pathway, we identify a side entry ion-conduction pathway for Na+ permeation unique to NaK. Moreover, under otherwise identical conditions ssNMR spectra of the K+ selective NaK mutant (NaK2K) reveal only a single conformational state. Therefore, we propose that structural plasticity within the SF and the selection of these conformations by different ions are key molecular determinants for highly efficient conduction of different ions in non-selective cation channels.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Shi C,He Y,Hendriks K,de Groot BL,Cai X,Tian C,Lange A,Sun Hdoi
10.1038/s41467-018-03179-ysubject
Has Abstractpub_date
2018-02-19 00:00:00pages
717issue
1issn
2041-1723pii
10.1038/s41467-018-03179-yjournal_volume
9pub_type
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