Abstract:
:Antibiotics are unable to remove biofilms from surgical implants. This high antibiotic tolerance is related to bacterial persisters, a sub-population of bacteria phenotypically tolerant to antibiotics secondary to a reduced metabolic state. WLBU2 is an engineered cationic amphipathic peptide designed to maximize antimicrobial activity with minimal mammalian cell toxicity. The objective of this study was to test the ability of WLBU2 to remove Staphylococcus aureus surgical implant biofilms. WLBU2 effectively treated S. aureus biofilms formed by a variety of clinical MSSA and MRSA strains and created culture-negative implants in the in vitro biofilm model. Blocking bacterial metabolism by inhibiting oxidative phosphorylation did not affect WLBU2 killing compared to decreased killing by cefazolin. In the surgical implant infection animal model, WLBU2 decreased biofilm mass as compared to control, untreated samples. WLBU2 could rapidly eliminate implants in vitro and had sufficient efficacy in vivo with minimal systemic toxicity.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Mandell JB,Deslouches B,Montelaro RC,Shanks RMQ,Doi Y,Urish KLdoi
10.1038/s41598-017-17780-6subject
Has Abstractpub_date
2017-12-22 00:00:00pages
18098issue
1issn
2045-2322pii
10.1038/s41598-017-17780-6journal_volume
7pub_type
杂志文章abstract::The bioactive lipid sphingosine 1-phosphate (S1P) is a degradation product of sphingolipids that are particularly abundant in neurons. We have shown previously that neuronal S1P accumulation is toxic leading to ER-stress and an increase in intracellular calcium. To clarify the neuronal function of S1P, we generated br...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37064
更新日期:2016-11-24 00:00:00
abstract::Strong hydrogen bonds such as F···H···F offer new strategies to fabricate molecular architectures exhibiting novel structures and properties. Along these lines and, to potentially realize hydrogen-bond mediated superexchange interactions in a frustrated material, we synthesized [H2F]2[Ni3F6(Fpy)12][SbF6]2 (Fpy = 3-flu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23054-6
更新日期:2018-03-16 00:00:00
abstract::In recent years, texture analysis of medical images has become increasingly popular in studies investigating diagnosis, classification and treatment response assessment of cancerous disease. Despite numerous applications in oncology and medical imaging in general, there is no consensus regarding texture analysis workf...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04151-4
更新日期:2017-06-22 00:00:00
abstract::Site conditions and forest management affect dendrometric parameters of chestnut (Castanea sativa Mill.) coppices, but there is modest knowledge on the effect of stand dendrometric characters on physical and mechanical wood characteristics. The aim of this study was to verify these relationships in chestnut coppices t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80558-w
更新日期:2021-01-15 00:00:00
abstract::Bacterial outer membrane lipopolysaccharide (LPS) potently stimulates the mammalian innate immune system, and can lead to sepsis, the primary cause of death from infections. LPS is sensed by Toll-like receptor 4 (TLR4) in complex with its lipid-binding coreceptor MD-2, but subtle structural variations in LPS can profo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17997
更新日期:2015-12-09 00:00:00
abstract::Previous studies have shown that rabbit IgG antibodies against Schistosoma mansoni egg antigens (SmSEA) cross-react with allergens in natural rubber latex, peanuts and grass and tree pollens. Here we describe antigenic molecules that cross-react with rabbit anti-S. mansoni IgG antibodies in extracts of the house dust ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59892-6
更新日期:2020-02-25 00:00:00
abstract::Geographical variation of diabetic emergencies attended by prehospital emergency medical services (EMS) and the relationship between area-level social and demographic factors and risk of a diabetic emergency were examined. All cases of hypoglycaemia and hyperglycaemia attended by Ambulance Victoria between 1/01/2009 a...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1038/s41598-018-23457-5
更新日期:2018-03-23 00:00:00
abstract::Anaerobic ammonium oxidation (anammox) bacteria significantly improve the efficiency and reduce cost of nitrogen removal in wastewater treatment plants. However, their slow growth and vulnerable activity limit the application of anammox technology. In this paper, the enhancement of biotin on the nitrogen removal activ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-021-81738-y
更新日期:2021-01-21 00:00:00
abstract::The airways of individuals with cystic fibrosis (CF) are abundantly colonised by Staphylococcus aureus and Pseudomonas aeruginosa. Co-infecting hypoxic regions of static mucus within CF airways, together with decreases in pulmonary function, mucus plugging and oxygen consumption by host neutrophils gives rise to regio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42952-x
更新日期:2019-05-01 00:00:00
abstract::The experimental approach for the study of cardiometabolic disorders requires the use of animal models fed with commercial diets whose composition differs notably, even between diets used for control groups. While chow diets are usually made of agricultural by-products, purified low-fat diets (LF) contain a higher per...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59674-0
更新日期:2020-02-19 00:00:00
abstract::Procalcitonin (PCT) is a useful marker for the diagnosis of systemic inflammatory response syndrome. In addition, PCT is affected by renal function. However, few studies have investigated the relationship between PCT and the development of acute kidney injury (AKI). Hence, we investigated whether serum PCT levels at t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41291-1
更新日期:2019-03-18 00:00:00
abstract::Nanotips made of metal and semiconductor have been widely utilized in versatile applications to strengthen the electric field through lightning rod effect and localized surface plasmon resonance (LSPR) effect. Here, we present the utilization of ferroelectric nanotips to assist photoreduction of silver nanostructures ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47523-8
更新日期:2019-07-29 00:00:00
abstract::The global DNA methylation degree may be a ubiquitous and early biomarker to distinguish cancer cells from benign cells. However, its usefulness in clinical diagnosis was scarcely demonstrated, because the cancer cells isolated from patients were usually very rare. Even if 10 mL of peripheral blood was sampled from a ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11882
更新日期:2015-07-03 00:00:00
abstract::The structure and function of cellular membranes were sustained by redox-enzymes. We studied the interaction between the oxidative stress caused by excessive accumulation of ZnO-nanoparticles (ZnO-NPs) in plants and the role of redox-enzymes that can alleviate this stress. The crude callus extract from pomegranate, wh...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76664-4
更新日期:2020-11-12 00:00:00
abstract::Refractive index sensors based on surface plasmon resonance (SPR) promise to deliver high sensitivities. However, these sensitivities depend on the derivative of the monitored SPR parameters near resonance, so this dependency leads to a relatively narrow detection range for refractive index changes. Herein, we introdu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41353-4
更新日期:2019-03-26 00:00:00
abstract::Trade in ivory from extant elephant species namely Asian elephant (Elephas maximus), African savanna elephant (Loxodonta africana) and African forest elephant (Loxodonta cyclotis) is regulated internationally, while the trade in ivory from extinct species of Elephantidae, including woolly mammoth, is unregulated. This...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55094-x
更新日期:2019-12-11 00:00:00
abstract::Maritime ecosystems in Vietnam such as mangroves and mud flats are characterized by high biodiversity. However, elements of its biodiversity remain unclear and highly threatened. In this context, the assessment of rare species is a starting point to develop effective strategies for the conservation of entire ecosystem...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75200-8
更新日期:2020-10-30 00:00:00
abstract::An increase in mechanical load in the heart causes cardiac hypertrophy, either physiologically (heart development, exercise and pregnancy) or pathologically (high blood pressure and heart-valve regurgitation). Understanding cardiac hypertrophy is critical to comprehending the mechanisms of heart development and treatm...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20674
更新日期:2016-02-10 00:00:00
abstract::Vascular endothelial growth factor receptor-2 (VEGFR2) and its ligands (VEGFs) are crucial players in vasculogenesis and angiogenesis. General blocking of this signaling system with antibodies or small molecule inhibitors is an established strategy to treat cancer and age-related macular degeneration. Nevertheless, th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49646-4
更新日期:2019-09-16 00:00:00
abstract::Hexane, dichloromethane and methanol extracts of the roots of Piper sarmentosum Roxb. were screened for toxicity towards Sitophilus oryzae (L.), Rhyzopertha dominica (F.), and Plodia interpunctella (Hübner) and the hexane extract exhibited the highest mortality percentage. Bioassay-guided fractionation of the hexane e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12898-z
更新日期:2017-10-03 00:00:00
abstract::Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40176
更新日期:2017-01-10 00:00:00
abstract::The oceanic connection between the coastal variability along the southwestern African coasts and the linear equatorial dynamics at subseasonal time-scales (<120 days) is examined using a variety of model outputs, ranging from linear to general circulation models. We focus on the equatorially-forced fast and weakly dis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41847-1
更新日期:2019-03-28 00:00:00
abstract::Leishmaniases are an ensemble of diseases caused by the protozoan parasite of the genus Leishmania. Current antileishmanial treatments are limited and present main issues of toxicity and drug resistance emergence. Therefore, the generation of new inhibitors specifically directed against a leishmanial target is an attr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00848-8
更新日期:2017-04-07 00:00:00
abstract::In humans, the H+-coupled Fe2+ transporter DMT1 (SLC11A2) is essential for proper maintenance of iron homeostasis. While X-ray diffraction has recently unveiled the structure of the bacterial homologue ScaDMT as a LeuT-fold transporter, the exact mechanism of H+-cotransport has remained elusive. Here, we used a combin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-06446-y
更新日期:2017-07-28 00:00:00
abstract::Stable isotopes in water (e.g., δ2H and δ18O) are important indicators of hydrological and ecological patterns and processes. Tap water can reflect integrated features of regional hydrological processes and human activities. China is a large country with significant meteorological and geographical variations. This rep...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43653
更新日期:2017-03-02 00:00:00
abstract::Anti-cytokine therapeutic antibodies have been demonstrated to be effective in the treatment of several auto-immune disorders. However, The problems in antibody manufacture and the immunogenicity caused by multiple doses of antibodies inspire people to use auto-cytokine as immunogen to induce anti-cytokine antibodies....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14150
更新日期:2015-09-28 00:00:00
abstract::This corrects the article DOI: 10.1038/srep34617. ...
journal_title:Scientific reports
pub_type: 杂志文章,已发布勘误
doi:10.1038/srep42267
更新日期:2017-09-27 00:00:00
abstract::Many studies have now consistently reported that the phase angle of ongoing oscillatory activity (measured using EEG/MEG), at time of stimulus presentation influences detection when stimuli are near-threshold. However, studies examining whether the adjustment of the phase angle of oscillations is under top-down attent...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20423-z
更新日期:2018-02-14 00:00:00
abstract::The ability to control the spatial distribution and temporal release of a therapeutic remains a central challenge for biomedical research. Here, we report the development and optimization of a novel substrate mediated therapeutic delivery system comprising of hyaluronic acid covalently functionalized liposomes (HALNPs...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14683
更新日期:2015-10-01 00:00:00
abstract::The process, how lipids are removed from the circulation and transferred from high density lipoprotein (HDL) - a main carrier of cholesterol in the blood stream - to cells, is highly complex. HDL particles are captured from the blood stream by the scavenger receptor, class B, type I (SR-BI), the so-called HDL receptor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15949-7
更新日期:2017-11-21 00:00:00