Abstract：
:In this paper, delivery devices for nanosecond pulsed electric field exposure of biological samples in direct contact with electrodes or isolated are presented and characterized. They are based on a modified electroporation cuvette and two transverse electromagnetic cells (TEM cells). The devices were used to apply pulses with high intensity (4.5 kV) and short durations (3 and 13 ns). The delivery devices were electromagnetically characterized in the frequency and time domains. Field intensities of around 5, 0.5, and 12 MV m-1 were obtained by numerical simulations of the biological sample positioned in the three delivery devices. Two delivery systems had a homogenous electric field spatial distribution, and one was adapted to permit a highly localized exposure in the vicinity of a needle. Experimental biological investigations were carried out at different field intensities for five cancer cell lines. The results using flow cytometry showed that cells kept polarized mitochondrial membrane but lost plasma membrane integrity following a dose-response trend after exposure to different electric field intensities. Certain cell types (U87, MCF7) showed higher sensitivities to nsPEFs than other lines tested.
journal_name
Med Biol Eng Computjournal_title
Medical & biological engineering & computingauthors
Soueid M,Dobbelaar MCF,Bentouati S,Bardet SM,O'Connor RP,Bessières D,Paillol J,Leveque P,Arnaud-Cormos Ddoi
10.1007/s11517-017-1676-0subject
Has Abstractpub_date
2018-01-01 00:00:00pages
85-97issue
1eissn
0140-0118issn
1741-0444pii
10.1007/s11517-017-1676-0journal_volume
56pub_type
杂志文章abstract：:Remote patient monitoring should reduce mortality rates, improve care, and reduce costs. We present an overview of the available technologies for the remote monitoring of chronic obstructive pulmonary disease (COPD) patients, together with the most important medical information regarding COPD in a language that is ada...
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