Abstract:
:An ontogenetic study of the effect of various neurohormones and other activators on adenylate cyclase systems was carried out using cultures of cells from 15-d-old embryonic mouse brain. Dopamine stimulated the enzyme activity at earlier culture ages (i.e. 4 and 10 d) but had little stimulatory effect at later ages (i.e. 20 and 33 d). Further, this stimulation at the earlier ages was blocked by the dopaminergic blocker, fluphenazine, but not by alpha and beta-adrenergic antagonists. In contrast to dopamine, isoproterenol (a beta-adrenergic agonist) had little stimulatory effect at earlier ages, but its ability to stimulate cyclase activity increased with age. This increase in all age groups was blocked by propranolol (a beta-adrenergic antagonist). Epinephrine-sensitive enzyme activity showed a steady increase with age, which could be blocked with propranolol except in 4-d-old cultures, where it was blocked instead by fluphenazine. Because the cultures are relatively enriched in neurons at earlier ages and in glia in later ages, the results suggest a predominantly neuronal localization for the dopamine sensitive adenylate cyclases and a glial localization of the isoproterenol and epinephrine sensitive adenylate cyclases. Histamine, serotonin, calcium/calmodulin and chloroadenosine were either only slightly or not at all stimulatory.
journal_name
Biosci Repjournal_title
Bioscience reportsauthors
Shanker G,Pieringer RAdoi
10.1007/BF01117073subject
Has Abstractpub_date
1985-07-01 00:00:00pages
601-8issue
7eissn
0144-8463issn
1573-4935journal_volume
5pub_type
杂志文章abstract::Conflicting opinions were recently expressed concerning the possible effect of alpha 2-adrenergic agonists upon cyclic AMP production in pancreatic islets. In the present study, clonidine inhibited glucose-induced insulin release from rat pancreatic islets, this inhibitory effect being abolished by idazoxan. Clonidine...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01122227
更新日期:1984-06-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01136856
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journal_title:Bioscience reports
pub_type: 杂志文章
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20190083
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journal_title:Bioscience reports
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更新日期:1996-02-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20191908
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
pub_type: 杂志文章
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更新日期:1984-11-01 00:00:00
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01114972
更新日期:1986-11-01 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20170695
更新日期:2017-07-27 00:00:00
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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更新日期:2018-09-07 00:00:00
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journal_title:Bioscience reports
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更新日期:2016-04-05 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20201539
更新日期:2021-01-29 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20192345
更新日期:2020-01-31 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20171234
更新日期:2018-04-13 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01116383
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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doi:10.1042/BSR20190337
更新日期:2020-10-30 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1042/BSR20181794
更新日期:2019-07-25 00:00:00
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journal_title:Bioscience reports
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更新日期:2019-09-06 00:00:00
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journal_title:Bioscience reports
pub_type: 杂志文章,评审
doi:10.1042/BSR20170204
更新日期:2017-07-04 00:00:00
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journal_title:Bioscience reports
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doi:10.1042/BSR20170203
更新日期:2018-04-27 00:00:00
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journal_title:Bioscience reports
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journal_title:Bioscience reports
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doi:10.1042/BSR20180367
更新日期:2018-05-22 00:00:00
abstract::Sendai virus envelopes (SVE) were isolated from Sendai virus particles by Triton X-100 solubilization and ultracentrifugation. The envelopes were reconstituted in the presence of the fluorescent dye calcein by gradual removal of the detergent with Bio-beads SM-2. The internal volume of reconstituted Sendai virus envel...
journal_title:Bioscience reports
pub_type: 杂志文章
doi:10.1007/BF01121911
更新日期:1984-07-01 00:00:00