Abstract：
:Actinorhizal plants are ecologically and economically important. Symbiosis with nitrogen-fixing bacteria allows these woody dicotyledonous plants to colonise soils under nitrogen deficiency, water-stress or other extreme conditions. However, proteins involved in xerotolerance of symbiotic microorganisms have yet to be identified. Here we characterise the polyethylene glycol (PEG)-responding desiccome from the most geographically widespread Gram-positive nitrogen-fixing plant symbiont, Frankia alni, by next-generation proteomics, taking advantage of a Q-Exactive HF tandem mass spectrometer equipped with an ultra-high-field Orbitrap analyser. A total of 2,052 proteins were detected and quantified. Under osmotic stress, PEG-grown F. alni cells increased the abundance of envelope-associated proteins like ABC transporters, mechano-sensitive ion channels and Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated (cas) components. Conjointly, dispensable pathways, like nitrogen fixation, aerobic respiration and homologous recombination, were markedly down-regulated. Molecular modelling and docking simulations suggested that the PEG is acting on Frankia partly by filling the inner part of an up-regulated osmotic-stress large conductance mechanosensitive channel.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ghedira K,Harigua-Souiai E,Ben Hamda C,Fournier P,Pujic P,Guesmi S,Guizani I,Miotello G,Armengaud J,Normand P,Sghaier Hdoi
10.1038/s41598-017-18839-0subject
Has Abstractpub_date
2018-01-15 00:00:00pages
759issue
1issn
2045-2322pii
10.1038/s41598-017-18839-0journal_volume
8pub_type
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