Microdialysis studies on cortical noradrenaline release: basic characteristics, significance of extracellular calcium and massive post-mortem increase.

Abstract:

:Extracellular noradrenaline was measured on-line in the cerebral cortex of the freely moving rat by microdialysis coupled to high performance liquid chromatography and electrochemical detection. High potassium concentrations in the perfusion fluid led to a strong increase, whereas tetrodotoxin led to almost undetectable levels of noradrenaline. This shows that noradrenaline in the dialysate was directly derived from active neuronal release. Noradrenaline levels were sensitive to calcium concentrations in the perfusion fluid: from 10% in calcium-free medium to 200% for 10 mM calcium. An overdose of anesthetic caused a 60-fold increase of noradrenaline post-mortem.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

van Veldhuizen MJ,Feenstra MG,Boer GJ,Westerink BH

doi

10.1016/0304-3940(90)90841-v

subject

Has Abstract

pub_date

1990-11-13 00:00:00

pages

233-6

issue

2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(90)90841-V

journal_volume

119

pub_type

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