Abstract：
:Recombinant human fibroblast growth factor 10 (rhFGF-10) is frequently used to treat patients with skin injuries. It can also promote hair growth. However, the effective application of rhFGF-10 is limited because of its poor stability and transdermal absorption. In this study, polymerase chain reaction (PCR) and Southern blotting were used to identify transgenic safflowers carrying a gene encoding an oleosin-rhFGF-10 fusion protein. The size and structural integrity of oleosin-rhFGF-10 in oil bodies extracted from transgenic safflower seeds was characterized by polyacrylamide gel electrophoresis and western blotting. Oil body extracts containing oleosin-rhFGF-10 were topically applied to mouse skin. The absorption of oleosin-rhFGF-10 was studied by immunohistochemistry. Its efficiency in promoting wound healing and hair regeneration were evaluated in full thickness wounds and hair growth assays. We identified a safflower line that carried the transgene and expressed a 45 kDa oleosin-rhFGF-10 protein. Oil body-bound oleosin-rhFGF-10 was absorbed by the skin with higher efficiency and speed compared with prokaryotically-expressed rhFGF-10. Oleosin-rhFGF-10 also enhanced wound closure and promoted hair growth better than rhFGF-10. The application of oleosin-rhFGF-10 in oil bodies promoted its delivery through the skin, providing a basis for improved therapeutic effects in enhancing wound healing and hair growth.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Li W,Yang J,Cai J,Wang H,Tian H,Huang J,Qiang W,Zhang L,Li H,Li X,Jiang Cdoi
10.3390/ijms18102177subject
Has Abstractpub_date
2017-10-18 00:00:00issue
10issn
1422-0067pii
ijms18102177journal_volume
18pub_type
杂志文章abstract：:Merging targeted systemic gene delivery and systemic chemotherapy against cancer, chemovirotherapy, has the potential to improve chemotherapy and gene therapy treatments and overcome cancer resistance. We introduced a bacteriophage (phage) vector, named human adeno-associated virus (AAV)/phage or AAVP, for the systemi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2017-07-07 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-04-27 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2015-09-25 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2017-03-28 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2013-11-14 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-05-25 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-11-15 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2013-01-25 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2020-08-11 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2017-08-18 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-09-10 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2020-07-17 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2014-05-26 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2014-10-20 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2017-11-06 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2012-11-08 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2020-01-16 00:00:00
abstract：:Sunflower (Helianthus annuus L.) oil remains under-utilised albeit one of the major seed oils produced world-wide. Moreover, the high oleic sunflower varieties make the oil attractive for applications requiring high temperature processes and those targeting the C=C double bond functionality. Herein an overview of the ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2008-08-01 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2012-01-01 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2014-08-21 00:00:00
abstract：:Intra-articular injection of platelet-rich plasma (PRP) has been established as a suitable treatment for knee osteoarthritis. Here, we present a double-blind randomized controlled clinical trial, conducted in a public Hospital of the Spanish National Health Care System, to evaluate the efficacy of injecting autologous...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.3390/ijms17071064
更新日期：2016-07-02 00:00:00
