Chromosome Missegregation in Single Human Oocytes Is Related to the Age and Gene Expression Profile.

Abstract:

:The growing trend for women to postpone childbearing has resulted in a dramatic increase in the incidence of aneuploid pregnancies. Despite the importance to human reproductive health, the events precipitating female age-related meiotic errors are poorly understood. To gain new insight into the molecular basis of age-related chromosome missegregation in human oocytes, we combined the transcriptome profiles of twenty single oocytes (derived from females divided into two groups according to age <35 and ≥35 years) with their chromosome status obtained by array comparative genomic hybridization (aCGH). Furthermore, we compared the transcription profile of the single oocyte with the surrounding cumulus cells (CCs). RNA-seq data showed differences in gene expression between young and old oocytes. Dysregulated genes play a role in important biological processes such as gene transcription regulation, cytoskeleton organization, pathways related to RNA maturation and translation. The comparison of the transcription profile of the oocyte and the corresponding CCs highlighted the differential expression of genes belonging to the G protein-coupled receptor superfamily. Finally, we detected the loss of a X chromosome in two oocytes derived from women belonging to the ≥35 years age group. These aneuploidies may be caused by the detriment of REEP4, an endoplasmic reticulum protein, in women aged ≥35 years. Here we gained new insight into the complex regulatory circuit between the oocyte and the surrounding CCs and uncovered a new putative molecular basis of age-related chromosome missegregation in human oocytes.

journal_name

Int J Mol Sci

authors

Barone S,Sarogni P,Valli R,Pallotta MM,Silvia G,Frattini A,Khan AW,Rapalini E,Parri C,Musio A

doi

10.3390/ijms21061934

subject

Has Abstract

pub_date

2020-03-12 00:00:00

issue

6

issn

1422-0067

pii

ijms21061934

journal_volume

21

pub_type

杂志文章
  • Innovative Therapeutic Strategies for Effective Treatment of Brain Metastases.

    abstract::Brain metastases are the most prevalent of intracranial malignancies. They are associated with a very poor prognosis and near 100% mortality. This has been the case for decades, largely because we lack effective therapeutics to augment surgery and radiotherapy. Notwithstanding improvements in the precision and efficac...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20061280

    authors: Lim M,Puttick S,Houston ZH,Thurecht KJ,Kalita-de Croft P,Mahler S,Rose SE,Jeffree RL,Mazzieri R,Dolcetti R,Lakhani SR,Saunus JM

    更新日期:2019-03-14 00:00:00

  • At the Crossroads of Clinical and Preclinical Research for Muscular Dystrophy-Are We Closer to Effective Treatment for Patients?

    abstract::Among diseases affecting skeletal muscle, muscular dystrophy is one of the most devastating and complex disorders. The term 'muscular dystrophy' refers to a heterogeneous group of genetic diseases associated with a primary muscle defect that leads to progressive muscle wasting and consequent loss of muscle function. M...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19051490

    authors: Gawlik KI

    更新日期:2018-05-16 00:00:00

  • Elemental Ingredients in the Macrophage Cocktail: Role of ZIP8 in Host Response to Mycobacterium tuberculosis.

    abstract::Tuberculosis (TB) is a global epidemic caused by the infection of human macrophages with the world's most deadly single bacterial pathogen, Mycobacterium tuberculosis (M.tb). M.tb resides in a phagosomal niche within macrophages, where trace element concentrations impact the immune response, bacterial metal metabolism...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18112375

    authors: Pyle CJ,Azad AK,Papp AC,Sadee W,Knoell DL,Schlesinger LS

    更新日期:2017-11-09 00:00:00

  • Distribution of alpha thalassaemia gene variants in diverse ethnic populations in malaysia: data from the institute for medical research.

    abstract::Alpha thalassaemia is highly prevalent in the plural society of Malaysia and is a public health problem. Haematological and molecular data from 5016 unrelated patients referred from various hospitals to the Institute for Medical Research for α thalassaemia screening from 2007 to 2010 were retrieved. The aims of this r...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms140918599

    authors: Ahmad R,Saleem M,Aloysious NS,Yelumalai P,Mohamed N,Hassan S

    更新日期:2013-09-10 00:00:00

  • Proteolytic Regulation of Parathyroid Hormone-Related Protein: Functional Implications for Skeletal Malignancy.

    abstract::Parathyroid hormone-related protein (PTHrP), with isoforms ranging from 139 to 173 amino acids, has long been implicated in the development and regulation of multiple tissues, including that of the skeleton, via paracrine and autocrine signaling. PTHrP is also known as a potent mediator of cancer-induced bone disease,...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20112814

    authors: Frieling JS,Lynch CC

    更新日期:2019-06-08 00:00:00

  • Mesenchymal Stem Cell Secretome as an Emerging Cell-Free Alternative for Improving Wound Repair.

    abstract::The use of mesenchymal stem cells (MSC) for the treatment of cutaneous wounds is currently of enormous interest. However, the broad translation of cell therapies into clinical use is hampered by their efficacy, safety, manufacturing and cost. MSCs release a broad repertoire of trophic factors and immunomodulatory cyto...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21197038

    authors: Ahangar P,Mills SJ,Cowin AJ

    更新日期:2020-09-24 00:00:00

  • Frizzled Receptors as Potential Therapeutic Targets in Human Cancers.

    abstract::Frizzled receptors (FZDs) are a family of seven-span transmembrane receptors with hallmarks of G protein-coupled receptors (GPCRs) that serve as receptors for secreted Wingless-type (WNT) ligands in the WNT signaling pathway. Functionally, FZDs play crucial roles in regulating cell polarity, embryonic development, cel...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms19051543

    authors: Zeng CM,Chen Z,Fu L

    更新日期:2018-05-22 00:00:00

  • Circadian Clock, Cell Division, and Cancer: From Molecules to Organism.

    abstract::As a response to environmental changes driven by the Earth's axial rotation, most organisms evolved an internal biological timer-the so called circadian clock-which regulates physiology and behavior in a rhythmic fashion. Emerging evidence suggests an intimate interplay between the circadian clock and another fundamen...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18040873

    authors: Shostak A

    更新日期:2017-04-20 00:00:00

  • Effects of Selective Substitution of Cysteine Residues on the Conformational Properties of Chlorotoxin Explored by Molecular Dynamics Simulations.

    abstract::Chlorotoxin (CTX) is a 36⁻amino acid peptide with eight Cys residues that forms four disulfide bonds. It has high affinity for the glioma-specific chloride channel and matrix metalloprotease-2. Structural and binding properties of CTX analogs with various Cys residue substitutions with l-α-aminobutyric acid (Abu) have...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20061261

    authors: Gregory AJ,Voit-Ostricki L,Lovas S,Watts CR

    更新日期:2019-03-13 00:00:00

  • Oxidative Stress Orchestrates MAPK and Nitric-Oxide Synthase Signal.

    abstract::Reactive oxygen species (ROS) are not only harmful to cell survival but also essential to cell signaling through cysteine-based redox switches. In fact, ROS triggers the potential activation of mitogen-activated protein kinases (MAPKs). The 90 kDa ribosomal S6 kinase 1 (RSK1), one of the downstream mediators of the MA...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21228750

    authors: Takata T,Araki S,Tsuchiya Y,Watanabe Y

    更新日期:2020-11-19 00:00:00

  • Biological activities of Toninia candida and Usnea barbata together with their norstictic acid and usnic acid constituents.

    abstract::The aim of this study was to investigate the chemical composition of acetone extracts of the lichens Toninia candida and Usnea barbata and in vitro antioxidant, antimicrobial, and anticancer activities of these extracts together with some of their major metabolites. The chemical composition of T. candida and U. barbat...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms131114707

    authors: Ranković B,Kosanić M,Stanojković T,Vasiljević P,Manojlović N

    更新日期:2012-11-12 00:00:00

  • The TEAD Family and Its Oncogenic Role in Promoting Tumorigenesis.

    abstract::The TEAD family of transcription factors is necessary for developmental processes. The family members contain a TEA domain for the binding with DNA elements and a transactivation domain for the interaction with transcription coactivators. TEAD proteins are required for the participation of coactivators to transmit the...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms17010138

    authors: Zhou Y,Huang T,Cheng AS,Yu J,Kang W,To KF

    更新日期:2016-01-21 00:00:00

  • Brassica Bioactives Could Ameliorate the Chronic Inflammatory Condition of Endometriosis.

    abstract::Endometriosis is a chronic, inflammatory, hormone-dependent disease characterized by histological lesions produced by the presence of endometrial tissue outside the uterine cavity. Despite the fact that an estimated 176 million women are affected worldwide by this gynecological disorder, risk factors that cause endome...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms21249397

    authors: García-Ibañez P,Yepes-Molina L,Ruiz-Alcaraz AJ,Martínez-Esparza M,Moreno DA,Carvajal M,García-Peñarrubia P

    更新日期:2020-12-10 00:00:00

  • Methionine Regulates mTORC1 via the T1R1/T1R3-PLCβ-Ca2+-ERK1/2 Signal Transduction Process in C2C12 Cells.

    abstract::The mammalian target of rapamycin complex 1 (mTORC1) integrates amino acid (AA) availability to support protein synthesis and cell growth. Taste receptor type 1 member (T1R) is a G protein-coupled receptor that functions as a direct sensor of extracellular AA availability to regulate mTORC1 through Ca2+ stimulation an...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms17101684

    authors: Zhou Y,Ren J,Song T,Peng J,Wei H

    更新日期:2016-10-11 00:00:00

  • Extracellular Spermine Activates DNA Methyltransferase 3A and 3B.

    abstract::We first demonstrated that long-term increased polyamine (spermine, spermidine, putrescine) intake elevated blood spermine levels in mice and humans, and lifelong consumption of polyamine-rich chow inhibited aging-associated increase in aberrant DNA methylation, inhibited aging-associated pathological changes, and ext...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20051254

    authors: Fukui T,Soda K,Takao K,Rikiyama T

    更新日期:2019-03-12 00:00:00

  • Optimizing a Male Reproductive Aging Mouse Model by D-Galactose Injection.

    abstract::The d-galactose (d-gal)-injected animal model, which is typically established by administering consecutive subcutaneous d-gal injections to animals for approximately six or eight weeks, has been frequently used for aging research. In addition, this animal model has been demonstrated to accelerate aging in the brain, k...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms17010098

    authors: Liao CH,Chen BH,Chiang HS,Chen CW,Chen MF,Ke CC,Wang YY,Lin WN,Wang CC,Lin YH

    更新日期:2016-01-13 00:00:00

  • The contribution of the elastic reaction is severely underestimated in studies on myofibril contraction.

    abstract::We have considered the Huxley-Simmons manoeuvre. On the assumption that the quick release is an elastic process and on the basis of the isometric tension and of the stiffness of the muscle fibre we calculated that the spontaneous release of the fibre requires approximately 43 micros, which is much faster than the obse...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms10030942

    authors: Grazi E,Pozzati S

    更新日期:2009-03-01 00:00:00

  • LHCSR Expression under HSP70/RBCS2 Promoter as a Strategy to Increase Productivity in Microalgae.

    abstract::Microalgae are unicellular photosynthetic organisms considered as potential alternative sources for biomass, biofuels or high value products. However, limited biomass productivity is commonly experienced in their cultivating system despite their high potential. One of the reasons for this limitation is the high therma...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms19010155

    authors: Perozeni F,Stella GR,Ballottari M

    更新日期:2018-01-05 00:00:00

  • The Interaction of the Senescent and Adjacent Breast Cancer Cells Promotes the Metastasis of Heterogeneous Breast Cancer Cells through Notch Signaling.

    abstract::Chemotherapy is one of the most common strategies for tumor treatment but often associated with post-therapy tumor recurrence. While chemotherapeutic drugs are known to induce tumor cell senescence, the roles and mechanisms of senescence in tumor recurrence remain unclear. In this study, we used doxorubicin to induce ...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms22020849

    authors: Zhang N,Ji J,Zhou D,Liu X,Zhang X,Liu Y,Xiang W,Wang M,Zhang L,Wang G,Huang B,Lu J,Zhang Y

    更新日期:2021-01-15 00:00:00

  • In Vitro Model for Hepatotoxicity Studies Based on Primary Human Hepatocyte Cultivation in a Perfused 3D Bioreactor System.

    abstract::Accurate prediction of the potential hepatotoxic nature of new pharmaceuticals remains highly challenging. Therefore, novel in vitro models with improved external validity are needed to investigate hepatic metabolism and timely identify any toxicity of drugs in humans. In this study, we examined the effects of diclofe...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms17040584

    authors: Knöspel F,Jacobs F,Freyer N,Damm G,De Bondt A,van den Wyngaert I,Snoeys J,Monshouwer M,Richter M,Strahl N,Seehofer D,Zeilinger K

    更新日期:2016-04-16 00:00:00

  • Extracellular Vesicle-Induced Classical Complement Activation Leads to Retinal Endothelial Cell Damage via MAC Deposition.

    abstract::Several studies have suggested that there is a link between membrane attack complex (MAC) deposition in the retina and the progression of diabetic retinopathy (DR). Our recent investigation demonstrated that circulating IgG-laden extracellular vesicles contribute to an increase in retinal vascular permeability in DR t...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21051693

    authors: Huang C,Fisher KP,Hammer SS,Busik JV

    更新日期:2020-03-01 00:00:00

  • The Contribution of Oxidative Stress and Inflamm-Aging in Human and Equine Asthma.

    abstract::Aging is associated with a dysregulation of the immune system, leading to a general pro-inflammatory state of the organism, a process that has been named inflamm-aging. Oxidative stress has an important role in aging and in the regulation of immune responses, probably playing a role in the development of age-related d...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18122612

    authors: Bullone M,Lavoie JP

    更新日期:2017-12-05 00:00:00

  • Phytochemicals Targeting Estrogen Receptors: Beneficial Rather Than Adverse Effects?

    abstract::In mammals, the effects of estrogen are mainly mediated by two different estrogen receptors, ERα and ERβ. These proteins are members of the nuclear receptor family, characterized by distinct structural and functional domains, and participate in the regulation of different biological processes, including cell growth, s...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms18071381

    authors: Lecomte S,Demay F,Ferrière F,Pakdel F

    更新日期:2017-06-28 00:00:00

  • Cisplatin Synergistically Enhances Antitumor Potency of Conditionally Replicating Adenovirus via p53 Dependent or Independent Pathways in Human Lung Carcinoma.

    abstract::Cisplatin is ranked as one of the most powerful and commonly prescribed anti-tumor chemotherapeutic agents which improve survival in many solid tumors including non-small cell lung cancer. However, the treatment of advanced lung cancer is restricted due to chemotherapy resistance. Here, we developed and investigated s...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20051125

    authors: Ali S,Tahir M,Khan AA,Chen XC,Ling M,Huang Y

    更新日期:2019-03-05 00:00:00

  • Chitosan Oligosaccharides Suppress Nuclear Factor-Kappa B Activation and Ameliorate Experimental Autoimmune Uveoretinitis in Mice.

    abstract::We investigated the therapeutic potential and mechanism of chitosan oligosaccharides (COS) for experimental autoimmune uveoretinitis (EAU) in mice. EAU was induced in C57/BL6 mice by injection of human interphotoreceptor retinoid-binding protein (IRBP) peptides. At the same time, a high or low dose (20 or 10 mg/kg) of...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms21218326

    authors: Hsu SM,Yang CH,Tsai HY,Lin CJ,Fang YH,Shieh CC,Chen SH

    更新日期:2020-11-06 00:00:00

  • The Direct Interaction between E93 and Kr-h1 Mediated Their Antagonistic Effect on Ovary Development of the Brown Planthopper.

    abstract::The juvenile hormone (JH) signalling and ecdysone signalling pathways are crucial endocrine signalling pathways that orchestrate the metamorphosis of insects. The metamorphic process, the morphological change from the immature to adult forms, is orchestrated by the dramatic reduction of JH and downstream transcription...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms20102431

    authors: Mao Y,Li Y,Gao H,Lin X

    更新日期:2019-05-16 00:00:00

  • Proteomic analysis of etiolated juvenile tetraploid Robinia pseudoacacia branches during different cutting periods.

    abstract::The propagation of hard-branch cuttings of tetraploid Robinia pseudoacacia (black locust) is restricted by the low rooting rate; however, etiolated juvenile tetraploid black locust branches result in a significantly higher rooting rate of cuttings compared with non-etiolated juvenile tetraploid branches. To identify p...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms15046674

    authors: Lu N,Xu Z,Meng B,Sun Y,Zhang J,Wang S,Li Y

    更新日期:2014-04-21 00:00:00

  • Physiological, Ultrastructural and Proteomic Responses in the Leaf of Maize Seedlings to Polyethylene Glycol-Stimulated Severe Water Deficiency.

    abstract::After maize seedlings grown in full-strength Hoagland solution for 20 days were exposed to 20% polyethylene glycol (PEG)-stimulated water deficiency for two days, plant height, shoot fresh and dry weights, and pigment contents significantly decreased, whereas malondialdehyde (MDA) content greatly increased. Using tran...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章

    doi:10.3390/ijms160921606

    authors: Shao R,Xin L,Mao J,Li L,Kang G,Yang Q

    更新日期:2015-09-08 00:00:00

  • Resveratrol and Its Effects on the Vascular System.

    abstract::Resveratrol, the phenolic substance isolated initially from Veratrum grandiflorum and richly present in grapes, wine, peanuts, soy, and berries, has been attracting attention of scientists and medical doctors for many decades. Herein, we review its effects on the vascular system. Studies utilizing cell cultures and pr...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20071523

    authors: Breuss JM,Atanasov AG,Uhrin P

    更新日期:2019-03-27 00:00:00

  • Mechanotransduction and Cytoskeleton Remodeling Shaping YAP1 in Gastric Tumorigenesis.

    abstract::The essential role of Hippo signaling pathway in cancer development has been elucidated by recent studies. In the gastrointestinal tissues, deregulation of the Hippo pathway is one of the most important driving events for tumorigenesis. It is widely known that Yes-associated protein 1 (YAP1) and WW domain that contain...

    journal_title:International journal of molecular sciences

    pub_type: 杂志文章,评审

    doi:10.3390/ijms20071576

    authors: Zhang J,Zhou Y,Tang PMK,Cheng ASL,Yu J,To KF,Kang W

    更新日期:2019-03-29 00:00:00