Assessing mutant p53 in primary high-grade serous ovarian cancer using immunohistochemistry and massively parallel sequencing.

Abstract:

:The tumour suppressor p53 is mutated in cancer, including over 96% of high-grade serous ovarian cancer (HGSOC). Mutations cause loss of wild-type p53 function due to either gain of abnormal function of mutant p53 (mutp53), or absent to low mutp53. Massively parallel sequencing (MPS) enables increased accuracy of detection of somatic variants in heterogeneous tumours. We used MPS and immunohistochemistry (IHC) to characterise HGSOCs for TP53 mutation and p53 expression. TP53 mutation was identified in 94% (68/72) of HGSOCs, 62% of which were missense. Missense mutations demonstrated high p53 by IHC, as did 35% (9/26) of non-missense mutations. Low p53 was seen by IHC in 62% of HGSOC associated with non-missense mutations. Most wild-type TP53 tumours (75%, 6/8) displayed intermediate p53 levels. The overall sensitivity of detecting a TP53 mutation based on classification as 'Low', 'Intermediate' or 'High' for p53 IHC was 99%, with a specificity of 75%. We suggest p53 IHC can be used as a surrogate marker of TP53 mutation in HGSOC; however, this will result in misclassification of a proportion of TP53 wild-type and mutant tumours. Therapeutic targeting of mutp53 will require knowledge of both TP53 mutations and mutp53 expression.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Cole AJ,Dwight T,Gill AJ,Dickson KA,Zhu Y,Clarkson A,Gard GB,Maidens J,Valmadre S,Clifton-Bligh R,Marsh DJ

doi

10.1038/srep26191

subject

Has Abstract

pub_date

2016-05-18 00:00:00

pages

26191

issn

2045-2322

pii

srep26191

journal_volume

6

pub_type

杂志文章
  • Geometry of quantum computation with qudits.

    abstract::The circuit complexity of quantum qubit system evolution as a primitive problem in quantum computation has been discussed widely. We investigate this problem in terms of qudit system. Using the Riemannian geometry the optimal quantum circuits are equivalent to the geodetic evolutions in specially curved parametrizatio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep04044

    authors: Luo MX,Chen XB,Yang YX,Wang X

    更新日期:2014-02-10 00:00:00

  • K-core robustness in ecological and financial networks.

    abstract::In many real-world networks, the ability to withstand targeted or global attacks; extinctions; or shocks is vital to the survival of the network itself, and of dependent structures such as economies (for financial networks) or even the planet (for ecosystems). Previous attempts to characterise robustness include neste...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-59959-4

    authors: Burleson-Lesser K,Morone F,Tomassone MS,Makse HA

    更新日期:2020-02-25 00:00:00

  • Viral Evolution and Cytotoxic T Cell Restricted Selection in Acute Infant HIV-1 Infection.

    abstract::Antiretroviral therapy-naive HIV-1 infected infants experience poor viral containment and rapid disease progression compared to adults. Viral factors (e.g. transmitted cytotoxic T- lymphocyte (CTL) escape mutations) or infant factors (e.g. reduced CTL functional capacity) may explain this observation. We assessed CTL ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29536

    authors: Garcia-Knight MA,Slyker J,Payne BL,Pond SL,de Silva TI,Chohan B,Khasimwa B,Mbori-Ngacha D,John-Stewart G,Rowland-Jones SL,Esbjörnsson J

    更新日期:2016-07-12 00:00:00

  • Single-crystalline, wormlike hematite photoanodes for efficient solar water splitting.

    abstract::A hematite photoanode showing a stable, record-breaking performance of 4.32 mA/cm² photoelectrochemical water oxidation current at 1.23 V vs. RHE under simulated 1-sun (100 mW/cm²) irradiation is reported. This photocurrent corresponds to ca. 34% of the maximum theoretical limit expected for hematite with a band gap o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep02681

    authors: Kim JY,Magesh G,Youn DH,Jang JW,Kubota J,Domen K,Lee JS

    更新日期:2013-01-01 00:00:00

  • Video-rate volumetric neuronal imaging using 3D targeted illumination.

    abstract::Fast volumetric microscopy is required to monitor large-scale neural ensembles with high spatio-temporal resolution. Widefield fluorescence microscopy can image large 2D fields of view at high resolution and speed while remaining simple and costeffective. A focal sweep add-on can further extend the capacity of widefie...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26240-8

    authors: Xiao S,Tseng HA,Gritton H,Han X,Mertz J

    更新日期:2018-05-21 00:00:00

  • Dike intrusions during rifting episodes obey scaling relationships similar to earthquakes.

    abstract::As continental rifts evolve towards mid-ocean ridges, strain is accommodated by repeated episodes of faulting and magmatism. Discrete rifting episodes have been observed along two subaerial divergent plate boundaries, the Krafla segment of the Northern Volcanic Rift Zone in Iceland and the Manda-Hararo segment of the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03886

    authors: Passarelli L,Rivalta E,Shuler A

    更新日期:2014-01-28 00:00:00

  • Group A Streptococcus establishes pharynx infection by degrading the deoxyribonucleic acid of neutrophil extracellular traps.

    abstract::Group A Streptococcus (GAS) secretes deoxyribonucleases and evades neutrophil extracellular killing by degrading neutrophil extracellular traps (NETs). However, limited information is currently available on the interaction between GAS and NETs in the pathogenicity of GAS pharyngitis. In this study, we modified a mouse...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60306-w

    authors: Tanaka M,Kinoshita-Daitoku R,Kiga K,Sanada T,Zhu B,Okano T,Aikawa C,Iida T,Ogura Y,Hayashi T,Okubo K,Kurosawa M,Hirahashi J,Suzuki T,Nakagawa I,Nangaku M,Mimuro H

    更新日期:2020-02-24 00:00:00

  • Author Correction: Androgen receptor is a potential novel prognostic marker and oncogenic target in osteosarcoma with dependence on CDK11.

    abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-018-30644-x

    authors: Liao Y,Sassi S,Halvorsen S,Feng Y,Shen J,Gao Y,Cote G,Choy E,Harmon D,Mankin H,Hornicek F,Duan Z

    更新日期:2018-08-09 00:00:00

  • Down-regulation of vascular GLP-1 receptor expression in human subjects with obesity.

    abstract::It has been thought that incretin signaling prevents arteriosclerosis, and very recently anti-arteriosclerotic effects through GLP-1 receptor were finally demonstrated in clinical human study. The purpose of this study was to investigate how vascular GLP-1 receptor expression is influenced in human subjects. First, we...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-28849-1

    authors: Kimura T,Obata A,Shimoda M,Shimizu I,da Silva Xavier G,Okauchi S,Hirukawa H,Kohara K,Mune T,Moriuchi S,Hiraoka A,Tamura K,Chikazawa G,Ishida A,Yoshitaka H,Rutter GA,Kaku K,Kaneto H

    更新日期:2018-07-13 00:00:00

  • Selective suppression of rapid eye movement sleep increases next-day negative affect and amygdala responses to social exclusion.

    abstract::Healthy sleep, positive general affect, and the ability to regulate emotional experiences are fundamental for well-being. In contrast, various mental disorders are associated with altered rapid eye movement (REM) sleep, negative affect, and diminished emotion regulation abilities. However, the neural processes mediati...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74169-8

    authors: Glosemeyer RW,Diekelmann S,Cassel W,Kesper K,Koehler U,Westermann S,Steffen A,Borgwardt S,Wilhelm I,Müller-Pinzler L,Paulus FM,Krach S,Stolz DS

    更新日期:2020-10-14 00:00:00

  • Overcoming evolved resistance to population-suppressing homing-based gene drives.

    abstract::The recent development of a CRISPR-Cas9-based homing system for the suppression of Anopheles gambiae is encouraging; however, with current designs, the slow emergence of homing-resistant alleles is expected to result in suppressed populations rapidly rebounding, as homing-resistant alleles have a significant fitness a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-02744-7

    authors: Marshall JM,Buchman A,Sánchez C HM,Akbari OS

    更新日期:2017-06-19 00:00:00

  • Incoherent Nuclear Resonant Scattering from a Standing Spin Wave.

    abstract::We introduce a method to study the spatial profiles of standing spin waves in ferromagnetic microstructures. The method relies on Nuclear Resonant Scattering of 57Fe using a microfocused beam of synchrotron radiation, the transverse coherence length of which is smaller than the length scale of lateral variations in th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29596-z

    authors: Gollwitzer J,Bocklage L,Schlage K,Herlitschke M,Wille HC,Leupold O,Adolff CF,Meier G,Röhlsberger R

    更新日期:2018-07-26 00:00:00

  • A meta-analysis of the efficacy of HAART on HIV transmission and its impact on sexual risk behaviours among men who have sex with men.

    abstract::Evidence showed preventive impacts of the highly active antiretroviral therapy (HAART) on the Human Immunodeficiency Virus (HIV) transmission amomg heterosexual population, however, that is of deficit among men who have sex with men (MSM). The aim was to systematically examine the efficacy of HAART on HIV transmission...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-019-56530-8

    authors: Jiang Y,Su S,Borné Y

    更新日期:2020-01-23 00:00:00

  • Clinical Utility of Intraoperative Tympanomastoidectomy Assessment Using a Surgical Microscope Integrated with an Optical Coherence Tomography.

    abstract::Significant technical and optical advances are required for intraoperative optical coherence tomography (OCT) to be utilized during otological surgeries. Integrating OCT with surgical microscopy makes it possible to evaluate soft tissue in real-time and at a high resolution. Herein, we describe an augmented-reality, i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-35563-5

    authors: Lee J,Wijesinghe RE,Jeon D,Kim P,Choung YH,Jang JH,Jeon M,Kim J

    更新日期:2018-11-27 00:00:00

  • Intracellular immunization against HIV infection with an intracellular antibody that mimics HIV integrase binding to the cellular LEDGF protein.

    abstract::Preventing the protein-protein interaction of the cellular chromatin binding protein Lens Epithelium-Derived Growth Factor (LEDGF) and human immunodeficiency virus (HIV) integrase is an important possible strategy for anti-viral treatment for AIDS. We have used Intracellular Antibody Capture technology to isolate a si...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16742-2

    authors: Bao L,Hannon C,Cruz-Mignoni A,Ptchelkine D,Sun MY,Miller A,Bunjobpol W,Quevedo CE,Derveni M,Chambers J,Simmons A,Phillips SEV,Rabbitts TH

    更新日期:2017-12-04 00:00:00

  • Specific strains of Streptococcus mutans, a pathogen of dental caries, in the tonsils, are associated with IgA nephropathy.

    abstract::Streptococcus mutans is known to be a major causative agent of dental caries, and strains expressing the cell surface collagen-binding Cnm protein contribute to the development of several systemic diseases. A relationship between tonsillar immunity and glomerulonephritis has been recognized in IgA nephropathy (IgAN), ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56679-2

    authors: Ito S,Misaki T,Naka S,Wato K,Nagasawa Y,Nomura R,Otsugu M,Matsumoto-Nakano M,Nakano K,Kumagai H,Oshima N

    更新日期:2019-12-27 00:00:00

  • Recurrent SARS-CoV-2 RNA positivity after COVID-19: a systematic review and meta-analysis.

    abstract::Present study aimed to estimate the incidence of recurrent SARS-CoV-2 RNA positivity after recovery from COVID-19 and to determine the factors associated with recurrent positivity. We searched the PubMed, MedRxiv, BioRxiv, the Cochrane Library, ClinicalTrials.gov, and the World Health Organization International Clinic...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-020-77739-y

    authors: Azam M,Sulistiana R,Ratnawati M,Fibriana AI,Bahrudin U,Widyaningrum D,Aljunid SM

    更新日期:2020-11-26 00:00:00

  • Weight change after smoking cessation and incident metabolic syndrome in middle-aged Korean men: an observational study.

    abstract::We aimed to examine the effect of weight change attributable to cessation of cigarette smoking on newly diagnosed metabolic syndrome (MetS). We prospectively followed 5,809 men aged between 40 to 69 years without MetS at baseline in the Health Examinees-Gem (HEXA-G) study up to 4 years. The participants were grouped i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39811-0

    authors: Kim K,Choi S,Lee JK,Choi JY,Shin A,Park SK,Kang D,Park SM

    更新日期:2019-02-28 00:00:00

  • Direct determination of three-phase contact line properties on nearly molecular scale.

    abstract::Wetting phenomena in multi-phase systems govern the shape of the contact line which separates the different phases. For liquids in contact with solid surfaces wetting is typically described in terms of contact angle. While in macroscopic systems the contact angle can be determined experimentally, on the molecular scal...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26111

    authors: Winkler PM,McGraw RL,Bauer PS,Rentenberger C,Wagner PE

    更新日期:2016-05-17 00:00:00

  • Influence of Roasting Condition on Flavor Profile of Sunflower Seeds: A flavoromics approach.

    abstract::Sunflower see/ds (Helianthus annuus L.) were roasted in an electric forced air oven for 15, 30, 45, and 60 min at 125, 135 and 145 °C. The effect of temperature and time on the flavor profile of the samples were evaluated by headspace solid-phase microextraction coupled with gas chromatography-mass spectroscopy (HS-SP...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-47811-3

    authors: Guo S,Na Jom K,Ge Y

    更新日期:2019-08-05 00:00:00

  • Lysophosphatidic acids and their substrate lysophospholipids in cerebrospinal fluid as objective biomarkers for evaluating the severity of lumbar spinal stenosis.

    abstract::Lysophospholipids (LPLs) are known to have potentially important roles in the initiation and maintenance of neuropathic pain in animal models. This study investigated the association between the clinical severity of lumbar spinal stenosis (LSS) and the cerebrospinal fluid (CSF) levels of LPLs, using human samples. We ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-45742-7

    authors: Hayakawa K,Kurano M,Ohya J,Oichi T,Kano K,Nishikawa M,Uranbileg B,Kuwajima K,Sumitani M,Tanaka S,Aoki J,Yatomi Y,Chikuda H

    更新日期:2019-06-24 00:00:00

  • Data-based Decision Rules to Personalize Depression Follow-up.

    abstract::Depression is a common mental illness with complex and heterogeneous progression dynamics. Risk grouping of depression treatment population based on their longitudinal patterns has the potential to enable cost-effective monitoring policy design. This paper establishes a rule-based method to identify a set of risk pred...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-23326-1

    authors: Lin Y,Huang S,Simon GE,Liu S

    更新日期:2018-03-22 00:00:00

  • Dynamical complexity and computation in recurrent neural networks beyond their fixed point.

    abstract::Spontaneous activity found in neural networks usually results in a reduction of computational performance. As a consequence, artificial neural networks are often operated at the edge of chaos, where the network is stable yet highly susceptible to input information. Surprisingly, regular spontaneous dynamics in Neural ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21624-2

    authors: Marquez BA,Larger L,Jacquot M,Chembo YK,Brunner D

    更新日期:2018-02-20 00:00:00

  • Evidence of Cardiovascular Calcification and Fibrosis in Pseudoxanthoma Elasticum Mouse Models Subjected to DOCA-Salt Hypertension.

    abstract::Pseudoxanthoma Elasticum (PXE) is a rare disorder characterized by fragmentation and progressive calcification of elastic fibres in connective tissues. Although arterial hypertension (AHT) has been reported in PXE patients, its impact on pathological manifestations has as yet been unexplored. We investigated the conse...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-52808-z

    authors: Omarjee L,Roy C,Leboeuf C,Favre J,Henrion D,Mahe G,Leftheriotis G,Martin L,Janin A,Kauffenstein G

    更新日期:2019-11-08 00:00:00

  • High-fat diet-induced obesity affects alpha 7 nicotine acetylcholine receptor expressions in mouse lung myeloid cells.

    abstract::Ample evidence indicates that obesity causes dysfunctions in the lung. Previous studies also show that cholinergic anti-inflammatory pathways play crucial roles in obesity-induced chronic inflammation via α7 nicotinic acetylcholine receptor (α7nAChR) signaling. However, it remains unclear whether and how obesity affec...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-75414-w

    authors: Qi Y,Si D,Zhu L,Qi Y,Wu Z,Chen D,Yang Y

    更新日期:2020-10-27 00:00:00

  • High information capacity DNA-based data storage with augmented encoding characters using degenerate bases.

    abstract::DNA-based data storage has emerged as a promising method to satisfy the exponentially increasing demand for information storage. However, practical implementation of DNA-based data storage remains a challenge because of the high cost of data writing through DNA synthesis. Here, we propose the use of degenerate bases a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-43105-w

    authors: Choi Y,Ryu T,Lee AC,Choi H,Lee H,Park J,Song SH,Kim S,Kim H,Park W,Kwon S

    更新日期:2019-04-29 00:00:00

  • Fabrication of self-assembled spherical Gold Particles by pulsed UV Laser Treatment.

    abstract::We report on the fabrication of spherical Au spheres by pulsed laser treatment using a KrF excimer laser (248 nm, 25 ns) under ambient conditions as a fast and high throughput fabrication technique. The presented experiments were realized using initial Au layers of 100 nm thickness deposited on optically transparent a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-29661-7

    authors: Schmidl G,Jia G,Gawlik A,Kreusch J,Schmidl F,Dellith J,Dathe A,Lin ZH,Huang JS,Plentz J

    更新日期:2018-07-26 00:00:00

  • High-performance transparent and stretchable all-solid supercapacitors based on highly aligned carbon nanotube sheets.

    abstract::By using highly aligned carbon nanotube (CNT) sheets of excellent optical transmittance and mechanical stretchability as both the current collector and active electrode, high-performance transparent and stretchable all-solid supercapacitors with a good stability were developed. A transmittance up to 75% at the wavelen...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03612

    authors: Chen T,Peng H,Durstock M,Dai L

    更新日期:2014-01-09 00:00:00

  • In situ structural characterization of early amyloid aggregates in Alzheimer's disease transgenic mice and Octodon degus.

    abstract::Amyloid plaques composed of Aβ amyloid peptides and neurofibrillary tangles are a pathological hallmark of Alzheimer's disease. In situ identification of early-stage amyloid aggregates in Alzheimer's disease is relevant for their importance as potential targets for effective drugs. Synchrotron-based infrared imaging i...

    journal_title:Scientific reports

    pub_type: 杂志文章,收录出版

    doi:10.1038/s41598-020-62708-2

    authors: Benseny-Cases N,Álvarez-Marimon E,Aso E,Carmona M,Klementieva O,Appelhans D,Ferrer I,Cladera J

    更新日期:2020-04-03 00:00:00

  • Activation of cardiac AMPK-FGF21 feed-forward loop in acute myocardial infarction: Role of adrenergic overdrive and lipolysis byproducts.

    abstract::Fibroblast growth factor 21 (FGF21) is a metabolic hormone having anti-oxidative and anti-hypertrophic effects. However, the regulation of FGF21 expression during acute myocardial infarction (AMI) remains unclear. We tested blood samples from 50 patients with AMI and 43 patients with stable angina pectoris (sAP) for F...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-48356-1

    authors: Sunaga H,Koitabashi N,Iso T,Matsui H,Obokata M,Kawakami R,Murakami M,Yokoyama T,Kurabayashi M

    更新日期:2019-08-14 00:00:00