Abstract:
:Bacterial outer membrane lipopolysaccharide (LPS) potently stimulates the mammalian innate immune system, and can lead to sepsis, the primary cause of death from infections. LPS is sensed by Toll-like receptor 4 (TLR4) in complex with its lipid-binding coreceptor MD-2, but subtle structural variations in LPS can profoundly modulate the response. To better understand the mechanism of LPS-induced stimulation and bacterial evasion, we have calculated the binding affinity to MD-2 of agonistic and antagonistic LPS variants including lipid A, lipid IVa, and synthetic antagonist Eritoran, and provide evidence that the coreceptor is a molecular switch that undergoes ligand-induced conformational changes to appropriately activate or inhibit the receptor complex. The plasticity of the coreceptor binding cavity is shown to be essential for distinguishing between ligands, whilst similar calculations for a model bacterial LPS bilayer reveal the "membrane-like" nature of the protein cavity. The ability to predict the activity of LPS variants should facilitate the rational design of TLR4 therapeutics.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Paramo T,Tomasio SM,Irvine KL,Bryant CE,Bond PJdoi
10.1038/srep17997subject
Has Abstractpub_date
2015-12-09 00:00:00pages
17997issn
2045-2322pii
srep17997journal_volume
5pub_type
杂志文章abstract::Poly(N-isopropylacrylamide) (PNIPAm) is widely used to fabricate cell sheet surfaces for cell culturing, however copolymer and interpenetrated polymer networks based on PNIPAm have been rarely explored in the context of tissue engineering. Many complex and expensive techniques have been employed to produce PNIPAm-base...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63228-9
更新日期:2020-04-09 00:00:00
abstract::Impedance, or oscillometry, measurements of the respiratory system can generate information about the function of the respiratory system not possible with traditional spirometry. There are currently several instruments on the market using different perturbations. We have compared a new respiratory oscillometry instrum...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48039-x
更新日期:2019-08-12 00:00:00
abstract::Developing an alternative method for fabricating microscale graphene patterns that overcomes the obstacles of organic contamination, linewidth resolution, and substrate damaging is paramount for applications in optoelectronics. Here we propose to pattern chemical vapor deposition grown graphene film through a stencil ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46583
更新日期:2017-04-19 00:00:00
abstract::Fundamental cell signaling mechanisms that regulate dynamic remodeling of the extracellular matrix (ECM) in mechanically loaded tissues are not yet clearly understood. Trabecular meshwork (TM) tissue in the eye is under constant mechanical stress and continuous remodeling of ECM is crucial to maintain normal aqueous h...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00845-x
更新日期:2017-04-11 00:00:00
abstract::Cardiac resynchronization therapy (CRT) threw lights on heart failure treatment, however, parts of patients showed nonresponse to CRT. Unfortunately, it lacks effective parameters to predict CRT non-response. In present study, we try to seek effective electro-echocardiographic predictors on CRT non-response. This is a...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44009
更新日期:2017-03-10 00:00:00
abstract::Graphene and its analogs in combination with metal nanopowders are among the most promising catalysts for various industry valuable processes. The newly obtained solution combustion synthesized graphene-Cu and graphene-CuNi nanocomposites were examined in heterogeneous catalysis of thermal activated CO oxidation and p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72872-0
更新日期:2020-09-30 00:00:00
abstract::Mycoplasma pneumoniae infection is conventionally diagnosed using serum antibody testing, microbial culture, and genetic testing. Recently, immunochromatography-based rapid mycoplasma antigen test kits have been developed and commercialised for rapid diagnosis of M. pneumoniae infection. However, as these kits do not ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19734-y
更新日期:2018-01-23 00:00:00
abstract::Activated sludge granules and flocs have their inherent advantages and disadvantages for wastewater treatment due to their different characteristics. So far quantitative information on their evaluation is still lacking. This work provides a quantitative and comparative evaluation on the characteristics and pollutant r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42910
更新日期:2017-02-17 00:00:00
abstract::Acute Ischemic Stroke (AIS) in the young is increasing in prevalence and the largest subtype within this cohort is cryptogenic. To curb this trend, new ways of defining cryptogenic stroke and associated risk factors are needed. We aimed to gain insights into the presence or absence of cardiovascular risk factors in ca...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79499-1
更新日期:2021-01-11 00:00:00
abstract::Early ontogenetic periods and postnatal maturation in organisms are sex-specifically sensitive to hypothalamic-pituitary-adrenal (HPA)-axis activities, related glucocorticoid secretions, and their effects on energy balance and homeostasis. Dietary polyunsaturated (PUFAs) and saturated (SFAs) fatty acids potentially pl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18978-4
更新日期:2018-01-11 00:00:00
abstract::Plant traits can influence the interactions between herbivore arthropods and their natural enemies. In these interactions generalist predators are often present, preying on herbivores and also on other arthropods in the same trophic guild. Variation in the strength of intraguild predation (IGP) may be related to habit...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14997
更新日期:2015-10-09 00:00:00
abstract::The tumour suppressor p53 is mutated in cancer, including over 96% of high-grade serous ovarian cancer (HGSOC). Mutations cause loss of wild-type p53 function due to either gain of abnormal function of mutant p53 (mutp53), or absent to low mutp53. Massively parallel sequencing (MPS) enables increased accuracy of detec...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26191
更新日期:2016-05-18 00:00:00
abstract::Human papillomavirus (HPV) infection, a primary cause of genital cancer, is also related to the increasing incidence of oropharyngeal cancer among young men. Relatively little is known about the concurrence of oral and genital infection among healthy individuals. Oral and genital swab exfoliated cells were collected s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15637
更新日期:2015-10-27 00:00:00
abstract::Common reference values of arterial stiffness indices could be effective screening tool in detecting vascular phenotypes at risk. However, populations of the same ethnicity may differ in vascular phenotype due to different environmental pressure. We examined applicability of normative equations for central augmentatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27016
更新日期:2016-05-27 00:00:00
abstract::Oxidative stress and mitochondrial dysfunction have been associated with metabolic and age-related diseases. Thus, the prevention of mitochondrial oxidative damage is nowadays a recognized pharmacological strategy to delay disease progression. Epidemiological studies suggested an association between the consumption of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-07272-y
更新日期:2017-07-28 00:00:00
abstract::This study aimed to determine the role of magnetic resonance imaging (MRI) in diagnosing and describing the characteristics of fistula-in-ano, and the agreement between MRI and operative findings. We conducted a retrospective study in 367 patients with fistula-in-ano who were diagnosed and had an operation at the Univ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54441-2
更新日期:2019-11-29 00:00:00
abstract::Lactose intolerance is a major concern driving the growth of lactose-free foods including lactose-free infant formula. It is unknown what the metabolic consequence is of consumption of a formula where lactose has been replaced with corn syrup solids (CSS). Here, a randomized double-blinded intervention study was condu...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-017-03975-4
更新日期:2017-06-16 00:00:00
abstract::The relative effects of internal fixation strategies for intertrochanteric fracture after operation remain uncertain. We conducted a systematic review and meta-analysis of randomized controlled trials (RCTs) to address this important issue. We searched PubMed, EMBASE and CENTRAL for RCTs that compared different intern...
journal_title:Scientific reports
pub_type: 杂志文章,meta分析,评审
doi:10.1038/srep18195
更新日期:2015-12-11 00:00:00
abstract::Active deep-sea hydrothermal vents harbor abundant thermophilic and hyperthermophilic microorganisms. However, microbial communities in inactive hydrothermal vents have not been well documented. Here, we investigated bacterial and archaeal communities in the two deep-sea sediments (named as TVG4 and TVG11) collected f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25982
更新日期:2016-05-12 00:00:00
abstract::Posttraumatic growth (PTG) is defined as positive psychological change in the wake of highly challenging circumstances. Healthcare workers in particular are more vulnerable to stressors and trauma than the general population. The current study examined the use and effectiveness of a novel positive psychological interv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39189
更新日期:2016-12-20 00:00:00
abstract::A comprehensive understanding of the charge/discharge behaviour of high-capacity anode active materials, e.g., Si and Li, is essential for the design and development of next-generation high-performance Li-based batteries. Here, we demonstrate the in situ scanning electron microscopy (in situ SEM) of Si anodes in a con...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36153
更新日期:2016-10-26 00:00:00
abstract::The influence of an auroral substorm on the total electron content (TEC) jumps and cycle slips on Global Positioning System (GPS) at high-latitudes is studied. For the first time, optical data from the all-sky imager, as well as interplanetary magnetic field and magnetometer data are used to complete the analysis of t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63422-9
更新日期:2020-04-14 00:00:00
abstract::The voluntary control of movement is often tested by using the countermanding, or stop-signal task that sporadically requires the suppression of a movement in response to an incoming stop-signal. Neurophysiological recordings in monkeys engaged in the countermanding task have shown that dorsal premotor cortex (PMd) is...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32669-8
更新日期:2018-09-24 00:00:00
abstract::Pancreatic ductal adenocarcinoma (PDAC) patients have poor prognosis and poor response to treatment. This is largely due to PDAC being associated with a dense and active stroma and tumor fibrosis (desmoplasia). Desmoplasia is characterized by excessive degradation and formation of the extracellular matrix (ECM) genera...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1038/s41598-019-56268-3
更新日期:2019-12-24 00:00:00
abstract::Advances in lab-on-a-chip (LOC) devices have led to significant improvements in the on-chip manipulation, separation, sorting, and isolation of particles and cells. Among various LOC-based approaches such as inertia-based methods, acoustophoresis, and magnetophoresis, the planar-slanted-electrode dielectrophoresis (DE...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-68699-4
更新日期:2020-07-16 00:00:00
abstract::The extensive reliance on insecticides to control Aedes aegypti mosquitoes and disrupt transmission of dengue, chikungunya and Zika has fueled the emergence of widespread resistance to insecticides. Mismatch between the frequency of pyrethroid resistance in mosquitoes and the occurrence of pyrethroid-based insecticide...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30968-8
更新日期:2018-08-22 00:00:00
abstract::To evaluate the association between vision-related quality of life (QoL), physical inactivity, and locomotor dysfunction in subjects with visual impairment.This cross-sectional study included 215 visually impaired subjects recruited from six ophthalmology departments in Japan. The physical inactivity and locomotor dys...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30599-z
更新日期:2018-08-13 00:00:00
abstract::The concept of novel responsive materials with a displacement conversion capability was further developed through the design of new machine-augmented composites (MACs). Embedded converter machines and MACs with improved geometry were designed and fabricated by multi-material 3D printing. This technique proved to be ve...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03783
更新日期:2014-01-21 00:00:00
abstract::Rotaviruses (RVs) are the most common cause of severe diarrheal disease. To date two rotavirus oral vaccines are licensed: Rotarix and Rotateq. Our aim was to contribute to the post-marketing evaluation of these vaccines safety profile. We collected all RV vaccines-related reports of Adverse Events Following Immunizat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70653-3
更新日期:2020-08-12 00:00:00
abstract::Limbal stem cell deficiency can be treated with transplantation of cultured human limbal epithelial cells (LEC). It can be advantageous to produce LEC in centralized labs and thereafter ship them to eye clinics. The present study used transport simulations of LEC to determine if vigorous shaking during transport alter...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28553-0
更新日期:2018-07-12 00:00:00