Abstract：
:Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Burg L,Zhang K,Bonawitz T,Grajevskaja V,Bellipanni G,Waring R,Balciunas Ddoi
10.1038/srep36986subject
Has Abstractpub_date
2016-11-28 00:00:00pages
36986issn
2045-2322pii
srep36986journal_volume
6pub_type
杂志文章abstract：:CSNK2B, which encodes the beta subunit of casein kinase II (CK2), plays an important role in neuron morphology and synaptic transmission. Variants in CSNK2B associated with epilepsy and/or intellectual disability (ID)/developmental delay (DD) have been reported in five cases only. Among the 816 probands suspected here...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53484-9
更新日期：2019-11-29 00:00:00
abstract：:The metabolic health effects of vitamin A and E nutritional status in early life are largely unknown. We assessed whether vitamin A and vitamin E nutritional status may affect circulating leptin, adiponectin, insulin-like growth factor (IGF)-I and IGF-II levels in early life in humans. In a singleton birth cohort (n =...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18531-3
更新日期：2018-01-08 00:00:00
abstract：:Early treatment of single sided deafness in children has been recommended to protect from neurodevelopmental preference for the better hearing ear and from social and educational deficits. A fairly homogeneous group of five young children (≤3.6 years of age) with normal right sided hearing who received a cochlear impl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17129-z
更新日期：2017-12-04 00:00:00
abstract：:The near infrared (NIR) absorption and average particle size of gold nanostars (GNSs) can be precisely controlled by varying the molar ratios of cucurbit[7]urils (CB[7]) and GNSs in aqueous solution. GNSs modified with CB[7] achieved high cargo loading with thermally activated release upon the NIR laser irradiation. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22239
更新日期：2016-02-26 00:00:00
abstract：:We report here the design and multiple functions of a new hierarchical nanotheronostic platform consisting of an upconversion nanoparticle (UCNP) core: shell with an additional mesoporous silica (mSiO2) matrix load shell containing sealed, high concentration of ICG molecules. We demonstrate that this UCNP@mSiO2-ICG na...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-16016-x
更新日期：2017-11-16 00:00:00
abstract：:Injury to the external branch of the superior laryngeal nerve (eSLN) can cause a hoarse or weak voice with dysergia of the cricothyroid. The present study provided the topographic information of the eSLN in the Asian and verified anatomical validity of the landmarks previously recruited to localize the eSLN. Thirty sp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15070-9
更新日期：2017-11-02 00:00:00
abstract：:Malaria is among the major threats to global health with the main burden of disease being in rural areas of developing countries where accurate diagnosis based on non-invasive samples is in high demand. We here present a novel molecular assay for detection of malaria parasites based on technology that may be adapted f...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22378-7
更新日期：2018-03-07 00:00:00
abstract：:Identification of novel photosynthetic proteins is important for understanding and improving photosynthetic efficiency. Synergistically, genome neighborhood can provide additional useful information to identify photosynthetic proteins. We, therefore, expected that applying a computational approach, particularly machin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64053-w
更新日期：2020-04-28 00:00:00
abstract：:We propose to use Rabi oscillation as a probe to view the fractional Josepshon relation (FJR) associated with Majorana bound states (MBSs) expected in one-dimensional topological superconductors. The system consists of a quantum dot (QD) and an rf-SQUID with MBSs at the Josephson junction. Rabi oscillations between en...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11686
更新日期：2015-07-08 00:00:00
abstract：:We propose the realization of photonic circuits whose dynamics is governed by advanced-retarded differential equations. Beyond their mathematical interest, these photonic configurations enable the implementation of quantum feedback and feedforward without requiring any intermediate measurement. We show how this protoc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42933
更新日期：2017-02-23 00:00:00
abstract：:Hollow fiber composite NF membranes with improved anti-fouling property and water flux were prepared via interfacial polymerizationand layer-by-layer method using polyethylenimine (PEI), isophthaloyl dichloride (IPC), and β-cyclodextrin (β-CD). The chemical structures and the morphologies of the resultant NF membranes...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48908-5
更新日期：2019-08-27 00:00:00
abstract：:Double-strand breaks (DSBs) are the most lethal DNA damages induced by ionising radiation (IR) and their efficient repair is crucial to limit genomic instability. The cellular DSB response after low IR doses is of particular interest but its examination requires the analysis of high cell numbers. Here, we present an a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35660-5
更新日期：2018-11-23 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-58622-2
更新日期：2020-01-28 00:00:00
abstract：:In the present work, we demonstrated the biosynthesis of silver nanoparticles (AgNPs) by highly stable, economic and eco-friendly method using leaf extract of Terminalia arjuna (T. arjuna) and employing as a catalyst for the degradation of methyl orange (MO), methylene blue (MB), congo red (CR) and 4- nitrophenol (4-N...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66851-8
更新日期：2020-06-15 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep34617. ...
journal_title：Scientific reports
pub_type： 杂志文章,已发布勘误
doi：10.1038/srep42267
更新日期：2017-09-27 00:00:00
abstract：:Age-hardening in Al alloys has been used for over a century to improve its mechanical properties. However, the lack of direct observation limits our understanding of the dynamic nature of the evolution of nanoprecipitates during age-hardening. Using in-situ (scanning) transmission electron microscopy (S/TEM) while hea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02081-9
更新日期：2017-05-19 00:00:00
abstract：:Atomoxetine is a norepinephrine reuptake inhibitor indicated in the treatment of attention-deficit/hyperactivity disorder. It is primarily metabolized by CYP2D6 to its equipotent metabolite, 4-hydroxyatomoxetine, which promptly undergoes further glucuronidation to an inactive 4-HAT-O-glucuronide. Clinical trials have ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30841-8
更新日期：2018-08-17 00:00:00
abstract：:Elastography plays a key role in characterizing soft media such as biological tissue. Although this technology has found widespread use in both clinical diagnostics and basic science research, nearly all methods require direct physical contact with the object of interest and can even be invasive. For a number of appli...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38967
更新日期：2016-12-23 00:00:00
abstract：:A tetrameric N-acetyl galactosaminyl (GalNAc) peptidomimetic was constructed by N-acetylation of repeating proline-based hydroxamic acid units, followed by a convergent 'click chemistry' coupling. This novel glycopeptidomimetic was determined to effectively antagonize the interaction between a transmembrane hepatic le...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05513
更新日期：2014-07-01 00:00:00
abstract：:Chronic kidney disease (CKD) patients have many affected physiological pathways. Variations in the genes regulating these pathways might affect the incidence and predisposition to this disease. A total of 722 Spanish adults, including 548 patients and 174 controls, were genotyped to better understand the effects of ge...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56695-2
更新日期：2020-01-10 00:00:00
abstract：:Photodynamic therapy (PDT) is an efficacious treatment for some types of cancers. However, PDT-induced tumor hypoxia as a result of oxygen consumption and vascular damage can reduce the efficacy of this therapy. Measuring and monitoring intrinsic and PDT-induced tumor hypoxia in vivo during PDT is of high interest for...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31551
更新日期：2016-08-22 00:00:00
abstract：:The extended Hückel (eH) tight-binding method has historically been prized for its computational ease and intuitive chemical clarity. However, its lack of quantitative predictiveness has prevented the eH method from being used as a tool for rapidly screening materials for desired electronic properties. In this work, w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28864-2
更新日期：2018-07-12 00:00:00
abstract：:Using analytical expressions for the magnetization textures of thin submicron-sized magnetic cylinders in vortex state, we derive closed-form algebraic expressions for the ensuing small-angle neutron scattering (SANS) cross sections. Specifically, for the perpendicular and parallel scattering geometries, we have compu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25055
更新日期：2016-04-26 00:00:00
abstract：:Increasing life expectancy and a growing share of older people around the world spotlight the issue of health during additional years of life. Research on trends of proportions of older people with activity limitations for low and middle income countries is sparse. We use data from the World Health Survey and the UN W...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67166-4
更新日期：2020-06-26 00:00:00
abstract：:Mitochondrial dysfunction contributes to cell death after cerebral ischemia/reperfusion (I/R) injury. Cannabinoid CB1 receptor is expressed in neuronal mitochondrial membranes (mtCB1R) and involved in regulating mitochondrial functions under physiological conditions. However, whether mtCB1R affords neuroprotection aga...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12440
更新日期：2015-07-28 00:00:00
abstract：:Lipidised analgesic peptide prodrugs self-assemble into peptide nanofibers; with the nanofiber morphology protecting the peptide from plasma degradation and improving therapeutic efficacy. Extending this learning, we hypothesised that a self-assembling lipidized peptide arginine vasopressin (AVP) receptor agonist, tha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-64070-9
更新日期：2020-04-29 00:00:00
abstract：:Coronaviruses may exert severely negative effects on the mortality and morbidity of birds and mammals including humans and domestic animals. Most recently CoVID-19 has killed about half million people (27th of June, 2020). Susceptibility to this disease appears to differ markedly across different societies but the fac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69351-x
更新日期：2020-07-27 00:00:00
abstract：:Guanylate binding proteins (GBPs) belongs to the interferons (IFNs) induced guanylate-binding protein family (Guanosine triphosphatases, GTPases) consisting of seven homologous members, termed GBP1 to GBP7. We used multidimensional survey ways to explore GBPs expression, regulation, mutations, immune infiltration and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63246-7
更新日期：2020-04-08 00:00:00
abstract：:Measurement of intracranial aneurysm wall motion may refine the current rupture risk estimation. A golden standard for measuring aneurysm pulsation is lacking. The aim is to evaluate magnitudes of aneurysm pulsation as published in current literature. Embase and PubMed were searched for publications containing quantit...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81753-z
更新日期：2021-01-26 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-60629-8
更新日期：2020-02-25 00:00:00
