Clinical Evaluation of the Immunochromatographic System Using Silver Amplification for the Rapid Detection of Mycoplasma pneumoniae.

Abstract:

:Mycoplasma pneumoniae infection is conventionally diagnosed using serum antibody testing, microbial culture, and genetic testing. Recently, immunochromatography-based rapid mycoplasma antigen test kits have been developed and commercialised for rapid diagnosis of M. pneumoniae infection. However, as these kits do not provide sufficient sensitivity and specificity, a rapid test kit with improved accuracy is desired. The present prospective study evaluated a rapid M. pneumoniae diagnostic system utilizing a newly developed silver amplification immunochromatography (SAI) system. We performed dilution sensitivity test and the prospective clinical study evaluating the SAI system. The subjects of the clinical study included both children and adults. All patients suspected to have mycoplasma pneumonia (169 patients) were sequentially enrolled. Twelve patients did not agree to participate and 157 patients were enrolled in the study. The results demonstrate excellent performance of this system with 90.4% sensitivity and 100.0% specificity compared with real-time polymerase chain reaction. When compared with loop-mediated isothermal amplification (LAMP) methods, the results also demonstrate a high performance of this system with 93.0% sensitivity and 100.0% specificity. The SAI system uses a dedicated device for automatic analysis and reading, making it highly objective, and requires less human power, supporting its usefulness in clinical settings.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Namkoong H,Yamazaki M,Ishizaki M,Endo I,Harada N,Aramaki M,Tanaka Y,Kaburagi S,Ichikawa M,Ohata T,Sakaguchi S,Saito F,Nakao A,Yuki H,Mitamura K

doi

10.1038/s41598-018-19734-y

subject

Has Abstract

pub_date

2018-01-23 00:00:00

pages

1430

issue

1

issn

2045-2322

pii

10.1038/s41598-018-19734-y

journal_volume

8

pub_type

杂志文章
  • Author Correction: Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-35437-w

    authors: Li R,Tun HM,Jahan M,Zhang Z,Kumar A,Dilantha Fernando WG,Farenhorst A,Khafipour E

    更新日期:2018-11-22 00:00:00

  • Carbon nanotube intramolecular p-i-n junction diodes with symmetric and asymmetric contacts.

    abstract::A p-i-n junction diode based on the selectively doped single-walled carbon nanotube (SWCNT) had been investigated, in which two opposite ends of individual SWCNT channel were doped into the p- and n-type SWCNT respectively while the middle segment of SWCNT was kept as the intrinsic. The symmetric and asymmetric contac...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22203

    authors: Chen C,Liao C,Wei L,Zhong H,He R,Liu Q,Liu X,Lai Y,Song C,Jin T,Zhang Y

    更新日期:2016-02-26 00:00:00

  • The steady enhancement of the Australian Summer Monsoon in the last 200 years.

    abstract::A new bicentennial series of the Australian monsoon strength based on historical wind observations has allowed for the assessment of the variability of this system since the early 19th century. Our series covers a period in which the scarcity of meteorological observations in the area had precluded the evaluation of l...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16414-1

    authors: Gallego D,García-Herrera R,Peña-Ortiz C,Ribera P

    更新日期:2017-11-23 00:00:00

  • Ethoxyquin provides neuroprotection against cisplatin-induced neurotoxicity.

    abstract::Ethoxyquin was recently identified as a neuroprotective compound against toxic neuropathies and efficacy was demonstrated against paclitaxel-induced neurotoxicity in vivo. In this study we examined the efficacy of ethoxyquin in preventing neurotoxicity of cisplatin in rodent models of chemotherapy-induced peripheral n...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28861

    authors: Zhu J,Carozzi VA,Reed N,Mi R,Marmiroli P,Cavaletti G,Hoke A

    更新日期:2016-06-28 00:00:00

  • Fibronectin synthesis, but not α-smooth muscle expression, is regulated by periostin in gingival healing through FAK/JNK signaling.

    abstract::During skin healing, periostin facilitates myofibroblast differentiation through a β1 integrin/FAK dependent mechanism and continued expression is associated with scarring. In contrast to skin, gingival tissue does not typically scar upon injury, but the role of periostin in gingival healing has never been investigate...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35805-6

    authors: Kim SS,Nikoloudaki GE,Michelsons S,Creber K,Hamilton DW

    更新日期:2019-02-25 00:00:00

  • Teleporting photonic qudits using multimode quantum scissors.

    abstract::Teleportation plays an important role in the communication of quantum information between the nodes of a quantum network and is viewed as an essential ingredient for long-distance Quantum Cryptography. We describe a method to teleport the quantum information carried by a photon in a superposition of a number d of ligh...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03548

    authors: Goyal SK,Konrad T

    更新日期:2013-12-19 00:00:00

  • Elongating maize root: zone-specific combinations of polysaccharides from type I and type II primary cell walls.

    abstract::The dynamics of cell wall polysaccharides may modulate the cell wall mechanics and thus control the expansion growth of plant cells. The unique composition of type II primary cell wall characteristic of grasses suggests that they employ specific mechanisms for cell enlargement. We characterized the transcriptomes in f...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-67782-0

    authors: Kozlova LV,Nazipova AR,Gorshkov OV,Petrova AA,Gorshkova TA

    更新日期:2020-07-02 00:00:00

  • Ravens parallel great apes in physical and social cognitive skills.

    abstract::Human children show unique cognitive skills for dealing with the social world but their cognitive performance is paralleled by great apes in many tasks dealing with the physical world. Recent studies suggested that members of a songbird family-corvids-also evolved complex cognitive skills but a detailed understanding ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77060-8

    authors: Pika S,Sima MJ,Blum CR,Herrmann E,Mundry R

    更新日期:2020-12-10 00:00:00

  • Discovery of prenylated flavonoids with dual activity against influenza virus and Streptococcus pneumoniae.

    abstract::Influenza virus neuraminidase (NA) is the primary target for influenza therapeutics. Severe complications are often related to secondary pneumonia caused by Streptococcus pneumoniae (pneumococci), which also express NAs. Recently, a NA-mediated lethal synergism between influenza A viruses and pneumococci was described...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27156

    authors: Grienke U,Richter M,Walther E,Hoffmann A,Kirchmair J,Makarov V,Nietzsche S,Schmidtke M,Rollinger JM

    更新日期:2016-06-03 00:00:00

  • Chronic post-stroke aphasia severity is determined by fragmentation of residual white matter networks.

    abstract::Many stroke survivors with aphasia in the acute period experience spontaneous recovery within the first six months after the stroke. However, approximately 30-40% sustain permanent aphasia and the factors determining incomplete recovery are unclear. Suboptimal recovery may be influenced by disruption of areas seemingl...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07607-9

    authors: Marebwa BK,Fridriksson J,Yourganov G,Feenaughty L,Rorden C,Bonilha L

    更新日期:2017-08-15 00:00:00

  • Comb-rooted multi-channel synthesis of ultra-narrow optical frequencies of few Hz linewidth.

    abstract::We report a multi-channel optical frequency synthesizer developed to generate extremely stable continuous-wave lasers directly out of the optical comb of an Er-doped fiber oscillator. Being stabilized to a high-finesse cavity with a fractional frequency stability of 3.8 × 10-15 at 0.1 s, the comb-rooted synthesizer pr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44122-5

    authors: Jang H,Kim BS,Chun BJ,Kang HJ,Jang YS,Kim YW,Kim YJ,Kim SW

    更新日期:2019-05-21 00:00:00

  • The novel method to reduce the silica content in lignin recovered from black liquor originating from rice straw.

    abstract::Difficulties in the production of lignin from rice straw because of high silica content in the recovered lignin reduce its recovery yield and applications as bio-fuel and aromatic chemicals. Therefore, the objective of this study is to develop a novel method to reduce the silica content in lignin from rice straw more ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77867-5

    authors: Do NH,Pham HH,Le TM,Lauwaert J,Diels L,Verberckmoes A,Do NHN,Tran VT,Le PK

    更新日期:2020-12-04 00:00:00

  • A novel method for calculating the dynamic capillary force and correcting the pressure error in micro-tube experiment.

    abstract::Micro-tube experiment has been implemented to understand the mechanisms of governing microcosmic fluid percolation and is extensively used in both fields of micro electromechanical engineering and petroleum engineering. The measured pressure difference across the microtube is not equal to the actual pressure differenc...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16870-9

    authors: Wang S,Liu P,Zhao H,Zhang Y

    更新日期:2017-11-29 00:00:00

  • Myocardial Injury After Ischemia/Reperfusion Is Attenuated By Pharmacological Galectin-3 Inhibition.

    abstract::Although optimal therapy for myocardial infarction includes reperfusion to restore blood flow to the ischemic region, ischemia/reperfusion (IR) also initiates an inflammatory response likely contributing to adverse left ventricular (LV) extracellular matrix (ECM) remodeling. Galectin-3 (Gal-3), a β-galactoside-binding...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-46119-6

    authors: Ibarrola J,Matilla L,Martínez-Martínez E,Gueret A,Fernández-Celis A,Henry JP,Nicol L,Jaisser F,Mulder P,Ouvrard-Pascaud A,López-Andrés N

    更新日期:2019-07-03 00:00:00

  • Glacier ablation and temperature indexed melt models in the Nepalese Himalaya.

    abstract::Temperature index (TI) models are convenient for modelling glacier ablation since they require only a few input variables and rely on simple empirical relations. The approach is generally assumed to be reliable at lower elevations (below 3500 m above sea level, a.s.l) where air temperature (Ta) relates well to the ene...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41657-5

    authors: Litt M,Shea J,Wagnon P,Steiner J,Koch I,Stigter E,Immerzeel W

    更新日期:2019-03-27 00:00:00

  • Impact of laterality and mucinous histology on relapse-free and overall survival in a registry-based colon cancer series.

    abstract::Recent data suggest that tumor laterality and mucinous histology may be clinically relevant. We investigated how both variables impact on the prognosis and the response to therapies in a large population-based cohort of cancer patients. Incidence data, clinical and pathological features, and outcome were systematicall...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40096-6

    authors: Negri F,De Giorgi A,Gilli A,Azzoni C,Bottarelli L,Gnetti L,Goldoni M,Manotti L,Sgargi P,Michiara M,Leonardi F,Rindi G,Cascinu S,Silini EM

    更新日期:2019-03-06 00:00:00

  • The cellular heat shock response monitored by chemical exchange saturation transfer MRI.

    abstract::CEST-MRI of the rNOE signal has been demonstrated in vitro to be closely linked to the protein conformational state. As the detectability of denaturation and aggregation processes on a physiologically relevant scale in living organisms has yet to be verified, the aim of this study was to perform heat-shock experiments...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68022-1

    authors: Kleimaier D,Goerke S,Nies C,Zaiss M,Kunz P,Bachert P,Ladd ME,Gottwald E,Schad LR

    更新日期:2020-07-06 00:00:00

  • Inhibition of Influenza A Virus Infection by Fucoidan Targeting Viral Neuraminidase and Cellular EGFR Pathway.

    abstract::Development of novel anti-influenza A virus (IAV) drugs with high efficiency and low toxicity is critical for preparedness against influenza outbreaks. Herein, we investigated the anti-IAV activities and mechanisms of fucoidan in vitro and in vivo. The results showed that a fucoidan KW derived from brown algae Kjellma...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40760

    authors: Wang W,Wu J,Zhang X,Hao C,Zhao X,Jiao G,Shan X,Tai W,Yu G

    更新日期:2017-01-17 00:00:00

  • Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34.

    abstract::Modern DNA sequencing capabilities have led to the discovery of a large number of new bacteriophage genomes, which are a rich source of novel proteins with an unidentified biological role. The genome of Enterobacter cancerogenus bacteriophage Enc34 contains several proteins of unknown function that are nevertheless co...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15774-y

    authors: Cernooka E,Rumnieks J,Tars K,Kazaks A

    更新日期:2017-11-14 00:00:00

  • Three-dimensional Cardiomyocytes Structure Revealed By Diffusion Tensor Imaging and Its Validation Using a Tissue-Clearing Technique.

    abstract::We characterized the microstructural response of the myocardium to cardiovascular disease using diffusion tensor imaging (DTI) and performed histological validation by intact, un-sectioned, three-dimensional (3D) histology using a tissue-clearing technique. The approach was validated in normal (n = 7) and ischemic (n ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-24622-6

    authors: Lee SE,Nguyen C,Yoon J,Chang HJ,Kim S,Kim CH,Li D

    更新日期:2018-04-27 00:00:00

  • First report of community dynamics of arbuscular mycorrhizal fungi in radiocesium degradation lands after the Fukushima-Daiichi Nuclear disaster in Japan.

    abstract::Arbuscular mycorrhizal fungi (AMF) can be beneficial for improving restoration of radioactive-cesium (137Cs)-contaminated soils through soil remediation. However, there has been no information on species diversity and the composition of AMF communities in 137Cs-contaminated soil after the Fukushima-Daiichi Nuclear Pow...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44665-7

    authors: Higo M,Kang DJ,Isobe K

    更新日期:2019-06-03 00:00:00

  • Deletion of biosynthetic genes, specific SNP patterns and differences in transcript accumulation cause variation in hydroxynitrile glucoside content in barley cultivars.

    abstract::Barley (Hordeum vulgare L.) produces five leucine-derived hydroxynitrile glucosides, potentially involved in alleviating pathogen and environmental stresses. These compounds include the cyanogenic glucoside epiheterodendrin. The biosynthetic genes are clustered. Total hydroxynitrile glucoside contents were previously ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-41884-w

    authors: Ehlert M,Jagd LM,Braumann I,Dockter C,Crocoll C,Motawia MS,Møller BL,Lyngkjær MF

    更新日期:2019-04-05 00:00:00

  • A spheroid toxicity assay using magnetic 3D bioprinting and real-time mobile device-based imaging.

    abstract::An ongoing challenge in biomedical research is the search for simple, yet robust assays using 3D cell cultures for toxicity screening. This study addresses that challenge with a novel spheroid assay, wherein spheroids, formed by magnetic 3D bioprinting, contract immediately as cells rearrange and compact the spheroid ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep13987

    authors: Tseng H,Gage JA,Shen T,Haisler WL,Neeley SK,Shiao S,Chen J,Desai PK,Liao A,Hebel C,Raphael RM,Becker JL,Souza GR

    更新日期:2015-09-14 00:00:00

  • Ecosystem regime change inferred from the distribution of trace metals in Lake Erie sediments.

    abstract::Many freshwater and coastal marine ecosystems across the world may have undergone an ecosystem regime change due to a combination of rising anthropogenic disturbances and regional climate change. Such a change in aquatic ecosystems is commonly seen as shifts in algal species. But considerably less detail is known abou...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep07265

    authors: Yuan F,Depew R,Soltis-Muth C

    更新日期:2014-12-01 00:00:00

  • New Insight into AuNP Applications in Tumour Treatment and Cosmetics through Wavy Annuli at the Nanoscale.

    abstract::The purpose of this study is to probe the peristaltic propulsion of a non-Newtonian fluid model with suspended gold nanoparticles. The base fluid is considered to simulate blood using the Carreau fluid model. We model a small annulus as a tube with a peristaltic wave containing a clot propagating towards the tube wall...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36459-0

    authors: Abdelsalam SI,Bhatti MM

    更新日期:2019-01-22 00:00:00

  • An efficient full-length cDNA amplification strategy based on bioinformatics technology and multiplexed PCR methods.

    abstract::A novel strategy for amplification full-length cDNA and promoter sequences has been developed using bioinformatics technology and multiplexed PCR methods in this study. The amplification of 3' ends of cDNA is performed according to the modified classic 3' RACE techniques, therein the more efficient and effective oligo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19420

    authors: Chen N,Wang WM,Wang HL

    更新日期:2016-01-13 00:00:00

  • Deterministic Current Induced Magnetic Switching Without External Field using Giant Spin Hall Effect of β-W.

    abstract::Giant spin Hall effect (GSHE) has received significant attention for its potential in future spintronic applications. Spin current via GSHE-based thin films provides an effective and promising means to manipulate magnetization. However, an external in-plane magnetic field is required to consistently switch the perpend...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26586-z

    authors: Chen W,Qian L,Xiao G

    更新日期:2018-05-25 00:00:00

  • In silico/In vivo analysis of high-risk papillomavirus L1 and L2 conserved sequences for development of cross-subtype prophylactic vaccine.

    abstract::Human papillomavirus (HPV) is the most common sexually transmitted infection in the world and the main cause of cervical cancer. Nowadays, the virus-like particles (VLPs) based on L1 proteins have been considered as the best candidate for vaccine development against HPV infections. Two commercial HPV (Gardasil and Cer...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-51679-8

    authors: Namvar A,Bolhassani A,Javadi G,Noormohammadi Z

    更新日期:2019-10-23 00:00:00

  • Analysis of the metabolome of Anopheles gambiae mosquito after exposure to Mycobacterium ulcerans.

    abstract::Infection with Mycobacterium ulcerans causes Buruli Ulcer, a neglected tropical disease. Mosquito vectors are suspected to participate in the transmission and environmental maintenance of the bacterium. However, mechanisms and consequences of mosquito contamination by M. ulcerans are not well understood. We evaluated ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep09242

    authors: Hoxmeier JC,Thompson BD,Broeckling CD,Small P,Foy BD,Prenni J,Dobos KM

    更新日期:2015-03-18 00:00:00

  • Elevation of autophagy markers in Sjögren syndrome dry eye.

    abstract::Autophagy is known to be implicated in the pathogenesis of Sjögren syndrome (SS), but evidences are limited. We aimed to examine the levels of autophagy markers in tear film and conjunctival epithelial cells from SS dry eye patients, and analyze their correlations with clinical features. Patients with SS dry eye exhib...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-17128-0

    authors: Byun YS,Lee HJ,Shin S,Chung SH

    更新日期:2017-12-08 00:00:00