High-Density Lipoprotein (HDL) Inhibits Serum Amyloid A (SAA)-Induced Vascular and Renal Dysfunctions in Apolipoprotein E-Deficient Mice.


:Serum amyloid A (SAA) promotes endothelial inflammation and dysfunction that is associated with cardiovascular disease and renal pathologies. SAA is an apoprotein for high-density lipoprotein (HDL) and its sequestration to HDL diminishes SAA bioactivity. Herein we investigated the effect of co-supplementing HDL on SAA-mediated changes to vascular and renal function in apolipoprotein E-deficient (ApoE-/-) mice in the absence of a high-fat diet. Male ApoE-/- mice received recombinant human SAA or vehicle (control) by intraperitoneal (i.p.) injection every three days for two weeks with or without freshly isolated human HDL supplemented by intravenous (i.v.) injection in the two weeks preceding SAA stimulation. Aorta and kidney were harvested 4 or 18 weeks after commencement of treatment. At 4 weeks after commencement of treatment, SAA increased aortic vascular cell adhesion molecule (VCAM)-1 expression and F2-isoprostane level and decreased cyclic guanosine monophosphate (cGMP), consistent with SAA stimulating endothelial dysfunction and promoting atherosclerosis. SAA also stimulated renal injury and inflammation that manifested as increased urinary protein, kidney injury molecule (KIM)-1, and renal tissue cytokine/chemokine levels as well as increased protein tyrosine chlorination and P38 MAPkinase activation and decreased in Bowman's space, confirming that SAA elicited a pro-inflammatory phenotype in the kidney. At 18 weeks, vascular lesions increased significantly in the cohort of ApoE-/- mice treated with SAA alone. By contrast, pretreatment of mice with HDL decreased SAA pro-inflammatory activity, inhibited SAA enhancement of aortic lesion size and renal function, and prevented changes to glomerular Bowman's space. Taken together, these data indicate that supplemented HDL reduces SAA-mediated endothelial and renal dysfunction in an atherosclerosis-prone mouse model.


Int J Mol Sci


Cai X,Ahmad G,Hossain F,Liu Y,Wang X,Dennis J,Freedman B,Witting PK




Has Abstract


2020-02-15 00:00:00










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    journal_title:International journal of molecular sciences

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    journal_title:International journal of molecular sciences

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    journal_title:International journal of molecular sciences

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    journal_title:International journal of molecular sciences

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    pub_type: 杂志文章


    authors: Vermeulen M,Del Vento F,Kanbar M,Pyr Dit Ruys S,Vertommen D,Poels J,Wyns C

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sallam NA,Laher I

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Sánchez-Melgar A,Albasanz JL,Pallàs M,Martín M

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章


    authors: Znalesniak EB,Fu T,Salm F,Händel U,Hoffmann W

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Wang C,Wang L,Ding Y,Lu X,Zhang G,Yang J,Zheng H,Wang H,Jiang Y,Xu L

    更新日期:2017-12-08 00:00:00

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    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Garajová I,Ferracin M,Porcellini E,Palloni A,Abbati F,Biasco G,Brandi G

    更新日期:2017-07-17 00:00:00

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    abstract::Metastasis to the central nervous system remains difficult to treat, and such patients are faced with a dismal prognosis. The blood-brain barrier (BBB), despite being partially compromised within malignant lesions in the brain, still retains much of its barrier function and prevents most chemotherapeutic agents from e...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审


    authors: Chen TC,Da Fonseca CO,Schönthal AH

    更新日期:2016-09-02 00:00:00