Abstract：
:Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomelo does not carry any foreign DNA sequences but only carries a deletion that is indistinguishable from naturally occurring mutations. We also present evidence for CRISPR/Cas9 being a highly precise tool, as we did not detect off-target mutations in Tomelo. Using our pipeline, mutations can be readily introduced into elite or locally adapted tomato varieties in less than a year with relatively minimal effort and investment.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Nekrasov V,Wang C,Win J,Lanz C,Weigel D,Kamoun Sdoi
10.1038/s41598-017-00578-xsubject
Has Abstractpub_date
2017-03-28 00:00:00pages
482issue
1issn
2045-2322pii
10.1038/s41598-017-00578-xjournal_volume
7pub_type
杂志文章abstract：:MicroRNAs (miRNAs) are important effectors in mediating host-pathogen interaction. In this report, coelomocytes miRNA libraries of three Japanese sea cucumbers Apostichopus japonicus were built by Illumina(®) Hiseq2000 from different time points after lipopolysaccharide challenge (at time 0 h, 6 h and 12 h). The clean...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17763
更新日期：2015-12-04 00:00:00
abstract：:Activation of G protein-gated inwardly rectifying potassium (GIRK) channels leads to a hyperpolarization of the neuron's membrane potential, providing an important component of inhibition in the brain. In addition to the canonical G protein-activation pathway, GIRK channels are activated by small molecules but less is...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04681-x
更新日期：2017-07-04 00:00:00
abstract：:Soil microorganisms play a crucial role in the biogeochemical cycling of nutrient elements and maintaining soil health. We aimed to investigate the response of bacteria communities to organic farming over different crops (rice, tea and vegetable) along the middle and lower reaches of the Yangtze River of China. Compar...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35046
更新日期：2016-10-11 00:00:00
abstract：:We report on a new colorimetric DNA detection method that takes advantage of the power of polymerase chain reaction (PCR) and the simplicity of the classic litmus test. The strategy makes use of a modified set of primers for PCR to facilitate ensuing manipulations of resultant DNA amplicons: their tagging with urease ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03009-z
更新日期：2017-06-08 00:00:00
abstract：:The G-protein coupled, protease-activated receptor 1 (PAR1) is a membrane protein expressed in astrocytes. Fine astrocytic processes are in tight contact with neurons and blood vessels and shape excitatory synaptic transmission due to their abundant expression of glutamate transporters. PAR1 is proteolytically-activat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43606
更新日期：2017-03-03 00:00:00
abstract：:The aim of this study is to explore the differentially expressed lncRNAs, which may have potential biological function and diagnostic value in colorectal cancer (CRC). Through integrated data mining, we finally identified nine differentially expressed lncRNAs and their potential mRNA targets. After a series of bioinfo...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep46572
更新日期：2017-04-13 00:00:00
abstract：:The sedimentary record, and associated micropalaeontological proxies, is one tool that has been employed to quantify a region's tropical cyclone history. Doing so has largely relied on the identification of allochthonous deposits (sediments and microfossils), sourced from deeper water and entrained by tropical cyclone...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24846
更新日期：2016-04-29 00:00:00
abstract：:As fine particle (FP) pollution is harmful to humans, previous studies have focused on the mechanisms of FP removal by forests. The current study aims to compare the FP removal capacities of urban forests and wetlands on the leaf, canopy, and landscape scales. Water washing and scanning electron microscopy are used to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46214
更新日期：2017-04-10 00:00:00
abstract：:The mandibular proximal ramus segments should be moved and rotated during orthognathic surgery-based skeletofacial reconstruction for the correction of challenging patients with facial asymmetry and malocclusion, but quantitative data regarding this rotation were not sufficient to date. This 3D computer-assisted study...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49946-9
更新日期：2019-09-16 00:00:00
abstract：:The Tcf/Lef family of transcription factors mediates the Wnt/β-catenin pathway that is involved in a wide range of biological processes, including vertebrate embryogenesis and diverse pathogenesis. Post-translational modifications, including phosphorylation, sumoylation and acetylation, are known to be important for t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42590
更新日期：2017-02-15 00:00:00
abstract：:Sucralose is a widely-used artificial high-intensity sweetener. Although doubts have been raised about the safety of sucralose by several researchers, it can still be found in a broad range of foods and beverages worldwide, including in baked goods. Sucralose may decompose at high temperatures, and participate in chlo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02946
更新日期：2013-10-15 00:00:00
abstract：:In order to make full use of the impact of internal and external factors on the performance of title catalyst for ethyl benzene oxidation, the key internal influencing factors on the catalytic performance were modulated by coordinating and grafting manganese porphyrin to mesoporous and macroporous chitosan, and the im...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70210-y
更新日期：2020-08-24 00:00:00
abstract：:Horizontal gene transfer (HGT) is a main mechanism of bacterial evolution endowing bacteria with new genetic traits. The transfer of mobile genetic elements such as plasmids (conjugation) requires the close proximity of cells. HGT between genetically distinct bacteria largely depends on cell movement in water films, w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36390
更新日期：2016-11-04 00:00:00
abstract：:Anti-mucin1 (MUC1) antibodies have long been used clinically in cancer diagnosis and therapy and specific bindings of some of them are known to be dependent on the differential glycosylation of MUC1. However, a systematic comparison of the binding specificities of anti-MUC1 antibodies was not previously conducted. Her...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53052-1
更新日期：2019-11-12 00:00:00
abstract：:In this study, real-time and in-situ permittivity measurements under intense microwave electromagnetic fields are proposed as a powerful technique for the study of microwave-enhanced thermal processes in materials. In order to draw reliable conclusions about the temperatures at which transformations occur, we address ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47296-0
更新日期：2019-07-25 00:00:00
abstract：:For scanning x-ray microscopy, many attempts have been made to image the phase contrast based on a concept of the beam being deflected by a specimen, the so-called differential phase contrast imaging (DPC). Despite the successful demonstration in a number of representative cases at moderate spatial resolutions, these ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01307
更新日期：2013-01-01 00:00:00
abstract：:Broadband on-chip optical frequency combs (OFCs) are important for expanding the functionality of photonic integrated circuits. Here, we demonstrate a huge local optical nonlinearity enhancement using graphene. A waveguide is decorated with graphene by precisely manipulating graphene's area and position. Our approach ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45520
更新日期：2017-04-12 00:00:00
abstract：:Many tissues exhibit subatmospheric interstitial pressures under normal physiologic conditions. The mechanisms by which the lymphatic system extracts fluid from these tissues against the overall pressure gradient are unknown. We address this important physiologic issue by combining experimental measurements of contrac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11599-x
更新日期：2017-09-21 00:00:00
abstract：:The growth of epitaxial Si nanowires by a metal-catalyst-free process has been investigated as an alternative to the more common metal-catalyzed vapor-liquid-solid process. The well-aligned Si nanowires are successfully grown on a (111)-oriented Si substrate without any metal catalysts by a thermal treatment using sil...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30608
更新日期：2016-07-28 00:00:00
abstract：:Oligomers populated during the early amyloid aggregation process are more toxic than mature fibrils, but pinpointing the exact toxic species among highly dynamic and heterogeneous aggregation intermediates remains a major challenge. β-barrel oligomers, structurally-determined recently for a slow-aggregating peptide de...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28649-7
更新日期：2018-07-09 00:00:00
abstract：:Atomistic molecular dynamics simulations were performed with 13 non-peptidic neurotransmitters (NTs) in three different membrane environments. The results provide compelling evidence that NTs are divided into membrane-binding and membrane-nonbinding molecules. NTs adhere to the postsynaptic membrane surface whenever t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19345
更新日期：2016-01-19 00:00:00
abstract：:Fatigue is a very common non-motor symptom in Parkinson disease (PD) patients. It included physical fatigue and mental fatigue. The potential mechanisms of mental fatigue involving serotonergic dysfunction and abnormal iron metabolism are still unknown. Therefore, we evaluated the fatigue symptoms, classified PD patie...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-016-0018-z
更新日期：2016-12-21 00:00:00
abstract：:Unimpaired readers process words incredibly fast and hence it was assumed that top-down processing, such as predicting upcoming words, would be too slow to play an appreciable role in reading. This runs counter the major postulate of the predictive coding framework that our brain continually predicts probable upcoming...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08432
更新日期：2015-02-13 00:00:00
abstract：:Geographical origin determination of white rice has become the major issue of food industry. However, there is still lack of a high-throughput method for rapidly and reproducibly differentiating the geographical origins of commercial white rice. In this study, we developed a method that employed lipidomics and deep le...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08892-0
更新日期：2017-08-17 00:00:00
abstract：:Accurate detection of accelerometer non-wear time is crucial for calculating physical activity summary statistics. In this study, we evaluated three epoch-based non-wear algorithms (Hecht, Troiano, and Choi) and one raw-based algorithm (Hees). In addition, we performed a sensitivity analysis to provide insight into th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-62821-2
更新日期：2020-04-03 00:00:00
abstract：:The detection and assessment of leakage in retinal fluorescein angiogram images is important for the management of a wide range of retinal diseases. We have developed a framework that can automatically detect three types of leakage (large focal, punctate focal, and vessel segment leakage) and validated it on images fr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep10425
更新日期：2015-06-01 00:00:00
abstract：:Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and the EV-free secretome to homeostasis and disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates of EVs and free secreted...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15717-7
更新日期：2017-11-10 00:00:00
abstract：:There is a scarcity of data of zinc transporter-8 autoantibody (ZnT8A) on mixed populations such as Brazilian. Therefore, we evaluated the relevance of ZnT8A for type 1 diabetes (T1D) diagnosis and the role of ZnT8 coding gene (SLC30A8) in T1D predisposition. Patients with T1D (n = 629; diabetes duration = 11 (6-16) y...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00307-4
更新日期：2017-03-16 00:00:00
abstract：:A correction has been published and is appended to both the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-37591-7
更新日期：2019-01-22 00:00:00
abstract：:Platinum resistance is one of the most challenging problems in ovarian cancer treatment. High-throughput functional siRNA screening identified tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE-1) as a gene that confers cells resistant to cisplatin. Conversely enforced over-expression of TIE-1 was va...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31069-2
更新日期：2018-09-04 00:00:00
