Abstract:
:Human cytomegalovirus (HCMV) is a widely distributed herpesvirus that causes significant morbidity in immunocompromised hosts. Inhibitors of viral DNA replication are available, but adverse effects limit their use. Alternative antiviral strategies may include inhibition of entry. We show that soluble derivatives of the platelet-derived growth factor receptor alpha (PDGFR-alpha), a putative receptor of HCMV, can inhibit HCMV infection of various cell types. A PDGFR-alpha-Fc fusion protein binds to and neutralizes cell-free virus particles at an EC50 of 10-30 ng/ml. Treatment of particles reduced both attachment to and fusion with cells. In line with the latter, PDGFR-alpha-Fc was also effective when applied postattachment. A peptide scan of the extracellular domain of PDGFR-alpha identified a 40mer peptide that inhibits infection at an EC50 of 1-2 nmol/ml. Both, peptide and fusion protein, were effective against various HCMV strains and are hence promising candidates for the development of novel anti-HCMV therapies.
journal_name
PLoS Pathogjournal_title
PLoS pathogensauthors
Stegmann C,Hochdorfer D,Lieber D,Subramanian N,Stöhr D,Laib Sampaio K,Sinzger Cdoi
10.1371/journal.ppat.1006273subject
Has Abstractpub_date
2017-04-12 00:00:00pages
e1006273issue
4eissn
1553-7366issn
1553-7374pii
PPATHOGENS-D-16-01778journal_volume
13pub_type
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