Abstract：
:Biologic scaffolds composed of extracellular matrix components have been proposed to repair and reconstruct a variety of tissues in clinical and pre-clinical studies. Injectable gels can fill and conform any three-dimensional shape and can be delivered to sites of interest by minimally invasive techniques. In this study, a biological gel was produced from a decellularized porcine urinary bladder by enzymatic digestion with pepsin. The enzymatic digestion was confirmed by visual inspection after dissolution in phosphate-buffered saline solution and Fourier-transform infrared spectroscopy. The rheological and biological properties of the gel were characterized and compared to those of the MatrigelTM chosen as a reference material. The storage modulus G' reached 19.4 ± 3.7 Pa for the 30 mg/mL digested decellularized bladder gels after ca. 3 h at 37 °C. The results show that the gel formed of the porcine urinary bladder favored the spontaneous differentiation of human and rabbit adipose-derived stem cells in vitro into smooth muscle cells to the detriment of cell proliferation. The results support the potential of the developed injectable gel for tissue engineering applications to reconstruct for instance the detrusor muscle part of the human urinary bladder.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Moreno-Manzano V,Zaytseva-Zotova D,López-Mocholí E,Briz-Redón Á,Løkensgard Strand B,Serrano-Aroca Ádoi
10.3390/ijms21228608subject
Has Abstractpub_date
2020-11-15 00:00:00issue
22issn
1422-0067pii
ijms21228608journal_volume
21pub_type
杂志文章abstract：:Epidemiological results revealed that there is an inverse correlation between high-density lipoprotein (HDL) cholesterol levels and risks of atherosclerotic cardiovascular disease (ASCVD). Mounting evidence supports that HDLs are atheroprotective, therefore, many therapeutic approaches have been developed to increase ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21030739
更新日期：2020-01-23 00:00:00
abstract：:Colorectal cancer (CRC) is one of the most common malignant diseases worldwide, but the involved signaling pathways and driven-genes are largely unclear. This study integrated four cohorts profile datasets to elucidate the potential key candidate genes and pathways in CRC. Expression profiles GSE28000, GSE21815, GSE44...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18040722
更新日期：2017-03-28 00:00:00
abstract：:At present, studies on macrophage proteins mainly focus on biological stimuli, with less attention paid to the responses of macrophage proteins to physical stimuli, such as electric fields. Here, we exploited the electric field-sensitive hub proteins of macrophages. RAW 264.7 macrophages were treated with a direct cur...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21124505
更新日期：2020-06-24 00:00:00
abstract：:Drug delivery nanocarriers, especially targeted drug delivery by liposomes are emerging as a class of therapeutics for cancer. Early research results suggest that liposomal therapeutics enhanced efficacy, while simultaneously reducing side effects, owing to properties such as more targeted localization in tumors and a...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms16010230
更新日期：2014-12-24 00:00:00
abstract：:Neurodegenerative disorders affect around one billion people worldwide. They can arise from a combination of genomic, epigenomic, metabolic, and environmental factors. Aging is the leading risk factor for most chronic illnesses of old age, including Alzheimer's and Parkinson's diseases. A progressive neurodegenerative...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20030719
更新日期：2019-02-08 00:00:00
abstract：:Non-coding RNA species contribute more than 90% of all transcripts and have gained increasing attention in the last decade. One of the most recent members of this group are long non-coding RNAs (lncRNAs) which are characterized by a length of more than 200 nucleotides and a lack of coding potential. However, in contra...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms20133276
更新日期：2019-07-03 00:00:00
abstract：:Endometriosis is characterized by the presence of endometrial tissue outside the uterus. While endometriotic tissue is commonly localized in the pelvic cavity, it can also be found in distant sites, including the brain. The origin and pathophysiology of tissue migration is poorly understood; retrograde menstruation is...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20153740
更新日期：2019-07-31 00:00:00
abstract：:The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17101719
更新日期：2016-10-14 00:00:00
abstract：:Astaxanthin, a potent antioxidant carotenoid, plays a major role in modulating the immune response. In this study, we examined the immunomodulatory effects of astaxanthin on cytokine production in primary cultured lymphocytes both in vitro and ex vivo. Direct administration of astaxanthin (70-300 nM) did not produce c...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms17010044
更新日期：2015-12-29 00:00:00
abstract：:The concept of regenerating tissues, with properties and functions that mimic natural tissues, has attracted significant attention in recent years. [...]. ...
journal_title：International journal of molecular sciences
pub_type： 社论
doi：10.3390/ijms19123960
更新日期：2018-12-09 00:00:00
abstract：:Several studies show that triple-negative breast cancer (TNBC) patients have the lowest vitamin D concentration among all breast cancer types, suggesting that this vitamin may induce a protective effect against TNBC. This effect of the active metabolite of vitamin D, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D), can be attr...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21103670
更新日期：2020-05-23 00:00:00
abstract：:DNA alkylating drugs have been used in clinics for more than seventy years. The diversity of their mechanism of action (major/minor groove; mono-/bis-alkylation; intra-/inter-strand crosslinks; DNA stabilization/destabilization, etc.) has undoubtedly major consequences on the cellular response to treatment. The aim of...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms161125971
更新日期：2015-11-05 00:00:00
abstract：:Idiopathic pulmonary fibrosis (IPF) is a chronic disease characterized by fibroblasts activation, ECM accumulation, and diffused alveolar inflammation. The role of inflammation in IPF is still controversial and its involvement may follow nontraditional mechanisms. It is seen that a pathological microenvironment may af...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218140
更新日期：2020-10-30 00:00:00
abstract：:Chondrocyte dysfunction occurs during the development of osteoarthritis (OA), typically resulting from a deleterious increase in oxidative stress. Accordingly, strategies for arresting oxidative stress-induced chondrocyte dysfunction may lead to new potential therapeutic targets for OA treatment. Forkhead box O (FoxO)...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms19123794
更新日期：2018-11-28 00:00:00
abstract：:In this paper, nanofibrous membranes based on chitosan (CS), poly (vinyl alcohol) (PVA) and graphene oxide (GO) composites, loaded with antibiotic drugs, such as Ciprofloxacin (Cip) and Ciprofloxacin hydrochloride (CipHcl) were prepared via the electrospinning technique. The uniform and defect-free CS/PVA nanofibers w...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184395
更新日期：2019-09-06 00:00:00
abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most frequent human enzymopathy, affecting over 400 million people globally. Worldwide, 217 mutations have been reported at the genetic level, and only 19 have been found in Mexico. The objective of this work was to contribute to the knowledge of the function ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21082732
更新日期：2020-04-15 00:00:00
abstract：:The number of diabetes mellitus (DM) patients is increasing, and stroke is deeply associated with DM. Recently, neuroprotective effects of glucagon-like peptide-1 (GLP-1) are reported. In this study, we explored whether liraglutide, a GLP-1 analogue exerts therapeutic effects on a rat stroke model. Wistar rats receive...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms141121513
更新日期：2013-10-30 00:00:00
abstract：:3,3'-Diindolylmethane (DIM) is a bioactive compound found in Cruciferous vegetables that possesses health benefits such as antioxidant, anticancer, and anti-inflammatory effects. However, hydrophobicity and photolabile limit its pharmaceutical applications. This study aims to prepare and characterize DIM-encapsulated ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20163917
更新日期：2019-08-12 00:00:00
abstract：:A T-maze test is an experimental approach that is used in congenital research. However, the food reward-based protocol for the T-maze test in fish has low efficiency and a long training period. The aim of this study is to facilitate the T-maze conditions by using a combination of the principles of passive avoidance an...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21041464
更新日期：2020-02-21 00:00:00
abstract：:Our previous study showed that a single lipopolysaccharide (LPS) treatment to neonatal rats could induce a long-lasting neuroinflammatory response and dopaminergic system injury late in life. This is evidenced by a sustained activation of microglia and elevated interleukin-1β (IL-1β) levels, as well as reduced tyrosin...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms16048635
更新日期：2015-04-17 00:00:00
abstract：:Brain metastases (BM) from colorectal cancer (CRC) are a rare but increasing event. Surgical resection of oligometastatic disease, including BM, may produce a survival benefit in selected patients. Previous studies described the HER-2 expression patterns in CRC patients, but its prognostic role still remains controver...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14022370
更新日期：2013-01-24 00:00:00
abstract：:Mechanotransduction is a physiological process in which external mechanical stimulations are perceived, interpreted, and translated by cells into biochemical signals. Mechanical stimulations exerted by extracellular matrix stiffness and cell-cell contacts are continuously applied to living cells, thus representing a k...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249426
更新日期：2020-12-10 00:00:00
abstract：:The aging kidney is a marked by a number of structural and functional changes, including an increased susceptibility to acute kidney injury (AKI). Previous studies from our laboratory have shown that aging male Fischer 344 rats (24 month) are more susceptible to apoptosis-mediated injury than young counterparts. In th...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020368
更新日期：2017-02-10 00:00:00
abstract：:Adiponectin is the adipokine associated with insulin sensitization, reducing liver gluconeogenesis, and increasing fatty acid oxidation and glucose uptake. Adiponectin is present in the kidneys, mainly in the arterial endothelium and smooth muscle cells, as well as in the capillary endothelium, and might be considered...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms21249375
更新日期：2020-12-09 00:00:00
abstract：:Internal tandem duplication of FLT3 juxtamembrane domain (FLT3-ITD)-positive acute myeloid leukemia (AML) leads to poor clinical outcomes after chemotherapy. We aimed to establish a cytarabine-resistant line from FLT3-ITD-positive MV4-11 (MV4-11-P) cells and examine the development of resistance. The FLT3-ITD mutation...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20051230
更新日期：2019-03-11 00:00:00
abstract：:Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21134774
更新日期：2020-07-05 00:00:00
abstract：:Fe3O4-Au hybrid nanoparticles (HNPs) have shown increasing potential for biomedical applications such as image guided stimuli responsive drug delivery. Incorporation of the unique properties of HNPs into thermally responsive scaffolds holds great potential for future biomedical applications. Here we successfully fabri...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14048585
更新日期：2013-04-19 00:00:00
abstract：:Curcumin diglutaric acid (CurDG), an ester prodrug of curcumin, has the potential to be developed as an anti-inflammatory agent due to its improved solubility and stability. In this study, the anti-inflammatory effects of CurDG were evaluated. The effects of CurDG on inflammatory mediators were evaluated in LPS-stimul...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21165700
更新日期：2020-08-09 00:00:00
abstract：:Both genetic and epigenetic alterations can control the progression of cancer. Genetic alterations are impossible to reverse, while epigenetic alterations are reversible. This advantage suggests that epigenetic modifications should be preferred in therapy applications. DNA methyltransferases and histone deacetylases h...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
doi：10.3390/ijms12074465
更新日期：2011-01-01 00:00:00
abstract：:Animal venoms are small natural mixtures highly enriched in bioactive components. They are known to target at least two important pharmacological classes of cell surface receptors: ion channels and G protein coupled receptors. Since sperm cells express a wide variety of ion channels and membrane receptors, required fo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21207786
更新日期：2020-10-21 00:00:00
