Abstract：
:We developed a spongy-like porous polymer (spongy monolith) consisting of poly(ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Kubota K,Kubo T,Tanigawa T,Naito T,Otsuka Kdoi
10.1038/s41598-017-00264-ysubject
Has Abstractpub_date
2017-03-14 00:00:00pages
178issue
1issn
2045-2322pii
10.1038/s41598-017-00264-yjournal_volume
7pub_type
杂志文章abstract：:Resistance to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) has become the main clinical challenge of advanced lung cancer. This research aimed to explore the role of PARP1-mediated autophagy in the progression of TKI therapy. PARP1-mediated autophagy was evaluated in vitro by CCK-8 assay, cl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77908-z
更新日期：2020-12-01 00:00:00
abstract：:Polystyrene sulfonate (PSS), a strong polyelectrolyte, was used to prepare red photoluminescent PSS-penicillamine (PA) copper (Cu) nanoclusters (NC) aggregates, which displayed high selectivity and sensitivity to the detection of hydrogen sulfide (H2S). The size of the PSS-PA-Cu NC aggregates could be readily controll...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24882
更新日期：2016-04-26 00:00:00
abstract：:Towards exploring advanced applications of terahertz (THz) electromagnetic waves, great efforts are being applied to develop a compact and sensitive THz receiver. Here, we propose a simple coherent detection system using a single resonant tunnelling diode (RTD) oscillator through self-oscillating mixing with an RTD os...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54627-8
更新日期：2019-12-02 00:00:00
abstract：:One procedure widely used to detect the velocity of a moving object is by using the Doppler effect. This is the perceived change in frequency of a wave caused by the relative motion between the emitter and the detector, or between the detector and a reflecting target. The relative movement, in turn, generates a time-v...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02815
更新日期：2013-10-02 00:00:00
abstract：:Peritoneal macrophages play a critical role in the control of infectious and inflammatory diseases. Although recent progress on murine peritoneal macrophages has revealed multiple aspects on their origin and mechanisms involved in their maintenance in this compartment, little is known on the characteristics of human p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30787-x
更新日期：2018-08-24 00:00:00
abstract：:The temperate bamboos (tribe Arundinarieae, Poaceae) are strongly supported as monophyly in recent molecular studies, but taxonomic delineation and phylogenetic relationships within the tribe lack resolution. Here, we sampled 39 species (36 temperate bamboos and 3 outgroups) for restriction-site associated DNA sequenc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11367-x
更新日期：2017-09-14 00:00:00
abstract：:The coupling of the surface plasmon near-field into the sensing medium is key to the sensitivity of surface plasmon-based sensing devices. A low-index dielectric is necessary for the sensing medium to support a highly-penetrating surface plasmon evanescent field that extends well into the dielectric medium. The air-li...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38859-2
更新日期：2019-02-19 00:00:00
abstract：:Obstructive heart defects (OHDs) are a major health concern worldwide. The platelet-derived growth factor (PDGF) genes are known to have regulatory functions that are essential for proper heart development. In a zebrafish model, Pdgfra was further demonstrated to interact with ethanol during craniofacial development. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29160-9
更新日期：2018-07-23 00:00:00
abstract：:A long-lived reservoir of latently infected T cells prevents antiretroviral therapy from eliminating HIV-1 infection. Furthering our understanding of the dynamics of latency generation and maintenance is therefore vital to improve treatment outcome. Using mathematical models and experiments, we suggest that the death ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04130-9
更新日期：2017-06-19 00:00:00
abstract：:Phelan McDermid Syndrome (PMDS) is a genetic disorder characterized by features of Autism spectrum disorders. Similar to reports of Zn deficiency in autistic children, we have previously reported high incidence of Zn deficiency in PMDS. However, the underlying mechanisms are currently not well understood. Here, using ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45190
更新日期：2017-03-27 00:00:00
abstract：:We study the impact of light on the mammalian circadian system using the theory of phase response curves. Using a recently developed ansatz we derive a low-dimensional macroscopic model for the core circadian clock in mammals. Significantly, the variables and parameters in our model have physiological interpretations ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74002-2
更新日期：2020-11-11 00:00:00
abstract：:The spectral properties of Fano resonance generated in multilayer dielectric gratings (MDGs) are reported and numerically investigated in this paper. We examine the MDG consisting of numerous identically alternative chalcogenide glass (As2S3) and silica (SiO2) multilayers with several grating widths inscribed through ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34787-9
更新日期：2018-11-06 00:00:00
abstract：:Age-specific genetic and antigenic variations of influenza viruses have not been documented in tropical and subtropical regions. We implemented a systematic surveillance program in two tertiary hospitals in Hong Kong Island, to collect 112 A(H1N1)pdm09 and 254 A(H3N2) positive specimens from 2013 to 2014. Of these, 56...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30260
更新日期：2016-07-25 00:00:00
abstract：:The Edinburgh Postnatal Depression Scale (EPDS) is frequently used to screen for postpartum depression. However, its factor structure exhibits noticeable inconsistencies between studies. We examined the EPDS at two postpartum time points using a large dataset from outside Western countries. Participants were 91,063 mo...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1038/s41598-020-67321-x
更新日期：2020-07-15 00:00:00
abstract：:Peptides derived from the N-terminal heptad repeat (NHR) of HIV-1 gp41 can be potent inhibitors against viral entry when presented in a nonaggregating trimeric coiled-coil conformation via the introduction of exogenous trimerization motifs and intermolecular disulfide bonds. We recently discovered that crosslinking is...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32161
更新日期：2016-08-26 00:00:00
abstract：:Golden snub-nosed monkey (Rhinopithecus roxellana) is one of the most endangered primate species found in China, exhibiting multilevel society consisting of several one-male-females together with their offspring units (OMU), and all-male units (AMU). Female dispersal patterns of the species within herd have been well ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46217
更新日期：2017-05-11 00:00:00
abstract：:Colistin remains one of the few antibiotics effective against multi-drug resistant (MDR) hospital pathogens, such as Klebsiella pneumoniae. Yet resistance to this last-line drug is rapidly increasing. Characterized mechanisms of colR in K. pneumoniae are largely due to chromosomal mutations in two-component regulators...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28199-y
更新日期：2018-06-29 00:00:00
abstract：:Many cardiac pathologies involve changes in tissue structure. Conventional analysis of structural features is extremely time-consuming and subject to observer bias. The possibility to determine spatial interrelations between these features is often not fully exploited. We developed a staining protocol and an ImageJ-ba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76932-3
更新日期：2020-11-18 00:00:00
abstract：:As a human fungal pathogen, Candida albicans can cause a wide variety of disease conditions ranging from superficial to systemic infections. Many of these infections are caused by an inherent ability of the pathogen to form biofilms on medical devices resulting in high mortality. Biofilms formed by C. albicans are a c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31124
更新日期：2016-08-08 00:00:00
abstract：:The relative and combined effects of sleep apnea with diabetes mellitus (DM) on cardiovascular outcomes in patients undergoing coronary artery bypass grafting (CABG) remain unknown. In this secondary analysis of data from the SABOT study, 1007 patients were reclassified into four groups based on their sleep apnea and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78700-9
更新日期：2020-12-10 00:00:00
abstract：:Spreading rumors on the Internet has become increasingly pervasive due to the proliferation of online social media. This paper investigates how rumors are amplified by a group of users who share similar interests or views, dubbed as an echo chamber. To this end, we identify and analyze 'rumor' echo chambers, each of w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-57272-3
更新日期：2020-01-15 00:00:00
abstract：:The effect on the reproductive system and fertility of living in a space environment remains unclear. Here, we caged 12 male mice under artificial gravity (≈1 gravity) (AG) or microgravity (MG) in the International Space Station (ISS) for 35 days, and characterized the male reproductive organs (testes, epididymides, a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50128-w
更新日期：2019-09-24 00:00:00
abstract：:Estrogen-related receptor alpha (ESRRa) regulates a number of cellular processes including development of bone and muscles. However, direct evidence regarding its involvement in cartilage development remains elusive. In this report, we establish an in vivo role of Esrra in cartilage development during embryogenesis in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18011
更新日期：2015-12-10 00:00:00
abstract：:A considerable body of evidence links together mitochondrial dysfunctions, toxic action of metalloid oxyanions, and system and neurodegenerative disorders. In this study we have used the model yeast Saccharomyces cerevisiae to investigate the genetic determinants associated with tellurite resistance/sensitivity. Nitro...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30479-6
更新日期：2018-08-13 00:00:00
abstract：:Although the association between social support and postpartum depression has been previously investigated, its causal relationship remains unclear. Therefore, we examined prospectively whether social support during pregnancy affected postpartum depression. Social support and depressive symptoms were assessed by Japan...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10520
更新日期：2015-05-29 00:00:00
abstract：:Spatiotemporal tracking of tracer particles or objects of interest can reveal localized behaviors in biological and physical systems. However, existing tracking algorithms are most effective for relatively low numbers of particles that undergo displacements smaller than their typical interparticle separation distance....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23488-y
更新日期：2018-04-03 00:00:00
abstract：:Urate transporter 1 (URAT1/SLC22A12), a urate transporter gene, is a causative gene for renal hypouricemia type 1. Among several reported nonsynonymous URAT1 variants, R90H (rs121907896) and W258X (rs121907892) are frequent causative mutations for renal hypouricemia. However, no case-control study has evaluated the re...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep20148
更新日期：2016-01-29 00:00:00
abstract：:Saliva has immense potential as a diagnostic fluid for identification and monitoring of several systemic diseases. Composition of the microbiome and inflammation has been associated and reflective of oral and overall health. In addition, the relative ease of collection of saliva further strengthens large-scale diagnos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81420-3
更新日期：2021-01-29 00:00:00
abstract：:CLN3 mutations cause the fatal neurodegenerative disorder, CLN3 Batten disease. The Cln3-/- mouse model displays characteristic features of the human disease including motor deficits. When mice received acidified drinking water (pH 2.5-2.9) instead of normal tap water (pH 8.4) for several generations, the motor skills...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51488-z
更新日期：2019-10-18 00:00:00
abstract：:Sixty-six million years ago, an asteroid approximately 9 km in diameter hit the hydrocarbon- and sulfur-rich sedimentary rocks in what is now Mexico. Recent studies have shown that this impact at the Yucatan Peninsula heated the hydrocarbon and sulfur in these rocks, forming stratospheric soot and sulfate aerosols and...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14199-x
更新日期：2017-11-09 00:00:00
