Lucifer Yellow labeling of embryonic chick retinal amacrine cells in vitro.

Abstract:

:Lucifer Yellow (LY) selectively labels embryonic chick amacrine cells from days 11 until 14 in vivo. Its usefulness as an in vitro marker was investigated. In vivo labeling and subsequent culturing was not possible due to dye leakage. Neurons, however, could be labeled at various times in vitro. The number of neurons labeled with LY in vitro was consistent with the number of neurons expected to be labeled and increased when selected areas of the retina known to be rich in LY-labeled neurons were used in culturing. Neurons plated at times when labeling was not found in vivo (Embryonic day 8, E8) began to label only at times that were equivalent to times when labeling was found in vivo (E10-E11). This suggests that the selectivity of labeling is preserved in vitro and that LY can be used as an in vitro marker for retinal amacrine cells.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Zeevalk GD,Hyndman AG

doi

10.1016/0304-3940(86)90341-1

subject

Has Abstract

pub_date

1986-03-14 00:00:00

pages

275-80

issue

3

eissn

0304-3940

issn

1872-7972

pii

0304-3940(86)90341-1

journal_volume

64

pub_type

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