当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Targeted Knockdown of Overexpressed VEGFA or VEGF164 in Müller cells maintains retinal function by triggering different signaling mechanisms.

Targeted Knockdown of Overexpressed VEGFA or VEGF164 in Müller cells maintains retinal function by triggering different signaling mechanisms.

Abstract：

:Oxygen-induced retinopathy (OIR) upregulates Müller cell vascular endothelial growth factor A (VEGFA) that causes intravitreal neovascularization similar to severe retinopathy of prematurity (ROP). Safety concerns exist with anti-VEGF treatment for ROP. We evaluated long-term knockdown of Müller cell-VEGFA with short-hairpin RNAs to VEGFA or VEGF164 via subretinal lentivirus delivery (L-VEGFAshRNA, L-VEGF164shRNA) on retinal structure and function in a rat OIR model. Lectin-stained retinal flat mounts analyzed for areas of avascular/total retina (AVA) and intravitreal neovascular/total retina (IVNV) showed initial significantly reduced IVNV by L-VEGFAshRNA and L-VEGF164shRNA compared to control, luciferase-shRNA lentivirus, without late recurrence. Spectral-domain optical coherence tomography (OCT) and immunohistochemical sections (IHC) demonstrated changes in retinal layer thicknesses in L-VEGFAshRNA or L-VEGF164shRNA  compared to control. Ganzfeld electroretinograms were increased in L-VEGFAshRNA or L-VEGF164shRNA compared to control. Erythropoietin (EPO), brain-derived neurotrophic factor, glial-derived neurotrophic factor, nerve growth factor, neurotrophin-3 (NT-3) mRNAs were increased in L-VEGFAshRNA, but not L-VEGF164shRNA retinas. In cultured rat Müller cells, knockdown of VEGF upregulated NT-3 and EPO, whereas treatment with EPO activated neuroprotective signaling. Methods to reduce IVNV by selective knockdown of VEGFA, and particularly VEGF164, in Müller cells may have fewer deleterious effects than nonselective VEGFA inhibition to all cells in the retina.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Becker S,Wang H,Simmons AB,Suwanmanee T,Stoddard GJ,Kafri T,Hartnett ME

doi

10.1038/s41598-018-20278-4

subject

Has Abstract

pub_date

2018-01-31 00:00:00

pages

2003

issue

1

issn

2045-2322

pii

10.1038/s41598-018-20278-4

journal_volume

8

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Ultrafine particles and ozone perturb norepinephrine clearance rather than centrally generated sympathetic activity in humans.

    abstract：:Cardiovascular risk rapidly increased following exposure to air pollution. Changes in human autonomic regulation have been implicated based on epidemiological associations between exposure estimates and indirect autonomic nervous system measurements. We conducted a mechanistic study to test the hypothesis that, in hea...

    journal_title：Scientific reports

    pub_type： 杂志文章,随机对照试验

    doi：10.1038/s41598-019-40343-w

    authors： Heusser K,Tank J,Holz O,May M,Brinkmann J,Engeli S,Diedrich A,Framke T,Koch A,Großhennig A,Jan Danser AH,Sweep FCGJ,Schindler C,Schwarz K,Krug N,Jordan J,Hohlfeld JM

    更新日期：2019-03-06 00:00:00

  • A signal processing method for alignment-free metagenomic binning: multi-resolution genomic binary patterns.

    abstract：:Algorithms in bioinformatics use textual representations of genetic information, sequences of the characters A, T, G and C represented computationally as strings or sub-strings. Signal and related image processing methods offer a rich source of alternative descriptors as they are designed to work in the presence of no...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-38197-9

    authors： Kouchaki S,Tapinos A,Robertson DL

    更新日期：2019-02-15 00:00:00

  • Submarine Landslides Induce Massive Waves in Subsea Brine Pools.

    abstract：:Subsea hypersaline anoxic brine pools are among the most extreme habitable environments on Earth that offer clues to life on other planets. Brine is toxic to macrofauna as remotely operated vehicles commonly observe dead and preserved remains in brine pools. While brine pools are often assumed to be stable stratified ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36781-7

    authors： Sawyer DE,Mason RA,Cook AE,Portnov A

    更新日期：2019-01-15 00:00:00

  • Auranofin, at clinically achievable dose, protects mice and prevents recurrence from Clostridioides difficile infection.

    abstract：:Clostridioides difficile is the leading cause of nosocomial infections and a worldwide urgent public health threat. Without doubt, there is an urgent need for new effective anticlostridial agents due to the increasing incidence and severity of C. difficile infection (CDI). The aim of the present study is to investigat...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-64882-9

    authors： Abutaleb NS,Seleem MN

    更新日期：2020-05-07 00:00:00

  • Conical Gradient Junctions of Dendritic Viologen Arrays on Electrodes.

    abstract：:The three-dimensional construction of arrays of functional molecules on an electrode surface, such as organic semiconductors and redox-active molecules, is a considerable challenge in the fabrication of sophisticated junctions for molecular devices. In particular, well-defined organic layers with precise molecular gra...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep11122

    authors： Kawauchi T,Oguchi Y,Nagai K,Iyoda T

    更新日期：2015-06-09 00:00:00

  • Numerical analysis of wide-field optical imaging with a sub-20 nm resolution based on a meta-sandwich structure.

    abstract：:Biological research requires wide-field optical imaging techniques with resolution down to the nanometer scale to study the biological process in a sub-cell or single molecular level. To meet this requirement, wide-field structured illumination method (WFSIM) has been extensively studied. The resolution of WFSIM is de...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-01521-w

    authors： Cao S,Wang T,Yang J,Hu B,Levy U,Yu W

    更新日期：2017-05-02 00:00:00

  • Down regulation of ADAM33 as a Predictive Biomarker of Aggressive Breast Cancer.

    abstract：:Breast cancer is a heterogeneous disease with differences in its clinical, molecular and biological features. Traditionally, immunohistochemical markers together with clinicopathologic parameters are used to classify breast cancer and to predict disease outcome. Triple-negative breast cancer (TNBC) is a particular typ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep44414

    authors： Manica GC,Ribeiro CF,Oliveira MA,Pereira IT,Chequin A,Ramos EA,Klassen LM,Sebastião AP,Alvarenga LM,Zanata SM,Noronha L,Rabinovich I,Costa FF,Souza EM,Klassen G

    更新日期：2017-03-15 00:00:00

  • Network analysis suggests a potentially 'evil' alliance of opportunistic pathogens inhibited by a cooperative network in human milk bacterial communities.

    abstract：:The critical importance of human milk to infants and even human civilization has been well established. Yet our understanding of the milk microbiome has been limited to cataloguing OTUs and computation of community diversity. To the best of our knowledge, there has been no report on the bacterial interactions within t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep08275

    authors： Sam Ma Z,Guan Q,Ye C,Zhang C,Foster JA,Forney LJ

    更新日期：2015-02-05 00:00:00

  • Drug Screening of Human GBM Spheroids in Brain Cancer Chip.

    abstract：:Glioblastoma multiforme (GBM), an extremely invasive and high-grade (grade IV) glioma, is the most common and aggressive form of brain cancer. It has a poor prognosis, with a median overall survival of only 11 months in the general GBM population and 14.6 to 21 months in clinical trial participants with standard GBM t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-33641-2

    authors： Akay M,Hite J,Avci NG,Fan Y,Akay Y,Lu G,Zhu JJ

    更新日期：2018-10-18 00:00:00

  • α-SNAP is expressed in mouse ovarian granulosa cells and plays a key role in folliculogenesis and female fertility.

    abstract：:The balance between ovarian folliculogenesis and follicular atresia is critical for female fertility and is strictly regulated by a complex network of neuroendocrine and intra-ovarian signals. Despite the numerous functions executed by granulosa cells (GCs) in ovarian physiology, the role of multifunctional proteins a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-12292-9

    authors： Arcos A,de Paola M,Gianetti D,Acuña D,Velásquez ZD,Miró MP,Toro G,Hinrichsen B,Muñoz RI,Lin Y,Mardones GA,Ehrenfeld P,Rivera FJ,Michaut MA,Batiz LF

    更新日期：2017-09-18 00:00:00

  • Relationship between Lipoprotein (a) and cognitive function - Results from the Berlin Aging Study II.

    abstract：:It has been suggested that an age-related loss of cognitive function might be driven by atherosclerotic effects associated with altered lipid patterns. However, the relationship between Lipoprotein (a) [Lp(a)] and healthy cognitive aging has not yet been sufficiently investigated. For the current analysis we used the ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66783-3

    authors： Röhr F,Bucholtz N,Toepfer S,Norman K,Spira D,Steinhagen-Thiessen E,Lill CM,Bertram L,Demuth I,Buchmann N,Düzel S

    更新日期：2020-06-30 00:00:00

  • Tuned by metals: the TET peptidase activity is controlled by 3 metal binding sites.

    abstract：:TET aminopeptidases are dodecameric particles shared in the three life domains involved in various biological processes, from carbon source provider in archaea to eye-pressure regulation in humans. Each subunit contains a dinuclear metal site (M1 and M2) responsible for the enzyme catalytic activity. However, the role...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20876

    authors： Colombo M,Girard E,Franzetti B

    更新日期：2016-02-08 00:00:00

  • An RNA nanoparticle vaccine against Zika virus elicits antibody and CD8+ T cell responses in a mouse model.

    abstract：:The Zika virus (ZIKV) outbreak in the Americas and South Pacific poses a significant burden on human health because of ZIKV's neurotropic effects in the course of fetal development. Vaccine candidates against ZIKV are coming online, but immunological tools to study anti-ZIKV responses in preclinical models, particular...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-00193-w

    authors： Chahal JS,Fang T,Woodham AW,Khan OF,Ling J,Anderson DG,Ploegh HL

    更新日期：2017-03-21 00:00:00

  • Artificial photosynthesis of oxalate and oxalate-based polymer by a photovoltaic reactor.

    abstract：:A photovoltaic reactor was designed for artificial photosynthesis, based on the reactions involved in high energy hydrogen atoms, which were produced from water electrolysis. Water and CO2, under the conditions studied, were converted to oxalate (H2C2O4) and a polymer. This was the first time that the oxalates and oxa...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep03572

    authors： Nong G,Chen S,Xu Y,Huang L,Zou Q,Li S,Mo H,Zhu P,Cen W,Wang S

    更新日期：2014-01-06 00:00:00

  • Hyperspectral imaging using the single-pixel Fourier transform technique.

    abstract：:Hyperspectral imaging technology is playing an increasingly important role in the fields of food analysis, medicine and biotechnology. To improve the speed of operation and increase the light throughput in a compact equipment structure, a Fourier transform hyperspectral imaging system based on a single-pixel technique...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep45209

    authors： Jin S,Hui W,Wang Y,Huang K,Shi Q,Ying C,Liu D,Ye Q,Zhou W,Tian J

    更新日期：2017-03-24 00:00:00

  • Acute Psychological Stress Disrupts Attentional Bias to Threat-Related Stimuli.

    abstract：:The present study investigated the effect of acute stress on attentional bias to threat using behavioral and ERP methods. Sixty-two male participants were randomly assigned to a stress condition (Trier Social Stress Test) or a control condition. To examine the impact of stress-induced cortisol on attentional bias to t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14138-w

    authors： Jiang C,Buchanan TW,Yao Z,Zhang K,Wu J,Zhang L

    更新日期：2017-11-06 00:00:00

  • Evidence of Cardiovascular Calcification and Fibrosis in Pseudoxanthoma Elasticum Mouse Models Subjected to DOCA-Salt Hypertension.

    abstract：:Pseudoxanthoma Elasticum (PXE) is a rare disorder characterized by fragmentation and progressive calcification of elastic fibres in connective tissues. Although arterial hypertension (AHT) has been reported in PXE patients, its impact on pathological manifestations has as yet been unexplored. We investigated the conse...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52808-z

    authors： Omarjee L,Roy C,Leboeuf C,Favre J,Henrion D,Mahe G,Leftheriotis G,Martin L,Janin A,Kauffenstein G

    更新日期：2019-11-08 00:00:00

  • Breastfeeding and maternal cardiovascular risk factors: 1982 Pelotas Birth Cohort.

    abstract：:This study evaluated the association of breastfeeding duration with maternal metabolic cardiovascular risk factors among women who have been prospectively followed since birth in a southern Brazilian city. In the unadjusted analysis, total cholesterol was higher among women who never breastfed in relation to those who...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-49576-1

    authors： Lima NP,Bassani DG,Magalhães EIS,Barros FC,Horta BL

    更新日期：2019-09-11 00:00:00

  • Chronic kidney disease attenuates the impact of obesity on quality of life.

    abstract：:The impact of obesity on health-related quality of life (HRQoL) in chronic kidney disease (CKD) population has not been elucidated, despite the impairment of HRQoL in the obese among general population. We hypothesized that the impact of obesity on HRQoL might be confounded by impaired renal function in CKD population...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-59382-9

    authors： Suh SH,Choi HS,Kim CS,Bae EH,Ma SK,Lee DH,Kim SW

    更新日期：2020-02-11 00:00:00

  • Ultralow-phase-noise millimetre-wave signal generator assisted with an electro-optics-modulator-based optical frequency comb.

    abstract：:Low-noise millimetre-wave signals are valuable for digital sampling systems, arbitrary waveform generation for ultra-wideband communications, and coherent radar systems. However, the phase noise of widely used conventional signal generators (SGs) will increase as the millimetre-wave frequency increases. Our goal has b...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep24621

    authors： Ishizawa A,Nishikawa T,Goto T,Hitachi K,Sogawa T,Gotoh H

    更新日期：2016-05-17 00:00:00

  • Element substitution of kesterite Cu2ZnSnS4 for efficient counter electrode of dye-sensitized solar cells.

    abstract：:Development of cost-effective counter electrode (CE) materials is a key issue for practical applications of photoelectrochemical solar energy conversion. Kesterite Cu2ZnSnS4 (CZTS) has been recognized as a potential CE material, but its electrocatalytic activity is still insufficient for the recovery of I-/I3- electro...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-26770-1

    authors： Lu S,Yang H,Li F,Wang Y,Chen S,Yang G,Liu Y,Zhang X

    更新日期：2018-06-07 00:00:00

  • The Limits of Effective Degrees of Freedom in UCA based Orbital Angular Momentum Multiplexed Communications.

    abstract：:Orbital angular momentum (OAM) multiplexing technique has recently emerged and generated widespread interests since the OAM was discovered as a property of electromagnetic wave and acoustic wave. It was widely acknowledged that OAM multiplexing can achieve very high effective degrees of freedom (EDOF) and improve the ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-61329-z

    authors： Li Z,Qu F,Wei Y,Yang G,Xu W,Xu J

    更新日期：2020-03-23 00:00:00

  • Site-specific chelation therapy with EDTA-loaded albumin nanoparticles reverses arterial calcification in a rat model of chronic kidney disease.

    abstract：:Medial arterial calcification (MAC) is a common outcome in diabetes and chronic kidney disease (CKD). It occurs as linear mineral deposits along the degraded elastin lamellae and is responsible for increased aortic stiffness and subsequent cardiovascular events. Current treatments for calcification, particularly in CK...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-39639-8

    authors： Karamched SR,Nosoudi N,Moreland HE,Chowdhury A,Vyavahare NR

    更新日期：2019-02-22 00:00:00

  • Transcription factor ZNF148 is a negative regulator of human muscle differentiation.

    abstract：:Muscle differentiation is a complex process in which muscle progenitor cells undergo determination and eventually cellular fusion. This process is heavily regulated by such master transcription factors as MYOD and members of the MEF2 family. Here, we show that the transcription factor ZNF148 plays a direct role in hum...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-08267-5

    authors： Bakke J,Wright WC,Zamora AE,Ong SS,Wang YM,Hoyer JD,Brewer CT,Thomas PG,Chen T

    更新日期：2017-08-15 00:00:00

  • Aquaponics using a fish farm effluent shifts bacterial communities profile in halophytes rhizosphere and endosphere.

    abstract：:The intensification of marine aquaculture raises multiple sustainability issues, namely the handling of nutrient-rich effluents that can adversely impact ecosystems. As integrated multi-trophic aquaculture (IMTA) gains momentum, the use of halophyte plants to phytoremediate aquaculture effluents has received growing a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-66093-8

    authors： Oliveira V,Martins P,Marques B,Cleary DFR,Lillebø AI,Calado R

    更新日期：2020-06-22 00:00:00

  • In vitro exposure of simulated meat-cooking fumes to assess adverse biological effects.

    abstract：:The heterocyclic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is considered as a human carcinogenic or mutagenic compound that is produced from the co-condensation of creatinine and amino acids as meats cook at high temperatures. The cooking of meats at high temperatures produces fumes, and these fumes...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-11502-8

    authors： Poudel BK,Choi J,Park JH,Doh KO,Byeon JH

    更新日期：2017-09-07 00:00:00

  • Physical plasma-treated saline promotes an immunogenic phenotype in CT26 colon cancer cells in vitro and in vivo.

    abstract：:Metastatic colorectal cancer is the fourth most common cause of cancer death. Current options in palliation such as hyperthermic intraperitoneal chemotherapy (HIPEC) present severe side effects. Recent research efforts suggested the therapeutic use of oxidant-enriched liquid using cold physical plasma. To investigate ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37169-3

    authors： Freund E,Liedtke KR,van der Linde J,Metelmann HR,Heidecke CD,Partecke LI,Bekeschus S

    更新日期：2019-01-24 00:00:00

  • Fibrin glue as a local drug-delivery system for bacteriophage PA5.

    abstract：:Fibrin glue has been used clinically for decades in a wide variety of surgical specialties and is now being investigated as a medium for local, prolonged drug delivery. Effective local delivery of antibacterial substances is important perioperatively in patients with implanted medical devices or postoperatively for de...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-38318-4

    authors： Rubalskii E,Ruemke S,Salmoukas C,Aleshkin A,Bochkareva S,Modin E,Mashaqi B,Boyle EC,Boethig D,Rubalsky M,Zulkarneev E,Kuehn C,Haverich A

    更新日期：2019-02-14 00:00:00

  • Application of Bld-1-Embedded Elastin-Like Polypeptides in Tumor Targeting.

    abstract：:Expression of various molecules on the surface of cancer cells compared to normal cells creates a platform for the generation of various drug vehicles for targeted therapy. Multiple interactions between ligands and their receptors mediated by targeting peptide-modified polymer could enable simultaneous delivery of a d...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-21910-z

    authors： Sarangthem V,Cho EA,Yi A,Kim SK,Lee BH,Park RW

    更新日期：2018-03-01 00:00:00

  • Using Chironomus dilutus to identify toxicants and evaluate the ecotoxicity of sediments in the Haihe River Basin.

    abstract：:To effectively manage a watershed and successfully restore a river system, it is very important to assess the toxicity of sediments and identify the substances causing the toxicity. Seventy-six sediments collected in the Haihe River Basin (HRB) in China were screened for acute toxicity using Chironomus dilutus. We fou...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-01631-5

    authors： Zhu X,Shan B,Tang W,Zhang C

    更新日期：2017-05-03 00:00:00