Abstract:
:tRNA and some other non-coding RNA genes are transcribed by RNA polymerase III (pol III), due to the presence of intragenic promoter, consisting of boxes A and B spaced by 30-40 bp. Such pol III promoters, called type 2, are also intrinsic to Short Interspersed Elements (SINEs). The contribution of 5'-flanking sequences to the transcription efficiency of genes containing type 2 promoters is still studied insufficiently. Here, we studied this issue, focusing on the genes of two small non-coding RNAs (4.5SH and 4.5SI), as well as B1 and B2 SINEs from the mouse genome. We found that the regions from position -31 to -24 may significantly influence the transcription of genes and SINEs. We studied the influence of nucleotide substitutions in these sites, representing TATA-like boxes, on transcription of 4.5SH and 4.5SI RNA genes. As a rule, the substitutions of A and T to G or C reduced the transcription level, although the replacement of C with A also lowered it. In 4.5SH gene, five distal nucleotides of -31/-24 box (TTCAAGTA) appeared to be the most important, while in the box -31/-24 of 4.5SI gene (CTACATGA), all nucleotides, except for the first one, contributed significantly to the transcription efficiency. Random sequences occurring at positions -31/-24 upstream of SINE copies integrated into genome, promoted their transcription with different efficacy. In the 5'-flanking sequences of 4.5SH and 4.5SI RNA genes, the recognition sites of CREB, C/EBP, and Sp1 factors were found, and their deletion decreased the transcription.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Tatosyan KA,Stasenko DV,Koval AP,Gogolevskaya IK,Kramerov DAdoi
10.3390/ijms21103706subject
Has Abstractpub_date
2020-05-25 00:00:00issue
10issn
1422-0067pii
ijms21103706journal_volume
21pub_type
杂志文章abstract::Cryopreservation of immature testicular tissue (ITT) prior to chemo/radiotherapy is now ethically accepted and is currently the only way to preserve fertility of prepubertal boys about to undergo cancer therapies. So far, three-dimensional culture of testicular cells isolated from prepubertal human testicular tissue w...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215476
更新日期:2019-11-03 00:00:00
abstract::Recent studies have shown that free fatty acids are associated with chronic inflammation, which may be involved in vascular injury. The intake of eicosapentaenoic acid (EPA) can decrease cardiovascular disease risks, but the protective mechanisms of EPA on endothelial cells remain unclear. In this study, primary human...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150610334
更新日期:2014-06-10 00:00:00
abstract::Neurodegenerative diseases may have distinct genetic etiologies and pathological manifestations, yet share common cellular mechanisms underpinning neuronal damage and dysfunction. These cellular mechanisms include excitotoxicity, calcium dysregulation, oxidative damage, ER stress and neuroinflammation. Recent data hav...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17081332
更新日期:2016-08-12 00:00:00
abstract::Metastatic melanoma is the most deadly type of skin cancer. Despite the success of immunotherapy and targeted agents, the majority of patients experience disease recurrence upon treatment and die due to their disease. Long non-coding RNAs (lncRNAs) are a new subclass of non-protein coding RNAs involved in (epigenetic)...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18040715
更新日期:2017-03-28 00:00:00
abstract::Skin cancer is the most common type of cancer worldwide. Ozone depletion and climate changes might cause a further increase in the incidence rate in the future. Although the early detection of skin cancer enables it to be treated successfully, some tumours can evolve and become more aggressive, especially in the case ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20225781
更新日期:2019-11-17 00:00:00
abstract::The presence of lysine (Lys) or arginine (Arg) and tryptophan (Trp) are important for the antimicrobial effects of cationic peptides. Therefore, we designed and synthesized a series of antimicrobial peptides with various numbers of Lys (or Arg) and Trp repeats [(KW and RW)(n)-NH(2), where n equals 2, 3, 4, or 5]. Anti...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms131115042
更新日期:2012-11-15 00:00:00
abstract::Ly-6/uPAR or three-finger proteins (TFPs) contain a disulfide-stabilized β-structural core and three protruding loops (fingers). In mammals, TFPs have been found in epithelium and the nervous, endocrine, reproductive, and immune systems. Here, using heteronuclear NMR, we determined the three-dimensional (3D) structure...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197280
更新日期:2020-10-01 00:00:00
abstract::Cultured human primary cells have a limited lifespan undergoing dedifferentiation or senescence. Anterior cruciate ligaments (ACL) are hypocellular but tissue engineering (TE) requires high cell numbers. Simian virus (SV) 40 tumor (T) antigen expression could extend the lifespan of cells. This study aimed to identify ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21020593
更新日期:2020-01-16 00:00:00
abstract::The melanin-inducing properties of cirsimaritin were investigated in murine B16F10 cells. Cirsimaritin is an active flavone with methoxy groups, which is isolated from the branches of Lithocarpus dealbatus. Tyrosinase activity and melanin content in murine B16F10 melanoma cells were increased by cirsimaritin in a dose...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16048772
更新日期:2015-04-20 00:00:00
abstract::DNA G-quadruplexes (G4s) form in relevant genomic regions and intervene in several biological processes, including the modulation of oncogenes expression, and are potential anticancer drug targets. The human KRAS proto-oncogene promoter region contains guanine-rich sequences able to fold into G4 structures. Here, by u...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010448
更新日期:2021-01-05 00:00:00
abstract::The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17101719
更新日期:2016-10-14 00:00:00
abstract::The effective dose of irradiation to control pest mites in quarantine has been studied extensively, but the molecular mechanisms underlying the effects of the irradiation on mites are largely unknown. In this study, exposure to 400 Gy of γ rays had significant (p < 0.05) effects on the adult survival, fecundity and eg...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms161126004
更新日期:2015-11-11 00:00:00
abstract::G protein-coupled receptors (GPCRs) are a large superfamily of cell-surface signaling proteins that bind extracellular ligands and transduce signals into cells via heterotrimeric G proteins. GPCRs are highly tractable drug targets. Aberrant expression of GPCRs and G proteins has been observed in various cancers and th...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17050707
更新日期:2016-05-11 00:00:00
abstract::Neurodegenerative diseases (NDDs) such as Alzheimer's disease, Parkinson's disease and Huntington's disease (HD), amyotrophic lateral sclerosis, and prion diseases are all characterized by the accumulation of protein aggregates (amyloids) into inclusions and/or plaques. The ubiquitous presence of amyloids in NDDs sugg...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19010325
更新日期:2018-01-22 00:00:00
abstract::Chronic liver disease is generally widespread, and a test for screening fibrotic subjects in a large population is needed. The ability of Wisteria floribunda agglutinin-positive mac-2 binding protein (WFA+-M2BP) to detect significant fibrosis was investigated in health checkup subjects in this research. Of 2021 health...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010040
更新日期:2020-12-22 00:00:00
abstract::Oil palm is an oil-producing crop of major importance at the global scale. Oil palm mesocarp lipids are used for myriads industrial applications, and market demand has been growing for decades. In addition, oil palm seeds are oleaginous, and the oil extracted therefrom can be used for several purposes, from food to co...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21124227
更新日期:2020-06-13 00:00:00
abstract::Magnetic resonance spectroscopy (MRS) is a non-invasive method for quantitative estimation of liver fat. Knowledge of its imprecision, which comprises biological variability and measurement error, is required to design therapeutic trials with measurement of change. The role of adipocyte lipolysis in ectopic fat accumu...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17071089
更新日期:2016-07-08 00:00:00
abstract::Yeonsan Ogye is a rare Korean domestic chicken breed whose entire body, including feathers and skin, has a unique black coloring. Although some protein-coding genes related to this unique feature have been examined, non-coding elements have not been widely investigated. Thus, we evaluated coding and non-coding transcr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19082359
更新日期:2018-08-10 00:00:00
abstract::Doxorubicin (DOX) is an anthracycline drug with a wide spectrum of antineoplastic activities. However, it causes cardiac cytotoxicity, and this limits its clinical applications. MicroRNA-21 (miR-21) plays a vital role in regulating cell proliferation and apoptosis. While miR-21 is preferentially expressed in adult car...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160714511
更新日期:2015-06-26 00:00:00
abstract::Monocyte chemoattractant protein-1 (MCP-1) is a well-known chemokine critically involved in the pathophysiological progression of several inflammatory diseases including arthrosclerosis. N-caffeoyltryptamine is a phenolic amide with strong anti-inflammatory effects. Therefore, in this paper, the potential effect of N-...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18061148
更新日期:2017-05-27 00:00:00
abstract::Since the European Union (EU) announced their animal testing ban in 2013, all animal experiments related to cosmetics have been prohibited, creating a demand for alternatives to animal experiments for skin studies. Here, we investigated whether an ex vivo live porcine skin model can be employed to study the safety and...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22020657
更新日期:2021-01-11 00:00:00
abstract::The growing trend for women to postpone childbearing has resulted in a dramatic increase in the incidence of aneuploid pregnancies. Despite the importance to human reproductive health, the events precipitating female age-related meiotic errors are poorly understood. To gain new insight into the molecular basis of age-...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21061934
更新日期:2020-03-12 00:00:00
abstract::Mitochondria are complex organelles that play critical roles in diverse aspects of cellular function. Heart disease and a number of other pathologies are associated with perturbations in the molecular machinery of the mitochondria. Therefore, comprehensive, unbiased examination of the mitochondrial proteome represents...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15069285
更新日期:2014-05-26 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a fatal, rapidly progressing neurodegenerative disease affecting motor neurons, and frontotemporal dementia (FTD) is a behavioural disorder resulting in early-onset dementia. Hexanucleotide (G4C2) repeat expansions in the gene encoding chromosome 9 open reading frame 72 (C9orf72)...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19103137
更新日期:2018-10-12 00:00:00
abstract::Lung cancer is the leading cause of death for malignancy worldwide. Its molecular profiling has enriched our understanding of cancer initiation and progression and has become fundamental to provide guidance on treatment with targeted therapies. Testing the presence of driver mutations in specific genes in lung tumors ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22020612
更新日期:2021-01-09 00:00:00
abstract::Chlorogenic acid (CGA) decreases colon cancer-cell proliferation but the combined anti-cancer effects of CGA with its major colonic microbial metabolites, caffeic acid (CA), 3-phenylpropionic acid (3-PPA) and benzoic acid (BA), needs elucidation as they occur together in colonic digesta. Caco-2 cancer cells were treat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030723
更新日期:2018-03-03 00:00:00
abstract::A series of perylene diimide (PDI) derivatives have been investigated at the CAM-B3LYP/6-31G(d) and the TD-B3LYP/6-31+G(d,p) levels to design solar cell acceptors with high performance in areas such as suitable frontier molecular orbital (FMO) energies to match oligo(thienylenevinylene) derivatives and improved charge...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17050721
更新日期:2016-05-13 00:00:00
abstract::Kidney transplant recipients frequently suffer from skin infections and malignancies, possibly due to the effects of long-term immunosuppressive therapy. While the relationships between immunosuppression and these pathological conditions have been widely investigated, little is known about the relative incidence and c...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17081362
更新日期:2016-08-19 00:00:00
abstract::The TEAD family of transcription factors is necessary for developmental processes. The family members contain a TEA domain for the binding with DNA elements and a transactivation domain for the interaction with transcription coactivators. TEAD proteins are required for the participation of coactivators to transmit the...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms17010138
更新日期:2016-01-21 00:00:00
abstract::Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, has five genotypes (I, II, III, IV, and V). JEV genotype I circulates widely in some Asian countries. However, current JEV vaccines based on genotype III strains show low neutralizing capacities against genotype I variants. In addition, JE has no specific...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17091386
更新日期:2016-08-24 00:00:00