Abstract：
:In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current models and humans. Here, we fabricated a cancer microtissue array in a multi-well format that exhibits heterogeneous and batch-to-batch structure by continuous deposition of collagen-suspended Hela cells on a fibroblast-layered nanofibrous membrane via inkjet printing. Expression of both Matrix Metalloproteinase 2 (MMP2) and Matrix Metalloproteinase 9 (MMP9) was higher in cancer microtissues than in fibroblast-free microtissues. The fabricated microtissues were treated with an anticancer drug, and high drug resistance to doxorubicin occurred in cancer microtissues but not in fibroblast-free microtissues. These results introduce an inkjet printing fabrication method for cancer microtissue arrays, which can be used for various applications such as early drug screening and gradual 3D cancer studies.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Park TM,Kang D,Jang I,Yun WS,Shim JH,Jeong YH,Kwak JY,Yoon S,Jin Sdoi
10.3390/ijms18112348subject
Has Abstractpub_date
2017-11-07 00:00:00issue
11issn
1422-0067pii
ijms18112348journal_volume
18pub_type
杂志文章abstract：:The application of nanotechnology for the treatment of cancer is mostly based on early tumor detection and diagnosis by nanodevices capable of selective targeting and delivery of chemotherapeutic drugs to the specific tumor site. Due to the remarkable properties of gold nanoparticles, they have long been considered as...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2018-07-06 00:00:00
abstract：:The deubiquitination of histone H2A on lysine 119 by 2A-DUB/MYSM1, BAP1, USP16, and other enzymes is required for key cellular processes, including transcriptional activation, apoptosis, and cell cycle control, during normal hematopoiesis and tissue development, and in tumor cells. Based on our finding that MYSM1 colo...
journal_title：International journal of molecular sciences
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abstract：:Neuroblastoma is a common childhood cancer with almost a third of those affected still dying, thus new therapeutic strategies need to be explored. Current experimental therapies focus mostly on inhibiting oncogenic transcription factor signalling. Although LIN28B, DICER and other RNA-binding proteins (RBPs) have repor...
journal_title：International journal of molecular sciences
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更新日期：2020-07-19 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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abstract：:The cytostatic agent hydroxyurea (HU) has proven to be beneficial for a variety of conditions in the disciplines of oncology, hematology, infectious disease and dermatology. It disrupts the S phase of the cell cycle by inhibiting the ribonucleotide reductase enzyme, thus blocking the transformation of ribonucleotides ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2018-12-11 00:00:00
abstract：:This study evaluated frequency-dependent effects of extremely low frequency electromagnetic fields (ELF-EMFs) on melanogenesis by melanocytes in vitro. Melanocytes were exposed to 2 mT EMFs at 30-75 Hz for 3 days before melanogenesis was examined. Exposure to ELF-EMFs at 50 and 60 Hz induced melanogenic maturation wit...
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2017-12-23 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2017-04-06 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18081680
更新日期：2017-08-02 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12117314
更新日期：2011-01-01 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140918078
更新日期：2013-09-04 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124218
更新日期：2020-06-13 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21051720
更新日期：2020-03-03 00:00:00
abstract：:Dry eye syndrome is the most common eye disease and it is caused by various reasons. As the balance of the tear film that protects the eyes is broken due to various causes, it becomes impossible to properly protect the eyes. In this study, the protective effects and underlying mechanisms of topical (E)-4-(2-(6-(2,6-di...
journal_title：International journal of molecular sciences
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更新日期：2020-05-25 00:00:00
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journal_title：International journal of molecular sciences
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doi：10.3390/ijms18102086
更新日期：2017-10-20 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2017-06-02 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2017-08-01 00:00:00
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journal_title：International journal of molecular sciences
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doi：10.3390/ijms17091398
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journal_title：International journal of molecular sciences
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更新日期：2015-09-15 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2014-11-21 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2015-10-14 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-05-16 00:00:00
abstract：:Matrix Gla Protein (MGP), a small Gla vitamin K-dependent protein, is the most powerful natural occurring inhibitor of calcification in the human body. To become biologically active, MGP must undergo vitamin K-dependent carboxylation and phosphorylation. Vitamin K deficiency leads to the inactive uncarboxylated, depho...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2019-02-01 00:00:00
abstract：:Ascorbate oxidase (AO) plays important roles in plant growth and development. Previously, we reported a cotton AO gene that acts as a positive factor in cell growth. Investigations on Gossypium hirsutum AO (GhAO) family genes and their multiple functions are limited. The present study identified eight GhAO family gene...
journal_title：International journal of molecular sciences
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doi：10.3390/ijms20246167
更新日期：2019-12-06 00:00:00
abstract：:Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the struct...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19102912
更新日期：2018-09-25 00:00:00
abstract：:Spirulina maxima, a microalga containing high levels of protein and many polyphenols, including chlorophyll a and C-phycocyanin, has antioxidant and anti-inflammatory therapeutic effects. However, the mechanisms where by Spirulina maxima ameliorates cognitive disorders induced by amyloid-β 1-42 (Aβ1-42) are not fully ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18112401
更新日期：2017-11-13 00:00:00
