Abstract：
BACKGROUND:The microbial community in anaerobic digestion is mainly monitored by means of DNA-based methods. This may lead to incorrect interpretation of the community parameters, because microbial abundance does not necessarily reflect activity. In this research, the difference between microbial community response on DNA (total community) and RNA (active community) based on the 16S rRNA (gene) with respect to salt concentration and response time was evaluated. RESULTS:The application of higher NaCl concentrations resulted in a decrease in methane production. A stronger and faster response to salt concentration was observed on RNA level. This was reflected in terms of microbial community composition and organization, as richness, evenness, and overall diversity were differentially impacted. A higher divergence of community structure was observed on RNA level as well, indicating that total community composition depends on deterministic processes, while the active community is determined by stochastic processes. Methanosaeta was identified as the most abundant methanogen on DNA level, but its relative abundance decreased on RNA level, related to salt perturbation. CONCLUSIONS:This research demonstrated the need for RNA-based community screening to obtain reliable information on actual community parameters and to identify key species that determine process stability.
journal_name
Biotechnol Biofuelsjournal_title
Biotechnology for biofuelsauthors
De Vrieze J,Regueiro L,Props R,Vilchez-Vargas R,Jáuregui R,Pieper DH,Lema JM,Carballa Mdoi
10.1186/s13068-016-0652-5subject
Has Abstractpub_date
2016-11-09 00:00:00pages
244issn
1754-6834pii
652journal_volume
9pub_type
杂志文章abstract：BACKGROUND:The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for their commercial application. In this study, we quantitatively compared the lignocellulolytic enzyme p...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-172
更新日期：2013-11-29 00:00:00
abstract：Background:Lignin is a heterogeneous polymer representing a renewable source of aromatic and phenolic bio-derived products for the chemical industry. However, the inherent structural complexity and recalcitrance of lignin makes its conversion into valuable chemicals a challenge. Natural microbial communities produce bi...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1073-4
更新日期：2018-03-22 00:00:00
abstract：BACKGROUND:Complete conversion of the major sugars of biomass including both the C5 and C6 sugars is critical for biofuel production processes. Several inhibitory compounds like acetate, hydroxymethylfurfural (HMF), and furfural are produced from the biomass pretreatment process leading to 'hydrolysate toxicity,' a maj...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0233-z
更新日期：2015-03-31 00:00:00
abstract：BACKGROUND:Microbial lipid production using renewable feedstock shows great promise for the biodiesel industry. RESULTS:In this study, the ability of a lipid-engineered Yarrowia lipolytica strain JMY4086 to produce lipids using molasses and crude glycerol under different oxygenation conditions and at different inoculu...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0286-z
更新日期：2015-07-25 00:00:00
abstract：BACKGROUND:Coordination of synthesis and assembly of the polymeric components of cell walls is essential for plant growth and development. Given the degree of co-mingling and cross-linking among cell wall components, cellulose organization must be dependent on the organization of other polymers such as lignin. Here we ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0540-z
更新日期：2016-06-17 00:00:00
abstract：BACKGROUND:Furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulose biomass to monomeric sugars, but the response of acetone butanol ethanol (ABE) producing Clostridium beijerinckii NCIMB 8052 to this compound at the molecular level is unknown. To discern the effect o...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-66
更新日期：2013-05-04 00:00:00
abstract：Background:Cyanobacteria have shown promising potential for the production of various biofuels and chemical feedstocks. Synechococcus elongatus UTEX 2973 is a fast-growing strain with pronounced tolerance to high temperatures and illumination. Hence, this strain appears to be ideal for the development of photosynthetic...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1215-8
更新日期：2018-08-04 00:00:00
abstract：BACKGROUND:The filamentous fungus Trichoderma reesei is widely utilized in industry for cellulase production, but its xylanase activity must be improved to enhance the accessibility of lignocellulose to cellulases. Several transcription factors play important roles in this progress; however, nearly all the reported tra...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0878-x
更新日期：2017-08-03 00:00:00
abstract：BACKGROUND:There has been a great deal of interest in fuel productions from lignocellulosic biomass to minimize the conflict between food and fuel use. The bioconversion of xylose, which is the second most abundant sugar present after glucose in lignocellulosic biomass, is important for the development of cost effectiv...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-134
更新日期：2013-09-16 00:00:00
abstract：BACKGROUND:P450 fatty acid decarboxylases represented by the unusual CYP152 peroxygenase family member OleTJE have been receiving great attention recently since these P450 enzymes are able to catalyze the simple and direct production of 1-alkenes for potential applications in biofuels and biomaterials. To gain more mec...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0894-x
更新日期：2017-09-07 00:00:00
abstract：Background:α-l-Arabinofuranosidase (ARA), a debranching enzyme that can remove arabinose substituents from arabinoxylan and arabinoxylooligomers (AXOS), promotes the hydrolysis of the arabinoxylan fraction of biomass; however, the impact of ARA on the overall digestibility of cellulose is controversial. In this study, ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1412-0
更新日期：2019-03-22 00:00:00
abstract：BACKGROUND:n-Butanol can serve as an excellent gasoline substitute. Naturally, it is produced by some Clostridia species which, however, exhibit only limited suitability for industrial n-butanol production. The yeast Saccharomyces cerevisiae would be an ideal host due to its high robustness in fermentation processes. N...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0673-0
更新日期：2016-11-25 00:00:00
abstract：BACKGROUND:Alcohol-to-jet (ATJ) is one of the technical feasible biofuel technologies. It produces jet fuel from sugary, starchy, and lignocellulosic biomass, such as sugarcane, corn grain, and switchgrass, via fermentation of sugars to ethanol or other alcohols. This study assesses the ATJ biofuel production pathway f...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0702-7
更新日期：2017-01-19 00:00:00
abstract：BACKGROUND:Molasses is a dense and saline by-product of the sugar agroindustry. Its high organic content potentially fuels a myriad of renewable products of industrial interest. However, the biotechnological exploitation of molasses is mainly hampered by the high concentration of salts, an issue that is nowadays tackle...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0701-8
更新日期：2017-01-31 00:00:00
abstract：BACKGROUND:Low cost of raw materials and good process yields are necessary for future lignocellulosic biomass biorefineries to be sustainable and profitable. A low cost feedstock will be diverse, changing as a function of seasonality and price and will most likely be available from multiple sources to the biorefinery. ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0414-9
更新日期：2015-12-24 00:00:00
abstract：BACKGROUND:The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes. RESULTS:Trichosporon mycotoxinivorans CLA2 is bioemulsifier-producing yeast strain isolated ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-5-29
更新日期：2012-05-06 00:00:00
abstract：BACKGROUND:Bamboo is potentially an interesting feedstock for advanced bioethanol production in China due to its natural abundance, rapid growth, perennial nature and low management requirements. Liquid hot water (LHW) pretreatment was selected as a promising technology to enhance sugar release from bamboo lignocellulo...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-173
更新日期：2013-11-29 00:00:00
abstract：Background:Cyanobacteria can be metabolically engineered to convert CO2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules. Results:The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobac...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-020-1653-y
更新日期：2020-01-28 00:00:00
abstract：Background:We previously developed several strategies to engineer plants to produce cost-efficient biofuels from plant biomass. Engineered Arabidopsis plants with low xylan and lignin content showed normal growth and improved saccharification efficiency under standard growth conditions. However, it remains to be determ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-018-1196-7
更新日期：2018-07-18 00:00:00
abstract：BACKGROUND:While microbial electrolysis cells (MECs) can simultaneously produce bioelectrochemical hydrogen and treat wastewater, they consume considerable energy to overcome the unfavorable thermodynamics, which is not sustainable and economically feasible in practical applications. This study presents a proof-of-conc...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-015-0305-0
更新日期：2015-08-14 00:00:00
abstract：:[This corrects the article DOI: 10.1186/s13068-018-1097-9.]. ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章,已发布勘误
doi：10.1186/s13068-018-1185-x
更新日期：2018-07-03 00:00:00
abstract：Background:A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solution of renewable routes to chemicals and fuels. Members of the genus Geobacillus are able to grow at...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1540-6
更新日期：2019-08-20 00:00:00
abstract：BACKGROUND:Despite decades of work and billions of dollars of investments in laboratory and pilot plant projects, commercial production of cellulosic ethanol is only now beginning to emerge. Because of: (1)high technical risk coupled with; (2) high capital investment cost relative to ethanol product value, investors ha...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-6-13
更新日期：2013-01-28 00:00:00
abstract：Background:Biomass-derived jet fuel is an alternative jet fuel (AJF) showing promise of reducing the dependence on fossil fuel and greenhouse gas emissions. Hydroprocessed esters and fatty acids (HEFA) concept is also known as one of the pathways for producing bio jet fuel. HEFA fuel was approved by the American Societ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-0945-3
更新日期：2017-11-09 00:00:00
abstract：Background:Interests in renewable fuels have exploded in recent years as the serious effects of global climate change become apparent. Microbial production of high-energy fuels by economically efficient bioprocesses has emerged as an attractive alternative to the traditional production of transportation fuels. Here, we...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-017-1003-x
更新日期：2018-01-08 00:00:00
abstract：BACKGROUND:Recently developed iron cocatalyst enhancement of dilute acid pretreatment of biomass is a promising approach for enhancing sugar release from recalcitrant lignocellulosic biomass. However, very little is known about the underlying mechanisms of this enhancement. In the current study, our aim was to identify...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-4-48
更新日期：2011-11-10 00:00:00
abstract：BACKGROUND:Lipid/carbohydrate content and ratio are extremely important when engineering algal cells for liquid biofuel production. However, conventional methods for such determination and quantification are not only destructive and tedious, but also energy consuming and environment unfriendly. In this study, we first ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0691-y
更新日期：2017-01-03 00:00:00
abstract：BACKGROUND:The production of ethanol and other fuels and chemicals from lignocellulosic materials is dependent of efficient xylose conversion. Xylose fermentation capacity in yeasts is usually linked to xylose reductase (XR) accepting NADH as cofactor. The XR from Scheffersomyces stipitis, which is able to use NADH as ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-016-0570-6
更新日期：2016-08-05 00:00:00
abstract：Background:Black soldier fly (BSF, Hermetia illucens L.) can efficiently degrade organic wastes and transform into a high fat containing insect biomass that could be used as feedstock for biodiesel production. Meanwhile, the molecular regulatory basis of fat accumulation by BSF is still unclear; it is necessary to iden...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/s13068-019-1531-7
更新日期：2019-08-09 00:00:00
abstract：BACKGROUND:Globally, the development of a cost-effective long-term renewable energy infrastructure is one of the most challenging problems faced by society today. Microalgae are rich in potential biofuel substrates such as lipids, including triacylglycerols (TAGs). Some of these algae also biosynthesize small molecule ...
journal_title：Biotechnology for biofuels
pub_type： 杂志文章
doi：10.1186/1754-6834-7-62
更新日期：2014-04-15 00:00:00
