Abstract：
:The global DNA methylation degree may be a ubiquitous and early biomarker to distinguish cancer cells from benign cells. However, its usefulness in clinical diagnosis was scarcely demonstrated, because the cancer cells isolated from patients were usually very rare. Even if 10 mL of peripheral blood was sampled from a patient, only tens of cancer cells could be isolated. So a method to quantify DNA methylation from small number of cells was needed to apply DNA methylation in clinical environment. In this study, we found that normal breast cell line MCF10A and breast cancer cell line MCF7 cells present significantly different percentage of genomic 5-methylcytosine (p < 0.02, n = 8), it could be a potential indicator for rapid discrimination of rare cancer cells from normal cells. However, conventional mass spectrometry needs usually ~10(6) cells to quantify DNA methylation degree, which was too large to be applied in clinical diagnosis. Here we developed a fast mass spectrometry-based method capable of analyzing the DNA methylation degree from only ~100 human cells. Our method could reveal the different DNA methylation degree between MCF10A and MCF7 cells in less than two hours, having the potential to provide reliable information for clinical application.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Si X,Zhao Y,Yang C,Zhang S,Zhang Xdoi
10.1038/srep11882subject
Has Abstractpub_date
2015-07-03 00:00:00pages
11882issn
2045-2322pii
srep11882journal_volume
5pub_type
杂志文章abstract：:All-trans-retinoic acid (atRA), the active metabolite of vitamin A, is a critical signaling molecule during embryonic and fetal development and is necessary for maternal health. Fetal exposure to endogenous atRA is tightly regulated during gestation in a tissue specific manner and maternal exposure to exogenous retino...
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pub_type： 临床试验,杂志文章
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pub_type： 杂志文章,已发布勘误
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2014-06-26 00:00:00
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