Inhibitory effect of intraterminal lithium on asynchronous release of excitatory quanta induced by veratridine in nerve-muscle synapses of crayfish.

Abstract:

:Crayfish muscle fibres were voltage-clamped at E = -80 mV membrane potential and superfused for about 10 min with Li+ saline (Na+ replaced by Li+) which contained picrotoxin to block inhibitory post-synaptic currents. Addition of veratridine (100 mumol/l) caused intense fluctuations in the voltage clamp current within 20-60 s due to vigorous asynchronous quantal release of excitatory transmitter from the nerve terminals distributed over the muscle fibre surface. Most likely, this quantal release resulted from loading the nerve terminals with Li+ via voltage-gated Na+ channels activated by veratridine. However, in the presence of Li+ quantal release was only transient; the quantal release rate, ñ, attained a maximum of congruent to 10,000 quanta/s and then declined exponentially with tau congruent to 10 to 20 s. Removal of Li+ and reapplication of normal Na+ increased ñ a second time. The amount of quanta released in the presence of Na+ was about an order of magnitude larger than that released previously in the presence of Li+. In preparations pretreated with Li+ superfusate for t greater than 45 min no marked quantal release could be elicited by veratridine. The experiments suggest an inhibitory effect of intraterminal Li+ on the quantal release process.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Finger W,Martin C

doi

10.1016/0304-3940(87)90225-4

subject

Has Abstract

pub_date

1987-12-16 00:00:00

pages

113-7

issue

1-2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(87)90225-4

journal_volume

83

pub_type

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