Quantitative volumetric Raman imaging of three dimensional cell cultures.

abstract：:Caffeine is a major component of xanthine alkaloids and commonly consumed in many popular beverages. Due to its occasional side effects, reduction of caffeine in a natural way is of great importance and economic significance. Recent studies reveal that caffeine can be converted into non-stimulatory theacrine in the ra...

journal_title：Nature communications

authors： Zhang YH,Li YF,Wang Y,Tan L,Cao ZQ,Xie C,Xie G,Gong HB,Sun WY,Ouyang SH,Duan WJ,Lu X,Ding K,Kurihara H,Hu D,Zhang ZM,Abe I,He RR

更新日期：2020-03-19 00:00:00

abstract：:Homologous recombination-mediated gene targeting has greatly contributed to genetic analysis in a wide range of species, but is highly inefficient in human cells because of overwhelmingly frequent random integration events, whose molecular mechanism remains elusive. Here we show that DNA polymerase θ, despite its mino...

journal_title：Nature communications

authors： Saito S,Maeda R,Adachi N

更新日期：2017-07-11 00:00:00

abstract：:UV vision is prevalent, but we know little about its utility in common general tasks, as in resolving habitat structure. Here we visualize vegetated habitats using a multispectral camera with channels mimicking bird photoreceptor sensitivities across the UV-visible spectrum. We find that the contrast between upper and...

journal_title：Nature communications

authors： Tedore C,Nilsson DE

更新日期：2019-01-22 00:00:00

abstract：:Owing to their pathogenical role and unique ability to exist both as soluble proteins and transmembrane complexes, pore-forming toxins (PFTs) have been a focus of microbiologists and structural biologists for decades. PFTs are generally secreted as water-soluble monomers and subsequently bind the membrane of target ce...

journal_title：Nature communications

authors： Iacovache I,De Carlo S,Cirauqui N,Dal Peraro M,van der Goot FG,Zuber B

更新日期：2016-07-13 00:00:00

abstract：:DNA-based nanopores are synthetic biomolecular membrane pores, whose geometry and chemical functionality can be tuned using the tools of DNA nanotechnology, making them promising molecular devices for applications in single-molecule biosensing and synthetic biology. Here we introduce a large DNA membrane channel with ...

journal_title：Nature communications

authors： Krishnan S,Ziegler D,Arnaut V,Martin TG,Kapsner K,Henneberg K,Bausch AR,Dietz H,Simmel FC

更新日期：2016-09-23 00:00:00

abstract：:Alteration of the gut microbiota has been associated with different gastrointestinal disorders. Normobiosis restoration by faecal microbiota transplantation (FMT) is considered a promising therapeutic approach, even if the mechanisms underlying its efficacy are at present largely unknown. Here we sought to elucidate t...

journal_title：Nature communications

authors： Burrello C,Garavaglia F,Cribiù FM,Ercoli G,Lopez G,Troisi J,Colucci A,Guglietta S,Carloni S,Guglielmetti S,Taverniti V,Nizzoli G,Bosari S,Caprioli F,Rescigno M,Facciotti F

更新日期：2018-12-05 00:00:00

abstract：:Acquisition of pluripotency by somatic cells is a striking process that enables multicellular organisms to regenerate organs. This process includes silencing of genes to erase original tissue memory and priming of additional cell type specification genes, which are then poised for activation by external signal inputs....

journal_title：Nature communications

authors： Ishihara H,Sugimoto K,Tarr PT,Temman H,Kadokura S,Inui Y,Sakamoto T,Sasaki T,Aida M,Suzuki T,Inagaki S,Morohashi K,Seki M,Kakutani T,Meyerowitz EM,Matsunaga S

更新日期：2019-04-16 00:00:00

abstract：:Processive chitinase is a linear molecular motor which moves on the surface of crystalline chitin driven by processive hydrolysis of single chitin chain. Here, we analyse the mechanism underlying unidirectional movement of Serratia marcescens chitinase A (SmChiA) using high-precision single-molecule imaging, X-ray cry...

journal_title：Nature communications

authors： Nakamura A,Okazaki KI,Furuta T,Sakurai M,Iino R

更新日期：2018-09-19 00:00:00

abstract：:To avoid dangerous climate change, new technologies must remove billions of tonnes of CO2 from the atmosphere every year by mid-century. Here we detail a land-based enhanced weathering cycle utilizing magnesite (MgCO3) feedstock to repeatedly capture CO2 from the atmosphere. In this process, MgCO3 is calcined, produci...

journal_title：Nature communications

authors： McQueen N,Kelemen P,Dipple G,Renforth P,Wilcox J

更新日期：2020-07-03 00:00:00

abstract：:During animal cell division, the central spindle, an anti-parallel microtubule bundle structure formed between segregating chromosomes during anaphase, cooperates with astral microtubules to position the cleavage furrow. Because the central spindle is the only structure linking the two halves of the mitotic spindle, i...

journal_title：Nature communications

authors： Lee KY,Esmaeili B,Zealley B,Mishima M

更新日期：2015-06-19 00:00:00

abstract：:The diploid nature of the human genome is neglected in many analyses done today, where a genome is perceived as a set of unphased variants with respect to a reference genome. This lack of haplotype-level analyses can be explained by a lack of methods that can produce dense and accurate chromosome-length haplotypes at ...

journal_title：Nature communications

authors： Porubsky D,Garg S,Sanders AD,Korbel JO,Guryev V,Lansdorp PM,Marschall T

更新日期：2017-11-03 00:00:00

abstract：:In vitro fabrication of functional vascularized three-dimensional tissues has been a long-standing objective in the field of tissue engineering. Here we report a technique to engineer cardiac tissues with perfusable blood vessels in vitro. Using resected tissue with a connectable artery and vein as a vascular bed, we ...

journal_title：Nature communications

authors： Sekine H,Shimizu T,Sakaguchi K,Dobashi I,Wada M,Yamato M,Kobayashi E,Umezu M,Okano T

更新日期：2013-01-01 00:00:00

abstract：:Use of aromatase inhibitors (AIs), exemestane, letrozole and anastrozole, for breast cancer therapy is associated with severe pain symptoms, the underlying mechanism of which is unknown. The electrophilic nature of AIs suggests that they may target the transient receptor potential ankyrin 1 (TRPA1) channel, a major pa...

journal_title：Nature communications

authors： Fusi C,Materazzi S,Benemei S,Coppi E,Trevisan G,Marone IM,Minocci D,De Logu F,Tuccinardi T,Di Tommaso MR,Susini T,Moneti G,Pieraccini G,Geppetti P,Nassini R

更新日期：2014-12-08 00:00:00

abstract：:It is well known that high-risk human papilloma virus (HR-HPV) infection is strongly associated with cervical cancer and E7 was identified as one of the key initiators in HPV-mediated carcinogenesis. Here we show that lactate dehydrogenase A (LDHA) preferably locates in the nucleus in HPV16-positive cervical tumors du...

journal_title：Nature communications

authors： Liu Y,Guo JZ,Liu Y,Wang K,Ding W,Wang H,Liu X,Zhou S,Lu XC,Yang HB,Xu C,Gao W,Zhou L,Wang YP,Hu W,Wei Y,Huang C,Lei QY

更新日期：2018-10-24 00:00:00

abstract：:It is well known that a topologically protected gapless state appears at an interface between a topological insulator and an ordinary insulator; however, the physics of the interface between a topological insulator and a metal has largely been left unexplored. Here we report a novel phenomenon termed topological proxi...

journal_title：Nature communications

authors： Shoman T,Takayama A,Sato T,Souma S,Takahashi T,Oguchi T,Segawa K,Ando Y

更新日期：2015-03-12 00:00:00

abstract：:Over the recent decades there has been tremendous progress in understanding and controlling friction between surfaces in relative motion. However the complex nature of the involved processes has forced most of this work to be of rather empirical nature. Two very distinctive physical systems, hard two-dimensional layer...

journal_title：Nature communications

authors： Vanossi A,Bechinger C,Urbakh M

更新日期：2020-09-16 00:00:00

abstract：:Pseudomonas aeruginosa, an opportunistic pathogen of humans, uses quorum sensing (QS) to regulate the production of extracellular products that can benefit all members of the population. P. aeruginosa can police QS-deficient cheaters by producing hydrogen cyanide, which is also QS regulated; however, the mechanism by ...

journal_title：Nature communications

authors： Yan H,Asfahl KL,Li N,Sun F,Xiao J,Shen D,Dandekar AA,Wang M

更新日期：2019-11-01 00:00:00

abstract：:Photoelectrochemical (PEC) water splitting promises a solution to the problem of large-scale solar energy storage. However, its development has been impeded by the poor performance of photoanodes, particularly in their capability for photovoltage generation. Many examples employing photovoltaic modules to correct the ...

journal_title：Nature communications

authors： Jang JW,Du C,Ye Y,Lin Y,Yao X,Thorne J,Liu E,McMahon G,Zhu J,Javey A,Guo J,Wang D

更新日期：2015-06-16 00:00:00

abstract：:Tumour cells secrete exosomes that are involved in the remodelling of the tumour-stromal environment and promoting malignancy. The mechanisms governing tumour exosome release, however, remain incompletely understood. Here we show that tumour cell exosomes secretion is controlled by pyruvate kinase type M2 (PKM2), whic...

journal_title：Nature communications

authors： Wei Y,Wang D,Jin F,Bian Z,Li L,Liang H,Li M,Shi L,Pan C,Zhu D,Chen X,Hu G,Liu Y,Zhang CY,Zen K

更新日期：2017-01-09 00:00:00

abstract：:Endolysosomal organelles play a key role in trafficking, breakdown and receptor-mediated recycling of different macromolecules such as low-density lipoprotein (LDL)-cholesterol, epithelial growth factor (EGF) or transferrin. Here we examine the role of two-pore channel (TPC) 2, an endolysosomal cation channel, in thes...

journal_title：Nature communications

authors： Grimm C,Holdt LM,Chen CC,Hassan S,Müller C,Jörs S,Cuny H,Kissing S,Schröder B,Butz E,Northoff B,Castonguay J,Luber CA,Moser M,Spahn S,Lüllmann-Rauch R,Fendel C,Klugbauer N,Griesbeck O,Haas A,Mann M,Bracher F,T

更新日期：2014-08-21 00:00:00

abstract：:The NASA MErcury Surface, Space ENvironment, GEochemistry and Ranging (MESSENGER) spacecraft entered orbital phase around Mercury on 18 March 2011. A surprising consistent feature in the data returned is large-scale vortices that form exclusively on the dusk side of the magnetosphere. Here we present global kinetic hy...

journal_title：Nature communications

authors： Paral J,Rankin R

更新日期：2013-01-01 00:00:00

abstract：:Forests are of major importance to human society, contributing several crucial ecosystem services. Biodiversity is suggested to positively influence multiple services but evidence from natural systems at scales relevant to management is scarce. Here, across a scale of 400,000 km(2), we report that tree species richnes...

journal_title：Nature communications

authors： Gamfeldt L,Snäll T,Bagchi R,Jonsson M,Gustafsson L,Kjellander P,Ruiz-Jaen MC,Fröberg M,Stendahl J,Philipson CD,Mikusiński G,Andersson E,Westerlund B,Andrén H,Moberg F,Moen J,Bengtsson J

更新日期：2013-01-01 00:00:00

abstract：:Immunotherapy is emerging as a new pillar of cancer treatment with potential to cure. However, many patients still fail to respond to these therapies. Among the underlying factors, an immunosuppressive tumor microenvironment (TME) plays a major role. Here we show that monocyte-mediated gene delivery of IFNα inhibits l...

journal_title：Nature communications

authors： Escobar G,Barbarossa L,Barbiera G,Norelli M,Genua M,Ranghetti A,Plati T,Camisa B,Brombin C,Cittaro D,Annoni A,Bondanza A,Ostuni R,Gentner B,Naldini L

更新日期：2018-07-24 00:00:00

abstract：:Androgen-ablation therapies, which are the standard treatment for metastatic prostate cancer, invariably lead to acquired resistance. Hence, a systematic identification of additional drivers may provide useful insights into the development of effective therapies. Numerous microRNAs that are critical for metastasis are...

journal_title：Nature communications

authors： Xue M,Liu H,Zhang L,Chang H,Liu Y,Du S,Yang Y,Wang P

更新日期：2017-04-11 00:00:00

abstract：:Intercellular signaling is indispensable for single cells to form complex biological structures, such as biofilms, tissues and organs. The genetic tools available for engineering intercellular signaling, however, are quite limited. Here we exploit the chemical diversity of biological small molecules to de novo design ...

journal_title：Nature communications

authors： Du P,Zhao H,Zhang H,Wang R,Huang J,Tian Y,Luo X,Luo X,Wang M,Xiang Y,Qian L,Chen Y,Tao Y,Lou C

更新日期：2020-08-24 00:00:00

abstract：:Fibrosis due to extracellular matrix (ECM) secretion from myofibroblasts complicates many chronic liver diseases causing scarring and organ failure. Integrin-dependent interaction with scar ECM promotes pro-fibrotic features. However, the pathological intracellular mechanism in liver myofibroblasts is not completely u...

journal_title：Nature communications

authors： Martin K,Pritchett J,Llewellyn J,Mullan AF,Athwal VS,Dobie R,Harvey E,Zeef L,Farrow S,Streuli C,Henderson NC,Friedman SL,Hanley NA,Piper Hanley K

更新日期：2016-08-18 00:00:00

abstract：:Synaptic function is affected in many brain diseases and disorders. Technologies for large-scale synapse assays can facilitate identification of drug leads. Here we report a 'synapse microarray' technology that enables ultra-sensitive, high-throughput and quantitative screening of synaptogenesis. Our platform enables ...

journal_title：Nature communications

authors： Shi P,Scott MA,Ghosh B,Wan D,Wissner-Gross Z,Mazitschek R,Haggarty SJ,Yanik MF

更新日期：2011-10-25 00:00:00

abstract：:Metastasis is the main cause of cancer-related death and thus understanding the molecular and cellular mechanisms underlying this process is critical. Here, our data demonstrate, contrary to established dogma, that loss of haematopoietic-derived focal adhesion kinase (FAK) is sufficient to enhance tumour metastasis. U...

journal_title：Nature communications

authors： Batista S,Maniati E,Reynolds LE,Tavora B,Lees DM,Fernandez I,Elia G,Casanovas O,Lo Celso C,Hagemann T,Hodivala-Dilke K

更新日期：2014-10-01 00:00:00

abstract：:In conventional computers, wiring between transistors is required to enable the execution of Boolean logic functions. This has resulted in processors in which billions of transistors are physically interconnected, which limits integration densities, gives rise to huge power consumption and restricts processing speeds....

journal_title：Nature communications

authors： Mahboob I,Flurin E,Nishiguchi K,Fujiwara A,Yamaguchi H

更新日期：2011-02-15 00:00:00

abstract：:It is generally assumed that cells interrogate the mechanical properties of their environment by pushing and pulling on the extracellular matrix (ECM). For instance, acto-myosin-dependent contraction forces exerted at focal adhesions (FAs) allow the cell to actively probe substrate elasticity. Here, we report that a s...

journal_title：Nature communications

authors： Baschieri F,Dayot S,Elkhatib N,Ly N,Capmany A,Schauer K,Betz T,Vignjevic DM,Poincloux R,Montagnac G

更新日期：2018-09-20 00:00:00