Neuronal morphological change of size-sieved stem cells induced by neurotrophic stimuli.

Abstract:

:Size-sieved stem cells (SSCs) derived from human bone marrow have the ability to differentiate into bone, fat and cartilage. SSCs can differentiate into active neural cells after exposure to antioxidant agents. The aim of the present study is to understand if SSCs can be stimulated to differentiate into neurons in response to neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), pituitary adenylate cyclase-activating polypeptide (PACAP) and dibutyryl cAMP (dbcAMP). SSCs in a serum-free medium transform from a fibroblastic-like form to a multipolar morphology. Treatment of SSCs with GDNF, PACAP, and dbcAMP increased the production of neurofilament light protein (NF-L) and a cytoskeleton protein-alpha-tubulin. Examination of a vesicle protein-synapsin-1 or a neuronal progenitor marker-internexin in SSCs indicated that treatment with GDNF, PACAP, and dbcAMP further elongated cell processes and increased process branching. The findings indicate that neurotrophic signaling and cAMP-dependent signaling might promote the neuronal differentiation of SSCs.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Tzeng SF,Tsai MJ,Hung SC,Cheng H

doi

10.1016/j.neulet.2004.05.117

keywords:

subject

Has Abstract

pub_date

2004-08-26 00:00:00

pages

23-8

issue

1

eissn

0304-3940

issn

1872-7972

pii

S0304394004006470

journal_volume

367

pub_type

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