Abstract:
:Arabidopsis VIRE2-INTERACTING PROTEIN2 (VIP2) was previously described as a protein with a NOT domain, and Arabidopsis vip2 mutants are recalcitrant to Agrobacterium-mediated root transformation. Here we show that VIP2 is a transcription regulator and the C-terminal NOT2 domain of VIP2 interacts with VirE2. Interestingly, AtVIP2 overexpressor lines in Arabidopsis did not show an improvement in Agrobacterium-mediated stable root transformation, but the transcriptome analysis identified 1,634 differentially expressed genes compared to wild-type. These differentially expressed genes belonged to various functional categories such as membrane proteins, circadian rhythm, signaling, response to stimulus, regulation of plant hypersensitive response, sequence-specific DNA binding transcription factor activity and transcription regulatory region binding. In addition to regulating genes involved in Agrobacterium-mediated plant transformation, AtVIP2 overexpressor line showed differential expression of genes involved in abiotic stresses. The majority of the genes involved in abscisic acid (ABA) response pathway, containing the Abscisic Acid Responsive Element (ABRE) element within their promoters, were down-regulated in AtVIP2 overexpressor lines. Consistent with this observation, AtVIP2 overexpressor lines were more susceptible to ABA and other abiotic stresses. Based on the above findings, we hypothesize that VIP2 not only plays a role in Agrobacterium-mediated plant transformation but also acts as a general transcriptional regulator in plants.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Raman V,Anand A,Vasudevan B,Morsy MR,Pant BD,Lee HK,Tang Y,Mysore KSdoi
10.1038/s41598-019-49590-3subject
Has Abstractpub_date
2019-09-18 00:00:00pages
13503issue
1issn
2045-2322pii
10.1038/s41598-019-49590-3journal_volume
9pub_type
杂志文章abstract::Chromosome segment substitution lines (CSSLs) are useful tools for precise mapping of quantitative trait loci (QTLs) and the evaluation of gene action and interaction in inter-specific crosses. In this study, a set of 90 back cross lines at BC2F8 generation derived from Swarna x Oryza nivara IRGC81832 was evaluated fo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64300-0
更新日期:2020-05-08 00:00:00
abstract::Unnatural chiral α-amino acids are widely used in fields of organic chemistry, biochemistry and medicinal chemistry, and their synthesis has attracted extensive attention. Although the asymmetric synthesis provides some efficient protocols, noble and elaborate catalysts, ligands and additives are usually required whic...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26161
更新日期:2016-05-17 00:00:00
abstract::Cortical neural activities lead to changes in the cerebral blood flow (CBF), which involves astrocytic control of cerebrovascular tone. However, the manner in which astrocytic activity specifically leads to vasodilation or vasoconstriction is difficult to determine. Here, cortical astrocytes genetically expressing a l...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11455
更新日期:2015-06-16 00:00:00
abstract::The correlation between hepatitis B virus (HBV) infection and metabolic syndrome (MetS) remains to be clarified. In this study, we explored this association in a large population in Southwest China. This was a cross-sectional study, with pooled adult health data. Multivariate logistic regression analysis, controlling ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-62609-4
更新日期:2020-04-21 00:00:00
abstract::Most large carnivore populations currently occur in heterogeneous landscapes, with source populations embedded in a matrix of human-dominated habitats. Understanding changes in distribution of endangered carnivores is critical for prioritizing and implementing conservation strategies. We examined distribution and dyna...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-39293-0
更新日期:2019-02-28 00:00:00
abstract::A child with Down syndrome, like any other child, may benefit from interacting with memory stimuli, but needs additional support and help. The use of special teaching methods, which add playfulness and use of the computer, can enhance the memory processes of these children. In this work, we present the virtual environ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60014-5
更新日期:2020-02-21 00:00:00
abstract::Gluconobacter oxydans sorbitol dehydrogenase (GoSLDH) exhibits a higher catalytic efficiency than other L-sorbose producing enzymes. During the reaction catalysed by GoSLDH, NADP+ is reduced to NADPH and D-sorbitol is oxidized to L-sorbose. However, GoSLDH activity is inhibited by the NADPH (Ki = 100 μM) formed during...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37401-0
更新日期:2019-01-28 00:00:00
abstract::We introduce a setup which realises a tunable engineered environment for experiments in circuit quantum electrodynamics. We illustrate this concept with the specific example of a quantum bit, qubit, in a high-quality-factor cavity which is capacitively coupled to another cavity including a resistor. The temperature of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01987
更新日期:2013-01-01 00:00:00
abstract::The live attenuated yellow fever (YF) vaccine is a highly effective human vaccine and induces long-term protective neutralizing antibodies directed against the viral envelope protein E. The generation of such antibodies requires the help of CD4 T cells which recognize peptides derived from proteins in virus particles ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09331-w
更新日期:2017-08-21 00:00:00
abstract::Looking for single molecule electronic devices, we have investigated the charge transport properties of individual tetra-phenylporphyrin molecules on different substrates by ultrahigh-vacuum scanning tunneling microscopy and spectroscopy and by first-principles calculations. The tetra-phenylporphyrins with a Co atom (...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08350
更新日期:2015-02-10 00:00:00
abstract::Advances in single-photon sources (SPSs) and single-photon detectors (SPDs) promise unique applications in the field of quantum information technology. In this paper, we report long-distance quantum key distribution (QKD) by using state-of-the-art devices: a quantum-dot SPS (QD SPS) emitting a photon in the telecom ba...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14383
更新日期:2015-09-25 00:00:00
abstract::Engineering of efflux pumps is a promising way to improve host's tolerance to biofuels such as medium-chain alcohols (CmOHs); however, this strategy is restricted by poor understanding of the efflux pumps engaged in extrusion of solvents. In this study, several Escherichia coli mutants of multidrug transporters were e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16505
更新日期:2015-11-13 00:00:00
abstract::Amyloid-β (Aβ) deposition is known to starts decades before the onset of clinical symptoms of Alzheimer's disease (AD), however, the detailed pathophysiological processes underlying this preclinical period are not well understood. This study aimed to investigate functional network alterations in cognitively intact eld...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06876-8
更新日期:2017-07-26 00:00:00
abstract::Quantifying multi-molecular complex assembly in specific cytoplasmic compartments is crucial to understand how cells use assembly/disassembly of these complexes to control function. Currently, biophysical methods like Fluorescence Resonance Energy Transfer and Fluorescence Correlation Spectroscopy provide quantitative...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28822
更新日期:2016-06-30 00:00:00
abstract::The network structure of biological systems provides information on the underlying processes shaping their organization and dynamics. Here we examined the structure of the network depicting protein interactions within the spliceosome, the macromolecular complex responsible for splicing in eukaryotic cells. We show the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14865
更新日期:2015-10-07 00:00:00
abstract::As a class, 'BET' inhibitors disrupt binding of bromodomain and extra-terminal motif (BET) proteins, BRD2, BRD3, BRD4 and BRDT, to acetylated histones preventing recruitment of RNA polymerase 2 to enhancers and promoters, especially super-enhancers, to inhibit gene transcription. As such, BET inhibitors may be useful ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46516-x
更新日期:2019-07-16 00:00:00
abstract::Programmed cell death 5 (PDCD5) is believed to play a crucial role in p53 activation; however, the underlying mechanism of how PDCD5 function is regulated during apoptosis remains obscure. Here, we report that the serine/threonine phosphatase PPEF-1 interacts with and dephosphorylates PDCD5 at Ser-119, which leads to ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39222
更新日期:2017-01-04 00:00:00
abstract::This paper proposes new ways to assess handwriting, a critical skill in any child's school journey. Traditionally, a pen and paper test called the BHK test (Concise Evaluation Scale for Children's Handwriting) is used to assess children's handwriting in French-speaking countries. Any child with a BHK score above a cer...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60011-8
更新日期:2020-02-21 00:00:00
abstract::Anatomically incomplete spinal cord injuries can be followed by functional recovery mediated, in part, by the formation of intraspinal detour circuits. Here, we show that adult mice recover tactile and proprioceptive function following a unilateral dorsal column lesion. We therefore investigated the basis of this reco...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67866-x
更新日期:2020-07-02 00:00:00
abstract::Purpose Global sagittal imbalance with lumbar hypo-lordosis leads to various problems in elderly populations and is often treated with long-segment fusion and osteotomy. These highly invasive procedures result in a wide range of rigid spines with a high rate of complications. Although some reports have mentioned the p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63648-7
更新日期:2020-04-21 00:00:00
abstract::In this study, dye-sensitized solar cells (DSSCs) has been assembled with poly(1-vinylpyrrolidone-co-vinyl acetate) (P(VP-co-VAc)) gel polymer electrolytes (GPEs) which have been incorporated with binary salt and an ionic liquid. The potential of this combination was studied and reported. The binary salt system GPEs w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27630
更新日期:2016-06-08 00:00:00
abstract::Islet transplantation is a novel promising strategy to cure type 1 diabetes. However, the long-term outcome is still poor, because both function and survival of the transplant decline over-time. Non-invasive imaging methods have the potential to enable monitoring of islet survival after transplantation and the effects...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15521
更新日期:2015-10-22 00:00:00
abstract::Wolfram syndrome (WS) is a recessive multisystem disorder defined by the association of diabetes mellitus and optic atrophy, reminiscent of mitochondrial diseases. The role played by mitochondria remains elusive, with contradictory results on the occurrence of mitochondrial dysfunction. We evaluated 13 recessive WS pa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61735-3
更新日期:2020-03-16 00:00:00
abstract::Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 hea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01487-9
更新日期:2017-05-02 00:00:00
abstract::The present study used 16S rRNA gene amplicon sequencing to assess the impact on salivary microbiome of different grades of dental and periodontal disease and the combination of both (hereinafter referred to as oral disease), in terms of bacterial diversity, co-occurrence network patterns and predictive models. Our sc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79875-x
更新日期:2021-01-13 00:00:00
abstract::Invasive fungal infections are a potentially life-threatening complication in immunocompromised patients. The aim of this study was to assess the efficacy and safety of posaconazole as compared with other antifungal agents for preventing invasive fungal infections in immunocompromised patients. Embase, CENTRAL, and ME...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71571-0
更新日期:2020-09-03 00:00:00
abstract::Despite the relevance of complex root microbial communities for plant health, growth and productivity, the molecular basis of these plant-microbe interactions is not well understood. Verrucomicrobia are cosmopolitans in the rhizosphere, nevertheless their adaptations and functions are enigmatic since the proportion of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65277-6
更新日期:2020-05-26 00:00:00
abstract::Phase transition is a natural phenomenon happened around our daily life, represented by the process from ice to water. While melting and solidifying at a certain temperature, a high heat of fusion is accompanied, classified as the latent heat. Phase change material (PCM) has been widely applied to store and release la...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08884
更新日期:2015-03-09 00:00:00
abstract::Colorectal cancer (CRC) is a common complex disease caused by the combination of genetic variants and environmental factors. Genome-wide association studies (GWAS) have been performed and reported some novel CRC susceptibility variants. However, the potential genetic mechanisms for newly identified CRC susceptibility ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25347
更新日期:2016-05-05 00:00:00
abstract::The modal characterization of various families of beams is a topic of current interest. We recently reported a new method for the simultaneous determination of both the azimuthal and radial mode indices for light fields possessing orbital angular momentum. The method is based upon probing the far-field diffraction pat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01422
更新日期:2013-01-01 00:00:00