Clinical Evaluation of the Immunochromatographic System Using Silver Amplification for the Rapid Detection of Mycoplasma pneumoniae.

Abstract:

:Mycoplasma pneumoniae infection is conventionally diagnosed using serum antibody testing, microbial culture, and genetic testing. Recently, immunochromatography-based rapid mycoplasma antigen test kits have been developed and commercialised for rapid diagnosis of M. pneumoniae infection. However, as these kits do not provide sufficient sensitivity and specificity, a rapid test kit with improved accuracy is desired. The present prospective study evaluated a rapid M. pneumoniae diagnostic system utilizing a newly developed silver amplification immunochromatography (SAI) system. We performed dilution sensitivity test and the prospective clinical study evaluating the SAI system. The subjects of the clinical study included both children and adults. All patients suspected to have mycoplasma pneumonia (169 patients) were sequentially enrolled. Twelve patients did not agree to participate and 157 patients were enrolled in the study. The results demonstrate excellent performance of this system with 90.4% sensitivity and 100.0% specificity compared with real-time polymerase chain reaction. When compared with loop-mediated isothermal amplification (LAMP) methods, the results also demonstrate a high performance of this system with 93.0% sensitivity and 100.0% specificity. The SAI system uses a dedicated device for automatic analysis and reading, making it highly objective, and requires less human power, supporting its usefulness in clinical settings.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Namkoong H,Yamazaki M,Ishizaki M,Endo I,Harada N,Aramaki M,Tanaka Y,Kaburagi S,Ichikawa M,Ohata T,Sakaguchi S,Saito F,Nakao A,Yuki H,Mitamura K

doi

10.1038/s41598-018-19734-y

subject

Has Abstract

pub_date

2018-01-23 00:00:00

pages

1430

issue

1

issn

2045-2322

pii

10.1038/s41598-018-19734-y

journal_volume

8

pub_type

杂志文章
  • Magnetic properties of the complex concentrated alloy system CoFeNi0.5Cr0.5Alx.

    abstract::We study the change in magnetisation with paramagnetic Al addition in the CoFeNi0.5Cr0.5-Alx (x: 0, 0.5, 1, and 1.5) complex concentrated alloy. The compositions were developed utilising the Mulliken electronegativity and d-electron/atom ratio. Spherical FeCr rich nanoprecipitates are observed for X: 1.0 and 1.5 in an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-71463-3

    authors: Morley NA,Lim B,Xi J,Quintana-Nedelcos A,Leong Z

    更新日期:2020-09-02 00:00:00

  • Generation of novel patient-derived CIC- DUX4 sarcoma xenografts and cell lines.

    abstract::CIC-DUX4 sarcoma (CDS) is a group of rare, mesenchymal, small round cell tumours that harbour the unique CIC-DUX4 translocation, which causes aberrant gene expression. CDS exhibits an aggressive course and poor clinical outcome, thus novel therapeutic approaches are needed for CDS treatment. Although patient-derived c...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-04967-0

    authors: Oyama R,Takahashi M,Yoshida A,Sakumoto M,Takai Y,Kito F,Shiozawa K,Qiao Z,Arai Y,Shibata T,Araki Y,Endo M,Kawai A,Kondo T

    更新日期:2017-07-05 00:00:00

  • Toll like receptor7 polymorphisms in relation to disease susceptibility and progression in Chinese patients with chronic HBV infection.

    abstract::Toll-like receptors (TLRs) play a key role in innate and adaptive immunity, protecting the host from viral pathogens. We studied the effect of TLR7 polymorphisms on disease susceptibility and progression of chronic hepatitis B (CHB) infection in Chinese adults. Blood samples were taken from 612 patients with confirmed...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12698-5

    authors: Zhu J,Zhang T,Cao L,Li A,Zheng K,Zhang N,Su B,Chen Z,Chen N,Wu H,He Q

    更新日期:2017-09-29 00:00:00

  • The cellular and molecular determinants of emphysematous destruction in COPD.

    abstract::The introduction of microCT has made it possible to show that the terminal bronchioles are narrowed and destroyed before the onset of emphysematous destruction in COPD. This report extends those observations to the cellular and molecular level in the centrilobular phenotype of emphysematous destruction in lungs donate...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10126-2

    authors: Suzuki M,Sze MA,Campbell JD,Brothers JF 2nd,Lenburg ME,McDonough JE,Elliott WM,Cooper JD,Spira A,Hogg JC

    更新日期:2017-08-25 00:00:00

  • Oncologist-led BRCA 'mainstreaming' in the ovarian cancer clinic: A study of 255 patients and its impact on their management.

    abstract::Although guidelines recommend BRCA testing for all women with non-mucinous epithelial ovarian cancer, there is significant variability in access to testing across the UK. A germline BRCA mutation (BRCAm) in ovarian cancer patients provides prognostic and predictive information and influences clinical management, such ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60149-5

    authors: Rumford M,Lythgoe M,McNeish I,Gabra H,Tookman L,Rahman N,George A,Krell J

    更新日期:2020-02-25 00:00:00

  • The different effects of twin boundary and grain boundary on reducing tension-compression yield asymmetry of Mg alloys.

    abstract::In the present study, a coarse grained AZ31 plate was refined by twin boundaries (TBs) and grain boundaries (GBs), respectively. A comparative study about the different effects of grain refinements by GBs and by TBs on tension-compression yield asymmetry was performed. Our results show that both the refinements by GBs...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29283

    authors: Yu H,Xin Y,Chapuis A,Huang X,Xin R,Liu Q

    更新日期:2016-07-04 00:00:00

  • Publisher Correction: Alteration in Fluidity of Cell Plasma Membrane in Huntington Disease Revealed by Spectral Phasor Analysis.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-24031-9

    authors: Sameni S,Malacrida L,Tan Z,Digman MA

    更新日期:2018-04-11 00:00:00

  • Short-term CFTR inhibition reduces islet area in C57BL/6 mice.

    abstract::Cystic fibrosis-related diabetes (CFRD) worsens CF lung disease leading to early mortality. Loss of beta cell area, even without overt diabetes or pancreatitis is consistently observed. We investigated whether short-term CFTR inhibition was sufficient to impact islet morphology and function in otherwise healthy mice. ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47745-w

    authors: Khan D,Kelsey R,Maheshwari RR,Stone VM,Hasib A,Manderson Koivula FN,Watson A,Harkin S,Irwin N,Shaw JA,McClenaghan NH,Venglovecz V,Ébert A,Flodström-Tullberg M,White MG,Kelly C

    更新日期:2019-08-02 00:00:00

  • Meter enhances the subcortical processing of speech sounds at a strong beat.

    abstract::The temporal structure of sound such as in music and speech increases the efficiency of auditory processing by providing listeners with a predictable context. Musical meter is a good example of a sound structure that is temporally organized in a hierarchical manner, with recent studies showing that meter optimizes neu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72714-z

    authors: Moon IJ,Kang S,Boichenko N,Hong SH,Lee KM

    更新日期:2020-09-29 00:00:00

  • A crystal structure-guided rational design switching non-carbohydrate inhibitors' specificity between two β-GlcNAcase homologs.

    abstract::Selective inhibition of function-specific β-GlcNAcase has great potential in terms of drug design and biological research. The symmetrical bis-naphthalimide M-31850 was previously obtained by screening for specificity against human glycoconjugate-lytic β-GlcNAcase. Using protein-ligand co-crystallization and molecular...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06188

    authors: Liu T,Guo P,Zhou Y,Wang J,Chen L,Yang H,Qian X,Yang Q

    更新日期:2014-08-26 00:00:00

  • Implicit emotion regulation deficits in individuals with high schizotypal traits: an ERP study.

    abstract::Schizotypy is associated with poor emotion regulation that is thought to contribute to the development of psychotic symptoms and to indicate a predisposition to schizophrenia. Having focused primarily on the relationship between schizotypy and explicit emotion regulation, existing studies have, until now, neglected to...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60787-9

    authors: Hoid D,Pan DN,Wang Y,Li X

    更新日期:2020-03-03 00:00:00

  • Can enzyme proximity accelerate cascade reactions?

    abstract::The last decade has seen an exponential expansion of interest in conjugating multiple enzymes of cascades in close proximity to each other, with the overarching goal being to accelerate the overall reaction rate. However, some evidence has emerged that there is no effect of proximity channeling on the reaction velocit...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-37034-3

    authors: Kuzmak A,Carmali S,von Lieres E,Russell AJ,Kondrat S

    更新日期:2019-01-24 00:00:00

  • Comprehensive map of age-associated splicing changes across human tissues and their contributions to age-associated diseases.

    abstract::Alternative splicing contributes to phenotypic diversity at multiple biological scales, and its dysregulation is implicated in both ageing and age-associated diseases in human. Cross-tissue variability in splicing further complicates its links to age-associated phenotypes and elucidating these links requires a compreh...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29086-2

    authors: Wang K,Wu D,Zhang H,Das A,Basu M,Malin J,Cao K,Hannenhalli S

    更新日期:2018-07-19 00:00:00

  • Micro-epidemiology and spatial heterogeneity of P. vivax parasitaemia in riverine communities of the Peruvian Amazon: A multilevel analysis.

    abstract::Malaria has steadily increased in the Peruvian Amazon over the last five years. This study aimed to determine the parasite prevalence and micro-geographical heterogeneity of Plasmodium vivax parasitaemia in communities of the Peruvian Amazon. Four cross-sectional active case detection surveys were conducted between Ma...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07818-0

    authors: Carrasco-Escobar G,Gamboa D,Castro MC,Bangdiwala SI,Rodriguez H,Contreras-Mancilla J,Alava F,Speybroeck N,Lescano AG,Vinetz JM,Rosas-Aguirre A,Llanos-Cuentas A

    更新日期:2017-08-14 00:00:00

  • Low brain endocannabinoids associated with persistent non-goal directed nighttime hyperactivity after traumatic brain injury in mice.

    abstract::Traumatic brain injury (TBI) is a frequent cause of chronic headache, fatigue, insomnia, hyperactivity, memory deficits, irritability and posttraumatic stress disorder. Recent evidence suggests beneficial effects of pro-cannabinoid treatments. We assessed in mice levels of endocannabinoids in association with the occu...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-71879-x

    authors: Vogel A,Wilken-Schmitz A,Hummel R,Lang M,Gurke R,Schreiber Y,Schäfer MKE,Tegeder I

    更新日期:2020-09-10 00:00:00

  • In vitro dexamethasone treatment does not induce alternative ATM transcripts in cells from Ataxia-Telangiectasia patients.

    abstract::Short term treatment with low doses of glucocorticoid analogues has been shown to ameliorate neurological symptoms in Ataxia-Telangiectasia (A-T), a rare autosomal recessive multisystem disease that mainly affects the cerebellum, immune system, and lungs. Molecular mechanisms underlying this clinical observation are u...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77352-z

    authors: Pozzi E,Giorgio E,Mancini C,Lo Buono N,Augeri S,Ferrero M,Di Gregorio E,Riberi E,Vinciguerra M,Nanetti L,Bianchi FT,Sassi MP,Costanzo V,Mariotti C,Funaro A,Cavalieri S,Brusco A

    更新日期:2020-11-19 00:00:00

  • Thermodynamic Stability and Structural Insights for CH3NH3Pb1-xSixI3, CH3NH3Pb1-xGexI3, and CH3NH3Pb1-xSnxI3 Hybrid Perovskite Alloys: A Statistical Approach from First Principles Calculations.

    abstract::The recent reaching of 20% of conversion efficiency by solar cells based on metal hybrid perovskites (MHP), e.g., the methylammonium (MA) lead iodide, CH3NH3PbI3 (MAPbI3), has excited the scientific community devoted to the photovoltaic materials. However, the toxicity of Pb is a hindrance for large scale commercial o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47192-7

    authors: Guedes-Sobrinho D,Guilhon I,Marques M,Teles LK

    更新日期:2019-07-30 00:00:00

  • Prognostic signature of lung adenocarcinoma based on stem cell-related genes.

    abstract::Lung adenocarcinoma (LUAD) is characterized by high infiltration and rapid growth. The function of the stem cell population is to control and maintain cell regeneration. Therefore, it is necessary to study the prognostic value of stem cell-related genes in LUAD. Signature genes were screened out from 166 stem cell-rel...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-80453-4

    authors: Huang Z,Shi M,Zhou H,Wang J,Zhang HJ,Shi J-

    更新日期:2021-01-18 00:00:00

  • Accelerated age-related olfactory decline among type 1 Usher patients.

    abstract::Usher Syndrome (USH) is a rare disease with hearing loss, retinitis pigmentosa and, sometimes, vestibular dysfunction. A phenotype heterogeneity is reported. Recent evidence indicates that USH is likely to belong to an emerging class of sensory ciliopathies. Olfaction has recently been implicated in ciliopathies, but ...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章

    doi:10.1038/srep28309

    authors: Ribeiro JC,Oliveiros B,Pereira P,António N,Hummel T,Paiva A,Silva ED

    更新日期:2016-06-22 00:00:00

  • 3D-QSAR and docking studies of flavonoids as potent Escherichia coli inhibitors.

    abstract::Flavonoids are potential antibacterial agents. However, key substituents and mechanism for their antibacterial activity have not been fully investigated. The quantitative structure-activity relationship (QSAR) and molecular docking of flavonoids relating to potent anti-Escherichia coli agents were investigated. Compar...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep23634

    authors: Fang Y,Lu Y,Zang X,Wu T,Qi X,Pan S,Xu X

    更新日期:2016-04-06 00:00:00

  • Dibutyl phthalate alters the metabolic pathways of microbes in black soils.

    abstract::Dibutyl phthalate (DBP) is well known as a high-priority pollutant. This study explored the impacts of DBP on the metabolic pathways of microbes in black soils in the short term (20 days). The results showed that the microbial communities were changed in black soils with DBP. In nitrogen cycling, the abundances of the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21030-8

    authors: Xu W,You Y,Wang Z,Chen W,Zeng J,Zhao X,Su Y

    更新日期:2018-02-08 00:00:00

  • Exploring the effect of nsSNPs in human YPEL3 gene in cellular senescence.

    abstract::YPEL3 that induces cellular senescence in both normal and tumour cells of humans may show altered expression under the influence of incidental mutations. In this study, we proposed the first structure of Native YPEL3 protein and its five possible deleterious mutants-V40M, C61Y, G98R, G108S, and A131T and predicted the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72333-8

    authors: Singh A,Thakur M,Singh SK,Sharma LK,Chandra K

    更新日期:2020-09-17 00:00:00

  • Fourier ptychographic reconstruction using Poisson maximum likelihood and truncated Wirtinger gradient.

    abstract::Fourier ptychographic microscopy (FPM) is a novel computational coherent imaging technique for high space-bandwidth product imaging. Mathematically, Fourier ptychographic (FP) reconstruction can be implemented as a phase retrieval optimization process, in which we only obtain low resolution intensity images correspond...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27384

    authors: Bian L,Suo J,Chung J,Ou X,Yang C,Chen F,Dai Q

    更新日期:2016-06-10 00:00:00

  • Anthropogenic effects on a tropical forest according to the distance from human settlements.

    abstract::The protection of tropical forests is one of the most urgent issues in conservation biology because of the rapid deforestation that has occurred over the last 50 years. Even in protected forests, the anthropogenic effects from newly expanding villages such as harvesting of medicinal plants, pasturing cattle and forest...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep14689

    authors: Popradit A,Srisatit T,Kiratiprayoon S,Yoshimura J,Ishida A,Shiyomi M,Murayama T,Chantaranothai P,Outtaranakorn S,Phromma I

    更新日期:2015-10-05 00:00:00

  • Complex rice systems to improve rice yield and yield stability in the face of variable weather conditions.

    abstract::Extreme weather events and pest outbreaks decrease rice yields and increase their variability, presenting challenges for the agricultural agenda to increase rice productivity and yield stability in Asia. The integration of azolla, fish and ducks has been shown to create robust systems that maintain high yields under h...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-32915-z

    authors: Khumairoh U,Lantinga EA,Schulte RPO,Suprayogo D,Groot JCJ

    更新日期:2018-10-03 00:00:00

  • Membrane glycerolipidome of soybean root hairs and its response to nitrogen and phosphate availability.

    abstract::Root hairs are tubular extensions of specific root epidermal cells important in plant nutrition and water absorption. To determine membrane glycerolipids in root hairs and roots may differ, as well as their respective response to nutrient availability, this study analyzed the membrane glycerolipid species in soybean r...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36172

    authors: Wei F,Fanella B,Guo L,Wang X

    更新日期:2016-11-04 00:00:00

  • The induced by an electromagnetic field coexistence of types I and II spectra in Weyl semimetals.

    abstract::Due to their unique properties, Weyl semimetals (WSMs) are promising materials for the future electronics. Currently, the two types (I and II) of WSMs are discovered experimentally. These types of WSMs differ from each other in their topological properties. In this paper we showed that a coexistence of types I and II ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-32104-y

    authors: Alisultanov ZZ

    更新日期:2018-09-12 00:00:00

  • Interactive phenotyping of large-scale histology imaging data with HistomicsML.

    abstract::Whole-slide imaging of histologic sections captures tissue microenvironments and cytologic details in expansive high-resolution images. These images can be mined to extract quantitative features that describe tissues, yielding measurements for hundreds of millions of histologic objects. A central challenge in utilizin...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15092-3

    authors: Nalisnik M,Amgad M,Lee S,Halani SH,Velazquez Vega JE,Brat DJ,Gutman DA,Cooper LAD

    更新日期:2017-11-06 00:00:00

  • Pan-genome analysis and ancestral state reconstruction of class halobacteria: probability of a new super-order.

    abstract::Halobacteria, a class of Euryarchaeota are extremely halophilic archaea that can adapt to a wide range of salt concentration generally from 10% NaCl to saturated salt concentration of 32% NaCl. It consists of the orders: Halobacteriales, Haloferaciales and Natriabales. Pan-genome analysis of class Halobacteria was don...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77723-6

    authors: Gaba S,Kumari A,Medema M,Kaushik R

    更新日期:2020-12-03 00:00:00

  • Loss of Stemness, EMT, and Supernumerary Tooth Formation in Cebpb-/-Runx2+/- Murine Incisors.

    abstract::Adult Cebpb KO mice incisors present amelogenin-positive epithelium pearls, enamel and dentin allopathic hyperplasia, fewer Sox2-positive cells in labial cervical loop epitheliums, and reduced Sox2 expression in enamel epithelial stem cells. Thus, Cebpb acts upstream of Sox2 to regulate stemness. In this study, Cebpb ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-23515-y

    authors: Saito K,Takahashi K,Huang B,Asahara M,Kiso H,Togo Y,Tsukamoto H,Mishima S,Nagata M,Iida M,Tokita Y,Asai M,Shimizu A,Komori T,Harada H,MacDougall M,Sugai M,Bessho K

    更新日期:2018-03-26 00:00:00