Abstract：
:The basic structural and functional unit of a living organism is a single cell. To understand the variability and to improve the biomedical requirement of a single cell, its analysis has become a key technique in biological and biomedical research. With a physical boundary of microchannels and microstructures, single cells are efficiently captured and analyzed, whereas electric forces sort and position single cells. Various microfluidic techniques have been exploited to manipulate single cells through hydrodynamic and electric forces. Digital microfluidics (DMF), the manipulation of individual droplets holding minute reagents and cells of interest by electric forces, has received more attention recently. Because of ease of fabrication, compactness and prospective automation, DMF has become a powerful approach for biological application. We review recent developments of various microfluidic chips for analysis of a single cell and for efficient genetic screening. In addition, perspectives to develop analysis of single cells based on DMF and emerging functionality with high throughput are discussed.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
He JL,Chen AT,Lee JH,Fan SKdoi
10.3390/ijms160922319subject
Has Abstractpub_date
2015-09-15 00:00:00pages
22319-32issue
9issn
1422-0067pii
ijms160922319journal_volume
16pub_type
杂志文章,评审abstract：:Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21134774
更新日期：2020-07-05 00:00:00
abstract：:High-density lipoproteins (HDL) are known to have vasoprotective functions in peripheral arteries and many of these functions extend to brain-derived endothelial cells. Importantly, several novel brain-relevant HDL functions have been discovered using brain endothelial cells and in 3D bioengineered human arteries. The...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20030462
更新日期：2019-01-22 00:00:00
abstract：:Metabolic reprogramming that favors high glycolytic flux with lactate production in normoxia is among cancer hallmarks. Lactate is an essential oncometabolite regulating cellular redox homeostasis, energy substrate partitioning, and intracellular signaling. Moreover, malignant phenotype's chief characteristics are dep...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21249676
更新日期：2020-12-18 00:00:00
abstract：:Riboswitches, which are located within certain noncoding RNA region perform functions as genetic "switches", regulating when and where genes are expressed in response to certain ligands. Understanding the numerous functions of riboswitches requires computation models to predict structures and structural changes of the...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms18112442
更新日期：2017-11-17 00:00:00
abstract：:Alterations to the programmed cell death protein-1 (PD-1) pathway were previously shown to be involved in a poorer prognosis for patients with proliferative glomerulonephritis (PGN). Here, we investigated the association between several infectious agents and the expression of PD-1 and its ligand (PD-L1) on T and B lym...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218001
更新日期：2020-10-27 00:00:00
abstract：:Increasing photosynthetic ability as a whole is essential for acquiring higher crop yields. Nonleaf green organs (NLGOs) make important contributions to photosynthate formation, especially under stress conditions. However, there is little information on the pod wall in legume forage related to seed development and yie...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124457
更新日期：2020-06-23 00:00:00
abstract：:Updated measurements of charged particle fluxes during the transit from Earth to Mars as well as on site measurements by Curiosity of Martian surface radiation fluxes identified potential health hazards associated with radiation exposure for human space missions. Designing mitigation strategies of radiation risks to a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20010176
更新日期：2019-01-05 00:00:00
abstract：:A series of schizonepetin derivatives have been designed and synthesized in order to obtain potent antivirus agents. The antiviral activity against HSV-1 and influenza virus H3N2 as well as the cytotoxicity of these derivatives was evaluated by using cytopathic effect (CPE) inhibition assay in vitro. Compounds M2, M4,...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms140817193
更新日期：2013-08-20 00:00:00
abstract：:3,3'-Diindolylmethane (DIM) is a bioactive compound found in Cruciferous vegetables that possesses health benefits such as antioxidant, anticancer, and anti-inflammatory effects. However, hydrophobicity and photolabile limit its pharmaceutical applications. This study aims to prepare and characterize DIM-encapsulated ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20163917
更新日期：2019-08-12 00:00:00
abstract：:Pea (Pisum sativum L.), as a major source of plant protein, is becoming one of the major cultivated crop species worldwide. In pea, the pericarp is an important determinant of the morphological characteristics and seed yield. To investigate the molecular mechanism of pericarp elongation as well as sucrose and starch a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20246135
更新日期：2019-12-05 00:00:00
abstract：:Flower development is a vital developmental process in the life cycle of woody perennials, especially fruit trees. Herein, we used transcriptomic, proteomic, and hormone analyses to investigate the key candidate genes/proteins in loquat (Eriobotrya japonica) at the stages of flower bud differentiation (FBD), floral bu...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21145107
更新日期：2020-07-20 00:00:00
abstract：:Elongation of very-long-chain fatty acids 1 (ELOVL1) is a ubiquitously expressed gene that belongs to the ELOVL family and regulates the synthesis of very-long-chain fatty acids (VLCFAs) and sphingolipids, from yeast to mammals. Mammalian target of rapamycin complex 1 (mTORC1) is a central regulator of cell metabolism...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160716440
更新日期：2015-07-20 00:00:00
abstract：:Adenosine triphosphate (ATP) has been well established as an important extracellular ligand of autocrine signaling, intercellular communication, and neurotransmission with numerous physiological and pathophysiological roles. In addition to the classical exocytosis, non-vesicular mechanisms of cellular ATP release have...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19030808
更新日期：2018-03-11 00:00:00
abstract：:The molecular pathology of diseases seen from the mitochondrial axis has become more complex with the progression of research. A variety of factors, including the failure of mitochondrial dynamics and quality control, have made it extremely difficult to narrow down drug discovery targets. We have identified MITOL (mit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21113781
更新日期：2020-05-27 00:00:00
abstract：:Phosphoinositides play a crucial role in regulating many cellular functions, such as actin dynamics, signaling, intracellular trafficking, membrane dynamics, and cell-matrix adhesion. Central to this process is phosphatidylinositol bisphosphate (PIP2). The levels of PIP2 in the membrane are rapidly altered by the acti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21218342
更新日期：2020-11-06 00:00:00
abstract：:The phylum Thermotogae is composed of a single class (Thermotogae), 4 orders (Thermotogales, Kosmotogales, Petrotogales, Mesoaciditogales), 5 families (Thermatogaceae, Fervidobacteriaceae, Kosmotogaceae, Petrotogaceae, Mesoaciditogaceae), and 13 genera. They have been isolated from extremely hot environments whose cha...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms22010341
更新日期：2020-12-30 00:00:00
abstract：:This study was aimed to clarify the effect of honokiol (Hon) on the activity of Cytochrome P450 (CYP450) enzymes, and the level of mRNA expression of liver and kidney transporters in type 2 diabetic rats induced by high-fat diet and strepotozotocin. Rats were randomly divided into normal control (NC) group, diabetic c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19030815
更新日期：2018-03-12 00:00:00
abstract：:Atrial fibrillation (AF) is a type of sustained arrhythmia in humans often characterized by devastating alterations to the cardiac conduction system as well as the structure of the atria. AF can lead to decreased cardiac function, heart failure, and other complications. Long non-coding RNAs (lncRNAs) have been shown t...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21155424
更新日期：2020-07-30 00:00:00
abstract：:Multiple myeloma (MM) is a hematologic disorder of B lymphocytes characterized by the accumulation of malignant plasma cells (PCs) in the bone marrow. The altered plasma cells overproduce abnormal monoclonal immunoglobulins and also stimulate osteoclasts. The host's immune system and microenvironment are of paramount ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21145002
更新日期：2020-07-15 00:00:00
abstract：:Non-bullous congenital ichthyosiform erythroderma (NBCIE) is a hereditary disorder of keratinization caused by pathogenic variants in genes encoding enzymes important to lipid processing and terminal keratinocyte differentiation. Impaired function of these enzymes can cause pathologic epidermal scaling, significantly ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160921791
更新日期：2015-09-09 00:00:00
abstract：:Plant lipid transfer proteins (LTPs) are encoded by multigene families and play important roles in plant physiology. One full-length cDNA encoding an Arabidopsis LTP3 homologue was isolated from maize by RT-PCR and named as ZmLTP3. RT-PCR analysis indicated that the ZmLTP3 expression is induced by salicylic acid (SA),...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14035025
更新日期：2013-03-01 00:00:00
abstract：:Brown adipose tissue (BAT) is an important target for obesity treatment and prevention. Soluble epoxide hydrolase (sEH) converts bioactive epoxy fatty acids (EpFAs) into less active diols. sEH inhibitors (sEHI) are beneficial in many chronic diseases by stabilizing EpFAs. However, roles of sEH and sEHI in brown adipog...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197039
更新日期：2020-09-24 00:00:00
abstract：:Serum amyloid A (SAA) promotes endothelial inflammation and dysfunction that is associated with cardiovascular disease and renal pathologies. SAA is an apoprotein for high-density lipoprotein (HDL) and its sequestration to HDL diminishes SAA bioactivity. Herein we investigated the effect of co-supplementing HDL on SAA...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041316
更新日期：2020-02-15 00:00:00
abstract：:Mechanotransduction is a physiological process in which external mechanical stimulations are perceived, interpreted, and translated by cells into biochemical signals. Mechanical stimulations exerted by extracellular matrix stiffness and cell-cell contacts are continuously applied to living cells, thus representing a k...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249426
更新日期：2020-12-10 00:00:00
abstract：:Vascular inflammation plays a key role in the pathogenesis and progression of atherosclerosis, a main complication of diabetes. The present study investigated whether an aqueous extract of Portulaca oleracea (AP) prevents the TNF-α-induced vascular inflammatory process in the human umbilical vein endothelial cell (HUV...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13055628
更新日期：2012-01-01 00:00:00
abstract：:Forty mice were randomly divided into four groups on the basis of the diet to be fed as follows: 5% (low) fat diet (T1: LF); 20% (high) fat diet (T2: HF); 20% fat containing 1% conjugated linoleic acid (CLA) (T3: HFC); and 20% fat containing 1% CLA with 0.5% biopolymers (T4: HFCB). The high-fat with CLA diet groups (H...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14046848
更新日期：2013-03-26 00:00:00
abstract：:Human leukocyte antigen (HLA) class I ligands and Killer immunoglobulin-like receptors (KIRs) regulate the cytolytic activity of natural killer (NK) cells and certain T cells. We examined their genetic predisposition to disease susceptibility and clinical phenotypes in Taiwanese ankylosing spondylitis (AS) patients. K...
journal_title：International journal of molecular sciences
pub_type： 临床试验,杂志文章
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更新日期：2017-08-16 00:00:00
abstract：:Chalcones are precursors of flavonoid biosynthesis in plants. Both flavonoids and chalcones are intensively investigated because of a large spectrum of their biological activities. Among others, anticancer and antiangiogenic effects account for the research interest of these substances. Because of an essential role in...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19010027
更新日期：2017-12-22 00:00:00
abstract：:The binding of epidermal growth factor (EGF) to EGF receptor (EGFR) stimulates cell mitogenesis and survival through various signalling cascades. EGF also stimulates rapid EGFR endocytosis and its eventual degradation in lysosomes. The immediate events induced by ligand binding include receptor dimerization, activatio...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17081200
更新日期：2016-07-25 00:00:00
abstract：:Intracranial malignancies, such as primary brain cancers and brain-localized metastases derived from peripheral cancers, are particularly difficult to treat with therapeutic agents, because the blood-brain barrier (BBB) effectively minimizes brain entry of the vast majority of agents arriving from the systemic circula...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19123905
更新日期：2018-12-06 00:00:00
