Abstract：
:Colistin remains one of the few antibiotics effective against multi-drug resistant (MDR) hospital pathogens, such as Klebsiella pneumoniae. Yet resistance to this last-line drug is rapidly increasing. Characterized mechanisms of colR in K. pneumoniae are largely due to chromosomal mutations in two-component regulators, although a plasmid-mediated colR mechanism has recently been uncovered. However, the effects of intrinsic colistin resistance are yet to be characterized on a whole-genome level. Here, we used a genomics-based approach to understand the mechanisms of adaptive colR acquisition in K. pneumoniae. In controlled directed-evolution experiments we observed two distinct paths to colistin resistance acquisition. Whole genome sequencing identified mutations in two colistin resistance genes: in the known colR regulator phoQ which became fixed in the population and resulted in a single amino acid change, and unstable minority variants in the recently described two-component sensor crrB. Through RNAseq and microscopy, we reveal the broad range of effects that colistin exposure has on the cell. This study is the first to use genomics to identify a population of minority variants with mutations in a colR gene in K. pneumoniae.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Cain AK,Boinett CJ,Barquist L,Dordel J,Fookes M,Mayho M,Ellington MJ,Goulding D,Pickard D,Wick RR,Holt KE,Parkhill J,Thomson NRdoi
10.1038/s41598-018-28199-ysubject
Has Abstractpub_date
2018-06-29 00:00:00pages
9868issue
1issn
2045-2322pii
10.1038/s41598-018-28199-yjournal_volume
8pub_type
杂志文章abstract：:We propose a new express method of the correlation characterization of the particles suspended in the volume of optically transparent medium. It utilizes inline digital holography technique for obtaining two images of the adjacent layers from the investigated volume with subsequent matching of the cross-correlation fu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43840
更新日期：2017-03-02 00:00:00
abstract：:Tyrosine hydroxylase (TH), the initial enzyme in the melanin pathway, catalyzes tyrosine conversion into Dopa. Although expression and regulation of TH have been shown to affect cuticle pigmentation in insects, no direct functional studies to date have focused on the specific physiological processes involving the enzy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29835
更新日期：2016-07-15 00:00:00
abstract：:Here we construct mechanically flexible and optically transparent thin film solid state supercapacitors by assembling nano-engineered carbon electrodes, prepared in porous templates, with morphology of interconnected arrays of complex shapes and porosity. The highly textured graphitic films act as electrode and curren...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00773
更新日期：2012-01-01 00:00:00
abstract：:When coming to identify new 2D materials, our intuition would suggest us to look from layered instead of 3D materials. However, since graphite can be hypothetically derived from diamond by stretching it along its [111] axis, many 3D materials can also potentially be explored as new candidates for 2D materials. Using a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24660
更新日期：2016-04-19 00:00:00
abstract：:In-vitro studies have suggested that the antiarrhythmic drug digoxin might restrain the growth of cancer cells by inhibiting Na+/K+-ATPase. We evaluated the association between cancer mortality and digoxin, sotalol and general antiarrhythmic drug use in a retrospective cohort study. The study population consists of 78...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28541-4
更新日期：2018-07-09 00:00:00
abstract：:We have engineered the electronic structure at the interface between Cu2O and ZnO nanorods (NRs) array, through adjusting the carrier concentration of Cu2O. The electrodeposition of Cu2O at pH 11 acquired the highest carrier concentration, resulting in the largest interfacial electric field between Cu2O and ZnO, which...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07882
更新日期：2015-01-20 00:00:00
abstract：:The importance of evaluating physical cues in cancer research is gradually being realized. Assessment of cancer cell physical appearance, or phenotype, may provide information on changes in cellular behavior, including migratory or communicative changes. These characteristics are intrinsically different between malign...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12083
更新日期：2015-07-13 00:00:00
abstract：:Mitochondrial DNA (mtDNA) mutations are commonly found in the skeletal muscle of patients with mitochondrial disease, inflammatory myopathies and sarcopenia. The majority of these mutations are mtDNA deletions, which accumulate to high levels in individual muscle fibres causing a respiratory defect. Most mtDNA deletio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09906
更新日期：2015-05-19 00:00:00
abstract：:The development of well-organized structures with high luminescent properties in the solid and aggregated states is of both scientific and technological interest due to their applications in nanotechnology. In this paper we described the synthesis of amphiphilic and dumbbell shaped AIE-active tetraphenylethylene (TPE)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42898
更新日期：2017-02-23 00:00:00
abstract：:Attention and saccadic eye movements are critical components of visual perception. Recent studies proposed the hypothesis of a tight coupling between saccadic adaptation (SA) and attention: SA increases the processing speed of unpredictable stimuli, while increased attentional load boosts SA. Moreover, their cortical ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70120-z
更新日期：2020-08-10 00:00:00
abstract：:In this study, ITS, ITS2, matK, rbcL and psbA-trnH in Rehmannia were successfully amplified and sequenced, but some ITS sequences need to be proofread according to ITS2 sequences. Compared with rbcL, matK and psbA-trnH, ITS and ITS2 had higher mutation rate and more information sites, and ITS2 had higher interspecific...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53752-8
更新日期：2019-11-21 00:00:00
abstract：:Tumour tracking is an advanced radiotherapy technique for precise treatment of tumours subject to organ motion. In this work, we addressed crucial aspects of dose delivery for its realisation in pencil beam scanning proton therapy, exploring the momentum acceptance and global achromaticity of a Gantry beamline to perf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71821-1
更新日期：2020-09-18 00:00:00
abstract：:High-quality broadband ultrasound transducers yield superior imaging performance in biomedical ultrasonography. However, proper design to perfectly bridge the energy between the active piezoelectric material and the target medium over the operating spectrum is still lacking. Here, we demonstrate a new anisotropic cone...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42863
更新日期：2017-02-17 00:00:00
abstract：:Most organisms are able to maintain systemic water homeostasis over a wide range of external or dietary osmolarities. The excretory system, composed of the kidneys in mammals and the Malpighian tubules and hindgut in insects, can increase water conservation and absorption to maintain systemic water homeostasis, which ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07993
更新日期：2015-01-23 00:00:00
abstract：:The hepatitis C virus (HCV) is one of the leading causes of chronic hepatitis, liver cirrhosis and hepatocellular carcinomas and infects approximately 170 million people worldwide. Although several reporter systems have been developed, many shortcomings limit their use in the assessment of HCV infections. Here, we rep...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08865
更新日期：2015-03-09 00:00:00
abstract：:Endothelial cells respond to changes in subendothelial stiffness by altering their migration and mechanics, but whether those responses are due to transcriptional reprogramming remains largely unknown. We measured traction force generation and also performed gene expression profiling for two endothelial cell types gro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54336-2
更新日期：2019-12-03 00:00:00
abstract：:Identifying early life-stage transitions limiting seagrass recruitment could improve our ability to target demographic processes most responsive to management. Here we determine the magnitude of life-stage transitions along gradients in physical disturbance limiting seedling establishment for the marine angiosperm, Po...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13833-y
更新日期：2017-11-01 00:00:00
abstract：:In recent years many monoclonal antibodies (mAb) have entered the biotherapeutics market, offering new treatments for chronic and life-threatening diseases. Protein A resin captures monoclonal antibody (mAb) effectively, but the binding capacity decays over repeated purification cycles. On an industrial scale, replaci...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30526
更新日期：2016-07-29 00:00:00
abstract：:Osteoporosis medication in fragility fracture patients is associated with better outcomes. However, limited studies have investigated whether fracture types affect outcomes among patients undergoing treatment. We performed a secondary data analysis on participants from a fracture liaison service and an osteoporosis me...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46315-4
更新日期：2019-07-12 00:00:00
abstract：:Glioblastoma (GBM) is the most aggressive brain primary malignancy. Toll-like receptor 4 (TLR4) has a dual role in cell fate, promoting cell survival or death depending on the context. Here, we analyzed TLR4 expression in different grades of astrocytoma, and observed increased expression in tumors, mainly in GBM, comp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79356-1
更新日期：2021-01-14 00:00:00
abstract：:Decline in episodic memory performance usually causes the first clinical symptoms of Alzheimer's disease. At present, Alzheimer's disease can only be diagnosed at a very late stage when neurodegeneration and cognitive impairment is already irreversible. New early disease markers are needed for earlier and more efficie...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71239-9
更新日期：2020-08-25 00:00:00
abstract：:We report the development of complete structural AlGaN-based deep-ultraviolet light-emitting diodes with an aluminum thin layer for increasing light extraction efficiency. A 217% enhancement in peak photoluminescence intensity at 294 nm is observed. Cathodoluminescence measurement demonstrates that the internal quantu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00816
更新日期：2012-01-01 00:00:00
abstract：:The concept of milk as a healthy food has opened the way for studies on milk components, from nutrients to microRNAs, molecules with broad regulatory properties present in large quantities in milk. Characterization of these components has been performed in several species, such as humans and bovine, depending on the s...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56690-7
更新日期：2019-12-30 00:00:00
abstract：:Type 2 innate lymphoid cells (ILC2) potentiate immune responses, however, their role in mediating adaptive immunity in cancer has not been assessed. Here, we report that mice genetically lacking ILC2s have significantly increased tumour growth rates and conspicuously higher frequency of circulating tumour cells (CTCs)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20608-6
更新日期：2018-02-13 00:00:00
abstract：:Repeated blood pressure (BP) measurements allow better control of hypertension. Current measurements rely on cuff-based devices. The aim of the present study was to compare BP measurements using a novel cuff-less photoplethysmography-based device to a standard sphygmomanometer device. Males and females were recruited ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73172-3
更新日期：2020-09-30 00:00:00
abstract：:In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12030
更新日期：2015-07-08 00:00:00
abstract：:Activation of G protein-gated inwardly rectifying potassium (GIRK) channels leads to a hyperpolarization of the neuron's membrane potential, providing an important component of inhibition in the brain. In addition to the canonical G protein-activation pathway, GIRK channels are activated by small molecules but less is...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04681-x
更新日期：2017-07-04 00:00:00
abstract：:We aimed to predict molecular subtypes of breast cancer using radiomics signatures extracted from synthetic mammography reconstructed from digital breast tomosynthesis (DBT). A total of 365 patients with invasive breast cancer with three different molecular subtypes (luminal A + B, luminal; HER2-positive, HER2; triple...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78681-9
更新日期：2020-12-09 00:00:00
abstract：:This retrospective cohort study examined the relationship between a cholecystectomy and the subsequent risk of peptic ulcers using a population-based database. Data for this study were retrieved from the Taiwan Longitudinal Health Insurance Database 2005. This study included 5209 patients who had undergone a cholecyst...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30702
更新日期：2016-07-29 00:00:00
abstract：:We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of imm...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00264-y
更新日期：2017-03-14 00:00:00
