Abstract:
:Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, has five genotypes (I, II, III, IV, and V). JEV genotype I circulates widely in some Asian countries. However, current JEV vaccines based on genotype III strains show low neutralizing capacities against genotype I variants. In addition, JE has no specific treatment, except a few supportive treatments. Compound CW-33, an intermediate synthesized derivative of furoquinolines, was investigated for its antiviral activities against JEV in this study. CW-33 exhibited the less cytotoxicity to Syrian baby hamster kidney (BHK-21) and human medulloblastoma (TE761) cells. CW-33 dose-dependently reduced the cytopathic effect and apoptosis of JEV-infected cells. Supernatant virus yield assay pinpointed CW-33 as having potential anti-JEV activity with IC50 values ranging from 12.7 to 38.5 μM. Time-of-addition assay with CW-33 indicated that simultaneous and post-treatment had no plaque reduction activity, but continuous and simultaneous treatments proved to have highly effective antiviral activity, with IC50 values of 32.7 and 48.5 μM, respectively. CW-33 significantly moderated JEV-triggered Ca(2+) overload, which correlated with the recovery of mitochondria membrane potential as well as the activation of Akt/mTOR and Jak/STAT1 signals in treated infected cells. Phosphopeptide profiling by LC-MS/MS revealed that CW-33 upregulated proteins from the enzyme modulator category, such as protein phosphatase inhibitor 2 (I-2), Rho GTPase-activating protein 35, ARF GTPase-activating protein GIT2, and putative 3-phosphoinositide-dependent protein kinase 2. These enzyme modulators identified were associated with the activation of Akt/mTOR and Jak/STAT1 signals. Meanwhile, I-2 treatment substantially inhibited the apoptosis of JEV-infected cells. The results demonstrated that CW-33 exhibited a significant potential in the development of anti-JEV agents.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Huang SH,Lien JC,Chen CJ,Liu YC,Wang CY,Ping CF,Lin YF,Huang AC,Lin CWdoi
10.3390/ijms17091386subject
Has Abstractpub_date
2016-08-24 00:00:00issue
9issn
1422-0067pii
ijms17091386journal_volume
17pub_type
杂志文章abstract::Estrogen is believed to be pre-initiator in the risk of breast cancer. The BRCA1 is a tumor suppressor gene associated with breast and ovarian cancer risk. This report describes functional analysis of two BRCA1 missense mutations (Asp67Glu and Thr1051Ser) observed in the familial breast/ovarian cancer patients in Thai...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms10094187
更新日期:2009-09-24 00:00:00
abstract::The emergence of multidrug resistance is always associated with more rapid tumor recurrence and metastasis. P-glycoprotein (P-gp), which is a well-known multidrug-efflux transporter, confers enhanced invasion ability in drug-resistant cells. Previous studies have shown that P-gp probably exerts its tumor-promoting fun...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17101718
更新日期:2016-10-13 00:00:00
abstract::n/a. ...
journal_title:International journal of molecular sciences
pub_type: 已发布勘误
doi:10.3390/ijms18010178
更新日期:2017-01-17 00:00:00
abstract::Abnormalities in the melatonin signaling pathway and the involvement of melatonin receptor MT2 have been reported in patients with adolescent idiopathic scoliosis (AIS). Whether these abnormalities were involved in the systemic abnormal skeletal growth in AIS during the peripubertal period remain unknown. In this cros...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150917100
更新日期:2014-09-25 00:00:00
abstract::Increasing evidence demonstrates the importance of hippocampal neurogenesis, a fundamental mechanism of neuroplasticity associated with cognition and emotion, in correlation to neurodegenerative and psychiatric disorders. Neuropsychiatric disorders are often a result of chronic stress or pain followed by inflammation;...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18040739
更新日期:2017-03-31 00:00:00
abstract::The phase-transition temperatures of an elastin-like polypeptide (ELP) with the (GVGVP)40 sequence and solvent dipolarity/polarizability, hydrogen-bond donor acidity, and hydrogen-bond acceptor basicity in its aqueous solutions were quantified in the absence and presence of different salts (Na2SO4, NaCl, NaClO4, and N...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160613528
更新日期:2015-06-12 00:00:00
abstract::Wound healing is a complex process with a linear development that involves many actors in a multistep timeline commonly divided into four stages: Hemostasis, inflammation, proliferation, and remodeling. Chronic non-healing wounds fail to progress beyond the inflammatory phase, thus precluding the next steps and, ultim...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20153721
更新日期:2019-07-30 00:00:00
abstract::EAAC1 is important in modulating brain ischemic tolerance. Mice lacking EAAC1 exhibit increased susceptibility to neuronal oxidative stress in mice after transient cerebral ischemia. EAAC1 was first described as a glutamate transporter but later recognized to also function as a cysteine transporter in neurons. EAAC1-m...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms151119444
更新日期:2014-10-27 00:00:00
abstract::Nitrogen (N) is essential for plant growth and crop productivity. Organic N is a major form of remobilized N in plants' response to N limitation. It is necessary to understand the regulatory role of N limitation adaption (NLA) in organic N remobilization for this adaptive response. Transcriptional and proteomic analys...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21062171
更新日期:2020-03-21 00:00:00
abstract::Breast cancer is the most common cancer type among women worldwide. With breast cancer patients and survivors being reported to experience a repertoire of symptoms that are detrimental to their quality of life, the development of breast cancer treatment strategies that are effective with minimal side effects is theref...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18112423
更新日期:2017-11-15 00:00:00
abstract::Silibinin, an effective anti-cancer and chemopreventive agent in various epithelial cancer models, has been reported to inhibit cancer cell growth through mitogenic signaling pathways. However, whether it can inhibit human pancreatic carcinoma growth and what are the underlying mechanisms is still not well elucidated....
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms12084861
更新日期:2011-01-01 00:00:00
abstract::The dimension of the plants largest organelle-the vacuole-plays a major role in defining cellular elongation rates. The morphology of the vacuole is controlled by the actin cytoskeleton, but molecular players remain largely unknown. Recently, the Networked (NET) family of membrane-associated, actin-binding proteins ha...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20194752
更新日期:2019-09-25 00:00:00
abstract::(Pro)renin receptor ((P)RR) is a multi-functional molecule that is related to both the renin-angiotensin system (RAS) and vacuolar H⁺-ATPase (v-ATPase), an ATP-dependent multi-subunit proton pump. Soluble (P)RR (s(P)RR), which consists of the extracellular domain of (P)RR, is present in blood and urine. Elevated plasm...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18061313
更新日期:2017-06-20 00:00:00
abstract::Environmental or biomedical exposure to nanoparticles (NPs) can results in translocation and accumulation of NPs in the brain, which can lead to health-related problems. NPs have been shown to induce toxicity to neuronal cells through several direct mechanisms, but only a few studies have also explored the indirect ef...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21197030
更新日期:2020-09-24 00:00:00
abstract::The effects of acidic polysaccharides purified from Gastrodia rhizome on blood pressure and serum lipid levels in spontaneously hypertensive rats (SHR) fed a high-fat diet were investigated. Acidic polysaccharides were purified from crude polysaccharides by DEAE-Sepharose CL-6B. Thirty-six male SHR were randomly divid...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13010698
更新日期:2012-01-01 00:00:00
abstract::Chitosan-caseinate nanoparticles were synthesized by polyelectrolyte complex (PEC) formation. Caseinate is an anionic micellar nanocolloid in aqueous solutions, which association with the polycationic chitosan yielded polyelectrolyte complexes with caseinate cores surrounded by a chitosan corona. The pre-structuration...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21165602
更新日期:2020-08-05 00:00:00
abstract::Radish cytoplasmic male sterility (CMS) has been widely used for breeding in Raphanus and Brassica genera. However, the detailed regulation network of the male sterility remains to be determined. Our previous work has shown that the abnormalities in a CMS radish appeared shortly after the tetrad stage when microspores...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17010042
更新日期:2016-01-06 00:00:00
abstract::Transforming growth factor beta 3 (TGFβ3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGFβ3 absorbed in decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity of scaffold-associated TGFβ3 in an in vitro model. ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20215474
更新日期:2019-11-03 00:00:00
abstract::Alemtuzumab, a humanized monoclonal antibody targeting the surface molecule CD52, leads to a rapid depletion of immune cells in the innate and adaptive immune system. In phase 2 and 3 trials in multiple sclerosis (MS), infections have been reported more frequently in alemtuzumab than in interferon beta treated patient...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms160714669
更新日期:2015-06-29 00:00:00
abstract::Plants undergo several but very precise molecular, physiological, and biochemical modulations in response to biotic stresses. Mitogen-activated protein kinase (MAPK) cascades orchestrate multiple cellular processes including plant growth and development as well as plant responses against abiotic and biotic stresses. H...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20123023
更新日期:2019-06-20 00:00:00
abstract::Biofilms are a multicellular way of life, where bacterial cells are close together and embedded in a hydrated macromolecular matrix which offers a number of advantages to the cells. Extracellular polysaccharides play an important role in matrix setup and maintenance. A water-insoluble polysaccharide was isolated and p...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21051702
更新日期:2020-03-02 00:00:00
abstract::Microglia are first-line defense antigen-presenting phagocytes in the central nervous system. Activated microglial cells release pro-inflammatory cytokines and can trigger an oxidative burst. The amino acid glycine exerts anti-inflammatory, immunomodulatory and cytoprotective effects and influences cell volume regulat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21030804
更新日期:2020-01-26 00:00:00
abstract::The l-arginine/agmatine transporter AdiC is a prokaryotic member of the SLC7 family, which enables pathogenic enterobacteria to survive the extremely acidic gastric environment. Wild-type AdiC from Escherichia coli, as well as its previously reported point mutants N22A and S26A, were overexpressed homologously and pur...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19030918
更新日期:2018-03-20 00:00:00
abstract::Tea (Camellia sinensis (L.) O. Kuntze), one of the main crops in China, is high in various bioactive compounds including flavonoids, catechins, caffeine, theanine, and other amino acids. C. sinensis is also known as an accumulator of fluoride (F), and the bioactive compounds are affected by F, however, the mechanism r...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20020237
更新日期:2019-01-09 00:00:00
abstract::The glycoprotein P-selectin belongs to the selectin family of cell adhesion molecules. In this study, we cloned the full-length cDNA of P-selectin from zebrafish (Danio rerio) by the method of rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). Zebrafish P-selectin cDNA is 2,800 bp and encodes a put...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms11114618
更新日期:2010-11-17 00:00:00
abstract::Pancreatic cancer (PC) is a highly lethal malignancy due to the cancer routinely being diagnosed late and having a limited response to chemotherapy. Pancreatic ductal adenocarcinoma (PDAC) is the most common form of pancreatic malignant tumor, representing more than 85% of all pancreatic cancers. In the present study,...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21124473
更新日期:2020-06-23 00:00:00
abstract::Enzymatic activation of irinotecan (CPT-11) is due to carboxylesterase (CES), and its pharmacological behavior is influenced by drug resistance-related proteins. We previously reported that the clinical response and prognosis of metastatic colorectal cancer (mCRC) patients did not differ in tumors with different thymi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms150915767
更新日期:2014-09-05 00:00:00
abstract::Benign prostate hyperplasia (BPH) is one of the most common urological problems in mid-aged to elderly men. Risk factors of BPH include family history, obesity, type 2 diabetes, and high oxidative stress. The main medication classes for BPH management are alpha blockers and 5α-reductase inhibitors. However, these conv...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19041137
更新日期:2018-04-10 00:00:00
abstract::The aim of this study was to investigate the chemical composition of acetone extracts of the lichens Toninia candida and Usnea barbata and in vitro antioxidant, antimicrobial, and anticancer activities of these extracts together with some of their major metabolites. The chemical composition of T. candida and U. barbat...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms131114707
更新日期:2012-11-12 00:00:00
abstract::Phytoalexins are antimicrobial substances of low molecular weight produced by plants in response to infection or stress, which form part of their active defense mechanisms. Starting in the 1950's, research on phytoalexins has begun with biochemistry and bio-organic chemistry, resulting in the determination of their st...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms140714136
更新日期:2013-07-08 00:00:00